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The map label for this gene is afsQ1 [H]

Identifier: 85860599

GI number: 85860599

Start: 2772045

End: 2772446

Strand: Direct

Name: afsQ1 [H]

Synonym: SYN_01292

Alternate gene names: 85860599

Gene position: 2772045-2772446 (Clockwise)

Preceding gene: 85860598

Following gene: 85860602

Centisome position: 87.19

GC content: 50.75

Gene sequence:

>402_bases
GTGGGCGATAAAAAAATTCTTCTTGTAGATGATGAAGATCAAATCCTTCAGATGCTGAAAGAGGCGTTCAGCCTTTACGG
CTATAAGGTCAAGACTGCATCAAGCGCGGAGGAGGCCCTGGACGTTCTCGCGAAGGAAAGCATCATGGTCATGTTCCTGG
ATCTCAAACTCCCCGGCATGAGTGGCCTCGATCTTTGCCGGCAGATCAGAAAAGACAATCAGATCGCCGTTATCCACGCT
CTGACCGGCTACAGCAACTTTTTCGGGTTTCTGGAATGCCGGAGTGCCGGTTTTGACGATTTCTTCACGAAACCGGTCGC
GCTGAAGGCGCTTTTCAAGGCTGCCGAGGAGGCCTTTGAAAAACTCGAGCGCTGGAAAGTTTTTGTATACGACCTGGCAT
GA

Upstream 100 bases:

>100_bases
CCCTTCTGTTCAGGATTACGCCGGGATAGGCAAGATTGTGAATCTGAATGGAGACCAGGGGAAATAACGGAATCTATTTT
TCAGAAAGGAAAATTTTACT

Downstream 100 bases:

>100_bases
CCTCATCCACGCGCCCCCATCACGCCCCGCGTGACGAGACAAAGTCCCGTTCCGCCCGCCGCCTGTTAAACCGAACTCCT
GATCCGTTCAGAGAATCACG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MGDKKILLVDDEDQILQMLKEAFSLYGYKVKTASSAEEALDVLAKESIMVMFLDLKLPGMSGLDLCRQIRKDNQIAVIHA
LTGYSNFFGFLECRSAGFDDFFTKPVALKALFKAAEEAFEKLERWKVFVYDLA

Sequences:

>Translated_133_residues
MGDKKILLVDDEDQILQMLKEAFSLYGYKVKTASSAEEALDVLAKESIMVMFLDLKLPGMSGLDLCRQIRKDNQIAVIHA
LTGYSNFFGFLECRSAGFDDFFTKPVALKALFKAAEEAFEKLERWKVFVYDLA
>Mature_132_residues
GDKKILLVDDEDQILQMLKEAFSLYGYKVKTASSAEEALDVLAKESIMVMFLDLKLPGMSGLDLCRQIRKDNQIAVIHAL
TGYSNFFGFLECRSAGFDDFFTKPVALKALFKAAEEAFEKLERWKVFVYDLA

Specific function: Forms part of a two-component regulatory system AfsQ1/AfsQ2 involved in secondary metabolism [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=108, Percent_Identity=31.4814814814815, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=116, Percent_Identity=28.448275862069, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1788905, Length=126, Percent_Identity=32.5396825396825, Blast_Score=60, Evalue=5e-11,
Organism=Escherichia coli, GI1790863, Length=111, Percent_Identity=33.3333333333333, Blast_Score=59, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 15075; Mature: 14944

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDKKILLVDDEDQILQMLKEAFSLYGYKVKTASSAEEALDVLAKESIMVMFLDLKLPGM
CCCCEEEEECCHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCC
SGLDLCRQIRKDNQIAVIHALTGYSNFFGFLECRSAGFDDFFTKPVALKALFKAAEEAFE
CHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHH
KLERWKVFVYDLA
HHHHHEEEEEECC
>Mature Secondary Structure 
GDKKILLVDDEDQILQMLKEAFSLYGYKVKTASSAEEALDVLAKESIMVMFLDLKLPGM
CCCEEEEECCHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCC
SGLDLCRQIRKDNQIAVIHALTGYSNFFGFLECRSAGFDDFFTKPVALKALFKAAEEAFE
CHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHH
KLERWKVFVYDLA
HHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339426; 12000953 [H]