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The map label for this gene is xthA [H]

Identifier: 85858539

GI number: 85858539

Start: 696777

End: 697580

Strand: Direct

Name: xthA [H]

Synonym: SYN_00429

Alternate gene names: 85858539

Gene position: 696777-697580 (Clockwise)

Preceding gene: 85858538

Following gene: 85858541

Centisome position: 21.92

GC content: 52.74

Gene sequence:

>804_bases
GTGACCGCAAAGACTATGGCTCGATTCAAGATTGCCACATACAATGTCAATTCCATCCGCTCCCGTCTTCATATCATTCT
TTCCTGGCTTGAGGAAAACAGGCCCGATGTGTTCTGCATGCAGGAAACGAAAGTGGCGGATGCCCGGTTTCCCGCTGACG
CTTTCACGGTTGCAGGCTATCATGTCGTTTTCCGGGGAATGAAGCAGTACAATGGAGTTGCAATCGCTTCTCTGGAAAAA
CCGGAAAACGTCACATATGGATTAGGCGGAGAGGCAGACGACGCGGATGAGGATCGCTTGATTGCCGCCGTTTATTCCGG
TGTTCCGGTGATCAACACCTATGTTCCTCAGGGCCGCAATCGGGAAAACCCCCAGTTTGCCTACAAACTTCAATGGTATG
CCCGCTTTCGGGAATATCTGGTTCAAAAGTGGTCATCAGATGCCCCCCTCGTCTGGTGCGGTGATCTCAATGTCGCGCCG
GAACAGATCGATGTCCATGATCCCAAACGTCTTCTCGGGCATGTCTGTTTCAACCCCGAGGTATGGGATGCCTTTGACCG
GGTCAAGTCCTGGGGACTGGAAGACCTGTTCCGCAAGCATCACCCCGGAGAAGTGGAATGTTACACCTTTTTTGATTACC
GGGTTCCCCGATCGGTGGAGAGAAAGCTGGGGTGGCGGATTGATCACATTCTTTCCACCCCCGCCTTGGCCGCGCACTGC
GATTCATGCAACATCGATATGAACCCGCGGATGGCCGAGAAACCATCGGATCATACAATCCTGGTTGCGGAGTTCACTAT
TTGA

Upstream 100 bases:

>100_bases
CGGGTGTTGAAGGGTCGAGGTATGCACATGTCGACTCTGTCGGTATTCGCGACATCATCATCCGGGACCCTGATCCGCGT
CGGCTGTTGGCAGCAGGGAG

Downstream 100 bases:

>100_bases
AAAAAGTAATCTGCAGATGAAAGAGGAGGGATAACGAAACACGTGAAGCAGATCATGGACACACTGAAGAAGAATGAAGA
AATCGCCAGAAAATTTCTGA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTAKTMARFKIATYNVNSIRSRLHIILSWLEENRPDVFCMQETKVADARFPADAFTVAGYHVVFRGMKQYNGVAIASLEK
PENVTYGLGGEADDADEDRLIAAVYSGVPVINTYVPQGRNRENPQFAYKLQWYARFREYLVQKWSSDAPLVWCGDLNVAP
EQIDVHDPKRLLGHVCFNPEVWDAFDRVKSWGLEDLFRKHHPGEVECYTFFDYRVPRSVERKLGWRIDHILSTPALAAHC
DSCNIDMNPRMAEKPSDHTILVAEFTI

Sequences:

>Translated_267_residues
MTAKTMARFKIATYNVNSIRSRLHIILSWLEENRPDVFCMQETKVADARFPADAFTVAGYHVVFRGMKQYNGVAIASLEK
PENVTYGLGGEADDADEDRLIAAVYSGVPVINTYVPQGRNRENPQFAYKLQWYARFREYLVQKWSSDAPLVWCGDLNVAP
EQIDVHDPKRLLGHVCFNPEVWDAFDRVKSWGLEDLFRKHHPGEVECYTFFDYRVPRSVERKLGWRIDHILSTPALAAHC
DSCNIDMNPRMAEKPSDHTILVAEFTI
>Mature_266_residues
TAKTMARFKIATYNVNSIRSRLHIILSWLEENRPDVFCMQETKVADARFPADAFTVAGYHVVFRGMKQYNGVAIASLEKP
ENVTYGLGGEADDADEDRLIAAVYSGVPVINTYVPQGRNRENPQFAYKLQWYARFREYLVQKWSSDAPLVWCGDLNVAPE
QIDVHDPKRLLGHVCFNPEVWDAFDRVKSWGLEDLFRKHHPGEVECYTFFDYRVPRSVERKLGWRIDHILSTPALAAHCD
SCNIDMNPRMAEKPSDHTILVAEFTI

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=243, Percent_Identity=34.156378600823, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI18375503, Length=243, Percent_Identity=34.156378600823, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI18375501, Length=243, Percent_Identity=34.156378600823, Blast_Score=129, Evalue=2e-30,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=36.3295880149813, Blast_Score=166, Evalue=1e-42,
Organism=Drosophila melanogaster, GI221330655, Length=228, Percent_Identity=36.8421052631579, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17136678, Length=228, Percent_Identity=36.8421052631579, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30673; Mature: 30542

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKTMARFKIATYNVNSIRSRLHIILSWLEENRPDVFCMQETKVADARFPADAFTVAGY
CCCCCEEEEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHH
HVVFRGMKQYNGVAIASLEKPENVTYGLGGEADDADEDRLIAAVYSGVPVINTYVPQGRN
HHHHHHHHHHCCEEEEECCCCCCEEEECCCCCCCCCCCCEEEEHHCCCCEEEEECCCCCC
RENPQFAYKLQWYARFREYLVQKWSSDAPLVWCGDLNVAPEQIDVHDPKRLLGHVCFNPE
CCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCH
VWDAFDRVKSWGLEDLFRKHHPGEVECYTFFDYRVPRSVERKLGWRIDHILSTPALAAHC
HHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHCCCHHHHHCCCHHHHCC
DSCNIDMNPRMAEKPSDHTILVAEFTI
CCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TAKTMARFKIATYNVNSIRSRLHIILSWLEENRPDVFCMQETKVADARFPADAFTVAGY
CCCCEEEEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHH
HVVFRGMKQYNGVAIASLEKPENVTYGLGGEADDADEDRLIAAVYSGVPVINTYVPQGRN
HHHHHHHHHHCCEEEEECCCCCCEEEECCCCCCCCCCCCEEEEHHCCCCEEEEECCCCCC
RENPQFAYKLQWYARFREYLVQKWSSDAPLVWCGDLNVAPEQIDVHDPKRLLGHVCFNPE
CCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCH
VWDAFDRVKSWGLEDLFRKHHPGEVECYTFFDYRVPRSVERKLGWRIDHILSTPALAAHC
HHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHCCCHHHHHCCCHHHHCC
DSCNIDMNPRMAEKPSDHTILVAEFTI
CCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]