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The map label for this gene is murD

Identifier: 85858524

GI number: 85858524

Start: 676857

End: 678218

Strand: Direct

Name: murD

Synonym: SYN_01744

Alternate gene names: 85858524

Gene position: 676857-678218 (Clockwise)

Preceding gene: 85858523

Following gene: 85858525

Centisome position: 21.29

GC content: 53.89

Gene sequence:

>1362_bases
ATGTTCTTCATGGATATATCAGGACAGAGAATGCTGGTAATCGGCTGGGGAAAAACGGGTGTCGCCTCCGCGCGGTTTCT
GATTTCTCGTGGCGCCCGGGTCGTCGTGGCGGATGAAAAAAATCTTTCCCTGGAAAATGACGTACTGTCCAAACTTGGCG
AAGATCATGGTCAAAGCGTGGAACTGGCGGATTATGGAATATCGGCGCTGGCCCAGGTCGATGCCGTTGTGCCTTCGCCG
GGCGTGCCGCCGTTTCATCCCGTTCTGAAGGAAGCCGTCAAGAGATGTATCCCCGTTATCAGCGAACTCGAACTGGCCTG
CCGTTATCTCCAGACGCCCATGATCGCCATTACCGGAACGAATGGAAAGACGACCACGACGAGCCTTATCGGAGAAATAC
TGAGCGGAAGCGGCAGAAAGGTCTTCGTGGGTGGAAATATCGGGACGCCGCTGGTGGAGTACGTGACCGGGCCGCAGACA
GCCGACTGCGCGGTCGTCGAGATCAGCAGCTTCCAGCTCCAATGGGTGGATCAGTTTCACGCCTTTGTGTCCATTCTACT
GAATACGACCTGCGATCATGTAAATTATCACGGTTCGTTCGAGGCATACCGGGCCGTCAAGGAAAGAATTTTCAACAATC
AGGGAAAACAGGATGTGGCCATCCTGAATGCCGACGAACCGGATTCGGCTGTGCTGGCAGAATCTCTGTCATCACCGGTC
TTTTTTTTCAGCACCACAAAGATGGTTGAACGCGGTCTCTATCAGGAAAAGGATCGATTGATCTGTCTCGACGGAGAGGG
AAAGCAGGAGTTCTATCCTTTGAGTATGATCCGTTTGCCGGGGGCTCACAACGTCGAGAATGTCATGGCTGCCATTTTAG
CCTCCCGGGCCTGCGGCTGCAGTCCGGAAAACGTGATCAATGCGATATCCGGCTTTTCAGGTATCGCCCACCGCATTGAA
TTTACAAGGGAGATTGGCGGTGTGAAGTTTTACGACGATTCCAAGGGAACCAACGTCGGGGCCGTCAAACGGGCCATAGA
AACCTTTTCGGACCCGATCATTCTCCTGATGGGCGGAAGGGACAAGGATGGAGATTTTGAAACGCTGAGCGCCCTTTTAC
AGGACCGGGTCAAGGCTCTGGTGATTTTCGGCGAAGCGAGAGAACGGATTCAGGAGCGGATCGGCGGGATCGTTCCTACC
GTTTTAACGCCCTCCCTGAAGGAGGCCATTGCAGCCGCCCGTCGGCAGGCTTGTGCCGGGGATGTCGTCCTGTTGTCTCC
CGGGTGTGCCAGTTTTGATGAGTTTGCAGATTATAAGGCAAGAGGGAGATTCTTTAAGGAAGAAGTGAGGGCTTTTGCAT
GA

Upstream 100 bases:

>100_bases
GAATTGAAGGGATGGGCGGAGCCGAAGGTGATTGTCCGCTTCTGGATCATTTCCATCCTGCTGGCCCTGCTGGCCATCAG
TACACTAAAACTTCGATAGG

Downstream 100 bases:

>100_bases
TCGGCTTTCTGAAAAGATTTTCGCTGCCGGAACTGCCATTTCGCAATCTCCCGGAGGGCGATAAACGACCGGATCTTGTT
CTCCTGCTCGTGGTCCTGAT

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MFFMDISGQRMLVIGWGKTGVASARFLISRGARVVVADEKNLSLENDVLSKLGEDHGQSVELADYGISALAQVDAVVPSP
GVPPFHPVLKEAVKRCIPVISELELACRYLQTPMIAITGTNGKTTTTSLIGEILSGSGRKVFVGGNIGTPLVEYVTGPQT
ADCAVVEISSFQLQWVDQFHAFVSILLNTTCDHVNYHGSFEAYRAVKERIFNNQGKQDVAILNADEPDSAVLAESLSSPV
FFFSTTKMVERGLYQEKDRLICLDGEGKQEFYPLSMIRLPGAHNVENVMAAILASRACGCSPENVINAISGFSGIAHRIE
FTREIGGVKFYDDSKGTNVGAVKRAIETFSDPIILLMGGRDKDGDFETLSALLQDRVKALVIFGEARERIQERIGGIVPT
VLTPSLKEAIAAARRQACAGDVVLLSPGCASFDEFADYKARGRFFKEEVRAFA

Sequences:

>Translated_453_residues
MFFMDISGQRMLVIGWGKTGVASARFLISRGARVVVADEKNLSLENDVLSKLGEDHGQSVELADYGISALAQVDAVVPSP
GVPPFHPVLKEAVKRCIPVISELELACRYLQTPMIAITGTNGKTTTTSLIGEILSGSGRKVFVGGNIGTPLVEYVTGPQT
ADCAVVEISSFQLQWVDQFHAFVSILLNTTCDHVNYHGSFEAYRAVKERIFNNQGKQDVAILNADEPDSAVLAESLSSPV
FFFSTTKMVERGLYQEKDRLICLDGEGKQEFYPLSMIRLPGAHNVENVMAAILASRACGCSPENVINAISGFSGIAHRIE
FTREIGGVKFYDDSKGTNVGAVKRAIETFSDPIILLMGGRDKDGDFETLSALLQDRVKALVIFGEARERIQERIGGIVPT
VLTPSLKEAIAAARRQACAGDVVLLSPGCASFDEFADYKARGRFFKEEVRAFA
>Mature_453_residues
MFFMDISGQRMLVIGWGKTGVASARFLISRGARVVVADEKNLSLENDVLSKLGEDHGQSVELADYGISALAQVDAVVPSP
GVPPFHPVLKEAVKRCIPVISELELACRYLQTPMIAITGTNGKTTTTSLIGEILSGSGRKVFVGGNIGTPLVEYVTGPQT
ADCAVVEISSFQLQWVDQFHAFVSILLNTTCDHVNYHGSFEAYRAVKERIFNNQGKQDVAILNADEPDSAVLAESLSSPV
FFFSTTKMVERGLYQEKDRLICLDGEGKQEFYPLSMIRLPGAHNVENVMAAILASRACGCSPENVINAISGFSGIAHRIE
FTREIGGVKFYDDSKGTNVGAVKRAIETFSDPIILLMGGRDKDGDFETLSALLQDRVKALVIFGEARERIQERIGGIVPT
VLTPSLKEAIAAARRQACAGDVVLLSPGCASFDEFADYKARGRFFKEEVRAFA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=447, Percent_Identity=32.8859060402685, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1790680, Length=303, Percent_Identity=27.3927392739274, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_SYNAS (Q2LR50)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_460726.1
- ProteinModelPortal:   Q2LR50
- STRING:   Q2LR50
- GeneID:   3885151
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_01744
- NMPDR:   fig|56780.10.peg.671
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q2LR50
- BioCyc:   SACI56780:SYN_01744-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49141; Mature: 49141

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFMDISGQRMLVIGWGKTGVASARFLISRGARVVVADEKNLSLENDVLSKLGEDHGQSV
CEEEEECCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCCCEE
ELADYGISALAQVDAVVPSPGVPPFHPVLKEAVKRCIPVISELELACRYLQTPMIAITGT
EEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NGKTTTTSLIGEILSGSGRKVFVGGNIGTPLVEYVTGPQTADCAVVEISSFQLQWVDQFH
CCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCEEEEEECCHHHHHHHHHH
AFVSILLNTTCDHVNYHGSFEAYRAVKERIFNNQGKQDVAILNADEPDSAVLAESLSSPV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCE
FFFSTTKMVERGLYQEKDRLICLDGEGKQEFYPLSMIRLPGAHNVENVMAAILASRACGC
EEEEHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCC
SPENVINAISGFSGIAHRIEFTREIGGVKFYDDSKGTNVGAVKRAIETFSDPIILLMGGR
CHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCC
DKDGDFETLSALLQDRVKALVIFGEARERIQERIGGIVPTVLTPSLKEAIAAARRQACAG
CCCCCHHHHHHHHHHHHHEEEEEHHHHHHHHHHHCCEEHHHCCHHHHHHHHHHHHHHCCC
DVVLLSPGCASFDEFADYKARGRFFKEEVRAFA
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFFMDISGQRMLVIGWGKTGVASARFLISRGARVVVADEKNLSLENDVLSKLGEDHGQSV
CEEEEECCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCCCEE
ELADYGISALAQVDAVVPSPGVPPFHPVLKEAVKRCIPVISELELACRYLQTPMIAITGT
EEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NGKTTTTSLIGEILSGSGRKVFVGGNIGTPLVEYVTGPQTADCAVVEISSFQLQWVDQFH
CCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCEEEEEECCHHHHHHHHHH
AFVSILLNTTCDHVNYHGSFEAYRAVKERIFNNQGKQDVAILNADEPDSAVLAESLSSPV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCE
FFFSTTKMVERGLYQEKDRLICLDGEGKQEFYPLSMIRLPGAHNVENVMAAILASRACGC
EEEEHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCC
SPENVINAISGFSGIAHRIEFTREIGGVKFYDDSKGTNVGAVKRAIETFSDPIILLMGGR
CHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCC
DKDGDFETLSALLQDRVKALVIFGEARERIQERIGGIVPTVLTPSLKEAIAAARRQACAG
CCCCCHHHHHHHHHHHHHEEEEEHHHHHHHHHHHCCEEHHHCCHHHHHHHHHHHHHHCCC
DVVLLSPGCASFDEFADYKARGRFFKEEVRAFA
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA