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The map label for this gene is thiH

Identifier: 85858920

GI number: 85858920

Start: 1099372

End: 1100766

Strand: Direct

Name: thiH

Synonym: SYN_02133

Alternate gene names: 85858920

Gene position: 1099372-1100766 (Clockwise)

Preceding gene: 85858919

Following gene: 85858921

Centisome position: 34.58

GC content: 55.48

Gene sequence:

>1395_bases
ATGAAGGATTTTATCAGAGACACGGAGATTGAAGAACTGCTTCGAAAGGCAGGCTCTCCCGACAGAAAACAGGTTCTGGA
GATCATTGCGAAGTCCCGGGAATTAAAAGGTCTGGCGCCGGAAGAAACGGCCGTCCTTCTGCAGACGGACGATCCGGAGC
TGATTCTGGCGATTTACGAAACGGCCAGAGAAATCAAACAGAGCATCTACGGCAACCGGCTTGTCCTTTTCGCTCCCCTG
TATATCGCCAATTACTGTTCGAATAACTGCCTTTACTGTGGTTTCCGGAGAGACAACAAGGAGCTCAACCGGGTTGCCCT
TTCCATGGAGCAGGTCGCAGAGGAAGTTCGGGCCATCGAAAGAGAGGGGCATAAACGACTGCTGATGCTCTGCGGCGAAC
ATCCCAGTCGCTCCAGCCTGGATTATTTCATCGAGGCGATCGAAACGGCCTATGCCACCCATACCGAAGAAGGCGGCGAA
ATCCGGCGGATCAACGTTGAAATCGCTCCGATGAACGTTCCCGACTTTAAACGCCTCAAGGCGGCCAATATCGGAACCTA
TGTTCTTTTCCAGGAAACCTACCATCATGAAACCTACAGGGCCATGCATCCCCACGGTCCCAAGGCCGATTTCGCCTGGC
GGCTCACGGCCATGCATCGGGCGCAGGAAGCCGGGATTGACGATGTCGGGCTGGGCGCCCTTTTCGGGCTGTACGATTAC
CGTTATGAAGTTCTCGGCCTTCTGATCCATGCCCGGACTCTGGAGGCCGACTGCGGTGTAGGGCCCCACACCATTTCCAT
TCCCCGGCTGGAACCGGCATTCAATGCCCCAGCGGCCTCATCACCGCCCCGTCCGGTGAGCGATGAGGAATTCAAGAAGC
TGGTGGCCATCATCCGGATGGCGGTTCCCTATACGGGCATGATCTTAAGCACCCGTGAATCGCCGGAAATCCGCACCGCC
GTCTTTGAACTGGGTATCTCGCAGATCAGCGCGGGGTCGAGAACGAATCCCGGGGGGTACGCGAAAGATTCAAGCGAAGG
ATTCCGCGCCGCTCAGTTCAACCTCGGGGATACGAGAACTGCTGATGAGGTTATCCACGATATTACAACCAGGGGGCACA
TCCCCAGTTTCTGTACCGCCTGCTACCGCCTCGGAAGGACGGGCAGGGATTTCATGGATCTGGCGAAACCCGGTCTGATC
CAGCATTTTTGCCGAAGCAACGCCATGATGACCTTCAAGGAATATCTCATGGATTACGCTTCTCCCAGGACTAAGGAAGT
GGGAGAGCAACTTCTGGCCGGGCTTGTCGAGAGAACGGAAAAACTGGAAACCCGACAGATTCTGATAGAGCGCCTCGGGC
GAATCGAAAGAGGCGAACGGGATCTCTTTATCTGA

Upstream 100 bases:

>100_bases
ATGAAATCGGCGCCATGACGGGGAAGCTTGGCTCCCTGGATGGTGTCAAAGTCAAATCGGCACTTCAGAAAGTTGCGTAA
TTTCAGGAAAGGGCGAATTC

Downstream 100 bases:

>100_bases
TGAGATTTACCCTCTTTGAGGCATTTCAATGGATGATCAGGGACGGCGGAAAGGTCGAAGCGACGCGGGCATGCGAACCT
CGACTGCCGAAAAACCGTCA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKDFIRDTEIEELLRKAGSPDRKQVLEIIAKSRELKGLAPEETAVLLQTDDPELILAIYETAREIKQSIYGNRLVLFAPL
YIANYCSNNCLYCGFRRDNKELNRVALSMEQVAEEVRAIEREGHKRLLMLCGEHPSRSSLDYFIEAIETAYATHTEEGGE
IRRINVEIAPMNVPDFKRLKAANIGTYVLFQETYHHETYRAMHPHGPKADFAWRLTAMHRAQEAGIDDVGLGALFGLYDY
RYEVLGLLIHARTLEADCGVGPHTISIPRLEPAFNAPAASSPPRPVSDEEFKKLVAIIRMAVPYTGMILSTRESPEIRTA
VFELGISQISAGSRTNPGGYAKDSSEGFRAAQFNLGDTRTADEVIHDITTRGHIPSFCTACYRLGRTGRDFMDLAKPGLI
QHFCRSNAMMTFKEYLMDYASPRTKEVGEQLLAGLVERTEKLETRQILIERLGRIERGERDLFI

