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The map label for this gene is fliF [H]

Identifier: 85858817

GI number: 85858817

Start: 1006756

End: 1008285

Strand: Direct

Name: fliF [H]

Synonym: SYN_01470

Alternate gene names: 85858817

Gene position: 1006756-1008285 (Clockwise)

Preceding gene: 85858816

Following gene: 85858818

Centisome position: 31.67

GC content: 48.5

Gene sequence:

>1530_bases
ATGAATTCCTTCCTGCGGTTTTATGAAAACCTCCGGCAAAGCTTTGTGGAATTGCCGCCGGCAAGAAAGTGGTCTCTTCT
GTTTGTGGCAGGCATGACTTTGTCCGTTTTAGCGGCAATGGTGTATTTCGCAAACCGTCCTGAATACAAAGTCCTTTTTT
CCCATCTGTCCAGTGAAGACGCCTCCTCGATTCTGGCGAAGCTTCAGGAGAAAAAAATTTCCTATGAAATCAACTCTGCG
GGAGACACAATTTTCGTGCCTGCTGAAAAAGTTTCGGAACTTCGGCTGGAGCTTGCCGGATCGGGTCTCCCTCAGGGTGG
CGGGGTGGGTTTTGAGATCTTCGATCAGAAAACCCTGGGGGCAACCGAATTCGAACAGCAACTGAACTACAGGCGTGCGC
TTCAGGGTGAACTGGGCCGAACGATCAAGGGATTGGAGGAAATAGAATCCAGTCGGGTCCATATTGCCTTGCCAAGGGAA
TCCCTGTTTGTCAGTGAAGAGAAAAAACCGACGGCTTCTGTCACGCTGAAGTTGAAGCCGGGCCGGTCGCTGAGTTCATC
GCAGATCGAAGGGATCGCTCACCTGGTTGCCAGCAGTGTGGAAGGACTGAATGCCGGGGATGTCATTATTGTCGATAATA
AAGGCAATATTTTATCCAAACTGAAAGGCGAATCAGGCCTTTCAGGGTTATCCGGTTCGCAGATTGAATTCCAGCGGAAC
CTTGAAAAAGATACGGCTGCCAGGATCCAATCTCTTCTGGAAAATGTCGTCGGTCAGGGCAAGGCCGTGGTACGGGTGGC
TGCTGACCTGGATTTTCGAATGACTGAAAAAACGGAAGAATCCTATGATCCGGAATCCCCGGTCGTCAGGAGTACCCAGA
AACAGTCTGAAAAATCCGCAACCCCGGGAAAAGCCGATCAGAGCACGGGCCAGGAAAAACTGGATGAAATCATCAATTAC
GAGATCAACAAAACGGTCAGTAAAACGGTCATGCCCGTCGGCGAGATCAGGAAGCTTTCCATCGCTGTTCTCGTTGATGG
TACATATGTCAAGGATAAGCAGGGAAAAGAAATTTATCAGCCCCGATCCAAGAAGGAAATTGAATCCATCGAAGAGCTTG
TACGAACCTCCGCTGGTTTCAGCACCGCCCGGGGTGATCAAGTAACGGTTACGAACATGCCCTTCAAAAAACTTGATACG
GAAGACATGACAGGTGGGAATGGCTGGTTCGGGGAAAATATATCGACCTATTTCCCTCTGATTAAATATTTGCTCCTTCT
GGTCGTCGTTGTCCTTGTCTTCTTTTTTGTTATCCGTCCTCTGATCAGGGGAATAACCGGGCAGGTCACTGAAAAGGTCA
TGGAAAAAGAGGCTCTTCCAGAGGATATTATTGAATTGACGGGAGAAACTCAATCCCTGGACCTGCTGTCTGTAAAGGAA
CGGGAATTGACGGAAATTGAAGTTGTGAAGAAAATGGCCGTTGAAGATTCCAAATCTTTTGCCGAGCTGCTGCGTAACTG
GTTAAAGTAA

Upstream 100 bases:

>100_bases
GTCAGAAATAAAATCATTGATGCTTATCAGGAAGTCATGAGAATGCAGGTATAATAAAAATGAGAGATTCGGTATTCTTT
CAGCGTAAGCGTTTTTCCCC

Downstream 100 bases:

>100_bases
CATCTATGGTTATTGCAGATTAACTGCTGTACGATGGGGCAATGAACTGATCTGTTTCTTTAAGGTGCTTTATGGCAAGT
CTTACAAACGAAGAAAAGGC

Product: flagellar M-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNSFLRFYENLRQSFVELPPARKWSLLFVAGMTLSVLAAMVYFANRPEYKVLFSHLSSEDASSILAKLQEKKISYEINSA
GDTIFVPAEKVSELRLELAGSGLPQGGGVGFEIFDQKTLGATEFEQQLNYRRALQGELGRTIKGLEEIESSRVHIALPRE
SLFVSEEKKPTASVTLKLKPGRSLSSSQIEGIAHLVASSVEGLNAGDVIIVDNKGNILSKLKGESGLSGLSGSQIEFQRN
LEKDTAARIQSLLENVVGQGKAVVRVAADLDFRMTEKTEESYDPESPVVRSTQKQSEKSATPGKADQSTGQEKLDEIINY
EINKTVSKTVMPVGEIRKLSIAVLVDGTYVKDKQGKEIYQPRSKKEIESIEELVRTSAGFSTARGDQVTVTNMPFKKLDT
EDMTGGNGWFGENISTYFPLIKYLLLLVVVVLVFFFVIRPLIRGITGQVTEKVMEKEALPEDIIELTGETQSLDLLSVKE
RELTEIEVVKKMAVEDSKSFAELLRNWLK

Sequences:

>Translated_509_residues
MNSFLRFYENLRQSFVELPPARKWSLLFVAGMTLSVLAAMVYFANRPEYKVLFSHLSSEDASSILAKLQEKKISYEINSA
GDTIFVPAEKVSELRLELAGSGLPQGGGVGFEIFDQKTLGATEFEQQLNYRRALQGELGRTIKGLEEIESSRVHIALPRE
SLFVSEEKKPTASVTLKLKPGRSLSSSQIEGIAHLVASSVEGLNAGDVIIVDNKGNILSKLKGESGLSGLSGSQIEFQRN
LEKDTAARIQSLLENVVGQGKAVVRVAADLDFRMTEKTEESYDPESPVVRSTQKQSEKSATPGKADQSTGQEKLDEIINY
EINKTVSKTVMPVGEIRKLSIAVLVDGTYVKDKQGKEIYQPRSKKEIESIEELVRTSAGFSTARGDQVTVTNMPFKKLDT
EDMTGGNGWFGENISTYFPLIKYLLLLVVVVLVFFFVIRPLIRGITGQVTEKVMEKEALPEDIIELTGETQSLDLLSVKE
RELTEIEVVKKMAVEDSKSFAELLRNWLK
>Mature_509_residues
MNSFLRFYENLRQSFVELPPARKWSLLFVAGMTLSVLAAMVYFANRPEYKVLFSHLSSEDASSILAKLQEKKISYEINSA
GDTIFVPAEKVSELRLELAGSGLPQGGGVGFEIFDQKTLGATEFEQQLNYRRALQGELGRTIKGLEEIESSRVHIALPRE
SLFVSEEKKPTASVTLKLKPGRSLSSSQIEGIAHLVASSVEGLNAGDVIIVDNKGNILSKLKGESGLSGLSGSQIEFQRN
LEKDTAARIQSLLENVVGQGKAVVRVAADLDFRMTEKTEESYDPESPVVRSTQKQSEKSATPGKADQSTGQEKLDEIINY
EINKTVSKTVMPVGEIRKLSIAVLVDGTYVKDKQGKEIYQPRSKKEIESIEELVRTSAGFSTARGDQVTVTNMPFKKLDT
EDMTGGNGWFGENISTYFPLIKYLLLLVVVVLVFFFVIRPLIRGITGQVTEKVMEKEALPEDIIELTGETQSLDLLSVKE
RELTEIEVVKKMAVEDSKSFAELLRNWLK

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=424, Percent_Identity=38.6792452830189, Blast_Score=282, Evalue=3e-77,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 56402; Mature: 56402

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFLRFYENLRQSFVELPPARKWSLLFVAGMTLSVLAAMVYFANRPEYKVLFSHLSSED
CCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHH
ASSILAKLQEKKISYEINSAGDTIFVPAEKVSELRLELAGSGLPQGGGVGFEIFDQKTLG
HHHHHHHHHHHHHCEEEECCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
ATEFEQQLNYRRALQGELGRTIKGLEEIESSRVHIALPRESLFVSEEKKPTASVTLKLKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHEECCCCCCCEEEEEEECC
GRSLSSSQIEGIAHLVASSVEGLNAGDVIIVDNKGNILSKLKGESGLSGLSGSQIEFQRN
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCHHHHH
LEKDTAARIQSLLENVVGQGKAVVRVAADLDFRMTEKTEESYDPESPVVRSTQKQSEKSA
CCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCC
TPGKADQSTGQEKLDEIINYEINKTVSKTVMPVGEIRKLSIAVLVDGTYVKDKQGKEIYQ
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHEEEEEEEECCCEECCCCCCHHHC
PRSKKEIESIEELVRTSAGFSTARGDQVTVTNMPFKKLDTEDMTGGNGWFGENISTYFPL
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHH
IKYLLLLVVVVLVFFFVIRPLIRGITGQVTEKVMEKEALPEDIIELTGETQSLDLLSVKE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEECCH
RELTEIEVVKKMAVEDSKSFAELLRNWLK
HHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MNSFLRFYENLRQSFVELPPARKWSLLFVAGMTLSVLAAMVYFANRPEYKVLFSHLSSED
CCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHH
ASSILAKLQEKKISYEINSAGDTIFVPAEKVSELRLELAGSGLPQGGGVGFEIFDQKTLG
HHHHHHHHHHHHHCEEEECCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
ATEFEQQLNYRRALQGELGRTIKGLEEIESSRVHIALPRESLFVSEEKKPTASVTLKLKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHEECCCCCCCEEEEEEECC
GRSLSSSQIEGIAHLVASSVEGLNAGDVIIVDNKGNILSKLKGESGLSGLSGSQIEFQRN
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCHHHHH
LEKDTAARIQSLLENVVGQGKAVVRVAADLDFRMTEKTEESYDPESPVVRSTQKQSEKSA
CCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCC
TPGKADQSTGQEKLDEIINYEINKTVSKTVMPVGEIRKLSIAVLVDGTYVKDKQGKEIYQ
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHEEEEEEEECCCEECCCCCCHHHC
PRSKKEIESIEELVRTSAGFSTARGDQVTVTNMPFKKLDTEDMTGGNGWFGENISTYFPL
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHH
IKYLLLLVVVVLVFFFVIRPLIRGITGQVTEKVMEKEALPEDIIELTGETQSLDLLSVKE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEECCH
RELTEIEVVKKMAVEDSKSFAELLRNWLK
HHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 1551848 [H]