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The map label for this gene is purF [H]

Identifier: 85860900

GI number: 85860900

Start: 3073496

End: 3074908

Strand: Direct

Name: purF [H]

Synonym: SYN_01624

Alternate gene names: 85860900

Gene position: 3073496-3074908 (Clockwise)

Preceding gene: 85860899

Following gene: 85860901

Centisome position: 96.67

GC content: 58.95

Gene sequence:

>1413_bases
ATGGAAACTCAACTATCGGTTATTGAAGACAAACCCCGCGAGGCCTGCGGCGTTTTCGCCGTCTACGGACACCCCGAGGC
CGCGAAGCTCACCTTCTTCGGTCTCTTCGCCCTGCAGCACCGGGGACAGGAAAGCGCCGGCATTGCCGCGTCAGACGGAT
GCATGGTCAGGGCCCATAAAGGAATGGGACTCGCTTCGGAAGTTTTCGGAGAAGAAAATCTGGCCCGCCTTCCCGGCAAT
CTGGCCATCGGCCATGTCCGCTATTCCACGACCGGTTCCTCCACCCTGTCCAATGCCCAGCCCTTCCTGGTTCATCATGG
GGAAGAATATTACGCCCTGGGCCACAACGGCAACCTGATCAACGCCCAGTCGCTGCGCCGGGAGTTGGAGGACTCGGGCT
CCATCTTCCAGTCCACCATGGACAGCGAGGTCATCGTTCACCTCATGGCGCCGCATCTGAAGAAAGGCATTGAACCGGCC
CTGAAAGCCGCCCTCAAGAGGGTGCAGGGGGCTTACAGCATCGTCATGCTGACGCGGAACTCGGTCATCGCGTTCCGCGA
TCCCCGGGGGTTCCGCCCGTTGTCGCTTGGCCGTCTCAATGACGGCTGGGTGGTGGCGTCGGAAACCTGCGCCTTTGATC
TCGTGGGCGCCCAGTATCTGCGGGATGTCCAGCCGGGGGAGATCGTCATCATCAACGAAGTAGGGTTGCACAGCCTGAAG
CCCTTTCCCCGTGTGCCCCACTCCCACTGCATCTTTGAGCTCATCTATTTTGCCCGCCCGGACAGTCAGGTCTTCGGGCA
GAACGTCTATCTCTGCCGGAAGCGGTTCGGCCATGCCCTGGCCCGGGAATACAGCCCCGATGTCGATTTCGTCATGCCCT
TTCCCGATTCGGGCAACTACGCCGCCCTGGGATATTCGGAGGAAAGCGGCTTCCCCTTCGAGATGGGGATGATCCGCAAT
CATTATGTAGGACGGACTTTCATTCAGCCGACACAGCCCATGAGGGATTTTGCGGTGCGGGTGAAGCTCAATCCCGTGAA
ACCTCTCATGGCGGGCAAGAAGATCCTGATCATCGAGGATTCCATCATCCGGGGAACGACCAGCCGCAACCGGGTCCGCT
ACCTCCGGGAAAACGGGGCCAAGGCCATTCATATGGCGATCAGTTGTCCCCCAACCCGGTTTCCCTGCCCCTATGGAATC
GATTTTTCCGCAAAAGGAGAACTCCTTGCCGCCCAGAAAGGTATTGAAGAGGAGATCGCCCGCTTCATCGGACTCGATTC
CATCCATTACCTGAGCCTGGAGGGACTGGTCAGGAGCACGGGAGTGAATCAGGAGGCCTTCTGCCTGGCCTGTTACACGG
GAAAGTATCCTCTGCAGCCACCGGGAGAAATCCAGAAGCTCTGTTTTGAATAG

Upstream 100 bases:

>100_bases
GGTTCTAAAGAAGTTCAGCGTTCAAAGTTGGGACTTCCGGCTGAGAACATGAAACTTTGAACCCGAAACGCTGGAGGATC
CAATCAGGGCATAAAAATTC

Downstream 100 bases:

>100_bases
TGGGACACACCGTATTTTGGAGAGGTGACGATTGGAAATCATTGATTCTCATACCCATTGGGGGCCTTCCGCTGCTCTGG
GCATTTCCGTGACCACAGAG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
METQLSVIEDKPREACGVFAVYGHPEAAKLTFFGLFALQHRGQESAGIAASDGCMVRAHKGMGLASEVFGEENLARLPGN
LAIGHVRYSTTGSSTLSNAQPFLVHHGEEYYALGHNGNLINAQSLRRELEDSGSIFQSTMDSEVIVHLMAPHLKKGIEPA
LKAALKRVQGAYSIVMLTRNSVIAFRDPRGFRPLSLGRLNDGWVVASETCAFDLVGAQYLRDVQPGEIVIINEVGLHSLK
PFPRVPHSHCIFELIYFARPDSQVFGQNVYLCRKRFGHALAREYSPDVDFVMPFPDSGNYAALGYSEESGFPFEMGMIRN
HYVGRTFIQPTQPMRDFAVRVKLNPVKPLMAGKKILIIEDSIIRGTTSRNRVRYLRENGAKAIHMAISCPPTRFPCPYGI
DFSAKGELLAAQKGIEEEIARFIGLDSIHYLSLEGLVRSTGVNQEAFCLACYTGKYPLQPPGEIQKLCFE

Sequences:

>Translated_470_residues
METQLSVIEDKPREACGVFAVYGHPEAAKLTFFGLFALQHRGQESAGIAASDGCMVRAHKGMGLASEVFGEENLARLPGN
LAIGHVRYSTTGSSTLSNAQPFLVHHGEEYYALGHNGNLINAQSLRRELEDSGSIFQSTMDSEVIVHLMAPHLKKGIEPA
LKAALKRVQGAYSIVMLTRNSVIAFRDPRGFRPLSLGRLNDGWVVASETCAFDLVGAQYLRDVQPGEIVIINEVGLHSLK
PFPRVPHSHCIFELIYFARPDSQVFGQNVYLCRKRFGHALAREYSPDVDFVMPFPDSGNYAALGYSEESGFPFEMGMIRN
HYVGRTFIQPTQPMRDFAVRVKLNPVKPLMAGKKILIIEDSIIRGTTSRNRVRYLRENGAKAIHMAISCPPTRFPCPYGI
DFSAKGELLAAQKGIEEEIARFIGLDSIHYLSLEGLVRSTGVNQEAFCLACYTGKYPLQPPGEIQKLCFE
>Mature_470_residues
METQLSVIEDKPREACGVFAVYGHPEAAKLTFFGLFALQHRGQESAGIAASDGCMVRAHKGMGLASEVFGEENLARLPGN
LAIGHVRYSTTGSSTLSNAQPFLVHHGEEYYALGHNGNLINAQSLRRELEDSGSIFQSTMDSEVIVHLMAPHLKKGIEPA
LKAALKRVQGAYSIVMLTRNSVIAFRDPRGFRPLSLGRLNDGWVVASETCAFDLVGAQYLRDVQPGEIVIINEVGLHSLK
PFPRVPHSHCIFELIYFARPDSQVFGQNVYLCRKRFGHALAREYSPDVDFVMPFPDSGNYAALGYSEESGFPFEMGMIRN
HYVGRTFIQPTQPMRDFAVRVKLNPVKPLMAGKKILIIEDSIIRGTTSRNRVRYLRENGAKAIHMAISCPPTRFPCPYGI
DFSAKGELLAAQKGIEEEIARFIGLDSIHYLSLEGLVRSTGVNQEAFCLACYTGKYPLQPPGEIQKLCFE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=39.6449704142012, Blast_Score=340, Evalue=2e-93,
Organism=Escherichia coli, GI1788651, Length=470, Percent_Identity=38.5106382978723, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1790167, Length=201, Percent_Identity=30.8457711442786, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=38.4937238493724, Blast_Score=314, Evalue=6e-86,
Organism=Saccharomyces cerevisiae, GI6323958, Length=455, Percent_Identity=37.8021978021978, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24659598, Length=489, Percent_Identity=39.6728016359918, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24659604, Length=469, Percent_Identity=39.8720682302772, Blast_Score=334, Evalue=8e-92,
Organism=Drosophila melanogaster, GI28573187, Length=494, Percent_Identity=39.4736842105263, Blast_Score=332, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52027; Mature: 52027

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METQLSVIEDKPREACGVFAVYGHPEAAKLTFFGLFALQHRGQESAGIAASDGCMVRAHK
CCCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEC
GMGLASEVFGEENLARLPGNLAIGHVRYSTTGSSTLSNAQPFLVHHGEEYYALGHNGNLI
CCCHHHHHHCCCCHHHCCCCEEEEEEEEECCCCCCCCCCCCEEEECCCEEEEECCCCCEE
NAQSLRRELEDSGSIFQSTMDSEVIVHLMAPHLKKGIEPALKAALKRVQGAYSIVMLTRN
CHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEECC
SVIAFRDPRGFRPLSLGRLNDGWVVASETCAFDLVGAQYLRDVQPGEIVIINEVGLHSLK
CEEEEECCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHCCCCCCEEEEECCCCCCCC
PFPRVPHSHCIFELIYFARPDSQVFGQNVYLCRKRFGHALAREYSPDVDFVMPFPDSGNY
CCCCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCE
AALGYSEESGFPFEMGMIRNHYVGRTFIQPTQPMRDFAVRVKLNPVKPLMAGKKILIIED
EEEECCCCCCCCEEECCHHHCCCCCEECCCCCCCCCEEEEEEECCCCHHHCCCEEEEEEC
SIIRGTTSRNRVRYLRENGAKAIHMAISCPPTRFPCPYGIDFSAKGELLAAQKGIEEEIA
CEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEHHHCCHHHHHH
RFIGLDSIHYLSLEGLVRSTGVNQEAFCLACYTGKYPLQPPGEIQKLCFE
HHHCCCCEEEEEHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
METQLSVIEDKPREACGVFAVYGHPEAAKLTFFGLFALQHRGQESAGIAASDGCMVRAHK
CCCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEC
GMGLASEVFGEENLARLPGNLAIGHVRYSTTGSSTLSNAQPFLVHHGEEYYALGHNGNLI
CCCHHHHHHCCCCHHHCCCCEEEEEEEEECCCCCCCCCCCCEEEECCCEEEEECCCCCEE
NAQSLRRELEDSGSIFQSTMDSEVIVHLMAPHLKKGIEPALKAALKRVQGAYSIVMLTRN
CHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEECC
SVIAFRDPRGFRPLSLGRLNDGWVVASETCAFDLVGAQYLRDVQPGEIVIINEVGLHSLK
CEEEEECCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHCCCCCCEEEEECCCCCCCC
PFPRVPHSHCIFELIYFARPDSQVFGQNVYLCRKRFGHALAREYSPDVDFVMPFPDSGNY
CCCCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCE
AALGYSEESGFPFEMGMIRNHYVGRTFIQPTQPMRDFAVRVKLNPVKPLMAGKKILIIED
EEEECCCCCCCCEEECCHHHCCCCCEECCCCCCCCCEEEEEEECCCCHHHCCCEEEEEEC
SIIRGTTSRNRVRYLRENGAKAIHMAISCPPTRFPCPYGIDFSAKGELLAAQKGIEEEIA
CEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEHHHCCHHHHHH
RFIGLDSIHYLSLEGLVRSTGVNQEAFCLACYTGKYPLQPPGEIQKLCFE
HHHCCCCEEEEEHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]