Sequences:

>Translated_464_residues
MKDFIRDTEIEELLRKAGSPDRKQVLEIIAKSRELKGLAPEETAVLLQTDDPELILAIYETAREIKQSIYGNRLVLFAPL
YIANYCSNNCLYCGFRRDNKELNRVALSMEQVAEEVRAIEREGHKRLLMLCGEHPSRSSLDYFIEAIETAYATHTEEGGE
IRRINVEIAPMNVPDFKRLKAANIGTYVLFQETYHHETYRAMHPHGPKADFAWRLTAMHRAQEAGIDDVGLGALFGLYDY
RYEVLGLLIHARTLEADCGVGPHTISIPRLEPAFNAPAASSPPRPVSDEEFKKLVAIIRMAVPYTGMILSTRESPEIRTA
VFELGISQISAGSRTNPGGYAKDSSEGFRAAQFNLGDTRTADEVIHDITTRGHIPSFCTACYRLGRTGRDFMDLAKPGLI
QHFCRSNAMMTFKEYLMDYASPRTKEVGEQLLAGLVERTEKLETRQILIERLGRIERGERDLFI
>Mature_464_residues
MKDFIRDTEIEELLRKAGSPDRKQVLEIIAKSRELKGLAPEETAVLLQTDDPELILAIYETAREIKQSIYGNRLVLFAPL
YIANYCSNNCLYCGFRRDNKELNRVALSMEQVAEEVRAIEREGHKRLLMLCGEHPSRSSLDYFIEAIETAYATHTEEGGE
IRRINVEIAPMNVPDFKRLKAANIGTYVLFQETYHHETYRAMHPHGPKADFAWRLTAMHRAQEAGIDDVGLGALFGLYDY
RYEVLGLLIHARTLEADCGVGPHTISIPRLEPAFNAPAASSPPRPVSDEEFKKLVAIIRMAVPYTGMILSTRESPEIRTA
VFELGISQISAGSRTNPGGYAKDSSEGFRAAQFNLGDTRTADEVIHDITTRGHIPSFCTACYRLGRTGRDFMDLAKPGLI
QHFCRSNAMMTFKEYLMDYASPRTKEVGEQLLAGLVERTEKLETRQILIERLGRIERGERDLFI

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=334, Percent_Identity=31.7365269461078, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 52407; Mature: 52407

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDFIRDTEIEELLRKAGSPDRKQVLEIIAKSRELKGLAPEETAVLLQTDDPELILAIYE
CCCHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEHHHHH
TAREIKQSIYGNRLVLFAPLYIANYCSNNCLYCGFRRDNKELNRVALSMEQVAEEVRAIE
HHHHHHHHHCCCEEEEEHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
REGHKRLLMLCGEHPSRSSLDYFIEAIETAYATHTEEGGEIRRINVEIAPMNVPDFKRLK
HCCCCEEEHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHH
AANIGTYVLFQETYHHETYRAMHPHGPKADFAWRLTAMHRAQEAGIDDVGLGALFGLYDY
HCCCCEEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RYEVLGLLIHARTLEADCGVGPHTISIPRLEPAFNAPAASSPPRPVSDEEFKKLVAIIRM
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AVPYTGMILSTRESPEIRTAVFELGISQISAGSRTNPGGYAKDSSEGFRAAQFNLGDTRT
HCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCH
ADEVIHDITTRGHIPSFCTACYRLGRTGRDFMDLAKPGLIQHFCRSNAMMTFKEYLMDYA
HHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHC
SPRTKEVGEQLLAGLVERTEKLETRQILIERLGRIERGERDLFI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKDFIRDTEIEELLRKAGSPDRKQVLEIIAKSRELKGLAPEETAVLLQTDDPELILAIYE
CCCHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEHHHHH
TAREIKQSIYGNRLVLFAPLYIANYCSNNCLYCGFRRDNKELNRVALSMEQVAEEVRAIE
HHHHHHHHHCCCEEEEEHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
REGHKRLLMLCGEHPSRSSLDYFIEAIETAYATHTEEGGEIRRINVEIAPMNVPDFKRLK
HCCCCEEEHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHH
AANIGTYVLFQETYHHETYRAMHPHGPKADFAWRLTAMHRAQEAGIDDVGLGALFGLYDY
HCCCCEEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RYEVLGLLIHARTLEADCGVGPHTISIPRLEPAFNAPAASSPPRPVSDEEFKKLVAIIRM
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AVPYTGMILSTRESPEIRTAVFELGISQISAGSRTNPGGYAKDSSEGFRAAQFNLGDTRT
HCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCH
ADEVIHDITTRGHIPSFCTACYRLGRTGRDFMDLAKPGLIQHFCRSNAMMTFKEYLMDYA
HHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHC
SPRTKEVGEQLLAGLVERTEKLETRQILIERLGRIERGERDLFI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]