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The map label for this gene is cysL [H]

Identifier: 85858908

GI number: 85858908

Start: 1087753

End: 1088712

Strand: Direct

Name: cysL [H]

Synonym: SYN_02121

Alternate gene names: 85858908

Gene position: 1087753-1088712 (Clockwise)

Preceding gene: 85858907

Following gene: 85858909

Centisome position: 34.21

GC content: 53.44

Gene sequence:

>960_bases
ATGATAGCAGTCGCAGTCATGGCATACGACGGTTTTCGGAACATTACCATTCAGCAGCTTGAAACCCTGGTCGCTCTCGT
CGAAGAACGGAGCTTCAGCCGGGCTTCCCGGCGGGTTTGTCTCACCCAGCCTTCCCTGACCAAACATGTAAAAAACATGG
AAGAGGTCCTGAATGTCCGACTGGTCAATCGGGAGCGCATGGGTATTTCCCTGACTCCGGAGGGAAAGGTTCTTTACGAT
TATGCACGGAAACTGCTTCATCTGAGGGAAGAGGCCGGGGAACGGCTGGTCCGGACTCAGGATCATACGGGTGGTGTTAT
CGACATGCACGCCAGTACGATCCCCGCAACCTATATTCTCCCTTATGTCCTCAGTGATTTCCGTAAGATCCATCCGGATA
TCCGGGTGAATGTCCAGATGGCGGACAGCGAGGAGTCCATGGACGCGATCCTGAACCAGCAGTCGGAGATCGGCATCATC
GGGAAGAAGCCGTTGAATCAGAAACTGAACGTAGAGCCTCTCTGGGAAGACCGACTGATTCTTGCAGTTCCCCGAGGGCA
TCGTATGGCTGGCAGGGGAATGGTCACCTGGGCGGAGCTGGCGGGGGAACCCTTTGTCGTTCGGGAAAAAGGGTCTGCCA
CGCGTGAGGTCTTCGAGCGATATCTCCGGGATGCGTTAAACTGGACACTTTCGAGATTGCATATCGCTGTTGAACTCGGG
AGTTCCGAGGCAGTAAAGGAAGCGGTGATTGCGGGACTTGGAATTTCCATGATTTCCATCCATGCAGTTGAGCGGGAGCT
TCTTTCCGGTCTTCTGTACGAAGTGCCCGTTGAGGACTGCCGCATCGAACGCTCTTTTTATCTGATTTATCGTCGGCAGC
TCGCTTTGATGCGTCACCACCAGATTTTTCTGGATTTTGTCCGAAGTCATAAAATTCATCGGGAACAGCTCCCTGCTTAG

Upstream 100 bases:

>100_bases
GATAGCGGCATGATCATGAGAATGCAAGACTATAAGAGAAAGAAGAAAGGATGTCAAATTCATCTGAGTGCTTCAAACGG
ATTGATTATTCCTTTCGGTT

Downstream 100 bases:

>100_bases
AAGGTTCAACGTTCCAAATTCTATGTCTCTCATCGGGAAATGCTATCCTGGAACTTCGAACTTTGAAGGTGGAATTCAGC
GTTCCACGCCGTCGGGAAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MIAVAVMAYDGFRNITIQQLETLVALVEERSFSRASRRVCLTQPSLTKHVKNMEEVLNVRLVNRERMGISLTPEGKVLYD
YARKLLHLREEAGERLVRTQDHTGGVIDMHASTIPATYILPYVLSDFRKIHPDIRVNVQMADSEESMDAILNQQSEIGII
GKKPLNQKLNVEPLWEDRLILAVPRGHRMAGRGMVTWAELAGEPFVVREKGSATREVFERYLRDALNWTLSRLHIAVELG
SSEAVKEAVIAGLGISMISIHAVERELLSGLLYEVPVEDCRIERSFYLIYRRQLALMRHHQIFLDFVRSHKIHREQLPA

Sequences:

>Translated_319_residues
MIAVAVMAYDGFRNITIQQLETLVALVEERSFSRASRRVCLTQPSLTKHVKNMEEVLNVRLVNRERMGISLTPEGKVLYD
YARKLLHLREEAGERLVRTQDHTGGVIDMHASTIPATYILPYVLSDFRKIHPDIRVNVQMADSEESMDAILNQQSEIGII
GKKPLNQKLNVEPLWEDRLILAVPRGHRMAGRGMVTWAELAGEPFVVREKGSATREVFERYLRDALNWTLSRLHIAVELG
SSEAVKEAVIAGLGISMISIHAVERELLSGLLYEVPVEDCRIERSFYLIYRRQLALMRHHQIFLDFVRSHKIHREQLPA
>Mature_319_residues
MIAVAVMAYDGFRNITIQQLETLVALVEERSFSRASRRVCLTQPSLTKHVKNMEEVLNVRLVNRERMGISLTPEGKVLYD
YARKLLHLREEAGERLVRTQDHTGGVIDMHASTIPATYILPYVLSDFRKIHPDIRVNVQMADSEESMDAILNQQSEIGII
GKKPLNQKLNVEPLWEDRLILAVPRGHRMAGRGMVTWAELAGEPFVVREKGSATREVFERYLRDALNWTLSRLHIAVELG
SSEAVKEAVIAGLGISMISIHAVERELLSGLLYEVPVEDCRIERSFYLIYRRQLALMRHHQIFLDFVRSHKIHREQLPA

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=301, Percent_Identity=32.5581395348837, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI145693105, Length=283, Percent_Identity=27.9151943462898, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082132, Length=262, Percent_Identity=30.9160305343511, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=27.2, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790399, Length=303, Percent_Identity=25.0825082508251, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788748, Length=300, Percent_Identity=24.6666666666667, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI157672245, Length=204, Percent_Identity=27.9411764705882, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786401, Length=305, Percent_Identity=23.2786885245902, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787128, Length=324, Percent_Identity=24.6913580246914, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787806, Length=245, Percent_Identity=24.4897959183673, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081904, Length=204, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=184, Percent_Identity=25.5434782608696, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788626, Length=270, Percent_Identity=25.5555555555556, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790208, Length=249, Percent_Identity=20.8835341365462, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788296, Length=249, Percent_Identity=22.0883534136546, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787530, Length=259, Percent_Identity=24.7104247104247, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36509; Mature: 36509

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVAVMAYDGFRNITIQQLETLVALVEERSFSRASRRVCLTQPSLTKHVKNMEEVLNVR
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHH
LVNRERMGISLTPEGKVLYDYARKLLHLREEAGERLVRTQDHTGGVIDMHASTIPATYIL
EECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHH
PYVLSDFRKIHPDIRVNVQMADSEESMDAILNQQSEIGIIGKKPLNQKLNVEPLWEDRLI
HHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEE
LAVPRGHRMAGRGMVTWAELAGEPFVVREKGSATREVFERYLRDALNWTLSRLHIAVELG
EEECCCCCCCCCCCEEHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEC
SSEAVKEAVIAGLGISMISIHAVERELLSGLLYEVPVEDCRIERSFYLIYRRQLALMRHH
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
QIFLDFVRSHKIHREQLPA
HHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MIAVAVMAYDGFRNITIQQLETLVALVEERSFSRASRRVCLTQPSLTKHVKNMEEVLNVR
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHH
LVNRERMGISLTPEGKVLYDYARKLLHLREEAGERLVRTQDHTGGVIDMHASTIPATYIL
EECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHH
PYVLSDFRKIHPDIRVNVQMADSEESMDAILNQQSEIGIIGKKPLNQKLNVEPLWEDRLI
HHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEE
LAVPRGHRMAGRGMVTWAELAGEPFVVREKGSATREVFERYLRDALNWTLSRLHIAVELG
EEECCCCCCCCCCCEEHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEC
SSEAVKEAVIAGLGISMISIHAVERELLSGLLYEVPVEDCRIERSFYLIYRRQLALMRHH
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
QIFLDFVRSHKIHREQLPA
HHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]