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The map label for this gene is hyfR [H]

Identifier: 85858601

GI number: 85858601

Start: 766633

End: 768726

Strand: Direct

Name: hyfR [H]

Synonym: SYN_02956

Alternate gene names: 85858601

Gene position: 766633-768726 (Clockwise)

Preceding gene: 85858600

Following gene: 85858602

Centisome position: 24.11

GC content: 51.81

Gene sequence:

>2094_bases
TTGCCGAACTTCCGGCATGGTAGTTTATATCCACGGGCCGCGATGTTGTGTCGAGAAAGGCCTCAAAGCGCGGAACCAGG
GGGGAGAGTGCGAACGCTTACGGATACCGGCGAACGACTGAACAGTGAGATCGCAAGGCGGAAGCGGGCCGAGCGAACCC
TGAGAGATCGCTTGCTCTTCGAGGAAATGCTTTCCGTAATTTCCGCAGGATTCATCAATTTGTCTTTCGAGCGGATTGAT
GAGGAAATCCATCATGCAATGAAGAAAGTTCTGAACTTCTTCAAAGTAGATCGGTTCGCCCTTCTTGAAACGTTACCGGA
CAGAAAATCATGGATGATCACTCATTGTGTATCCGGCACAGATGTGCCTTCCGTTCCTGTTGGTGAAAATCTCCCGGTCT
CTCTTCATCCCTGGGCCTATGAAAAGCTGATTCAGAAACGGGAAGTTCTCTCCTTTTCAAAGATAGACGATTTACCGGCT
GAGGCAGACGTTGATAAAGAAAACTGGATCGTATGGGGTATCCGATCCAACCTGAACATACCCATTAGAATCGGAAAACC
CGTTGATCATATTGTTGCCATCAATTCAGTGAAGTACGAACGTGTCTGGCCGGATGAATTGATCCCGCGACTGCGGCTTC
TGGGAGAAATTTTCGTCAATGCCCTGGAGCGAAGCAGAGCCTGGAAGGCCTTGCAGGAAAGCGAGGAACGTCTGAACCTG
GCGACTGTCGCGGCGGGAGCCGGTCTATGGATTCTGGACACAAATACGGGTCAATTCTGGGTGACGCAGATGCTCAGAAA
ATTATTTAATTTTCTTCCCGGGGAAGAATTAAGTTTTGAGCGGTATGAGAAAACAATCCATCCCGACGATCGGAAAGGAG
TCAGAGAACTTGTTCAGCGAAGCTTGAAGACGCAGAATGATCTGACCATCGAATATCGTATCCTGAAACCCGATGGGAAT
GTCTGCTGGATTATGACCCGTGGGCGTCCATATGGCGGCGCCACGGGAATTCCCCTGCGCCTGATGGGCATATGCATGGA
CATTACAGAAAGAAAGGAGATGGAAAACGAGCTCAGGAAGCAACTGGCGGAGATCAACAAACTGAAACTCCAATTGGAGA
AAGAGAATATTTATCTCCGTAATGAGATAAAGACGGAACGGGGCTTCGGCGGGATTATCGGACACAGCGATACCCTGCGA
TATGTCCTTTTCAGGGTTCAGCAGGTCGCCCCTACCGACGCTACGGTCCTTATTCTTGGCGAGACCGGTGTGGGCAAGGG
GATGGTGGCCCACGCCATCCACGAAATGAGCGCACGCAAGGATAAGACAATGGTCACTGTAAACTGTGCCGCCCTGCCGT
CAAACCTCATCGAAAGCGAACTCTTCGGGCGGGAGAAAGGGGCCTTTACAGGTGCTTACGCCCGACAGGCCGGTCGTTTT
GAGGTGGCGGACGGGGGTACGATCTTCCTGGATGAAATCGGTGAGCTCCCTCTTGAACTTCAGACAAAGCTCCTGAGGGT
GCTGCAGGACGGAGAGTTCGAGAGGCTTGGTTCCCCCAGGACGGTCAAGGTGGATGTGAGGGTGATCGCGTCGACGAGCC
GGGATCTGAAGGTTGAGATGCACAACGGGCGGTTCAGGGAGGACCTCTACTACCGGCTTAACACATTTCCCGTTACACTG
CCGCCGTTGAGGATGCGAGTCGAGGACATTCCGGAGCTTGCCAGGCATTTTATAGACAAGTATTCCCGGAAGTTAAACAA
ACAGTTTGAATCTCTTTCGAAAGATACGATGCAGATGCTTCAAGCGTATGCATGGCCCGGCAATGTGAGGGAGTTGGAAC
ATGTCATAGAAAGAGGAGTCATCACAAGTCCGGAACCTGTATTTCAGCTTGTTGAACAGCTTGAACAGGGGCCTGGCATG
GAGGCTGATAAGCCGATTAAGGGGTTTGAAACCATGGCGAGGGAGCACATCCTGCAGGTCCTTCACAAAACAGGGTGGAA
AATCGAGGGTAAAGGCGGGGCTGCCGCCATCCTCGGTCTCAATCCAAGCACGCTTCGGTTCCGGCTTAAAAAGCTGGGGA
TCAAACGACCCTGA

Upstream 100 bases:

>100_bases
TCGTTCATTGACGATGCGCATGGAATCAAGAGGGAGTCCGGGAAATAAGCCACTCATTTTTGCGCGGCACTTAAGGTCTT
GATAATCAAGGGAAGTAATT

Downstream 100 bases:

>100_bases
AAAGATGAATTTCCTCAATACGATTCCTCAAAATCTTGCAGATCCGCAATATGTTGAGGGTATTCCCATATCCATTCACC
GGGACTGGCCAGTGTCATTA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MPNFRHGSLYPRAAMLCRERPQSAEPGGRVRTLTDTGERLNSEIARRKRAERTLRDRLLFEEMLSVISAGFINLSFERID
EEIHHAMKKVLNFFKVDRFALLETLPDRKSWMITHCVSGTDVPSVPVGENLPVSLHPWAYEKLIQKREVLSFSKIDDLPA
EADVDKENWIVWGIRSNLNIPIRIGKPVDHIVAINSVKYERVWPDELIPRLRLLGEIFVNALERSRAWKALQESEERLNL
ATVAAGAGLWILDTNTGQFWVTQMLRKLFNFLPGEELSFERYEKTIHPDDRKGVRELVQRSLKTQNDLTIEYRILKPDGN
VCWIMTRGRPYGGATGIPLRLMGICMDITERKEMENELRKQLAEINKLKLQLEKENIYLRNEIKTERGFGGIIGHSDTLR
YVLFRVQQVAPTDATVLILGETGVGKGMVAHAIHEMSARKDKTMVTVNCAALPSNLIESELFGREKGAFTGAYARQAGRF
EVADGGTIFLDEIGELPLELQTKLLRVLQDGEFERLGSPRTVKVDVRVIASTSRDLKVEMHNGRFREDLYYRLNTFPVTL
PPLRMRVEDIPELARHFIDKYSRKLNKQFESLSKDTMQMLQAYAWPGNVRELEHVIERGVITSPEPVFQLVEQLEQGPGM
EADKPIKGFETMAREHILQVLHKTGWKIEGKGGAAAILGLNPSTLRFRLKKLGIKRP

Sequences:

>Translated_697_residues
MPNFRHGSLYPRAAMLCRERPQSAEPGGRVRTLTDTGERLNSEIARRKRAERTLRDRLLFEEMLSVISAGFINLSFERID
EEIHHAMKKVLNFFKVDRFALLETLPDRKSWMITHCVSGTDVPSVPVGENLPVSLHPWAYEKLIQKREVLSFSKIDDLPA
EADVDKENWIVWGIRSNLNIPIRIGKPVDHIVAINSVKYERVWPDELIPRLRLLGEIFVNALERSRAWKALQESEERLNL
ATVAAGAGLWILDTNTGQFWVTQMLRKLFNFLPGEELSFERYEKTIHPDDRKGVRELVQRSLKTQNDLTIEYRILKPDGN
VCWIMTRGRPYGGATGIPLRLMGICMDITERKEMENELRKQLAEINKLKLQLEKENIYLRNEIKTERGFGGIIGHSDTLR
YVLFRVQQVAPTDATVLILGETGVGKGMVAHAIHEMSARKDKTMVTVNCAALPSNLIESELFGREKGAFTGAYARQAGRF
EVADGGTIFLDEIGELPLELQTKLLRVLQDGEFERLGSPRTVKVDVRVIASTSRDLKVEMHNGRFREDLYYRLNTFPVTL
PPLRMRVEDIPELARHFIDKYSRKLNKQFESLSKDTMQMLQAYAWPGNVRELEHVIERGVITSPEPVFQLVEQLEQGPGM
EADKPIKGFETMAREHILQVLHKTGWKIEGKGGAAAILGLNPSTLRFRLKKLGIKRP
>Mature_696_residues
PNFRHGSLYPRAAMLCRERPQSAEPGGRVRTLTDTGERLNSEIARRKRAERTLRDRLLFEEMLSVISAGFINLSFERIDE
EIHHAMKKVLNFFKVDRFALLETLPDRKSWMITHCVSGTDVPSVPVGENLPVSLHPWAYEKLIQKREVLSFSKIDDLPAE
ADVDKENWIVWGIRSNLNIPIRIGKPVDHIVAINSVKYERVWPDELIPRLRLLGEIFVNALERSRAWKALQESEERLNLA
TVAAGAGLWILDTNTGQFWVTQMLRKLFNFLPGEELSFERYEKTIHPDDRKGVRELVQRSLKTQNDLTIEYRILKPDGNV
CWIMTRGRPYGGATGIPLRLMGICMDITERKEMENELRKQLAEINKLKLQLEKENIYLRNEIKTERGFGGIIGHSDTLRY
VLFRVQQVAPTDATVLILGETGVGKGMVAHAIHEMSARKDKTMVTVNCAALPSNLIESELFGREKGAFTGAYARQAGRFE
VADGGTIFLDEIGELPLELQTKLLRVLQDGEFERLGSPRTVKVDVRVIASTSRDLKVEMHNGRFREDLYYRLNTFPVTLP
PLRMRVEDIPELARHFIDKYSRKLNKQFESLSKDTMQMLQAYAWPGNVRELEHVIERGVITSPEPVFQLVEQLEQGPGME
ADKPIKGFETMAREHILQVLHKTGWKIEGKGGAAAILGLNPSTLRFRLKKLGIKRP

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=348, Percent_Identity=47.4137931034483, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1789087, Length=349, Percent_Identity=48.4240687679083, Blast_Score=309, Evalue=4e-85,
Organism=Escherichia coli, GI1790437, Length=318, Percent_Identity=43.7106918238994, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI1788550, Length=341, Percent_Identity=42.2287390029325, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI87082152, Length=232, Percent_Identity=51.2931034482759, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1790299, Length=224, Percent_Identity=52.2321428571429, Blast_Score=234, Evalue=2e-62,
Organism=Escherichia coli, GI1788905, Length=254, Percent_Identity=46.0629921259843, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1789233, Length=234, Percent_Identity=43.1623931623932, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1786524, Length=358, Percent_Identity=39.3854748603352, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=38.2445141065831, Blast_Score=203, Evalue=4e-53,
Organism=Escherichia coli, GI1787583, Length=277, Percent_Identity=38.6281588447653, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI87081858, Length=306, Percent_Identity=34.3137254901961, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1789828, Length=303, Percent_Identity=33.003300330033, Blast_Score=134, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 79668; Mature: 79537

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNFRHGSLYPRAAMLCRERPQSAEPGGRVRTLTDTGERLNSEIARRKRAERTLRDRLLF
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EEMLSVISAGFINLSFERIDEEIHHAMKKVLNFFKVDRFALLETLPDRKSWMITHCVSGT
HHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
DVPSVPVGENLPVSLHPWAYEKLIQKREVLSFSKIDDLPAEADVDKENWIVWGIRSNLNI
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEE
PIRIGKPVDHIVAINSVKYERVWPDELIPRLRLLGEIFVNALERSRAWKALQESEERLNL
EEEECCCHHHEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATVAAGAGLWILDTNTGQFWVTQMLRKLFNFLPGEELSFERYEKTIHPDDRKGVRELVQR
HEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHH
SLKTQNDLTIEYRILKPDGNVCWIMTRGRPYGGATGIPLRLMGICMDITERKEMENELRK
HHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QLAEINKLKLQLEKENIYLRNEIKTERGFGGIIGHSDTLRYVLFRVQQVAPTDATVLILG
HHHHHHHHEEEEECCCEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
ETGVGKGMVAHAIHEMSARKDKTMVTVNCAALPSNLIESELFGREKGAFTGAYARQAGRF
CCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHCCCCE
EVADGGTIFLDEIGELPLELQTKLLRVLQDGEFERLGSPRTVKVDVRVIASTSRDLKVEM
EECCCCEEEECHHCCCCHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEEEECCCCCEEEEE
HNGRFREDLYYRLNTFPVTLPPLRMRVEDIPELARHFIDKYSRKLNKQFESLSKDTMQML
CCCCCHHHHHEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAYAWPGNVRELEHVIERGVITSPEPVFQLVEQLEQGPGMEADKPIKGFETMAREHILQV
HHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LHKTGWKIEGKGGAAAILGLNPSTLRFRLKKLGIKRP
HHHCCCEEECCCCEEEEEECCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PNFRHGSLYPRAAMLCRERPQSAEPGGRVRTLTDTGERLNSEIARRKRAERTLRDRLLF
CCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EEMLSVISAGFINLSFERIDEEIHHAMKKVLNFFKVDRFALLETLPDRKSWMITHCVSGT
HHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
DVPSVPVGENLPVSLHPWAYEKLIQKREVLSFSKIDDLPAEADVDKENWIVWGIRSNLNI
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEE
PIRIGKPVDHIVAINSVKYERVWPDELIPRLRLLGEIFVNALERSRAWKALQESEERLNL
EEEECCCHHHEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATVAAGAGLWILDTNTGQFWVTQMLRKLFNFLPGEELSFERYEKTIHPDDRKGVRELVQR
HEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHH
SLKTQNDLTIEYRILKPDGNVCWIMTRGRPYGGATGIPLRLMGICMDITERKEMENELRK
HHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QLAEINKLKLQLEKENIYLRNEIKTERGFGGIIGHSDTLRYVLFRVQQVAPTDATVLILG
HHHHHHHHEEEEECCCEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
ETGVGKGMVAHAIHEMSARKDKTMVTVNCAALPSNLIESELFGREKGAFTGAYARQAGRF
CCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHCCCCE
EVADGGTIFLDEIGELPLELQTKLLRVLQDGEFERLGSPRTVKVDVRVIASTSRDLKVEM
EECCCCEEEECHHCCCCHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEEEECCCCCEEEEE
HNGRFREDLYYRLNTFPVTLPPLRMRVEDIPELARHFIDKYSRKLNKQFESLSKDTMQML
CCCCCHHHHHEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAYAWPGNVRELEHVIERGVITSPEPVFQLVEQLEQGPGMEADKPIKGFETMAREHILQV
HHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LHKTGWKIEGKGGAAAILGLNPSTLRFRLKKLGIKRP
HHHCCCEEECCCCEEEEEECCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]