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The map label for this gene is ddl [H]

Identifier: 85858831

GI number: 85858831

Start: 1023515

End: 1024582

Strand: Direct

Name: ddl [H]

Synonym: SYN_00412

Alternate gene names: 85858831

Gene position: 1023515-1024582 (Clockwise)

Preceding gene: 85858830

Following gene: 85858832

Centisome position: 32.19

GC content: 56.84

Gene sequence:

>1068_bases
TTGGAAAGTCAAAATGAGACAAAATTGCGCGTAGGCATCATCATGGCCGGGTTGTCCTCTGAAAAAGAGGTGTCCCTGGA
GAGTGGGCGGAATATCTTCAGCAAGATTGACCGCCGGAAGTACAGCCCCATCCCCATCTTCATGGACAGCGATGCCGCCC
TGTGGGAAATACCCCTCAAGCTGCTGATGAGAAACTGCACCCGTGATGTCGAAGAGGATCTCGCCGAAGAGGCCGTGCGG
GTTCCTTATGAAGAGCTCAAAACCCGGGTTGATCTCGTTTTTCTTGCTCTGCACGGCAAGTATGGCGAGGATGGCTGCAT
GCAGGGGTTGCTGGAGCTTTTGAAAATCCCCTATACAGGCTCCGGCGTTCTTGCCTCTGCGCTGGGAATGGATAAGCTTG
CCAGCCGGCGGGTCCTGGAGATCAACGGAATCGATGTTCCCCGGACGGTCCCGGTTTTCCGCGGCGCATGGAAACCGGAG
GAGGAGAATGCCCTTTTTGAGCGGATCGAACAGGAAATCGGTTTTCCCTGCGTCGTCAAACCCTCTCGGGAAGGATGCAG
CACGGGCGTGAAGAAGGTAATGTCCCGGGCAGGGCTTTCCGATGCCCTCAACGGTGCTTTTCTCTGGGATTGCACGGCCC
TGGTGGAGGAATTCATCGACGGCATGGAAGTGACCTGCGGCATTCTGGGCGGCAATCCGCCAACGGCTTTGACCCCGTCG
GAAACCATCGCCACATCTGATGTGCTTTCGCTGGAGGATAAATTTCTCTATGGTCAGGGGGAAAACAAGACCCCGGCCCG
CCTTCCCGGGGAAACCCTGATGAAGATTCAGGAGGTGGCCGTAAAGGCCTTTGAAGCCCTTGATCTGAAGGGTTACGCCC
GGATCGATATGTTTGTCACATCCGCCGGCCGGGTTCTAGTGCTCGAACCCAACACCCAGCCGGGAATGACGCCGTCCACC
GTTCTCTTCCATCAGGCCGCCGCATCGGGAATAACCCAGACCGGACTGGTTGACCGAATTCTGGAGGCGGCTCTTTCCGT
TCACGCCGGCAAAAAAGGACCGCTGTAA

Upstream 100 bases:

>100_bases
GAATGCGGGCAGGACGGATCAGTTTTTTGGATTGTTCCAGAAATTAAAGGGAATGTTTTTAACCTTTTTGTCAGAAATTT
AAATAAGGAGCAGGCTATCC

Downstream 100 bases:

>100_bases
AAGTTTATGGGAATCCTCACGGCTTTCGCCGATTCTCTGGGAAGGAGAATCCTGCACGGGGTTGAGGAAATGGGCAGGAT
TATCCTTCTTTTTCTTTCTG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MESQNETKLRVGIIMAGLSSEKEVSLESGRNIFSKIDRRKYSPIPIFMDSDAALWEIPLKLLMRNCTRDVEEDLAEEAVR
VPYEELKTRVDLVFLALHGKYGEDGCMQGLLELLKIPYTGSGVLASALGMDKLASRRVLEINGIDVPRTVPVFRGAWKPE
EENALFERIEQEIGFPCVVKPSREGCSTGVKKVMSRAGLSDALNGAFLWDCTALVEEFIDGMEVTCGILGGNPPTALTPS
ETIATSDVLSLEDKFLYGQGENKTPARLPGETLMKIQEVAVKAFEALDLKGYARIDMFVTSAGRVLVLEPNTQPGMTPST
VLFHQAAASGITQTGLVDRILEAALSVHAGKKGPL

Sequences:

>Translated_355_residues
MESQNETKLRVGIIMAGLSSEKEVSLESGRNIFSKIDRRKYSPIPIFMDSDAALWEIPLKLLMRNCTRDVEEDLAEEAVR
VPYEELKTRVDLVFLALHGKYGEDGCMQGLLELLKIPYTGSGVLASALGMDKLASRRVLEINGIDVPRTVPVFRGAWKPE
EENALFERIEQEIGFPCVVKPSREGCSTGVKKVMSRAGLSDALNGAFLWDCTALVEEFIDGMEVTCGILGGNPPTALTPS
ETIATSDVLSLEDKFLYGQGENKTPARLPGETLMKIQEVAVKAFEALDLKGYARIDMFVTSAGRVLVLEPNTQPGMTPST
VLFHQAAASGITQTGLVDRILEAALSVHAGKKGPL
>Mature_355_residues
MESQNETKLRVGIIMAGLSSEKEVSLESGRNIFSKIDRRKYSPIPIFMDSDAALWEIPLKLLMRNCTRDVEEDLAEEAVR
VPYEELKTRVDLVFLALHGKYGEDGCMQGLLELLKIPYTGSGVLASALGMDKLASRRVLEINGIDVPRTVPVFRGAWKPE
EENALFERIEQEIGFPCVVKPSREGCSTGVKKVMSRAGLSDALNGAFLWDCTALVEEFIDGMEVTCGILGGNPPTALTPS
ETIATSDVLSLEDKFLYGQGENKTPARLPGETLMKIQEVAVKAFEALDLKGYARIDMFVTSAGRVLVLEPNTQPGMTPST
VLFHQAAASGITQTGLVDRILEAALSVHAGKKGPL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=361, Percent_Identity=33.5180055401662, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786280, Length=341, Percent_Identity=35.4838709677419, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 38675; Mature: 38675

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESQNETKLRVGIIMAGLSSEKEVSLESGRNIFSKIDRRKYSPIPIFMDSDAALWEIPLK
CCCCCCCEEEEEEEEECCCCCCCCCHHHCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHH
LLMRNCTRDVEEDLAEEAVRVPYEELKTRVDLVFLALHGKYGEDGCMQGLLELLKIPYTG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCC
SGVLASALGMDKLASRRVLEINGIDVPRTVPVFRGAWKPEEENALFERIEQEIGFPCVVK
CHHHHHHHCHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEC
PSREGCSTGVKKVMSRAGLSDALNGAFLWDCTALVEEFIDGMEVTCGILGGNPPTALTPS
CCHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
ETIATSDVLSLEDKFLYGQGENKTPARLPGETLMKIQEVAVKAFEALDLKGYARIDMFVT
HHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
SAGRVLVLEPNTQPGMTPSTVLFHQAAASGITQTGLVDRILEAALSVHAGKKGPL
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MESQNETKLRVGIIMAGLSSEKEVSLESGRNIFSKIDRRKYSPIPIFMDSDAALWEIPLK
CCCCCCCEEEEEEEEECCCCCCCCCHHHCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHH
LLMRNCTRDVEEDLAEEAVRVPYEELKTRVDLVFLALHGKYGEDGCMQGLLELLKIPYTG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCC
SGVLASALGMDKLASRRVLEINGIDVPRTVPVFRGAWKPEEENALFERIEQEIGFPCVVK
CHHHHHHHCHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEC
PSREGCSTGVKKVMSRAGLSDALNGAFLWDCTALVEEFIDGMEVTCGILGGNPPTALTPS
CCHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
ETIATSDVLSLEDKFLYGQGENKTPARLPGETLMKIQEVAVKAFEALDLKGYARIDMFVT
HHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
SAGRVLVLEPNTQPGMTPSTVLFHQAAASGITQTGLVDRILEAALSVHAGKKGPL
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA