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The map label for this gene is braE [H]

Identifier: 85860151

GI number: 85860151

Start: 2357057

End: 2358259

Strand: Direct

Name: braE [H]

Synonym: SYN_00787

Alternate gene names: 85860151

Gene position: 2357057-2358259 (Clockwise)

Preceding gene: 85860150

Following gene: 85860152

Centisome position: 74.14

GC content: 53.7

Gene sequence:

>1203_bases
ATGAAGAAGGTAAAGAATGCCGTCCTGACCTCCCTCTGGTTCATGTTTCTCACGTTTCCTCTACTGGCGATCCGGGTGAA
CACGATCGAAAACATTGTGGAATGGCGTTGGAAAAACATGATTATCGTCGGAATCGGCACCTTCGTTATGTCGCTTCTCT
GGCAATACATGCAGGCCAGAAAGGGATCAGGTCCGGGACCGGCTGAAAAGAAAAATGAATACCTGCATTTTTTTTCCCGA
AAAATTCTCGATGAACCGTTGATTTACAAGTCGATCCTTGTTTCCGGTGGACTGTTCGCCCTTGTTTTTCCCTTTCTTTT
CTCCAGTTATCAGACCAGCATCATGACGACGGCTCTTCTTTACATCGTTCTCGGACTGGGACTGAACATCGTCGTGGGAC
TTGCGGGCCTGCTCGATCTAGGCTATGTGGCCTTTTATGCCGTAGGCGCTTACACCTATGCCCTTCTCAATGTCCACTTC
GGCGTGGGATTCTGGACCGCGCTGCCCATCGGCGCCCTGCTCGCCGCCTGTTTCGGCGTTATCCTCGGCTTTCCCGTCCT
GAGGCTCCGGGGGGATTATCTGGCAATCGTAACCCTGGGATTCGGAGAAATCATCCGTCTCATTCTCGAAAACTGGAATG
CCTTTTCTTTTGGCCCCAGCGGTATTTCCAACATTCCTCGCCCTGGCTTCTTCGGCATCGAACTCAGCCTGCAAAACGCC
ACAATTTATATCTATTATCTCATGATCGCCCTCTGCCTCTTCACCATTCTGGTGGTTAACCGCCTCCAGCATTCGCGTAT
CGGCCGGGCCTGGATCGCTCTTCGGGAGGATGAACTGGCCTGCGAAGCCATGGGGGTTGACAAAACTCGAACGAAACTGA
CCGCTTTTGCCCTGGGAGCCACCTGGGCCGGCATGGCCGGGGTGATCCTGGCCGCCAAGACCACCTTCATCAATCCGGCG
AGCTTCACCTTTCTGGAATCGGCCATGATTCTATCCATCGTTGTGCTGGGAGGCATGGGTTCAATCCCCGGCGTGATTCT
GGGCGCCCTGCTGTTGATGCTTCTTCCGGAATACCTGAGGGTCTTCAAGGATTACAGGATGCTCCTCTTCGGAGCCACCA
TGGTGCTGATGATGGTCTTCCGGCCACAGGGCATGATCACGGCCGTCCGGCGGACCTATCAGTACAAGGAGAACGATTCG
TGA

Upstream 100 bases:

>100_bases
CGATTACGAAGATGTCTTTGCGTTCCTGCTGCTGGTGCTCGTTTTGATTTTCCGACCCGCCGGCCTTCTGGGGCGATCTC
CAACCCGCAAGGTATAAGTC

Downstream 100 bases:

>100_bases
CTTCCGAAACGCCCTATCCCGCCCTGGAGGTTCTTGACCTGACGATGGATTTCGGCGGTCTGCGCGCCCTCAACGCTGCC
ACACTTCATGTCAAGGGGGG

Product: branched-chain amino acid transport system permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKKVKNAVLTSLWFMFLTFPLLAIRVNTIENIVEWRWKNMIIVGIGTFVMSLLWQYMQARKGSGPGPAEKKNEYLHFFSR
KILDEPLIYKSILVSGGLFALVFPFLFSSYQTSIMTTALLYIVLGLGLNIVVGLAGLLDLGYVAFYAVGAYTYALLNVHF
GVGFWTALPIGALLAACFGVILGFPVLRLRGDYLAIVTLGFGEIIRLILENWNAFSFGPSGISNIPRPGFFGIELSLQNA
TIYIYYLMIALCLFTILVVNRLQHSRIGRAWIALREDELACEAMGVDKTRTKLTAFALGATWAGMAGVILAAKTTFINPA
SFTFLESAMILSIVVLGGMGSIPGVILGALLLMLLPEYLRVFKDYRMLLFGATMVLMMVFRPQGMITAVRRTYQYKENDS

Sequences:

>Translated_400_residues
MKKVKNAVLTSLWFMFLTFPLLAIRVNTIENIVEWRWKNMIIVGIGTFVMSLLWQYMQARKGSGPGPAEKKNEYLHFFSR
KILDEPLIYKSILVSGGLFALVFPFLFSSYQTSIMTTALLYIVLGLGLNIVVGLAGLLDLGYVAFYAVGAYTYALLNVHF
GVGFWTALPIGALLAACFGVILGFPVLRLRGDYLAIVTLGFGEIIRLILENWNAFSFGPSGISNIPRPGFFGIELSLQNA
TIYIYYLMIALCLFTILVVNRLQHSRIGRAWIALREDELACEAMGVDKTRTKLTAFALGATWAGMAGVILAAKTTFINPA
SFTFLESAMILSIVVLGGMGSIPGVILGALLLMLLPEYLRVFKDYRMLLFGATMVLMMVFRPQGMITAVRRTYQYKENDS
>Mature_400_residues
MKKVKNAVLTSLWFMFLTFPLLAIRVNTIENIVEWRWKNMIIVGIGTFVMSLLWQYMQARKGSGPGPAEKKNEYLHFFSR
KILDEPLIYKSILVSGGLFALVFPFLFSSYQTSIMTTALLYIVLGLGLNIVVGLAGLLDLGYVAFYAVGAYTYALLNVHF
GVGFWTALPIGALLAACFGVILGFPVLRLRGDYLAIVTLGFGEIIRLILENWNAFSFGPSGISNIPRPGFFGIELSLQNA
TIYIYYLMIALCLFTILVVNRLQHSRIGRAWIALREDELACEAMGVDKTRTKLTAFALGATWAGMAGVILAAKTTFINPA
SFTFLESAMILSIVVLGGMGSIPGVILGALLLMLLPEYLRVFKDYRMLLFGATMVLMMVFRPQGMITAVRRTYQYKENDS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=315, Percent_Identity=50.7936507936508, Blast_Score=296, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 44417; Mature: 44417

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKNAVLTSLWFMFLTFPLLAIRVNTIENIVEWRWKNMIIVGIGTFVMSLLWQYMQAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHH
KGSGPGPAEKKNEYLHFFSRKILDEPLIYKSILVSGGLFALVFPFLFSSYQTSIMTTALL
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
YIVLGLGLNIVVGLAGLLDLGYVAFYAVGAYTYALLNVHFGVGFWTALPIGALLAACFGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGFPVLRLRGDYLAIVTLGFGEIIRLILENWNAFSFGPSGISNIPRPGFFGIELSLQNA
HHCCHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
TIYIYYLMIALCLFTILVVNRLQHSRIGRAWIALREDELACEAMGVDKTRTKLTAFALGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHCCCCHHHHHHHHHHHHH
TWAGMAGVILAAKTTFINPASFTFLESAMILSIVVLGGMGSIPGVILGALLLMLLPEYLR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VFKDYRMLLFGATMVLMMVFRPQGMITAVRRTYQYKENDS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKVKNAVLTSLWFMFLTFPLLAIRVNTIENIVEWRWKNMIIVGIGTFVMSLLWQYMQAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHH
KGSGPGPAEKKNEYLHFFSRKILDEPLIYKSILVSGGLFALVFPFLFSSYQTSIMTTALL
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
YIVLGLGLNIVVGLAGLLDLGYVAFYAVGAYTYALLNVHFGVGFWTALPIGALLAACFGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGFPVLRLRGDYLAIVTLGFGEIIRLILENWNAFSFGPSGISNIPRPGFFGIELSLQNA
HHCCHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
TIYIYYLMIALCLFTILVVNRLQHSRIGRAWIALREDELACEAMGVDKTRTKLTAFALGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHCCCCHHHHHHHHHHHHH
TWAGMAGVILAAKTTFINPASFTFLESAMILSIVVLGGMGSIPGVILGALLLMLLPEYLR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VFKDYRMLLFGATMVLMMVFRPQGMITAVRRTYQYKENDS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]