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The map label for this gene is carA [H]

Identifier: 85860897

GI number: 85860897

Start: 3067426

End: 3068631

Strand: Direct

Name: carA [H]

Synonym: SYN_01627

Alternate gene names: 85860897

Gene position: 3067426-3068631 (Clockwise)

Preceding gene: 85860896

Following gene: 85860898

Centisome position: 96.48

GC content: 58.29

Gene sequence:

>1206_bases
TTGAGAAATAGCCGGCGTTCAAACAATGAGGAACAACACGACATGACCCTGCTGAGAAAACAGAAACCCGCGCTCCTGGT
TCTGGAAGACGGGTGTGTCTTTCGGGGAGAAGCCTTCGCGGGTGAGGGCGAAGTCCTGGGAGAGGTCGTCTTCAATACCG
GGATGACGGGCTACCAGGAGGTTCTGACCGATCCATCCTACAAGGGGCAGATCGTCGCCATGACCTATCCGCTGATCGGC
AACTACGGGATCAATCCCGAGGATATGGAAGCAGCGGGAATTTCTCTGGAGGGGTTTATCGTCCGGGAATATTCGGATTT
CCCGAGTAACTGGCGATCCCGGCAGAGCCTGAAGACCTTTATGGAATCCCAGGGCAAGCTGGGTGTCGAGGGACTGGACT
GCCGCGCCCTGACACGGCGTCTTCGCGTTTCCGGGGCGATGCGCGGCATTCTGACCACGGAAATCCAGGACGTGGAGATT
CTGCTCCAGCGGGTGCGGGCATATCCCGGTCTTGTCGGGCAGGACCTGGTCAGGACCGTAACGTGTCAAGAGCCGTACCG
ATGGGTGCGCGGGAGCGTTCAGCCGGCTGACCGGGACCGGAGGCCGGAGGAAGGCAAGAATCTCCGGGTGGTCGTTCTGG
ATTGCGGCGTCAAATACAATATCCTGCGCTGTCTGGAGGCGGAAGGCTGCGAGGTGATCGTCGTCCCCGCCTGCTCTTCC
GATGAACAGATTCTGTCCTGGTCTCCTGACGGGCTGCTCCTGTCCAACGGTCCGGGCGATCCGGCTGCACTGCCTTATAT
CGTGGCCACCGTCCGGCAATTGCTGGGGAAAGTTCCGATCTTCGGCATCTGCCTGGGACATCAGATCCTGGGACAGGCTG
TCGGCGGCCGCACCGAAAAATTGAAGTTCGGCCATCACGGGATCAATCAACCGGTGAAACACCGCCTGTCGGGCCGGGTG
GAAATCACCTCCCAGAATCACGGATTTGTCGTTGTGCCGGAATCCCTTCCCGCGGATCTGGAAAAAACGCATGACAATCT
CAATGACGGGACATCGGAAGGAATCGCTTATCCGTCTCTGGGGGCGTTCAGCGTCCAGTATCACCCCGAAGCGGCGCCGG
GGCCGCAGGATGCATCCTATCTTTTCAAACAGTTTGTAAAACTAATCACATCAGAGAAGGGAAACAAGCTTAGGACCCCT
TCCTGA

Upstream 100 bases:

>100_bases
ATAGTGATATCAATTATTTAAGCTTCCATCGCCTGATTTTTCCGTCCGTTGACGGTGAGAAGGCAAAAGGGGCCGGAACC
GCCCCGTCGCGGTCTACCTC

Downstream 100 bases:

>100_bases
GACATGAGAGGCTTATCTTGATTCTTATTATTGATTTCGGTTCTCAATACAACCAGCTGATCGCCCGGCGCGTGCGGGAG
CTGGAGGTGTACTGCCAGAT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MRNSRRSNNEEQHDMTLLRKQKPALLVLEDGCVFRGEAFAGEGEVLGEVVFNTGMTGYQEVLTDPSYKGQIVAMTYPLIG
NYGINPEDMEAAGISLEGFIVREYSDFPSNWRSRQSLKTFMESQGKLGVEGLDCRALTRRLRVSGAMRGILTTEIQDVEI
LLQRVRAYPGLVGQDLVRTVTCQEPYRWVRGSVQPADRDRRPEEGKNLRVVVLDCGVKYNILRCLEAEGCEVIVVPACSS
DEQILSWSPDGLLLSNGPGDPAALPYIVATVRQLLGKVPIFGICLGHQILGQAVGGRTEKLKFGHHGINQPVKHRLSGRV
EITSQNHGFVVVPESLPADLEKTHDNLNDGTSEGIAYPSLGAFSVQYHPEAAPGPQDASYLFKQFVKLITSEKGNKLRTP
S

Sequences:

>Translated_401_residues
MRNSRRSNNEEQHDMTLLRKQKPALLVLEDGCVFRGEAFAGEGEVLGEVVFNTGMTGYQEVLTDPSYKGQIVAMTYPLIG
NYGINPEDMEAAGISLEGFIVREYSDFPSNWRSRQSLKTFMESQGKLGVEGLDCRALTRRLRVSGAMRGILTTEIQDVEI
LLQRVRAYPGLVGQDLVRTVTCQEPYRWVRGSVQPADRDRRPEEGKNLRVVVLDCGVKYNILRCLEAEGCEVIVVPACSS
DEQILSWSPDGLLLSNGPGDPAALPYIVATVRQLLGKVPIFGICLGHQILGQAVGGRTEKLKFGHHGINQPVKHRLSGRV
EITSQNHGFVVVPESLPADLEKTHDNLNDGTSEGIAYPSLGAFSVQYHPEAAPGPQDASYLFKQFVKLITSEKGNKLRTP
S
>Mature_401_residues
MRNSRRSNNEEQHDMTLLRKQKPALLVLEDGCVFRGEAFAGEGEVLGEVVFNTGMTGYQEVLTDPSYKGQIVAMTYPLIG
NYGINPEDMEAAGISLEGFIVREYSDFPSNWRSRQSLKTFMESQGKLGVEGLDCRALTRRLRVSGAMRGILTTEIQDVEI
LLQRVRAYPGLVGQDLVRTVTCQEPYRWVRGSVQPADRDRRPEEGKNLRVVVLDCGVKYNILRCLEAEGCEVIVVPACSS
DEQILSWSPDGLLLSNGPGDPAALPYIVATVRQLLGKVPIFGICLGHQILGQAVGGRTEKLKFGHHGINQPVKHRLSGRV
EITSQNHGFVVVPESLPADLEKTHDNLNDGTSEGIAYPSLGAFSVQYHPEAAPGPQDASYLFKQFVKLITSEKGNKLRTP
S

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=381, Percent_Identity=43.3070866141732, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI21361331, Length=394, Percent_Identity=35.2791878172589, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI169790915, Length=394, Percent_Identity=35.2791878172589, Blast_Score=227, Evalue=1e-59,
Organism=Escherichia coli, GI1786215, Length=372, Percent_Identity=47.0430107526882, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1789760, Length=134, Percent_Identity=31.3432835820896, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=384, Percent_Identity=38.8020833333333, Blast_Score=256, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6322331, Length=402, Percent_Identity=38.8059701492537, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6324878, Length=382, Percent_Identity=37.434554973822, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6322638, Length=181, Percent_Identity=30.3867403314917, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=40.6735751295337, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI45555749, Length=386, Percent_Identity=40.6735751295337, Blast_Score=259, Evalue=3e-69,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 44178; Mature: 44178

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSRRSNNEEQHDMTLLRKQKPALLVLEDGCVFRGEAFAGEGEVLGEVVFNTGMTGYQE
CCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHHCCCCHHHH
VLTDPSYKGQIVAMTYPLIGNYGINPEDMEAAGISLEGFIVREYSDFPSNWRSRQSLKTF
HHCCCCCCCEEEEEEECEECCCCCCCCCHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHH
MESQGKLGVEGLDCRALTRRLRVSGAMRGILTTEIQDVEILLQRVRAYPGLVGQDLVRTV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TCQEPYRWVRGSVQPADRDRRPEEGKNLRVVVLDCGVKYNILRCLEAEGCEVIVVPACSS
HCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCEEEEECCCC
DEQILSWSPDGLLLSNGPGDPAALPYIVATVRQLLGKVPIFGICLGHQILGQAVGGRTEK
CCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCE
LKFGHHGINQPVKHRLSGRVEITSQNHGFVVVPESLPADLEKTHDNLNDGTSEGIAYPSL
EECCCCCCCHHHHHHHCCEEEEEECCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCC
GAFSVQYHPEAAPGPQDASYLFKQFVKLITSEKGNKLRTPS
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRNSRRSNNEEQHDMTLLRKQKPALLVLEDGCVFRGEAFAGEGEVLGEVVFNTGMTGYQE
CCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHHCCCCHHHH
VLTDPSYKGQIVAMTYPLIGNYGINPEDMEAAGISLEGFIVREYSDFPSNWRSRQSLKTF
HHCCCCCCCEEEEEEECEECCCCCCCCCHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHH
MESQGKLGVEGLDCRALTRRLRVSGAMRGILTTEIQDVEILLQRVRAYPGLVGQDLVRTV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TCQEPYRWVRGSVQPADRDRRPEEGKNLRVVVLDCGVKYNILRCLEAEGCEVIVVPACSS
HCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCEEEEECCCC
DEQILSWSPDGLLLSNGPGDPAALPYIVATVRQLLGKVPIFGICLGHQILGQAVGGRTEK
CCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCE
LKFGHHGINQPVKHRLSGRVEITSQNHGFVVVPESLPADLEKTHDNLNDGTSEGIAYPSL
EECCCCCCCHHHHHHHCCEEEEEECCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCC
GAFSVQYHPEAAPGPQDASYLFKQFVKLITSEKGNKLRTPS
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA