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The map label for this gene is pit [H]

Identifier: 85858882

GI number: 85858882

Start: 1064338

End: 1065339

Strand: Direct

Name: pit [H]

Synonym: SYN_00387

Alternate gene names: 85858882

Gene position: 1064338-1065339 (Clockwise)

Preceding gene: 85858881

Following gene: 85858885

Centisome position: 33.48

GC content: 50.3

Gene sequence:

>1002_bases
ATGCTTGATTCTCTTTTCTTTGTTATCTTTTTGGTCGGAGTGGCTCTGATTTTTGACTTCATCAACGGCTTTCACGATTC
TGCAAACTCCATTGCCACCGTGGTCTCCACCCGAGTACTCTCACCGAAATATGCAGTCCTCTGGGCGGCATTTTTCAATT
TTATAGCCTTTCTTTTTATCGGTGTCCCGGTTGCCAAAACAATCGGAACCGGCATCATCGACCCGCGCATTATTGATAAT
CTGCTCATCTTCGCCACACTGAGTGCGGCAATTATCTGGAATCTCATCACCTGGTATTTTGGATTGCCGAGTAGTTCATC
CCACGCACTGATCGGAGGGCTGATTGGCGCCGGGATCGTAAAAGGGGGAACAGAAGTCCTTGTCTGGAAGGGCATCACCA
AAACAACCATCTTCATTGTTGTTTCGCCGGTCATCGGCATGATGATTGGATTCGTGCTTATGCTACTTGTTCTGCACCTG
TTCCGCAGGGCTCATCCTACTAAAATGGATCATGTATTCCGAAAAATGCAGCTTTTTTCGGCCGCTGTTTACAGTTTGGG
GCACGGCATGAATGACGCACAAAAAACCATGGGTATTATTGCCATGGTCCTTTTCAGCAGTGGACTATTGGGGCCGACTT
TCCACATCCCCTTCTGGATCATCATTACGTGCCATGCAGCCATTGCACTGGGAACGGTTTCGGGAGGGTGGAGAATTGTA
AAAACGATGGGTACCCGCATCACAAAGCTTCGACCGATAGGCGGGTTTTGTGCGGAGACAGCAGCTGCCATGTCTATCAT
TGGAGCCTCTCTGGCGGGCATTCCGGTCAGCACAACCCACACCATCACGGGAGCGATATTGGGAGTCGGTTCCACAATTC
GATTTAGTGCCGTCCGTTGGGGGATCGCCGGAACGATAATCTGGGCCTGGGTTCTCACCATTCCGATTTCGGCACTCATT
TCCGCCCTGATTTACTGTGTCGCGCGGTCCTTGTCCCAGTAG

Upstream 100 bases:

>100_bases
GTCGAACTCATCAAGTGGAAGGAAATTTTTGAGCGGATCGAAGAGGCTATTGATGTCTGCGAAGATGTATCCAATATTGT
CGAGGGGATCGTTCTGAAAA

Downstream 100 bases:

>100_bases
AATCCCTCTCTGATTTAAAAGATGCCGCCGATTTAAAAGAGCTCTGTTGGAGCTTGTCACTTCATTCGCTGCTTTCCCAA
AACCCCGCCGGAAACAATAA

Product: phosphate/sulphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MLDSLFFVIFLVGVALIFDFINGFHDSANSIATVVSTRVLSPKYAVLWAAFFNFIAFLFIGVPVAKTIGTGIIDPRIIDN
LLIFATLSAAIIWNLITWYFGLPSSSSHALIGGLIGAGIVKGGTEVLVWKGITKTTIFIVVSPVIGMMIGFVLMLLVLHL
FRRAHPTKMDHVFRKMQLFSAAVYSLGHGMNDAQKTMGIIAMVLFSSGLLGPTFHIPFWIIITCHAAIALGTVSGGWRIV
KTMGTRITKLRPIGGFCAETAAAMSIIGASLAGIPVSTTHTITGAILGVGSTIRFSAVRWGIAGTIIWAWVLTIPISALI
SALIYCVARSLSQ

Sequences:

>Translated_333_residues
MLDSLFFVIFLVGVALIFDFINGFHDSANSIATVVSTRVLSPKYAVLWAAFFNFIAFLFIGVPVAKTIGTGIIDPRIIDN
LLIFATLSAAIIWNLITWYFGLPSSSSHALIGGLIGAGIVKGGTEVLVWKGITKTTIFIVVSPVIGMMIGFVLMLLVLHL
FRRAHPTKMDHVFRKMQLFSAAVYSLGHGMNDAQKTMGIIAMVLFSSGLLGPTFHIPFWIIITCHAAIALGTVSGGWRIV
KTMGTRITKLRPIGGFCAETAAAMSIIGASLAGIPVSTTHTITGAILGVGSTIRFSAVRWGIAGTIIWAWVLTIPISALI
SALIYCVARSLSQ
>Mature_333_residues
MLDSLFFVIFLVGVALIFDFINGFHDSANSIATVVSTRVLSPKYAVLWAAFFNFIAFLFIGVPVAKTIGTGIIDPRIIDN
LLIFATLSAAIIWNLITWYFGLPSSSSHALIGGLIGAGIVKGGTEVLVWKGITKTTIFIVVSPVIGMMIGFVLMLLVLHL
FRRAHPTKMDHVFRKMQLFSAAVYSLGHGMNDAQKTMGIIAMVLFSSGLLGPTFHIPFWIIITCHAAIALGTVSGGWRIV
KTMGTRITKLRPIGGFCAETAAAMSIIGASLAGIPVSTTHTITGAILGVGSTIRFSAVRWGIAGTIIWAWVLTIPISALI
SALIYCVARSLSQ

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=160, Percent_Identity=33.75, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI5803173, Length=155, Percent_Identity=32.258064516129, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1789360, Length=221, Percent_Identity=36.6515837104072, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=36.8663594470046, Blast_Score=114, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI25146401, Length=169, Percent_Identity=29.585798816568, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=31.4814814814815, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17539280, Length=165, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=166, Percent_Identity=27.1084337349398, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI32566716, Length=163, Percent_Identity=27.6073619631902, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=170, Percent_Identity=27.6470588235294, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356511, Length=182, Percent_Identity=27.4725274725275, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35641; Mature: 35641

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSLFFVIFLVGVALIFDFINGFHDSANSIATVVSTRVLSPKYAVLWAAFFNFIAFLFI
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GVPVAKTIGTGIIDPRIIDNLLIFATLSAAIIWNLITWYFGLPSSSSHALIGGLIGAGIV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGGTEVLVWKGITKTTIFIVVSPVIGMMIGFVLMLLVLHLFRRAHPTKMDHVFRKMQLFS
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AAVYSLGHGMNDAQKTMGIIAMVLFSSGLLGPTFHIPFWIIITCHAAIALGTVSGGWRIV
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
KTMGTRITKLRPIGGFCAETAAAMSIIGASLAGIPVSTTHTITGAILGVGSTIRFSAVRW
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
GIAGTIIWAWVLTIPISALISALIYCVARSLSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDSLFFVIFLVGVALIFDFINGFHDSANSIATVVSTRVLSPKYAVLWAAFFNFIAFLFI
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GVPVAKTIGTGIIDPRIIDNLLIFATLSAAIIWNLITWYFGLPSSSSHALIGGLIGAGIV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGGTEVLVWKGITKTTIFIVVSPVIGMMIGFVLMLLVLHLFRRAHPTKMDHVFRKMQLFS
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AAVYSLGHGMNDAQKTMGIIAMVLFSSGLLGPTFHIPFWIIITCHAAIALGTVSGGWRIV
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
KTMGTRITKLRPIGGFCAETAAAMSIIGASLAGIPVSTTHTITGAILGVGSTIRFSAVRW
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
GIAGTIIWAWVLTIPISALISALIYCVARSLSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]