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The map label for this gene is thiH [C]

Identifier: 85858921

GI number: 85858921

Start: 1100909

End: 1101964

Strand: Direct

Name: thiH [C]

Synonym: SYN_02134

Alternate gene names: 85858921

Gene position: 1100909-1101964 (Clockwise)

Preceding gene: 85858920

Following gene: 85858922

Centisome position: 34.63

GC content: 56.44

Gene sequence:

>1056_bases
ATGCCGCGACAGGCGGCGCCGATGACGGCACCGGGGAAGATTACAGATTGTCTGGAGCGGCTTAAGGGATCGGGTCTACC
TCAGCGGGATGACCTGATCGCCCTTCTTTCATGCGCTGATGAGAAAGAACTGAAGCCGTTGTTTCTGGCGGCCGACGCCC
GGAGAAAAGCAGCTGTCGGAGATGTCGTTCATCTTCGCGGCATCGTTGAATTTTCAAATTATTGTGAGCGGAACTGCCTC
TACTGTGGACTTCGGCGGGTAAACCGACAGTTGAAACGCTACCGGATGTCGAACCGGGAGATCCTGGAGGCGGCCTTTGC
CGTCAAAGCGGCTAAGATCCCCACGCTGGTCCTTCAATCCGGCGAAGATTCATTTTACACGACAGAGAAGCTCTGCAGTC
TGATTCAATCCATCAAAGCGGAAACCGGTCTGGTCGTTACCCTGTCCCTGGGGGAGCGCCCCCGGAATGATTATCATGCG
TTCCGGGAAGCCGGCGCAGACCGTTATCTGCTGAAACATGAAACAGCCAGTCCCTCTCTTTACGGACGACTCCACCCGGA
TTCCCGACAGGAAAACCGTCTGCAGTGCCTGAAATGGCTGAAGGAATTGCGCTATGAGACGGGCGCGGGTATCATGGTGG
GACTGCCCGGCCAGGATGCCGCCGTTCTGGCTGATGACATTCTCCTGATTTCTTCTCTGAAAGCCGATATGGCCGGTATC
GGGCCATTCCTGCCTCATCCGCAGACGCCTCTGGCGGCCTGTCCTTCAGGCATGGTTCTGCCCGTTCTCACCGTTCTGGC
CCTCGTCCGTCTTGCATGTCCCACCATCAATCTTCCCGCCACCACCGCCCTGTCCGTCCTCCATCCGGAGGGAAGGAAAA
AGGCGCTCGCTGTCGGAGCCAATGTTGTCATGCCGGATTTTACCCCGCCGGCTTACCGGCGACTTTATGATCTTTATCCC
GGCCGAACGGCAGGTTCCGAAGATACTGAATTAATCATGGAAACCCTGAAGAAGGAAATAATCTGTTGCGGAAGGACCCT
CCAGGTGACAACTTGA

Upstream 100 bases:

>100_bases
ACGGCGGAAAGGTCGAAGCGACGCGGGCATGCGAACCTCGACTGCCGAAAAACCGTCAATAAAGCCGGTAAAGGGGCAAC
AGTGATATGGAATTGGAAGA

Downstream 100 bases:

>100_bases
TTCAACTCAGTCTCTACATCGTCTGTAATGATTTCCACGAAGAAAAAAGTACAAAAAAGATCCGGGAACGCTCATCACTT
ATCAAATACACGGCCGCCTC

Product: biotin synthase

Products: biotin

Alternate protein names: Radical SAM Domain Protein; Radical SAM Domain-Containing Protein; Thiamine Biosynthesis Protein ThiH; Biotin Synthetase; Iron-Only Hydrogenase Maturation Protein HydE; ThiH Protein; Biotin Biosynthesis-Related Protein; Biotin Synthase And Related S; Fe-Hydrogenase Assembly Protein Radical Protein; Biotin Synthase Family; Thiazole Biosynthesis Protein ThiH; Iron-Only Hydrogenase Maturation Protein; Radical SAM; Thiamine Biosynthesis

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MPRQAAPMTAPGKITDCLERLKGSGLPQRDDLIALLSCADEKELKPLFLAADARRKAAVGDVVHLRGIVEFSNYCERNCL
YCGLRRVNRQLKRYRMSNREILEAAFAVKAAKIPTLVLQSGEDSFYTTEKLCSLIQSIKAETGLVVTLSLGERPRNDYHA
FREAGADRYLLKHETASPSLYGRLHPDSRQENRLQCLKWLKELRYETGAGIMVGLPGQDAAVLADDILLISSLKADMAGI
GPFLPHPQTPLAACPSGMVLPVLTVLALVRLACPTINLPATTALSVLHPEGRKKALAVGANVVMPDFTPPAYRRLYDLYP
GRTAGSEDTELIMETLKKEIICCGRTLQVTT

Sequences:

>Translated_351_residues
MPRQAAPMTAPGKITDCLERLKGSGLPQRDDLIALLSCADEKELKPLFLAADARRKAAVGDVVHLRGIVEFSNYCERNCL
YCGLRRVNRQLKRYRMSNREILEAAFAVKAAKIPTLVLQSGEDSFYTTEKLCSLIQSIKAETGLVVTLSLGERPRNDYHA
FREAGADRYLLKHETASPSLYGRLHPDSRQENRLQCLKWLKELRYETGAGIMVGLPGQDAAVLADDILLISSLKADMAGI
GPFLPHPQTPLAACPSGMVLPVLTVLALVRLACPTINLPATTALSVLHPEGRKKALAVGANVVMPDFTPPAYRRLYDLYP
GRTAGSEDTELIMETLKKEIICCGRTLQVTT
>Mature_350_residues
PRQAAPMTAPGKITDCLERLKGSGLPQRDDLIALLSCADEKELKPLFLAADARRKAAVGDVVHLRGIVEFSNYCERNCLY
CGLRRVNRQLKRYRMSNREILEAAFAVKAAKIPTLVLQSGEDSFYTTEKLCSLIQSIKAETGLVVTLSLGERPRNDYHAF
REAGADRYLLKHETASPSLYGRLHPDSRQENRLQCLKWLKELRYETGAGIMVGLPGQDAAVLADDILLISSLKADMAGIG
PFLPHPQTPLAACPSGMVLPVLTVLALVRLACPTINLPATTALSVLHPEGRKKALAVGANVVMPDFTPPAYRRLYDLYPG
RTAGSEDTELIMETLKKEIICCGRTLQVTT

Specific function: Required For The Synthesis Of The Thiazole Moiety Of Thiamine. [C]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.6

Molecular weight: Translated: 38540; Mature: 38409

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQAAPMTAPGKITDCLERLKGSGLPQRDDLIALLSCADEKELKPLFLAADARRKAAVG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHH
DVVHLRGIVEFSNYCERNCLYCGLRRVNRQLKRYRMSNREILEAAFAVKAAKIPTLVLQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEC
GEDSFYTTEKLCSLIQSIKAETGLVVTLSLGERPRNDYHAFREAGADRYLLKHETASPSL
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCE
YGRLHPDSRQENRLQCLKWLKELRYETGAGIMVGLPGQDAAVLADDILLISSLKADMAGI
EEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
GPFLPHPQTPLAACPSGMVLPVLTVLALVRLACPTINLPATTALSVLHPEGRKKALAVGA
CCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCEEEECC
NVVMPDFTPPAYRRLYDLYPGRTAGSEDTELIMETLKKEIICCGRTLQVTT
CEECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
PRQAAPMTAPGKITDCLERLKGSGLPQRDDLIALLSCADEKELKPLFLAADARRKAAVG
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHH
DVVHLRGIVEFSNYCERNCLYCGLRRVNRQLKRYRMSNREILEAAFAVKAAKIPTLVLQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEC
GEDSFYTTEKLCSLIQSIKAETGLVVTLSLGERPRNDYHAFREAGADRYLLKHETASPSL
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCE
YGRLHPDSRQENRLQCLKWLKELRYETGAGIMVGLPGQDAAVLADDILLISSLKADMAGI
EEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
GPFLPHPQTPLAACPSGMVLPVLTVLALVRLACPTINLPATTALSVLHPEGRKKALAVGA
CCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCEEEECC
NVVMPDFTPPAYRRLYDLYPGRTAGSEDTELIMETLKKEIICCGRTLQVTT
CEECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA