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The map label for this gene is hemN [C]

Identifier: 85860142

GI number: 85860142

Start: 2348746

End: 2349837

Strand: Direct

Name: hemN [C]

Synonym: SYN_00795

Alternate gene names: 85860142

Gene position: 2348746-2349837 (Clockwise)

Preceding gene: 85860141

Following gene: 85860143

Centisome position: 73.88

GC content: 58.06

Gene sequence:

>1092_bases
ATGAAGGAGAGTGAATTTTCCGTGGCATCCCCCCTGATTATCCCCGTTTTTCTGCCCTGGCAGGGCTGTCGGCAACGCTG
CATCTTCTGCAACGAAAGCATTACCACGGGAACATTCCCGGCTGCGTTCAGCGAGGCTGCTTTCGAAAAAACCGTGACAC
TTTATTCGGAAAATCAGAAACGGGGGAAAAGCCCGGTTCAGATCGCATTTTACGGCGGGACCTTTACCGGCATGGACCGG
AAGGAACAGACCAGGCTCCTGCAGTTGGCCGAGCCATACATCAGAGAAGGACGAGTCGACAGCCTCCGGATTTCCACAAG
GCCGGATGAGATTGACGAAGACCTTCTGGATTTCCTGCGGCGCCACGGGGTCAGGACTGTGGAACTGGGTGCTCAGTCGC
TTGTGGAGGAAGTCCTGCGGCATTCCCGGCGGGGCCATACCGTGGGAGATGTGTTCTCCGCGGTGGAACGGCTGCGGAGA
AAAGGTTTTGAAGTCGGGATCCATCTGATGATGGGACTTCCCGGCGACAGTCCACAATTTTTTGCCGCCACCATCGACCG
GACTCTCGATCTTCAGCCCGACACGGTCAGGCTTCACCCGACCCTGGTCTTCCGGAACACGGTCCTCGCGACCCTTTACG
CCAGGGGACAATATTCCCCCCTCTCGCTGAATGAAGCCGTGGGACTGTCACGGTACGCCCTGCTCCGTCTGGAAAAGGGG
GGAATCCCCGTCATCAGACTCGGCCTTCAGAGCACCCCGGAAATGGAACTGCCGGGAAATATCGTCGCCGGCCCTTATCA
TCCGGCCTTCCGCTCACTGGTCAACGCCGCCATCTTTCGGGACATGGCCACAACCCTGCTCTCCGAAGCAAGGTCTGAAG
GGCGGGCGGTTTCTTTTTTCCTGTCACCGCGGGATGTGTCGGACTTCCGTGGGCATAAAGGGGAAAACCTCTCCTTACTT
GCAGAACGCTTCCGCCCCCTGAAGATCGAGGTCACCAACGATCCGGATTATACACGGGGATGGCTGACACTACGGATCGG
AGACCGCAGCATCTCCATGCATCGCACCGGTCTCCGCCCCATCCTTTCATGA

Upstream 100 bases:

>100_bases
ATTCCCGGGATCATCGAAACAACCGGAAAAGGAAAGAGCAAAAAAGAAGCGGAACAGCACGCGGCACACAAAGCGCTGGA
CGAACTGCTGAAGAATACCC

Downstream 100 bases:

>100_bases
ATGGTCCGGCCGTTTCTGGTAAACCTGCGAGTGGGCACGGTGGCGCTCCCAATTGTCCAGATACTGACTGGCGATCCCCG
GCTCCCGGATAATAAGCAGG

Product: radical SAM protein

Products: Protoporphyrinogen-IX; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKESEFSVASPLIIPVFLPWQGCRQRCIFCNESITTGTFPAAFSEAAFEKTVTLYSENQKRGKSPVQIAFYGGTFTGMDR
KEQTRLLQLAEPYIREGRVDSLRISTRPDEIDEDLLDFLRRHGVRTVELGAQSLVEEVLRHSRRGHTVGDVFSAVERLRR
KGFEVGIHLMMGLPGDSPQFFAATIDRTLDLQPDTVRLHPTLVFRNTVLATLYARGQYSPLSLNEAVGLSRYALLRLEKG
GIPVIRLGLQSTPEMELPGNIVAGPYHPAFRSLVNAAIFRDMATTLLSEARSEGRAVSFFLSPRDVSDFRGHKGENLSLL
AERFRPLKIEVTNDPDYTRGWLTLRIGDRSISMHRTGLRPILS

Sequences:

>Translated_363_residues
MKESEFSVASPLIIPVFLPWQGCRQRCIFCNESITTGTFPAAFSEAAFEKTVTLYSENQKRGKSPVQIAFYGGTFTGMDR
KEQTRLLQLAEPYIREGRVDSLRISTRPDEIDEDLLDFLRRHGVRTVELGAQSLVEEVLRHSRRGHTVGDVFSAVERLRR
KGFEVGIHLMMGLPGDSPQFFAATIDRTLDLQPDTVRLHPTLVFRNTVLATLYARGQYSPLSLNEAVGLSRYALLRLEKG
GIPVIRLGLQSTPEMELPGNIVAGPYHPAFRSLVNAAIFRDMATTLLSEARSEGRAVSFFLSPRDVSDFRGHKGENLSLL
AERFRPLKIEVTNDPDYTRGWLTLRIGDRSISMHRTGLRPILS
>Mature_363_residues
MKESEFSVASPLIIPVFLPWQGCRQRCIFCNESITTGTFPAAFSEAAFEKTVTLYSENQKRGKSPVQIAFYGGTFTGMDR
KEQTRLLQLAEPYIREGRVDSLRISTRPDEIDEDLLDFLRRHGVRTVELGAQSLVEEVLRHSRRGHTVGDVFSAVERLRR
KGFEVGIHLMMGLPGDSPQFFAATIDRTLDLQPDTVRLHPTLVFRNTVLATLYARGQYSPLSLNEAVGLSRYALLRLEKG
GIPVIRLGLQSTPEMELPGNIVAGPYHPAFRSLVNAAIFRDMATTLLSEARSEGRAVSFFLSPRDVSDFRGHKGENLSLL
AERFRPLKIEVTNDPDYTRGWLTLRIGDRSISMHRTGLRPILS

Specific function: Anaerobic Transformation Of Coproporphyrinogen-III Into Protoporphyrinogen-IX. [C]

COG id: COG1243

COG function: function code KB; Histone acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI23510283, Length=133, Percent_Identity=34.5864661654135, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI87082341, Length=211, Percent_Identity=28.9099526066351, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI2367204, Length=212, Percent_Identity=24.0566037735849, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133955098, Length=122, Percent_Identity=35.2459016393443, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325171, Length=133, Percent_Identity=35.3383458646617, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR006638
- InterPro:   IPR005910
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF04055 Radical_SAM [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40792; Mature: 40792

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESEFSVASPLIIPVFLPWQGCRQRCIFCNESITTGTFPAAFSEAAFEKTVTLYSENQK
CCCCCCCCCCCEEEEEECCCHHHHCCEEEECCCCCCCCCCHHHHHHHHHHEEEEEECCHH
RGKSPVQIAFYGGTFTGMDRKEQTRLLQLAEPYIREGRVDSLRISTRPDEIDEDLLDFLR
CCCCCEEEEEECCEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
RHGVRTVELGAQSLVEEVLRHSRRGHTVGDVFSAVERLRRKGFEVGIHLMMGLPGDSPQF
HCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCE
FAATIDRTLDLQPDTVRLHPTLVFRNTVLATLYARGQYSPLSLNEAVGLSRYALLRLEKG
EEEECCCEECCCCCEEEEEEEEEEHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEECCC
GIPVIRLGLQSTPEMELPGNIVAGPYHPAFRSLVNAAIFRDMATTLLSEARSEGRAVSFF
CCCEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LSPRDVSDFRGHKGENLSLLAERFRPLKIEVTNDPDYTRGWLTLRIGDRSISMHRTGLRP
ECCCCCHHHCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCEEEEHHCCCCC
ILS
CCC
>Mature Secondary Structure
MKESEFSVASPLIIPVFLPWQGCRQRCIFCNESITTGTFPAAFSEAAFEKTVTLYSENQK
CCCCCCCCCCCEEEEEECCCHHHHCCEEEECCCCCCCCCCHHHHHHHHHHEEEEEECCHH
RGKSPVQIAFYGGTFTGMDRKEQTRLLQLAEPYIREGRVDSLRISTRPDEIDEDLLDFLR
CCCCCEEEEEECCEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
RHGVRTVELGAQSLVEEVLRHSRRGHTVGDVFSAVERLRRKGFEVGIHLMMGLPGDSPQF
HCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCE
FAATIDRTLDLQPDTVRLHPTLVFRNTVLATLYARGQYSPLSLNEAVGLSRYALLRLEKG
EEEECCCEECCCCCEEEEEEEEEEHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEECCC
GIPVIRLGLQSTPEMELPGNIVAGPYHPAFRSLVNAAIFRDMATTLLSEARSEGRAVSFF
CCCEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LSPRDVSDFRGHKGENLSLLAERFRPLKIEVTNDPDYTRGWLTLRIGDRSISMHRTGLRP
ECCCCCHHHCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCEEEEHHCCCCC
ILS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coproporphyrinogen-III; O2 [C]

Specific reaction: coproporphyrinogen-III + O2 = protoporphyrinogen-IX + 2 CO2 [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With oxygen as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]