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The map label for this gene is nth [H]

Identifier: 85860567

GI number: 85860567

Start: 2743110

End: 2743730

Strand: Direct

Name: nth [H]

Synonym: SYN_00287

Alternate gene names: 85860567

Gene position: 2743110-2743730 (Clockwise)

Preceding gene: 85860566

Following gene: 85860569

Centisome position: 86.28

GC content: 56.84

Gene sequence:

>621_bases
ATGGAAAAAGAACGGATCGCCCTTCTGATTCAAAAGCTTGAAGACAGCGCGGGAGCCGCGGAGTTCCCCATCGTCACCCA
GCTGGCGGAAACCCGCCGCGATCCTTTTCTGATTCTGATTTCCACAGTACTCAGTCTCCGGACAAAGGATGAAGTCACGG
CCACCGCTACGGAGCGGCTCTTCAGCCTGGCCTCAACACCGGAAACCATGCTGGAGCTGAGTGAAGAGGAAATACGCCAG
GCCATCTATCCCGTCGGCTTTTACCGAAACAAATCCCGGCAGATTCGCGAAATCTGCCGGGATCTTATTGAGCGATTTTC
CTCCCGTGTTCCCGATTCCCTGGAAGACCTGCTTTCCCTCAAAGGCGTTGGACAGAAAACAGCCAATCTGGTCCTTTCCC
TCGGCTTCGAGAAAGACGCCATCTGTGTGGACACCCATGTACACCGGATTTCCAACCGCCTCGGCCTGGTGAGCACGAAA
ACGCCGGAACAGACGGAATCGGCCTTACAGAACGTCCTGCCGCGCCGTTACTGGTCCCGGTACAACACCCTCCTGGTCTC
CTTCGGTCAGCGCGTCTGCCGCCCTCTTTCTCCCCTCTGCAGTTCCTGTCCCCTTTGCTGA

Upstream 100 bases:

>100_bases
GGAAGCCAATCTCGACGTGGAAGACATTGCTACCCTTCATCTGGACATGCTGGCCTACGAAGAAGGATACAGTTAGCCTG
AAGAGCTGCCGGCCTGGCTC

Downstream 100 bases:

>100_bases
ACTCTGTCCACGCATCAACGTCACCCGGCATCGATGAATTCTGCCCGGTCGCCTCTGTTTCTCTCGCTTCGCGCTTCTTT
CGGCGGTTCCCGGCACATGA

Product: endonuclease III N

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MEKERIALLIQKLEDSAGAAEFPIVTQLAETRRDPFLILISTVLSLRTKDEVTATATERLFSLASTPETMLELSEEEIRQ
AIYPVGFYRNKSRQIREICRDLIERFSSRVPDSLEDLLSLKGVGQKTANLVLSLGFEKDAICVDTHVHRISNRLGLVSTK
TPEQTESALQNVLPRRYWSRYNTLLVSFGQRVCRPLSPLCSSCPLC

Sequences:

>Translated_206_residues
MEKERIALLIQKLEDSAGAAEFPIVTQLAETRRDPFLILISTVLSLRTKDEVTATATERLFSLASTPETMLELSEEEIRQ
AIYPVGFYRNKSRQIREICRDLIERFSSRVPDSLEDLLSLKGVGQKTANLVLSLGFEKDAICVDTHVHRISNRLGLVSTK
TPEQTESALQNVLPRRYWSRYNTLLVSFGQRVCRPLSPLCSSCPLC
>Mature_206_residues
MEKERIALLIQKLEDSAGAAEFPIVTQLAETRRDPFLILISTVLSLRTKDEVTATATERLFSLASTPETMLELSEEEIRQ
AIYPVGFYRNKSRQIREICRDLIERFSSRVPDSLEDLLSLKGVGQKTANLVLSLGFEKDAICVDTHVHRISNRLGLVSTK
TPEQTESALQNVLPRRYWSRYNTLLVSFGQRVCRPLSPLCSSCPLC

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=171, Percent_Identity=40.9356725146199, Blast_Score=145, Evalue=2e-35,
Organism=Escherichia coli, GI1787920, Length=169, Percent_Identity=37.2781065088757, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1789331, Length=211, Percent_Identity=22.7488151658768, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=172, Percent_Identity=44.1860465116279, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319304, Length=170, Percent_Identity=38.2352941176471, Blast_Score=114, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6324530, Length=223, Percent_Identity=31.8385650224215, Blast_Score=99, Evalue=4e-22,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=39.8876404494382, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23286; Mature: 23286

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKERIALLIQKLEDSAGAAEFPIVTQLAETRRDPFLILISTVLSLRTKDEVTATATERL
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FSLASTPETMLELSEEEIRQAIYPVGFYRNKSRQIREICRDLIERFSSRVPDSLEDLLSL
HHHHCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KGVGQKTANLVLSLGFEKDAICVDTHVHRISNRLGLVSTKTPEQTESALQNVLPRRYWSR
HCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
YNTLLVSFGQRVCRPLSPLCSSCPLC
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEKERIALLIQKLEDSAGAAEFPIVTQLAETRRDPFLILISTVLSLRTKDEVTATATERL
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FSLASTPETMLELSEEEIRQAIYPVGFYRNKSRQIREICRDLIERFSSRVPDSLEDLLSL
HHHHCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KGVGQKTANLVLSLGFEKDAICVDTHVHRISNRLGLVSTKTPEQTESALQNVLPRRYWSR
HCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
YNTLLVSFGQRVCRPLSPLCSSCPLC
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]