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The map label for this gene is rlmN

Identifier: 85859749

GI number: 85859749

Start: 1985830

End: 1986876

Strand: Direct

Name: rlmN

Synonym: SYN_01411

Alternate gene names: 85859749

Gene position: 1985830-1986876 (Clockwise)

Preceding gene: 85859748

Following gene: 85859755

Centisome position: 62.46

GC content: 51.67

Gene sequence:

>1047_bases
TTGCATATGAATCGAATCAATATCAGAGATATGTCACTTGAAGAGATCGAGTCCTTTATTTCCAGTTTGGGAAAGGAGAA
ATATCGGGCTCGACAGATCATGAAATGGCTTTACTCCCAGGGAGCAAAATCGTTCGACGAAATGACCACCCTGTCCAGGG
CCGTCCGCGACCAGTTGAATGAAATGGCCTGCATCACACTGCCGGAAATCGCCAGGGTTCAGCAATCATCCGACGGAACC
AGGAAGATCCTCTTTCGACTGCAAGACAACAGCTTCATCGAAAGCGTCCTGATCCCCGGAAAACATAACTGGACCGCCTG
CATCTCCACACAGGTCGGCTGCCATATGGGCTGCCGTTTCTGTTTCACCGCTCGACAGGGATTCCGGCGGAACCTTAAAC
CGTCGGAAATTACGGGCCAGTTGACGATGCTCCAGTTTTACCTTCCAGAAGGACCCGAGATCAAGAATATCGTCATGATG
GGCATGGGGGAACCACTGGCAAATTACAGAAACACCCTCAAGGCCATCCGCATCATCACCTCGGATTACGGACTTGGCTT
CTCCACCCGGAAAATTACCCTGTCCACCTCCGGCATCACTCCGATGATTGAACAACTGGGCCGTGATCTCTGTATTAATC
TGGCCATATCCCTGAACGCCCCGACCGATTCCATACGGAGTGAACTCATGCCTGTCAACAGGAAATATCCGCTTGACCGG
CTTCTTCAAGCCTGCCGGAATTATCCCATGCCAGGCCGCCGGATGCTGACCTTTGAATACATTCTCATCGATGGCGTAAA
TTCCTCACCAGCCCATGCAGAAATGCTCTGCCGGCTTTTGAAAGGCATCCGCTGCAAGCTGAATCTCATCCGCTTCAATG
AGTTCCCTGATTGCCCCTTTAAAACGCCTTCCGAGGAAACAGTCCTCGCCTTTCAGCAGATTCTTGTCAAGCATCATTAT
ACCGCCATCATCCGCGCAAGTCGGGGGCGAGACATTCTTGCCGCTTGCGGCCAATTGAGCGGCAAAGCCCTTGAGGAGAA
ACTCTAA

Upstream 100 bases:

>100_bases
TTAGCTTTCCGTTTCTGACAATCCTGCGGAAAAATTCAGGAAAGCAAAGGCAAATCGGCCCTTATGCCGTCGAGCGCCTT
TTCACAATTTTTAAAGAAAT

Downstream 100 bases:

>100_bases
AAAAGGTCCACGCAAAAATGCCCTTTTCAAACCCTACAATACAATTACAGAGGATTGAATTTATTCCCCGCGGCCTTCCC
GGGTATTTCATTCTTCTGGC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MHMNRINIRDMSLEEIESFISSLGKEKYRARQIMKWLYSQGAKSFDEMTTLSRAVRDQLNEMACITLPEIARVQQSSDGT
RKILFRLQDNSFIESVLIPGKHNWTACISTQVGCHMGCRFCFTARQGFRRNLKPSEITGQLTMLQFYLPEGPEIKNIVMM
GMGEPLANYRNTLKAIRIITSDYGLGFSTRKITLSTSGITPMIEQLGRDLCINLAISLNAPTDSIRSELMPVNRKYPLDR
LLQACRNYPMPGRRMLTFEYILIDGVNSSPAHAEMLCRLLKGIRCKLNLIRFNEFPDCPFKTPSEETVLAFQQILVKHHY
TAIIRASRGRDILAACGQLSGKALEEKL

Sequences:

>Translated_348_residues
MHMNRINIRDMSLEEIESFISSLGKEKYRARQIMKWLYSQGAKSFDEMTTLSRAVRDQLNEMACITLPEIARVQQSSDGT
RKILFRLQDNSFIESVLIPGKHNWTACISTQVGCHMGCRFCFTARQGFRRNLKPSEITGQLTMLQFYLPEGPEIKNIVMM
GMGEPLANYRNTLKAIRIITSDYGLGFSTRKITLSTSGITPMIEQLGRDLCINLAISLNAPTDSIRSELMPVNRKYPLDR
LLQACRNYPMPGRRMLTFEYILIDGVNSSPAHAEMLCRLLKGIRCKLNLIRFNEFPDCPFKTPSEETVLAFQQILVKHHY
TAIIRASRGRDILAACGQLSGKALEEKL
>Mature_348_residues
MHMNRINIRDMSLEEIESFISSLGKEKYRARQIMKWLYSQGAKSFDEMTTLSRAVRDQLNEMACITLPEIARVQQSSDGT
RKILFRLQDNSFIESVLIPGKHNWTACISTQVGCHMGCRFCFTARQGFRRNLKPSEITGQLTMLQFYLPEGPEIKNIVMM
GMGEPLANYRNTLKAIRIITSDYGLGFSTRKITLSTSGITPMIEQLGRDLCINLAISLNAPTDSIRSELMPVNRKYPLDR
LLQACRNYPMPGRRMLTFEYILIDGVNSSPAHAEMLCRLLKGIRCKLNLIRFNEFPDCPFKTPSEETVLAFQQILVKHHY
TAIIRASRGRDILAACGQLSGKALEEKL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=349, Percent_Identity=41.8338108882521, Blast_Score=298, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_SYNAS (Q2LUM5)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461951.1
- ProteinModelPortal:   Q2LUM5
- STRING:   Q2LUM5
- GeneID:   3882556
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_01411
- NMPDR:   fig|56780.10.peg.1895
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q2LUM5
- BioCyc:   SACI56780:SYN_01411-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39598; Mature: 39598

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMNRINIRDMSLEEIESFISSLGKEKYRARQIMKWLYSQGAKSFDEMTTLSRAVRDQLN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EMACITLPEIARVQQSSDGTRKILFRLQDNSFIESVLIPGKHNWTACISTQVGCHMGCRF
HEEEEECHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCCCCCCCEEEEECHHHHHHHHHH
CFTARQGFRRNLKPSEITGQLTMLQFYLPEGPEIKNIVMMGMGEPLANYRNTLKAIRIIT
HHHHHHHHHCCCCCCHHCCCEEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
SDYGLGFSTRKITLSTSGITPMIEQLGRDLCINLAISLNAPTDSIRSELMPVNRKYPLDR
CCCCCCCCCEEEEEECCCCCHHHHHCCHHEEEEEEEEECCCHHHHHHHHCCCCCCCCHHH
LLQACRNYPMPGRRMLTFEYILIDGVNSSPAHAEMLCRLLKGIRCKLNLIRFNEFPDCPF
HHHHHHCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCCC
KTPSEETVLAFQQILVKHHYTAIIRASRGRDILAACGQLSGKALEEKL
CCCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure
MHMNRINIRDMSLEEIESFISSLGKEKYRARQIMKWLYSQGAKSFDEMTTLSRAVRDQLN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EMACITLPEIARVQQSSDGTRKILFRLQDNSFIESVLIPGKHNWTACISTQVGCHMGCRF
HEEEEECHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCCCCCCCEEEEECHHHHHHHHHH
CFTARQGFRRNLKPSEITGQLTMLQFYLPEGPEIKNIVMMGMGEPLANYRNTLKAIRIIT
HHHHHHHHHCCCCCCHHCCCEEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
SDYGLGFSTRKITLSTSGITPMIEQLGRDLCINLAISLNAPTDSIRSELMPVNRKYPLDR
CCCCCCCCCEEEEEECCCCCHHHHHCCHHEEEEEEEEECCCHHHHHHHHCCCCCCCCHHH
LLQACRNYPMPGRRMLTFEYILIDGVNSSPAHAEMLCRLLKGIRCKLNLIRFNEFPDCPF
HHHHHHCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCCC
KTPSEETVLAFQQILVKHHYTAIIRASRGRDILAACGQLSGKALEEKL
CCCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA