Click here to switch to the map view.

The map label for this gene is glnA [H]

Identifier: 85860896

GI number: 85860896

Start: 3065799

End: 3067157

Strand: Direct

Name: glnA [H]

Synonym: SYN_01628

Alternate gene names: 85860896

Gene position: 3065799-3067157 (Clockwise)

Preceding gene: 85860895

Following gene: 85860897

Centisome position: 96.43

GC content: 54.89

Gene sequence:

>1359_bases
TTGATTCTTTGGAGAAAGGAGCCCTCTATGTTCAACTGTAAAACGAAAGAGGAAGTATTGCAGATCGTTAAAGAACAAAA
TGTCAGTTTCATTCAGTTCTGGTTTACCGATGTCCTCGGAGTGCAGAAAGTTTTCAGCGTCACCCCTTCGGAACTGAAGG
AAGGACTGGAGGAAGGAATGGGATTTGACGGATCCTCCATTCAGGGATTCTGCCGCATCGAGGAGAGCGACATGATTGCC
ATGCCCGATCCGACGACCTTTCAACTCATATCCTGGCGGCCGTCGGATCGACCGGTAGCGCGGATGATCTGCGATATCCT
GAAACCTGACGGGTCTCCCTATGAAGGGGATCCCCGCTATGTCCTCAAGCGGATGCTCAAGAAAATCGCGGATCAGGGCT
ACATCTTCTATGTCGGCCCGGAACTGGAGTTCTTTTATTTCACCAGCGACAGAGCCCCGGAATATCTCGACAGGGGTGGA
TATTTCGACGGCCTTCCCGTGGACCTGGCCACGGACCTGCGGCGGCAGACCATCTTCGCCCTCCAGGAGATGGGAATCCG
GGTCGAATATTCCCATCATGAGGTGGCGCCGAGCCAACACGAGATCGATCTTCGATATGACGAGGCCCTCTGCATGGCGG
ACAAGGTGATGACCTACAGGACAACGGTCAAGGAAATCGCCCGGCAGAACGGCGTCTACGCCTCTTTCATGCCCAAGCCG
ATCTTCGGGGAAAACGGCAGCGGGATGCATACCCATCAGTCCCTGTTTGTCGGGGACAAAAACGCTTTTTTCGACAGCGG
GGACAAGTATCAGCTCTCCGCTCTTGCGAAGCACTACATTGCCGGACTCATGAAGCATGCCCCGGAGATTACGGCGATCT
GCAATCAATGGGTAAATTCCTACAAACGGCTTGTGCCGGGCTACGAAGCGCCGGTTTATGTCTCCTGGGCGCGCCGCAAC
CGGTCCGCCATGATCCGCGTCCCCATGTACAAACCGGGCAAGGAAAATGCGACCCGGATCGAGTTCCGCTCCCCCGATCC
CGCCTGCAATCCCTATCTGGCCTTTGCCGTCATGCTGGCAGCCGGTCTGAAGGGCGTGGAGAAGGCCTATAAACTGCCGG
ATCCGGTGGAAGAGGATATCTTCGAAATGAATGAAAAAGCCCGCGAACAGGCCGGGATCACCTCCCTGCCGGGCAGTCTG
TATGAAGCGATCGTAGAGGTTTCCCAGAGTGAACTGGTCAGGGAGACCCTGGGGGATCATATCTTCGAGAAGTTCGTAGA
GAACAAAAAGGTGGAGTGGGACCGGTTCCGCACACATGTCAGCCGGTTTGAAATTGAACGCTATCTGCCGATGCTTTAA

Upstream 100 bases:

>100_bases
GAGCCGGGCGGAGCGGTGCATCGGATGCGGTTTCTGCGCTGGGGTCTGTCCGACAGGCGTCTGGCGACTGGTGGAAAACG
AACCCCTGGAATAACATGAT

Downstream 100 bases:

>100_bases
GTTTCCTCTCCTTCGACGAACGCGCCGTTTTTCTTTGGAGGAAACGAATCCGGTTATCAAAGGAAAATGATTTCAAAAAA
ATCGCTTCCCTTTGATAATT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase 2 [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MILWRKEPSMFNCKTKEEVLQIVKEQNVSFIQFWFTDVLGVQKVFSVTPSELKEGLEEGMGFDGSSIQGFCRIEESDMIA
MPDPTTFQLISWRPSDRPVARMICDILKPDGSPYEGDPRYVLKRMLKKIADQGYIFYVGPELEFFYFTSDRAPEYLDRGG
YFDGLPVDLATDLRRQTIFALQEMGIRVEYSHHEVAPSQHEIDLRYDEALCMADKVMTYRTTVKEIARQNGVYASFMPKP
IFGENGSGMHTHQSLFVGDKNAFFDSGDKYQLSALAKHYIAGLMKHAPEITAICNQWVNSYKRLVPGYEAPVYVSWARRN
RSAMIRVPMYKPGKENATRIEFRSPDPACNPYLAFAVMLAAGLKGVEKAYKLPDPVEEDIFEMNEKAREQAGITSLPGSL
YEAIVEVSQSELVRETLGDHIFEKFVENKKVEWDRFRTHVSRFEIERYLPML

Sequences:

>Translated_452_residues
MILWRKEPSMFNCKTKEEVLQIVKEQNVSFIQFWFTDVLGVQKVFSVTPSELKEGLEEGMGFDGSSIQGFCRIEESDMIA
MPDPTTFQLISWRPSDRPVARMICDILKPDGSPYEGDPRYVLKRMLKKIADQGYIFYVGPELEFFYFTSDRAPEYLDRGG
YFDGLPVDLATDLRRQTIFALQEMGIRVEYSHHEVAPSQHEIDLRYDEALCMADKVMTYRTTVKEIARQNGVYASFMPKP
IFGENGSGMHTHQSLFVGDKNAFFDSGDKYQLSALAKHYIAGLMKHAPEITAICNQWVNSYKRLVPGYEAPVYVSWARRN
RSAMIRVPMYKPGKENATRIEFRSPDPACNPYLAFAVMLAAGLKGVEKAYKLPDPVEEDIFEMNEKAREQAGITSLPGSL
YEAIVEVSQSELVRETLGDHIFEKFVENKKVEWDRFRTHVSRFEIERYLPML
>Mature_452_residues
MILWRKEPSMFNCKTKEEVLQIVKEQNVSFIQFWFTDVLGVQKVFSVTPSELKEGLEEGMGFDGSSIQGFCRIEESDMIA
MPDPTTFQLISWRPSDRPVARMICDILKPDGSPYEGDPRYVLKRMLKKIADQGYIFYVGPELEFFYFTSDRAPEYLDRGG
YFDGLPVDLATDLRRQTIFALQEMGIRVEYSHHEVAPSQHEIDLRYDEALCMADKVMTYRTTVKEIARQNGVYASFMPKP
IFGENGSGMHTHQSLFVGDKNAFFDSGDKYQLSALAKHYIAGLMKHAPEITAICNQWVNSYKRLVPGYEAPVYVSWARRN
RSAMIRVPMYKPGKENATRIEFRSPDPACNPYLAFAVMLAAGLKGVEKAYKLPDPVEEDIFEMNEKAREQAGITSLPGSL
YEAIVEVSQSELVRETLGDHIFEKFVENKKVEWDRFRTHVSRFEIERYLPML

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=441, Percent_Identity=27.2108843537415, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI74271826, Length=281, Percent_Identity=25.9786476868327, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI74271837, Length=281, Percent_Identity=25.9786476868327, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI19923206, Length=281, Percent_Identity=25.9786476868327, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790301, Length=477, Percent_Identity=37.7358490566038, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI87081870, Length=451, Percent_Identity=26.8292682926829, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51957; Mature: 51957

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILWRKEPSMFNCKTKEEVLQIVKEQNVSFIQFWFTDVLGVQKVFSVTPSELKEGLEEGM
CEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
GFDGSSIQGFCRIEESDMIAMPDPTTFQLISWRPSDRPVARMICDILKPDGSPYEGDPRY
CCCCCCCCEEEEECCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHH
VLKRMLKKIADQGYIFYVGPELEFFYFTSDRAPEYLDRGGYFDGLPVDLATDLRRQTIFA
HHHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LQEMGIRVEYSHHEVAPSQHEIDLRYDEALCMADKVMTYRTTVKEIARQNGVYASFMPKP
HHHCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
IFGENGSGMHTHQSLFVGDKNAFFDSGDKYQLSALAKHYIAGLMKHAPEITAICNQWVNS
CCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YKRLVPGYEAPVYVSWARRNRSAMIRVPMYKPGKENATRIEFRSPDPACNPYLAFAVMLA
HHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHH
AGLKGVEKAYKLPDPVEEDIFEMNEKAREQAGITSLPGSLYEAIVEVSQSELVRETLGDH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IFEKFVENKKVEWDRFRTHVSRFEIERYLPML
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILWRKEPSMFNCKTKEEVLQIVKEQNVSFIQFWFTDVLGVQKVFSVTPSELKEGLEEGM
CEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
GFDGSSIQGFCRIEESDMIAMPDPTTFQLISWRPSDRPVARMICDILKPDGSPYEGDPRY
CCCCCCCCEEEEECCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHH
VLKRMLKKIADQGYIFYVGPELEFFYFTSDRAPEYLDRGGYFDGLPVDLATDLRRQTIFA
HHHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LQEMGIRVEYSHHEVAPSQHEIDLRYDEALCMADKVMTYRTTVKEIARQNGVYASFMPKP
HHHCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
IFGENGSGMHTHQSLFVGDKNAFFDSGDKYQLSALAKHYIAGLMKHAPEITAICNQWVNS
CCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YKRLVPGYEAPVYVSWARRNRSAMIRVPMYKPGKENATRIEFRSPDPACNPYLAFAVMLA
HHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHH
AGLKGVEKAYKLPDPVEEDIFEMNEKAREQAGITSLPGSLYEAIVEVSQSELVRETLGDH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IFEKFVENKKVEWDRFRTHVSRFEIERYLPML
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA