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The map label for this gene is thiM

Identifier: 85860552

GI number: 85860552

Start: 2727792

End: 2728604

Strand: Direct

Name: thiM

Synonym: SYN_00303

Alternate gene names: 85860552

Gene position: 2727792-2728604 (Clockwise)

Preceding gene: 85860550

Following gene: 85860553

Centisome position: 85.8

GC content: 61.99

Gene sequence:

>813_bases
ATGCAGATCACCCCTGAAAACACTTGGGCCGCGGTGAAGGCGATTCGCAGCAGAGCGCCCCTGATCCACAACATCACCAA
TTATGTCGTGATGAACAGCACCGCCAACGCCCTGCTGGCTCTTGGCGCGTCACCCGTCATGGCCCACGCGCAGGAAGAGG
TGGAAGAAATGGTCGGCATCGCCCAGGCTCTCGTGGTCAATATCGGAACCCTCAGCCCGTCCTGGGTCCGGGCCATGGCC
AGCGCCGCGCTGAAAGCGGCCGACCGCGGAATTCCCATCATTCTGGACCCGGTCGGCGCCGGCGCCACAGCCTACCGGAC
CCGCACGGCCTTTCAACTCCTGGAAACGGTTTCCCCGACAATCATCCGGGGCAACGCATCGGAAATCCTGGCGTTTGAGA
GTGTCGAAACAATGGAAACCAGGGGAGTGGACAGCACGGAACTCTCACAGAACGCCGTCGACGCCGGACGTCGGCTGAAC
GATTCCTACGGCAGTACCGTCTGTATCAGCGGGGAAACAGATTTCATCATCGGAGACCGTGCCATCCTCGGGATCAGGAA
CGGCCACCCCCTGATGACCCGTGTGACAGGCCTCGGCTGCACGGCCTCCGCACTGTGCGGCGCCTTCGCCGCCGTTAATT
CCTCCTTTACACTGGCCGCCGCGCAGGCCATGGCGGTCATGGGCATCGCGGGTGAAATGGCAGCGGAAATCTCTCCCGGG
CCGGGCAGTCTTCAGCTTCACTTCCTCGACATCCTTTACCGGCTTACGGAAGAGGACATTGCACGCCGTCTGCGGATCGT
AGCGGGAGAATGA

Upstream 100 bases:

>100_bases
ATTGTTCCCTTATGATGTCAAGCGACAATTCCCCCTTGCAAGAGCCTGACCTGAAGGATAAGATACCCCCCTGTTTCAAA
CCCTCAAGAGGAGTACAGCC

Downstream 100 bases:

>100_bases
CCATGATCCAGCCCAGGGCTCATTCCCGGAAAAAAATTGCCGGTCTGTATCTGGTCACGGACCGCGCTCTCTGCGGTAAC
AGGGGTCTCGAAGAGGTCGT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MQITPENTWAAVKAIRSRAPLIHNITNYVVMNSTANALLALGASPVMAHAQEEVEEMVGIAQALVVNIGTLSPSWVRAMA
SAALKAADRGIPIILDPVGAGATAYRTRTAFQLLETVSPTIIRGNASEILAFESVETMETRGVDSTELSQNAVDAGRRLN
DSYGSTVCISGETDFIIGDRAILGIRNGHPLMTRVTGLGCTASALCGAFAAVNSSFTLAAAQAMAVMGIAGEMAAEISPG
PGSLQLHFLDILYRLTEEDIARRLRIVAGE

Sequences:

>Translated_270_residues
MQITPENTWAAVKAIRSRAPLIHNITNYVVMNSTANALLALGASPVMAHAQEEVEEMVGIAQALVVNIGTLSPSWVRAMA
SAALKAADRGIPIILDPVGAGATAYRTRTAFQLLETVSPTIIRGNASEILAFESVETMETRGVDSTELSQNAVDAGRRLN
DSYGSTVCISGETDFIIGDRAILGIRNGHPLMTRVTGLGCTASALCGAFAAVNSSFTLAAAQAMAVMGIAGEMAAEISPG
PGSLQLHFLDILYRLTEEDIARRLRIVAGE
>Mature_270_residues
MQITPENTWAAVKAIRSRAPLIHNITNYVVMNSTANALLALGASPVMAHAQEEVEEMVGIAQALVVNIGTLSPSWVRAMA
SAALKAADRGIPIILDPVGAGATAYRTRTAFQLLETVSPTIIRGNASEILAFESVETMETRGVDSTELSQNAVDAGRRLN
DSYGSTVCISGETDFIIGDRAILGIRNGHPLMTRVTGLGCTASALCGAFAAVNSSFTLAAAQAMAVMGIAGEMAAEISPG
PGSLQLHFLDILYRLTEEDIARRLRIVAGE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=248, Percent_Identity=42.3387096774194, Blast_Score=171, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6325042, Length=279, Percent_Identity=29.3906810035842, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_SYNAS (Q2LWX9)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_462754.1
- ProteinModelPortal:   Q2LWX9
- SMR:   Q2LWX9
- STRING:   Q2LWX9
- GeneID:   3883369
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_00303
- NMPDR:   fig|56780.10.peg.2695
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- PhylomeDB:   Q2LWX9
- ProtClustDB:   PRK09355
- BioCyc:   SACI56780:SYN_00303-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28368; Mature: 28368

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: BINDING 47-47 BINDING 123-123 BINDING 170-170 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITPENTWAAVKAIRSRAPLIHNITNYVVMNSTANALLALGASPVMAHAQEEVEEMVGI
CCCCCCHHHHHHHHHHHCCCHHHHCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHH
AQALVVNIGTLSPSWVRAMASAALKAADRGIPIILDPVGAGATAYRTRTAFQLLETVSPT
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCE
IIRGNASEILAFESVETMETRGVDSTELSQNAVDAGRRLNDSYGSTVCISGETDFIIGDR
EEECCHHHEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECE
AILGIRNGHPLMTRVTGLGCTASALCGAFAAVNSSFTLAAAQAMAVMGIAGEMAAEISPG
EEEEECCCCCHHHHEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PGSLQLHFLDILYRLTEEDIARRLRIVAGE
CCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQITPENTWAAVKAIRSRAPLIHNITNYVVMNSTANALLALGASPVMAHAQEEVEEMVGI
CCCCCCHHHHHHHHHHHCCCHHHHCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHH
AQALVVNIGTLSPSWVRAMASAALKAADRGIPIILDPVGAGATAYRTRTAFQLLETVSPT
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCE
IIRGNASEILAFESVETMETRGVDSTELSQNAVDAGRRLNDSYGSTVCISGETDFIIGDR
EEECCHHHEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECE
AILGIRNGHPLMTRVTGLGCTASALCGAFAAVNSSFTLAAAQAMAVMGIAGEMAAEISPG
EEEEECCCCCHHHHEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PGSLQLHFLDILYRLTEEDIARRLRIVAGE
CCCEEEHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA