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The map label for this gene is pheS

Identifier: 85858501

GI number: 85858501

Start: 656245

End: 657261

Strand: Direct

Name: pheS

Synonym: SYN_01721

Alternate gene names: 85858501

Gene position: 656245-657261 (Clockwise)

Preceding gene: 85858500

Following gene: 85858502

Centisome position: 20.64

GC content: 49.26

Gene sequence:

>1017_bases
ATGATTGAAGATCTCAAGGAGCTGCAAAATAAGGCGGAAGCGGAGATAAAAGCAGCGAAAACGGAAACGGATCTGGCTGC
TGTTCGGGTTCGATATCTGGGGAGAAAGGGGTTGCTGACTCAGTTTTTGCGCAATCTGGGTCAGTTGAGCATCGAGGAAA
AGCAGATTTTTGGAAAGCGCGCCAACGAAATCAAAACAATCCTGTCTTCGAGATTTGACGAAGCGCAGACAGTTATAGCC
GCCCTCAAAAAAGAAAGCGTCCTTCGGGCGGAAGCTCTTGATGTGACCCTGCCCGGAAGGAGCAGCGGCTACGGCCGGGC
TCATCCAGTCACTCAGGTCCTTGAGGAGATCTGTGCGATATTCTCCGAAATCGGATTCTCCATTGCTGAAGGTCCTGAAA
TAGAGAGTGATTATTATAATTTTGAGGCCTTAAACATCCCCAAGGACCATCCCGCCAGAGATATGCAGGATACATTTTAT
GTGGAAGAAGCGATCGTTCTGCGCACCCATACCTCTCCCGTACAAATCAGAACCATGGAAAAGCAGTCGCCACCGGTGAA
AATCATTTCGCCGGGGCGTGTATATCGTCCGGATTCTGATGTTTCTCATACGCCGATGTTTCATCAGGTTGAAGGCCTGC
TGGTTGACAGGGGAATTACCTTCGGCGACCTGAAAGGGCTTCTGAACGCTTTCCTTAAACAGATTTTTGGGGAAGAAACG
GTTCTTCGTTTCAGGCCCAGTTATTTTCCCTTTACGGAGCCCAGTGCGGAAGTGGATATCCGTTGTGTCATCTGTCGGGG
AAAAGGTTGTCGAGTTTGCGGGCAGAGCGGCTGGCTGGAAATCCTCGGATCGGGCATGGTGGACCCGGCAGTTTTTGAAA
ATGTCGGTTACGATCCTGAGGAATATTCGGGCTTTGCCTTTGGCTTGGGCGTAGAACGCATCGCCATGCTCAAGTATGGA
ATTACTGACATTCGACTTTTCTTTGAGAATGAAAGAAGGTTTTTACAGCAGTTCTAA

Upstream 100 bases:

>100_bases
CGCACGTGGAAATTGACCGGAAAGTGTTGGCGGATATCGCCGTTAACGACCCCAGGGGATTCTCAGAAATTGTTCTTCTT
GCGAAGGGTGAGACTGTTTC

Downstream 100 bases:

>100_bases
TGTAAAAGCCGCTGTCGTTGAATTTCCTGAATCGCGAAGACAAAAAAACAGGAGCGTCGGGCCAGGATTCTGTCCATACC
CTTTCTCCTATGAAAATGCA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MIEDLKELQNKAEAEIKAAKTETDLAAVRVRYLGRKGLLTQFLRNLGQLSIEEKQIFGKRANEIKTILSSRFDEAQTVIA
ALKKESVLRAEALDVTLPGRSSGYGRAHPVTQVLEEICAIFSEIGFSIAEGPEIESDYYNFEALNIPKDHPARDMQDTFY
VEEAIVLRTHTSPVQIRTMEKQSPPVKIISPGRVYRPDSDVSHTPMFHQVEGLLVDRGITFGDLKGLLNAFLKQIFGEET
VLRFRPSYFPFTEPSAEVDIRCVICRGKGCRVCGQSGWLEILGSGMVDPAVFENVGYDPEEYSGFAFGLGVERIAMLKYG
ITDIRLFFENERRFLQQF

Sequences:

>Translated_338_residues
MIEDLKELQNKAEAEIKAAKTETDLAAVRVRYLGRKGLLTQFLRNLGQLSIEEKQIFGKRANEIKTILSSRFDEAQTVIA
ALKKESVLRAEALDVTLPGRSSGYGRAHPVTQVLEEICAIFSEIGFSIAEGPEIESDYYNFEALNIPKDHPARDMQDTFY
VEEAIVLRTHTSPVQIRTMEKQSPPVKIISPGRVYRPDSDVSHTPMFHQVEGLLVDRGITFGDLKGLLNAFLKQIFGEET
VLRFRPSYFPFTEPSAEVDIRCVICRGKGCRVCGQSGWLEILGSGMVDPAVFENVGYDPEEYSGFAFGLGVERIAMLKYG
ITDIRLFFENERRFLQQF
>Mature_338_residues
MIEDLKELQNKAEAEIKAAKTETDLAAVRVRYLGRKGLLTQFLRNLGQLSIEEKQIFGKRANEIKTILSSRFDEAQTVIA
ALKKESVLRAEALDVTLPGRSSGYGRAHPVTQVLEEICAIFSEIGFSIAEGPEIESDYYNFEALNIPKDHPARDMQDTFY
VEEAIVLRTHTSPVQIRTMEKQSPPVKIISPGRVYRPDSDVSHTPMFHQVEGLLVDRGITFGDLKGLLNAFLKQIFGEET
VLRFRPSYFPFTEPSAEVDIRCVICRGKGCRVCGQSGWLEILGSGMVDPAVFENVGYDPEEYSGFAFGLGVERIAMLKYG
ITDIRLFFENERRFLQQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=34.5724907063197, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI5729820, Length=289, Percent_Identity=30.7958477508651, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=51.7751479289941, Blast_Score=346, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17508957, Length=269, Percent_Identity=33.457249070632, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32563657, Length=269, Percent_Identity=33.457249070632, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32566635, Length=256, Percent_Identity=33.203125, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321087, Length=370, Percent_Identity=27.2972972972973, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325304, Length=327, Percent_Identity=27.82874617737, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18858079, Length=285, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137424, Length=248, Percent_Identity=28.6290322580645, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_SYNAS (Q2LR28)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_460703.1
- ProteinModelPortal:   Q2LR28
- SMR:   Q2LR28
- STRING:   Q2LR28
- GeneID:   3885457
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_01721
- NMPDR:   fig|56780.10.peg.650
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q2LR28
- BioCyc:   SACI56780:SYN_01721-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38100; Mature: 38100

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEDLKELQNKAEAEIKAAKTETDLAAVRVRYLGRKGLLTQFLRNLGQLSIEEKQIFGKR
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHH
ANEIKTILSSRFDEAQTVIAALKKESVLRAEALDVTLPGRSSGYGRAHPVTQVLEEICAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHH
FSEIGFSIAEGPEIESDYYNFEALNIPKDHPARDMQDTFYVEEAIVLRTHTSPVQIRTME
HHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHEEEEEECCCCEEEEEEC
KQSPPVKIISPGRVYRPDSDVSHTPMFHQVEGLLVDRGITFGDLKGLLNAFLKQIFGEET
CCCCCEEEECCCEEECCCCCCCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCE
VLRFRPSYFPFTEPSAEVDIRCVICRGKGCRVCGQSGWLEILGSGMVDPAVFENVGYDPE
EEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHCCCCHH
EYSGFAFGLGVERIAMLKYGITDIRLFFENERRFLQQF
HHCCEEEHHHHHHHHHHHHCCHHEEEEEHHHHHHHHCC
>Mature Secondary Structure
MIEDLKELQNKAEAEIKAAKTETDLAAVRVRYLGRKGLLTQFLRNLGQLSIEEKQIFGKR
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHH
ANEIKTILSSRFDEAQTVIAALKKESVLRAEALDVTLPGRSSGYGRAHPVTQVLEEICAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHH
FSEIGFSIAEGPEIESDYYNFEALNIPKDHPARDMQDTFYVEEAIVLRTHTSPVQIRTME
HHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHEEEEEECCCCEEEEEEC
KQSPPVKIISPGRVYRPDSDVSHTPMFHQVEGLLVDRGITFGDLKGLLNAFLKQIFGEET
CCCCCEEEECCCEEECCCCCCCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCE
VLRFRPSYFPFTEPSAEVDIRCVICRGKGCRVCGQSGWLEILGSGMVDPAVFENVGYDPE
EEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHCCCCHH
EYSGFAFGLGVERIAMLKYGITDIRLFFENERRFLQQF
HHCCEEEHHHHHHHHHHHHCCHHEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA