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The map label for this gene is hup [H]

Identifier: 85859156

GI number: 85859156

Start: 1331775

End: 1332047

Strand: Direct

Name: hup [H]

Synonym: SYN_02859

Alternate gene names: 85859156

Gene position: 1331775-1332047 (Clockwise)

Preceding gene: 85859155

Following gene: 85859157

Centisome position: 41.89

GC content: 46.52

Gene sequence:

>273_bases
ATGACGAAGGCGGAATTGATTTCGGTAATAGCAGAAGAAGCAGGCATTACAAAGGCCTCAGCGTCGAAAGCTCTGGAAAG
TTATGTAAATACGGTAGCGAAGGAACTGAACAAAACTGGGAAACTGGGGCTGGTCGGCTTCGGAACTTTTTCCGTCGTCA
TGAGGGAAGCCCGCGAGGGTAGAAATCCTCAAACGGGCGATATCATCAAGATTCCGGCCAAGAAAGTCGTCAAATTCAAA
CCGGGAAAGGAATTGTCGGAAAAAATCAAATAA

Upstream 100 bases:

>100_bases
TTCAGCGTATCCGGGTGTCGCTTGAACTTTTCTGGGCGGCAGATATTCGGCATTTCACTCTAACCATTTCATTACAAAAT
ATCGAAGGGGGATTTGAAGG

Downstream 100 bases:

>100_bases
ATTTTCTGTTGCGTTTTTCGAGTCTGGAGAAAGCCCCCGGAAGAATAATTTCGGGGGCTTTCTTGCGTTTACCGGTTATC
TTTGATAAAGGACGGAAAAA

Product: DNA-binding protein HU

Products: NA

Alternate protein names: DNA-binding protein II; HB [H]

Number of amino acids: Translated: 90; Mature: 89

Protein sequence:

>90_residues
MTKAELISVIAEEAGITKASASKALESYVNTVAKELNKTGKLGLVGFGTFSVVMREAREGRNPQTGDIIKIPAKKVVKFK
PGKELSEKIK

Sequences:

>Translated_90_residues
MTKAELISVIAEEAGITKASASKALESYVNTVAKELNKTGKLGLVGFGTFSVVMREAREGRNPQTGDIIKIPAKKVVKFK
PGKELSEKIK
>Mature_89_residues
TKAELISVIAEEAGITKASASKALESYVNTVAKELNKTGKLGLVGFGTFSVVMREAREGRNPQTGDIIKIPAKKVVKFKP
GKELSEKIK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=47.1910112359551, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=47.7777777777778, Blast_Score=77, Evalue=3e-16,
Organism=Escherichia coli, GI1787141, Length=89, Percent_Identity=38.2022471910112, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=3e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9676; Mature: 9545

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAELISVIAEEAGITKASASKALESYVNTVAKELNKTGKLGLVGFGTFSVVMREAREG
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCC
RNPQTGDIIKIPAKKVVKFKPGKELSEKIK
CCCCCCCEEECCHHHHEECCCCCHHHHCCC
>Mature Secondary Structure 
TKAELISVIAEEAGITKASASKALESYVNTVAKELNKTGKLGLVGFGTFSVVMREAREG
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCC
RNPQTGDIIKIPAKKVVKFKPGKELSEKIK
CCCCCCCEEECCHHHHEECCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644313 [H]