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The map label for this gene is murB

Identifier: 85858528

GI number: 85858528

Start: 681976

End: 682914

Strand: Direct

Name: murB

Synonym: SYN_01748

Alternate gene names: 85858528

Gene position: 681976-682914 (Clockwise)

Preceding gene: 85858527

Following gene: 85858529

Centisome position: 21.45

GC content: 53.57

Gene sequence:

>939_bases
TTGAGGATTCCCGTGCTGCATGATTTTTTTATCAAAGAACAATTGAAGTCCTGTGTTTCGGGGGCTGTGCTTTTCGATGA
ACCTCTGGATCGTTATACCTCCATGGGTGTGGGCGGTCCGGCCGATGCACTGGTGGTTCCGCAGAGTATGGAGGAACTGG
TGCAGCTGGTTCGTTTTCTGCGGAAAGAGAACATCCCCTTCCTGACTCTGGGAAACGGAACCAATCTGATTGTCAGAGAC
GGAGGCTGCCGGGGAGTGGTTGTCGCTCTGCGGGGGCTGCAAAAACTGTCCTGGGCTTCAGACCCGGAAGGAAAGATCCG
GGTGCAGGCGGAAGCGGGTGTCCCTCTGGCTTCGATCGTTCAGCTCTGCATCAAAGAATCTCTGGCTGGTCTGGAATTCT
GCACTGGCATCCCCGGCAGTGTAGGCGGGGCGGTTCGGATGAACGCCGGAGCATTCGGGAGAGAAATGAAAGACGTGGTA
ACAGCGATTACCGTGCTGAATGAGCATTTGGAGTTGGAGACGCTTTCCCGCCGGGAGCTTTCCTTTGAATACCGTCGTTT
GAATCTTTCCGATGAAGCGGTGATTGTCTGTGCCGAGTTTGCTCTGTGTCCAGGAGAGCGGGAATCGATATCCGCTGAAA
TCAGTGAAATTCTGGCCTTGCGGAAAAGTAAGCATCCTCTTAACTTTCGAAACGCGGGCTCCATCTTTAAAAATCCTCGC
AATCTGCCCGCGGGTCAGTTGATCGAGGAAACCGGCCTCAAGGGGACCCGTCGAGGGGATGCCATGATTTCAGAGAAGCA
TGGCAATTTCATCGTCAATCTGGGGCATGCCCGGGCCGCGGATGTGGTTGATCTGATTGAGGAGATTAAAGGTCGGGTTG
AGAATTGCCGGGCCATACAACTGGAAGCTGAGGTTCATATTGTCGGAGAAGATGGATGA

Upstream 100 bases:

>100_bases
TATTTGATGACCGTGGTGCAGCCGGGAGATACAGTGATCACACAGGGGGCGGGAAGTGTCAATTCGGTGGGCGTTTCTCT
CTTGAGCCGACTGAAGGAGG

Downstream 100 bases:

>100_bases
AAAGAGCTTTCACAAGAAAGCTGGAGGCAAAAAAATATCGATTGAAGCGTCGCAGCAGGACGATATTCGTCGATATCTTC
CGATCAATTCTCCTCATTGT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRIPVLHDFFIKEQLKSCVSGAVLFDEPLDRYTSMGVGGPADALVVPQSMEELVQLVRFLRKENIPFLTLGNGTNLIVRD
GGCRGVVVALRGLQKLSWASDPEGKIRVQAEAGVPLASIVQLCIKESLAGLEFCTGIPGSVGGAVRMNAGAFGREMKDVV
TAITVLNEHLELETLSRRELSFEYRRLNLSDEAVIVCAEFALCPGERESISAEISEILALRKSKHPLNFRNAGSIFKNPR
NLPAGQLIEETGLKGTRRGDAMISEKHGNFIVNLGHARAADVVDLIEEIKGRVENCRAIQLEAEVHIVGEDG

Sequences:

>Translated_312_residues
MRIPVLHDFFIKEQLKSCVSGAVLFDEPLDRYTSMGVGGPADALVVPQSMEELVQLVRFLRKENIPFLTLGNGTNLIVRD
GGCRGVVVALRGLQKLSWASDPEGKIRVQAEAGVPLASIVQLCIKESLAGLEFCTGIPGSVGGAVRMNAGAFGREMKDVV
TAITVLNEHLELETLSRRELSFEYRRLNLSDEAVIVCAEFALCPGERESISAEISEILALRKSKHPLNFRNAGSIFKNPR
NLPAGQLIEETGLKGTRRGDAMISEKHGNFIVNLGHARAADVVDLIEEIKGRVENCRAIQLEAEVHIVGEDG
>Mature_312_residues
MRIPVLHDFFIKEQLKSCVSGAVLFDEPLDRYTSMGVGGPADALVVPQSMEELVQLVRFLRKENIPFLTLGNGTNLIVRD
GGCRGVVVALRGLQKLSWASDPEGKIRVQAEAGVPLASIVQLCIKESLAGLEFCTGIPGSVGGAVRMNAGAFGREMKDVV
TAITVLNEHLELETLSRRELSFEYRRLNLSDEAVIVCAEFALCPGERESISAEISEILALRKSKHPLNFRNAGSIFKNPR
NLPAGQLIEETGLKGTRRGDAMISEKHGNFIVNLGHARAADVVDLIEEIKGRVENCRAIQLEAEVHIVGEDG

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=342, Percent_Identity=24.8538011695906, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_SYNAS (Q2LR56)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_460730.1
- ProteinModelPortal:   Q2LR56
- SMR:   Q2LR56
- STRING:   Q2LR56
- GeneID:   3885155
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_01748
- NMPDR:   fig|56780.10.peg.675
- eggNOG:   COG0812
- HOGENOM:   HBG686573
- OMA:   SKKHAGF
- PhylomeDB:   Q2LR56
- ProtClustDB:   PRK13905
- BioCyc:   SACI56780:SYN_01748-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 33966; Mature: 33966

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 185-185 ACT_SITE 234-234 ACT_SITE 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPVLHDFFIKEQLKSCVSGAVLFDEPLDRYTSMGVGGPADALVVPQSMEELVQLVRFL
CCCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHH
RKENIPFLTLGNGTNLIVRDGGCRGVVVALRGLQKLSWASDPEGKIRVQAEAGVPLASIV
HHCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHH
QLCIKESLAGLEFCTGIPGSVGGAVRMNAGAFGREMKDVVTAITVLNEHLELETLSRREL
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFEYRRLNLSDEAVIVCAEFALCPGERESISAEISEILALRKSKHPLNFRNAGSIFKNPR
CCHHEEECCCCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCC
NLPAGQLIEETGLKGTRRGDAMISEKHGNFIVNLGHARAADVVDLIEEIKGRVENCRAIQ
CCCHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEE
LEAEVHIVGEDG
EEEEEEEEECCC
>Mature Secondary Structure
MRIPVLHDFFIKEQLKSCVSGAVLFDEPLDRYTSMGVGGPADALVVPQSMEELVQLVRFL
CCCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHH
RKENIPFLTLGNGTNLIVRDGGCRGVVVALRGLQKLSWASDPEGKIRVQAEAGVPLASIV
HHCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHH
QLCIKESLAGLEFCTGIPGSVGGAVRMNAGAFGREMKDVVTAITVLNEHLELETLSRREL
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFEYRRLNLSDEAVIVCAEFALCPGERESISAEISEILALRKSKHPLNFRNAGSIFKNPR
CCHHEEECCCCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCC
NLPAGQLIEETGLKGTRRGDAMISEKHGNFIVNLGHARAADVVDLIEEIKGRVENCRAIQ
CCCHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEE
LEAEVHIVGEDG
EEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA