Definition Syntrophus aciditrophicus SB chromosome, complete genome.
Accession NC_007759
Length 3,179,300

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The map label for this gene is tyrS

Identifier: 85858578

GI number: 85858578

Start: 736415

End: 737701

Strand: Direct

Name: tyrS

Synonym: SYN_02977

Alternate gene names: 85858578

Gene position: 736415-737701 (Clockwise)

Preceding gene: 85858577

Following gene: 85858579

Centisome position: 23.16

GC content: 42.04

Gene sequence:

>1287_bases
TTGATTTTGAAAAACGTATACGATGTTTTCATGGAAAGAGGATTTATAGAACAGGTCACTGACGAAAATGCAGTAAGAAA
AGCTCTTGAAGCACCGCTTGCCTGTTACATTGGTTTTGATCCCACAGCGAGAAGCCTGCATATTGGAAGTCTGGTTCCCA
TTATGGCTCTTATTCATTTACAGCGACATGGTCATACGTCCATTGCCCTGGTTGGGGGAGGGACGGCTCTGATCGGAGAT
CCAAGTGGGAAAACGGAAATGCGTCAGATTCTGACAAGGGAAAAGATAGAACTCAACGCAACATGCATGCGAAGGCAATT
TGCCAGATATTTGAGCTTTGAAGATAAAAAAGCGATGATGATCAATAATGCGGATTGGTTGACGAAACTGAATTATATTT
CGTTTTTGCGCGATATCGGGCGTCATTTCAGTGTAAACAAGATGCTTGCCGCCGAAAGTTATAAGATGCGCTTAGAGAAG
GGATTAAATTTTATAGAATTTAATTATATGCTACTGCAAGCATATGATTTTTTATATCTTTTCCAAAATCATAATTGTGT
GATGCAAATGGGAGGCAATGATCAGTGGGGCAATATGTTGGCCGGTGTCGATCTCATCCGGAGGGTTGAAGGAAAGGTTG
CACACAGCATGACATTTCCACTTCTAACGACGGCGACAGGGCAAAAAATGGGAAAAACTGAAAAGGGAGCTGTCTGGCTT
GATCGGGAATTAACCTCACCATATGAATACTATCAATATTGGATTAATACCGGCGATATTGATGTTGGGAAATTTCTTGC
TTTGTTTACCTTCCTGCCTATGGAAGAAATACATCAGGTAAAATCTTTGAGTGATAAAGAGTTGAACATGGCAAAGGCAA
TACTGGCTTTTGAGGCCACCAAGATTACGCATGGCGAGGATGCCGCACTTGCGGCCTGGAATGCATCTGCGGTGGCATTT
GGAGTAAAATTAATCGACACATCACTCATGCCTTCAAGTACGATTCCGCGCGGTCAGCTTTCGCAGGACGCCTCTGCCAT
TCCATTCATTAAAAAATCGTGGAATGAGCTCGCTAAAGGCATCCCGGCATATGAAATTATGCATGAATGTGGTTTGTGCA
GTTCGAAAAGTGAGGCCAGAAGATTGATTGCTCAGGGAGGCGGTTATGTGAATGAAAATCCGATTTTCGCATTCGACGAA
TTAATAACCACTGAGCATTTGGACCGTAGTGGGCAAATAAAGTTAAGAAAAGGCAAGAAAAAATATATGATTATCAAAGT
TGAATAA

Upstream 100 bases:

>100_bases
ATCTGTCTTGATAATTGACCCGGGTAGTTTTAAGTGGCGATCAGCAAAGTAAAAAATACATTATAAAATAAAGCTTAAGA
TGGGACTGATTTAGGGGACG

Downstream 100 bases:

>100_bases
AAATTGTAGAAAAAGTCCTTGACAGATAAAGTTTGAAAAGGTACAAAGCGCCTCCTTTATTTCACAGATGCCTCTGCACT
ACAAAACGGCAAAGGTTGTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MILKNVYDVFMERGFIEQVTDENAVRKALEAPLACYIGFDPTARSLHIGSLVPIMALIHLQRHGHTSIALVGGGTALIGD
PSGKTEMRQILTREKIELNATCMRRQFARYLSFEDKKAMMINNADWLTKLNYISFLRDIGRHFSVNKMLAAESYKMRLEK
GLNFIEFNYMLLQAYDFLYLFQNHNCVMQMGGNDQWGNMLAGVDLIRRVEGKVAHSMTFPLLTTATGQKMGKTEKGAVWL
DRELTSPYEYYQYWINTGDIDVGKFLALFTFLPMEEIHQVKSLSDKELNMAKAILAFEATKITHGEDAALAAWNASAVAF
GVKLIDTSLMPSSTIPRGQLSQDASAIPFIKKSWNELAKGIPAYEIMHECGLCSSKSEARRLIAQGGGYVNENPIFAFDE
LITTEHLDRSGQIKLRKGKKKYMIIKVE

Sequences:

>Translated_428_residues
MILKNVYDVFMERGFIEQVTDENAVRKALEAPLACYIGFDPTARSLHIGSLVPIMALIHLQRHGHTSIALVGGGTALIGD
PSGKTEMRQILTREKIELNATCMRRQFARYLSFEDKKAMMINNADWLTKLNYISFLRDIGRHFSVNKMLAAESYKMRLEK
GLNFIEFNYMLLQAYDFLYLFQNHNCVMQMGGNDQWGNMLAGVDLIRRVEGKVAHSMTFPLLTTATGQKMGKTEKGAVWL
DRELTSPYEYYQYWINTGDIDVGKFLALFTFLPMEEIHQVKSLSDKELNMAKAILAFEATKITHGEDAALAAWNASAVAF
GVKLIDTSLMPSSTIPRGQLSQDASAIPFIKKSWNELAKGIPAYEIMHECGLCSSKSEARRLIAQGGGYVNENPIFAFDE
LITTEHLDRSGQIKLRKGKKKYMIIKVE
>Mature_428_residues
MILKNVYDVFMERGFIEQVTDENAVRKALEAPLACYIGFDPTARSLHIGSLVPIMALIHLQRHGHTSIALVGGGTALIGD
PSGKTEMRQILTREKIELNATCMRRQFARYLSFEDKKAMMINNADWLTKLNYISFLRDIGRHFSVNKMLAAESYKMRLEK
GLNFIEFNYMLLQAYDFLYLFQNHNCVMQMGGNDQWGNMLAGVDLIRRVEGKVAHSMTFPLLTTATGQKMGKTEKGAVWL
DRELTSPYEYYQYWINTGDIDVGKFLALFTFLPMEEIHQVKSLSDKELNMAKAILAFEATKITHGEDAALAAWNASAVAF
GVKLIDTSLMPSSTIPRGQLSQDASAIPFIKKSWNELAKGIPAYEIMHECGLCSSKSEARRLIAQGGGYVNENPIFAFDE
LITTEHLDRSGQIKLRKGKKKYMIIKVE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=409, Percent_Identity=34.718826405868, Blast_Score=238, Evalue=9e-63,
Organism=Escherichia coli, GI1787925, Length=442, Percent_Identity=41.4027149321267, Blast_Score=319, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25145802, Length=352, Percent_Identity=36.3636363636364, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI25145805, Length=226, Percent_Identity=35.8407079646018, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325160, Length=463, Percent_Identity=33.9092872570194, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI20130383, Length=449, Percent_Identity=36.0801781737194, Blast_Score=268, Evalue=4e-72,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_SYNAS (Q2LR98)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_460780.1
- ProteinModelPortal:   Q2LR98
- SMR:   Q2LR98
- STRING:   Q2LR98
- GeneID:   3885323
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02977
- NMPDR:   fig|56780.10.peg.724
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- PhylomeDB:   Q2LR98
- ProtClustDB:   PRK05912
- BioCyc:   SACI56780:SYN_02977-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 48283; Mature: 48283

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 36-36 BINDING 169-169 BINDING 173-173 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MILKNVYDVFMERGFIEQVTDENAVRKALEAPLACYIGFDPTARSLHIGSLVPIMALIHL
CCHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCEEEEECCCCCCCCEECHHHHHHHHHHHH
QRHGHTSIALVGGGTALIGDPSGKTEMRQILTREKIELNATCMRRQFARYLSFEDKKAMM
HHCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
INNADWLTKLNYISFLRDIGRHFSVNKMLAAESYKMRLEKGLNFIEFNYMLLQAYDFLYL
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHEEEE
FQNHNCVMQMGGNDQWGNMLAGVDLIRRVEGKVAHSMTFPLLTTATGQKMGKTEKGAVWL
EECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEECCCHHCCCCCCCCEEE
DRELTSPYEYYQYWINTGDIDVGKFLALFTFLPMEEIHQVKSLSDKELNMAKAILAFEAT
ECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
KITHGEDAALAAWNASAVAFGVKLIDTSLMPSSTIPRGQLSQDASAIPFIKKSWNELAKG
EECCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
IPAYEIMHECGLCSSKSEARRLIAQGGGYVNENPIFAFDELITTEHLDRSGQIKLRKGKK
CCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCC
KYMIIKVE
EEEEEEEC
>Mature Secondary Structure
MILKNVYDVFMERGFIEQVTDENAVRKALEAPLACYIGFDPTARSLHIGSLVPIMALIHL
CCHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCEEEEECCCCCCCCEECHHHHHHHHHHHH
QRHGHTSIALVGGGTALIGDPSGKTEMRQILTREKIELNATCMRRQFARYLSFEDKKAMM
HHCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
INNADWLTKLNYISFLRDIGRHFSVNKMLAAESYKMRLEKGLNFIEFNYMLLQAYDFLYL
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHEEEE
FQNHNCVMQMGGNDQWGNMLAGVDLIRRVEGKVAHSMTFPLLTTATGQKMGKTEKGAVWL
EECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEECCCHHCCCCCCCCEEE
DRELTSPYEYYQYWINTGDIDVGKFLALFTFLPMEEIHQVKSLSDKELNMAKAILAFEAT
ECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
KITHGEDAALAAWNASAVAFGVKLIDTSLMPSSTIPRGQLSQDASAIPFIKKSWNELAKG
EECCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
IPAYEIMHECGLCSSKSEARRLIAQGGGYVNENPIFAFDELITTEHLDRSGQIKLRKGKK
CCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCC
KYMIIKVE
EEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA