Click here to switch to the map view.

The map label for this gene is bclA [H]

Identifier: 85858649

GI number: 85858649

Start: 816312

End: 817886

Strand: Direct

Name: bclA [H]

Synonym: SYN_02896

Alternate gene names: 85858649

Gene position: 816312-817886 (Clockwise)

Preceding gene: 85858647

Following gene: 85858659

Centisome position: 25.68

GC content: 52.63

Gene sequence:

>1575_bases
ATGGCAAAGTTATACCCGGAGGAATTTTACAACTCAGCAGATTGGTTTGTAGACAGGCACGTCAGGGAGGGACGCGGCGA
CAATATTTGCGCTTATACGGACAAGGGCAATTACACCTATCGTGACATCCAGAAAATGGCCAACAAGATGGCTAACATGT
TCAAGGATCTGGATATCCGGATGGGCGACCGAATCATCATGCTTGTTCTGGACACCCCTTGGTTTTATTCCACTTTCTGG
GGTGCGGTAAAGATGGGGGCTGTCCCTGTCCCTTCCAGCACCATGCTTACGCCCGCCGACTATGAATATTACCTGAATGA
CAGCCAGGCGAGGACGCTTGTTGTTTCATCGAGACTTCTGCCGGTCGTCAATCAGATCGAAGAACTTCGTTTCCTTAAGA
ATATGATTGTTGTCGATGATGACGGTGTATTTTCCACGCCTTACCAGCAGATATATGCCTCAGCATCGGATGAATTTCAA
ACCGTGTTTACCTCGGCGGATGATGTTGCCTTCTGGCTTTATACATCCGGCACGACCGGCGGTCCGAAGGGGGCGGTTCA
TTCGCAGTCCGATATGCAGTACAGTGCTGAAGCTTATGGAAAGCATATCCTCGAGATTACGGAAAAGGACATCTGCTACT
CGGCAGCTCGACTCTTTTTCACCTACGGCCTCGGCAATGCCATGTTCTTCCCCATGTCTGTCGGCGCCGCGGCTGTCCTG
AACCCGGATCCCCCCGCTCCAGCCCATGTGTTCAGGTTGATCAAGACCTACAAGGCAACATTGTTCTTCGGCGTTCCAAC
GCTGTTCGGCCAGATGCTGATTACCCAGGATAAAATTGACGCGGAGAAGGGCGCTGGAGCTGACCCCAAGGATCATGATC
TGGTGTCGCTCCGAGCCTGTCCGTCTGCCGGCGAGGCGCTTCCCCCCGATCTGTACCATAAGTTCAAGGCCCGCTACGGC
GTGGAAATCCTTGACGGACCGGGTTCAACGGAAATGCTCCATATCTATCTGTCCAACAAGCTGGGCGACGTCAAACCAGG
TTCCAGTGGAAAGCCTGTCCCCGGCTATGAAGAGAAGATCATGGATGAAGAAGGCAAAAATGAACTGCCTGACGGTGAAG
TCGGAAACCTCTGGATCAAAGGGAGGAGTTCACTCCGTTATTACTGGAGAAAGAGAGACAAGACAGCCGCTACCGTCATC
GGTGAGTGGGTCAATTCTGGTGACAAGTATTACAAGGATGCGGAAGGGTACTACTGGCCGTCAGGCCGGGCCGACGACAT
GCTGAAAGTCGGCGGCATCTGGGTGTCCCCGCTGGAAGTGGAAAACTGCCTGAGAGAGCATGCATCCGTCATGGAATGTG
CCGTGGTAGGCGCCATGGACGAAGAGAATCTGGTGAAACCGAAGGCCTTCGTCGTCCTCAATCAGGGGTTTGCACAGTCT
CCGGAACTCGAAAAGGAATTGAAACAGTGGGTTCTCGACCGGCTGGCCAAATTCAAGTATCCGCGATGGATCGTGTTCAT
TGATTCACTCCCCAAAACTGCGACCGGGAAGATCCAGCGTTTCAAACTTCGTTAA

Upstream 100 bases:

>100_bases
AGACTCCTTAAAGGCTGATCATCATTAGAAAATATATGCGGAAGAAGTAGAGAGATGTGAATTGATTTCACATTAACCAT
TACCAAAAGGAGGAGGAAAC

Downstream 100 bases:

>100_bases
AACAGAATGGGATGACCCTTTGTCGGGGGACATCCAAAGAGTTGTGAACAGGAGCATCCCTCACAACAATTGACAATGTT
TCAAAAAAGGGCCGCCTTCC

Product: 4-hydroxybenzoate--CoA ligase / benzoate--CoA ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MAKLYPEEFYNSADWFVDRHVREGRGDNICAYTDKGNYTYRDIQKMANKMANMFKDLDIRMGDRIIMLVLDTPWFYSTFW
GAVKMGAVPVPSSTMLTPADYEYYLNDSQARTLVVSSRLLPVVNQIEELRFLKNMIVVDDDGVFSTPYQQIYASASDEFQ
TVFTSADDVAFWLYTSGTTGGPKGAVHSQSDMQYSAEAYGKHILEITEKDICYSAARLFFTYGLGNAMFFPMSVGAAAVL
NPDPPAPAHVFRLIKTYKATLFFGVPTLFGQMLITQDKIDAEKGAGADPKDHDLVSLRACPSAGEALPPDLYHKFKARYG
VEILDGPGSTEMLHIYLSNKLGDVKPGSSGKPVPGYEEKIMDEEGKNELPDGEVGNLWIKGRSSLRYYWRKRDKTAATVI
GEWVNSGDKYYKDAEGYYWPSGRADDMLKVGGIWVSPLEVENCLREHASVMECAVVGAMDEENLVKPKAFVVLNQGFAQS
PELEKELKQWVLDRLAKFKYPRWIVFIDSLPKTATGKIQRFKLR

Sequences:

>Translated_524_residues
MAKLYPEEFYNSADWFVDRHVREGRGDNICAYTDKGNYTYRDIQKMANKMANMFKDLDIRMGDRIIMLVLDTPWFYSTFW
GAVKMGAVPVPSSTMLTPADYEYYLNDSQARTLVVSSRLLPVVNQIEELRFLKNMIVVDDDGVFSTPYQQIYASASDEFQ
TVFTSADDVAFWLYTSGTTGGPKGAVHSQSDMQYSAEAYGKHILEITEKDICYSAARLFFTYGLGNAMFFPMSVGAAAVL
NPDPPAPAHVFRLIKTYKATLFFGVPTLFGQMLITQDKIDAEKGAGADPKDHDLVSLRACPSAGEALPPDLYHKFKARYG
VEILDGPGSTEMLHIYLSNKLGDVKPGSSGKPVPGYEEKIMDEEGKNELPDGEVGNLWIKGRSSLRYYWRKRDKTAATVI
GEWVNSGDKYYKDAEGYYWPSGRADDMLKVGGIWVSPLEVENCLREHASVMECAVVGAMDEENLVKPKAFVVLNQGFAQS
PELEKELKQWVLDRLAKFKYPRWIVFIDSLPKTATGKIQRFKLR
>Mature_523_residues
AKLYPEEFYNSADWFVDRHVREGRGDNICAYTDKGNYTYRDIQKMANKMANMFKDLDIRMGDRIIMLVLDTPWFYSTFWG
AVKMGAVPVPSSTMLTPADYEYYLNDSQARTLVVSSRLLPVVNQIEELRFLKNMIVVDDDGVFSTPYQQIYASASDEFQT
VFTSADDVAFWLYTSGTTGGPKGAVHSQSDMQYSAEAYGKHILEITEKDICYSAARLFFTYGLGNAMFFPMSVGAAAVLN
PDPPAPAHVFRLIKTYKATLFFGVPTLFGQMLITQDKIDAEKGAGADPKDHDLVSLRACPSAGEALPPDLYHKFKARYGV
EILDGPGSTEMLHIYLSNKLGDVKPGSSGKPVPGYEEKIMDEEGKNELPDGEVGNLWIKGRSSLRYYWRKRDKTAATVIG
EWVNSGDKYYKDAEGYYWPSGRADDMLKVGGIWVSPLEVENCLREHASVMECAVVGAMDEENLVKPKAFVVLNQGFAQSP
ELEKELKQWVLDRLAKFKYPRWIVFIDSLPKTATGKIQRFKLR

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=525, Percent_Identity=30.0952380952381, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI38505220, Length=538, Percent_Identity=32.1561338289963, Blast_Score=233, Evalue=5e-61,
Organism=Homo sapiens, GI122937307, Length=527, Percent_Identity=31.3092979127135, Blast_Score=222, Evalue=7e-58,
Organism=Homo sapiens, GI58082049, Length=500, Percent_Identity=29.2, Blast_Score=199, Evalue=7e-51,
Organism=Homo sapiens, GI157311624, Length=500, Percent_Identity=28.4, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI157311622, Length=500, Percent_Identity=28.4, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI115511026, Length=541, Percent_Identity=28.2809611829945, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI187761345, Length=535, Percent_Identity=28.2242990654206, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI187761343, Length=535, Percent_Identity=28.2242990654206, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI42544134, Length=360, Percent_Identity=24.7222222222222, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI8923896, Length=571, Percent_Identity=25.7443082311734, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI28416953, Length=521, Percent_Identity=24.3761996161228, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI253970489, Length=390, Percent_Identity=30, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI195947378, Length=416, Percent_Identity=25.7211538461538, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI13375727, Length=554, Percent_Identity=23.6462093862816, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI156151445, Length=521, Percent_Identity=23.8003838771593, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI62865631, Length=557, Percent_Identity=23.5188509874327, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI13325055, Length=557, Percent_Identity=23.5188509874327, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1790505, Length=560, Percent_Identity=26.25, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI221142682, Length=499, Percent_Identity=28.8577154308617, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1788107, Length=530, Percent_Identity=26.0377358490566, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI145693145, Length=511, Percent_Identity=28.9628180039139, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1786810, Length=543, Percent_Identity=25.7826887661142, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789201, Length=363, Percent_Identity=27.8236914600551, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786529, Length=544, Percent_Identity=22.4264705882353, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788595, Length=510, Percent_Identity=22.9411764705882, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557194, Length=529, Percent_Identity=26.0869565217391, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=539, Percent_Identity=26.1595547309833, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=24.8015873015873, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=535, Percent_Identity=25.0467289719626, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=541, Percent_Identity=23.6598890942699, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71996755, Length=533, Percent_Identity=24.577861163227, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=490, Percent_Identity=25.5102040816327, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=372, Percent_Identity=26.6129032258064, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=372, Percent_Identity=26.6129032258064, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=372, Percent_Identity=26.6129032258064, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71982997, Length=559, Percent_Identity=23.2558139534884, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71983001, Length=559, Percent_Identity=23.2558139534884, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=363, Percent_Identity=28.099173553719, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=421, Percent_Identity=28.0285035629454, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=501, Percent_Identity=22.9540918163673, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=536, Percent_Identity=25.3731343283582, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6319699, Length=515, Percent_Identity=27.1844660194175, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319264, Length=532, Percent_Identity=24.812030075188, Blast_Score=141, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24667955, Length=564, Percent_Identity=26.5957446808511, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=24.2937853107345, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI62472339, Length=533, Percent_Identity=25.891181988743, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24667959, Length=371, Percent_Identity=29.6495956873315, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI18859661, Length=519, Percent_Identity=25.4335260115607, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21355181, Length=524, Percent_Identity=23.8549618320611, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355225, Length=565, Percent_Identity=22.4778761061947, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648260, Length=515, Percent_Identity=22.9126213592233, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24653035, Length=363, Percent_Identity=26.4462809917355, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356441, Length=388, Percent_Identity=25, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19922652, Length=523, Percent_Identity=22.7533460803059, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648253, Length=391, Percent_Identity=26.0869565217391, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281365686, Length=366, Percent_Identity=25.4098360655738, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656500, Length=504, Percent_Identity=20.2380952380952, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648255, Length=391, Percent_Identity=26.3427109974425, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45550496, Length=565, Percent_Identity=21.9469026548673, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI45552801, Length=565, Percent_Identity=21.9469026548673, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20130357, Length=391, Percent_Identity=24.0409207161125, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356947, Length=357, Percent_Identity=25.4901960784314, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358303, Length=488, Percent_Identity=23.7704918032787, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI161076582, Length=366, Percent_Identity=24.0437158469945, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648257, Length=358, Percent_Identity=25.4189944134078, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 58990; Mature: 58859

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLYPEEFYNSADWFVDRHVREGRGDNICAYTDKGNYTYRDIQKMANKMANMFKDLDIR
CCCCCCHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCC
MGDRIIMLVLDTPWFYSTFWGAVKMGAVPVPSSTMLTPADYEYYLNDSQARTLVVSSRLL
CCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEECCCCCEEEECCCCCEEEEEHHHHH
PVVNQIEELRFLKNMIVVDDDGVFSTPYQQIYASASDEFQTVFTSADDVAFWLYTSGTTG
HHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCCC
GPKGAVHSQSDMQYSAEAYGKHILEITEKDICYSAARLFFTYGLGNAMFFPMSVGAAAVL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEE
NPDPPAPAHVFRLIKTYKATLFFGVPTLFGQMLITQDKIDAEKGAGADPKDHDLVSLRAC
CCCCCCHHHHHHHHHHHHHHHEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEC
PSAGEALPPDLYHKFKARYGVEILDGPGSTEMLHIYLSNKLGDVKPGSSGKPVPGYEEKI
CCCCCCCCHHHHHHHHHHCCCEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHH
MDEEGKNELPDGEVGNLWIKGRSSLRYYWRKRDKTAATVIGEWVNSGDKYYKDAEGYYWP
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEECCCCEECC
SGRADDMLKVGGIWVSPLEVENCLREHASVMECAVVGAMDEENLVKPKAFVVLNQGFAQS
CCCCCCEEEECCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCC
PELEKELKQWVLDRLAKFKYPRWIVFIDSLPKTATGKIQRFKLR
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEECC
>Mature Secondary Structure 
AKLYPEEFYNSADWFVDRHVREGRGDNICAYTDKGNYTYRDIQKMANKMANMFKDLDIR
CCCCCHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCC
MGDRIIMLVLDTPWFYSTFWGAVKMGAVPVPSSTMLTPADYEYYLNDSQARTLVVSSRLL
CCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEECCCCCEEEECCCCCEEEEEHHHHH
PVVNQIEELRFLKNMIVVDDDGVFSTPYQQIYASASDEFQTVFTSADDVAFWLYTSGTTG
HHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCCC
GPKGAVHSQSDMQYSAEAYGKHILEITEKDICYSAARLFFTYGLGNAMFFPMSVGAAAVL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEE
NPDPPAPAHVFRLIKTYKATLFFGVPTLFGQMLITQDKIDAEKGAGADPKDHDLVSLRAC
CCCCCCHHHHHHHHHHHHHHHEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEC
PSAGEALPPDLYHKFKARYGVEILDGPGSTEMLHIYLSNKLGDVKPGSSGKPVPGYEEKI
CCCCCCCCHHHHHHHHHHCCCEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHH
MDEEGKNELPDGEVGNLWIKGRSSLRYYWRKRDKTAATVIGEWVNSGDKYYKDAEGYYWP
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEECCCCEECC
SGRADDMLKVGGIWVSPLEVENCLREHASVMECAVVGAMDEENLVKPKAFVVLNQGFAQS
CCCCCCEEEECCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCC
PELEKELKQWVLDRLAKFKYPRWIVFIDSLPKTATGKIQRFKLR
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]