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The map label for this gene is cca [H]

Identifier: 85858488

GI number: 85858488

Start: 643544

End: 646171

Strand: Direct

Name: cca [H]

Synonym: SYN_00533

Alternate gene names: 85858488

Gene position: 643544-646171 (Clockwise)

Preceding gene: 85858487

Following gene: 85858491

Centisome position: 20.24

GC content: 54.11

Gene sequence:

>2628_bases
ATGGATATTATTACATCGCATATACAGACTGATTTCGATTCCCTGGGTTCCATGCTGGCGGCGAAGCTGCTGTACCCGGA
AGCCCTGATCGTTTTTCCCGGGGCGCAGGAGAAGAGCGTCCGTGATTACCTGATGAACGCTCCTGCGGCAATCCGGGAAG
ACATGGTGAAACTCCGCCAGGTTGATCTGGACGATGTCAAGAGACTGATCATTGTGGACACGCGTCAGGCATCCCGCATC
GGGGCTTTTGCGGATCTTCTCGGTCGGGAAGGCGTGACGGTTGTCCTTTATGATCATCATGGTTCCTCTCCTGACGATAT
CCAGGGGGATTCAGAGTACTGCGAGGAACTGGGTTCCACGGTAGCCCTCATGGTGAAGATACTGAACCAAGAGGGAATAA
TGCCATCTCCTGAGATTGCCACAGTCATGGCCATGGGACTCTATGAAGATACCGGTTCCCTGCTTTTCCCCTCTGCGACG
CCGGACGACTGCATGGCTCTGGCCGCTCTGATGACCTGGGGTGCGGACATGGATTCGATATCGAGGACCATTTCCCGGGA
ACTCAGCGCGGAGCAGGTGAATGTGCTGGATCAGCTGATCAAAAACCTTCGGATGCTCGAGGTCAACGGCTTGCATGTCG
CGGTTTCTAAGTCCGCCTCAACGGCTTACGTGGAGGATTTTGCCATATTGGTTCACAAATTAAGGGGCATGTATGTCGCA
GATGCCTTTTTTGCGCTTGGACAAATGGGGGAACGGATCTATCTGGTTGCCCGGAGTACGCAACCGCAGCTCAATGCGGC
GCAGATTGCCGCTTTCTTCGGCGGCGGTGGTCATGCCTCGGCCGCGGCGGCTTCTCTTAAGGAACTCAATCTCAATCAGG
CGGAATCCCTGCTGATCAAATATATCCGGCAGGAAACGAGGCCCCTTATTACCGCCCGGGACATCATGACGTTTCCAGTC
AAGGGGATTTATCTGGAAGACAGCATTAACAAAGCCAGCGAACTTCTCAACAAATATCACATCAATGCCCTGATTGTGAA
ATCCGCCGATGAACGGGTCCGGGGCATGATCACCCGTCAGGTGGTCAGTCGCGCTCAGGGGCACGATCTGGGAGAGGCCA
GAATCAGGGATTACATGATCCAGGATGTGCGCACCGTTCGACCGGATGCTCCCGTGGGAGAAATCCGGAGTCTGGTGATT
GACGGAAATCAGCGGCTTATCCCCGTGGTTGAAGACCGCAGGGCCGTGGGGGTGATCACCCGTACGGACCTGATGAGGAT
GCTTCATGAAAGAATGACTAAGATCGAAAAGGGGGTGTCCGCAGAAGCTCCAGGAAAATCAATCCGGCACCTTCTTATGG
AGAGGATGCCCCCGGATTTATACGACACGCTCAAGAAATCCGGAGAACTGGCAGAGTCCATGGGATTCAATCTCTTTCTC
GTAGGAGGGATTGTCCGGGATATGGTTATGCGGATTGAAAATCTGGATGTCGACCTTGTTGTCGAAGGTGACGGGATTAT
CTTTGCAAAGGCCCTCGCCGAAGCCTTCGGCGGGAAGGTTTCGCCTCACGAAAAGTACAAAACTGCGGTTGTCCGGCTTC
CGGGAGGGCGGAGGATCGATCTGGCCACGGCAAGACTGGAGTACTACCGGATGCCGGGGGCCTTCCCGGAGGTAGAGGAA
TCGACACTGAAACTTGATCTCTACCGGAGAGACTTTACGATCAACACAATGGCTGTGGCCCTGAATCCCCATCGTTTCGG
GGAATTAATCGATTTTTTCGGGGCGCAGCGGGACATCCGGGAAAAAAAGATCCGGGTGCTCCATTCCCTGAGCTTTGTGG
AGGATCCTTCACGCATCCTCCGGGCTGTCCGGTTTGAAAAACGCTTTAACTTTTGTCTGGGTAAACAGACAGTCTCGCTG
ATTTCCTCGGCCGTTCAGGCCGGTTTTCTCCGTAATATACCAGGAAGGCGGATCTTTCACGAAATGCGGCAGATACTGCA
GGAAGAAAATCCGGTCCGCTGCATGGACCGGCTTCATGAACTCAAGGTCCTGGAAGCCATCCATCCGGACCTGGTTTTCG
ATGAACGGATTCGAGAATCATTTGTTTCCGTTCGAGAAACCCTTTTCTGGTTTCGCCTGCTATACACCCATGAAAAAGTC
CGGCCCTGGTTTCTCTATTGTCTGGCTCTGACGGAACCGATGAATCCCGAGACGGTCATGTCAATTGCAGAAGCTCTTGG
GCTGAGGCGGGAAGATGCGGAAATGCTCCTTGCCTCACGGGAAAGAGTCCGGGAACTCATGAAGCGGCTGGCATCCCTGT
CTGAAATCCTGCCGTCCAAGGCGTTTGGAATCCTGGAAGACGCGGCCCTTGAGGAGATGCTTTACGCCATGTCCCGGTCA
CGGTCGGCAACCTTCAAAAAGATCTTTTCCACTTATGTGACCTCCTGGCGGAACTATCGGCCTCCGGTCAGCGGCAAGGA
TCTTCTTGTTATTGGATTTTCACAGAATCCGGAGCTGGGCCGCTGTTTAAGGGAAATCCGCGACCAGGGATTAAACGGAG
AGATCCGGGACTTCGCAGGCGCCATGGACTATGCCCGAACCTTCCTGAGAGGCCTGCGCCAATCGTGA

Upstream 100 bases:

>100_bases
AGTCACCGTGATTGATCTTGTCCCGGCCGGTGTTCTGCCGAGGGAATCCTTGGGCGGCAGCAGAGTACACTTCTCCAGCG
GGAAGGACTGATCACCGATC

Downstream 100 bases:

>100_bases
CATTTCTGTTTCCCGAATCGGTTTCATGCCTGCTCGCATAACAATAAGGATGACCGCCAATCCCTCCCCTCAATCTCTAT
CAAAAATGGGTAAGCGCGGG

Product: tRNA nucleotidyltransferase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 875; Mature: 875

Protein sequence:

>875_residues
MDIITSHIQTDFDSLGSMLAAKLLYPEALIVFPGAQEKSVRDYLMNAPAAIREDMVKLRQVDLDDVKRLIIVDTRQASRI
GAFADLLGREGVTVVLYDHHGSSPDDIQGDSEYCEELGSTVALMVKILNQEGIMPSPEIATVMAMGLYEDTGSLLFPSAT
PDDCMALAALMTWGADMDSISRTISRELSAEQVNVLDQLIKNLRMLEVNGLHVAVSKSASTAYVEDFAILVHKLRGMYVA
DAFFALGQMGERIYLVARSTQPQLNAAQIAAFFGGGGHASAAAASLKELNLNQAESLLIKYIRQETRPLITARDIMTFPV
KGIYLEDSINKASELLNKYHINALIVKSADERVRGMITRQVVSRAQGHDLGEARIRDYMIQDVRTVRPDAPVGEIRSLVI
DGNQRLIPVVEDRRAVGVITRTDLMRMLHERMTKIEKGVSAEAPGKSIRHLLMERMPPDLYDTLKKSGELAESMGFNLFL
VGGIVRDMVMRIENLDVDLVVEGDGIIFAKALAEAFGGKVSPHEKYKTAVVRLPGGRRIDLATARLEYYRMPGAFPEVEE
STLKLDLYRRDFTINTMAVALNPHRFGELIDFFGAQRDIREKKIRVLHSLSFVEDPSRILRAVRFEKRFNFCLGKQTVSL
ISSAVQAGFLRNIPGRRIFHEMRQILQEENPVRCMDRLHELKVLEAIHPDLVFDERIRESFVSVRETLFWFRLLYTHEKV
RPWFLYCLALTEPMNPETVMSIAEALGLRREDAEMLLASRERVRELMKRLASLSEILPSKAFGILEDAALEEMLYAMSRS
RSATFKKIFSTYVTSWRNYRPPVSGKDLLVIGFSQNPELGRCLREIRDQGLNGEIRDFAGAMDYARTFLRGLRQS

Sequences:

>Translated_875_residues
MDIITSHIQTDFDSLGSMLAAKLLYPEALIVFPGAQEKSVRDYLMNAPAAIREDMVKLRQVDLDDVKRLIIVDTRQASRI
GAFADLLGREGVTVVLYDHHGSSPDDIQGDSEYCEELGSTVALMVKILNQEGIMPSPEIATVMAMGLYEDTGSLLFPSAT
PDDCMALAALMTWGADMDSISRTISRELSAEQVNVLDQLIKNLRMLEVNGLHVAVSKSASTAYVEDFAILVHKLRGMYVA
DAFFALGQMGERIYLVARSTQPQLNAAQIAAFFGGGGHASAAAASLKELNLNQAESLLIKYIRQETRPLITARDIMTFPV
KGIYLEDSINKASELLNKYHINALIVKSADERVRGMITRQVVSRAQGHDLGEARIRDYMIQDVRTVRPDAPVGEIRSLVI
DGNQRLIPVVEDRRAVGVITRTDLMRMLHERMTKIEKGVSAEAPGKSIRHLLMERMPPDLYDTLKKSGELAESMGFNLFL
VGGIVRDMVMRIENLDVDLVVEGDGIIFAKALAEAFGGKVSPHEKYKTAVVRLPGGRRIDLATARLEYYRMPGAFPEVEE
STLKLDLYRRDFTINTMAVALNPHRFGELIDFFGAQRDIREKKIRVLHSLSFVEDPSRILRAVRFEKRFNFCLGKQTVSL
ISSAVQAGFLRNIPGRRIFHEMRQILQEENPVRCMDRLHELKVLEAIHPDLVFDERIRESFVSVRETLFWFRLLYTHEKV
RPWFLYCLALTEPMNPETVMSIAEALGLRREDAEMLLASRERVRELMKRLASLSEILPSKAFGILEDAALEEMLYAMSRS
RSATFKKIFSTYVTSWRNYRPPVSGKDLLVIGFSQNPELGRCLREIRDQGLNGEIRDFAGAMDYARTFLRGLRQS
>Mature_875_residues
MDIITSHIQTDFDSLGSMLAAKLLYPEALIVFPGAQEKSVRDYLMNAPAAIREDMVKLRQVDLDDVKRLIIVDTRQASRI
GAFADLLGREGVTVVLYDHHGSSPDDIQGDSEYCEELGSTVALMVKILNQEGIMPSPEIATVMAMGLYEDTGSLLFPSAT
PDDCMALAALMTWGADMDSISRTISRELSAEQVNVLDQLIKNLRMLEVNGLHVAVSKSASTAYVEDFAILVHKLRGMYVA
DAFFALGQMGERIYLVARSTQPQLNAAQIAAFFGGGGHASAAAASLKELNLNQAESLLIKYIRQETRPLITARDIMTFPV
KGIYLEDSINKASELLNKYHINALIVKSADERVRGMITRQVVSRAQGHDLGEARIRDYMIQDVRTVRPDAPVGEIRSLVI
DGNQRLIPVVEDRRAVGVITRTDLMRMLHERMTKIEKGVSAEAPGKSIRHLLMERMPPDLYDTLKKSGELAESMGFNLFL
VGGIVRDMVMRIENLDVDLVVEGDGIIFAKALAEAFGGKVSPHEKYKTAVVRLPGGRRIDLATARLEYYRMPGAFPEVEE
STLKLDLYRRDFTINTMAVALNPHRFGELIDFFGAQRDIREKKIRVLHSLSFVEDPSRILRAVRFEKRFNFCLGKQTVSL
ISSAVQAGFLRNIPGRRIFHEMRQILQEENPVRCMDRLHELKVLEAIHPDLVFDERIRESFVSVRETLFWFRLLYTHEKV
RPWFLYCLALTEPMNPETVMSIAEALGLRREDAEMLLASRERVRELMKRLASLSEILPSKAFGILEDAALEEMLYAMSRS
RSATFKKIFSTYVTSWRNYRPPVSGKDLLVIGFSQNPELGRCLREIRDQGLNGEIRDFAGAMDYARTFLRGLRQS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=225, Percent_Identity=29.7777777777778, Blast_Score=73, Evalue=9e-14,
Organism=Escherichia coli, GI87081691, Length=352, Percent_Identity=25.5681818181818, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321016, Length=242, Percent_Identity=29.3388429752066, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 98605; Mature: 98605

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIITSHIQTDFDSLGSMLAAKLLYPEALIVFPGAQEKSVRDYLMNAPAAIREDMVKLRQ
CCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHH
VDLDDVKRLIIVDTRQASRIGAFADLLGREGVTVVLYDHHGSSPDDIQGDSEYCEELGST
CCHHHCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
VALMVKILNQEGIMPSPEIATVMAMGLYEDTGSLLFPSATPDDCMALAALMTWGADMDSI
HHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCCEEECCCCCHHHHHHHHHHHCCCCHHHH
SRTISRELSAEQVNVLDQLIKNLRMLEVNGLHVAVSKSASTAYVEDFAILVHKLRGMYVA
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
DAFFALGQMGERIYLVARSTQPQLNAAQIAAFFGGGGHASAAAASLKELNLNQAESLLIK
HHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHH
YIRQETRPLITARDIMTFPVKGIYLEDSINKASELLNKYHINALIVKSADERVRGMITRQ
HHHHHHCCCEEHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHH
VVSRAQGHDLGEARIRDYMIQDVRTVRPDAPVGEIRSLVIDGNQRLIPVVEDRRAVGVIT
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHEECCCCEEEEEECCCCCEEEHH
RTDLMRMLHERMTKIEKGVSAEAPGKSIRHLLMERMPPDLYDTLKKSGELAESMGFNLFL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCEEE
VGGIVRDMVMRIENLDVDLVVEGDGIIFAKALAEAFGGKVSPHEKYKTAVVRLPGGRRID
HHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHCEEEEECCCCCEEE
LATARLEYYRMPGAFPEVEESTLKLDLYRRDFTINTMAVALNPHRFGELIDFFGAQRDIR
HHHHHHHHHCCCCCCCCCCCCCEEEEEEECCEEEEEEEEEECCHHHHHHHHHHCCHHHHH
EKKIRVLHSLSFVEDPSRILRAVRFEKRFNFCLGKQTVSLISSAVQAGFLRNIPGRRIFH
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EMRQILQEENPVRCMDRLHELKVLEAIHPDLVFDERIRESFVSVRETLFWFRLLYTHEKV
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPWFLYCLALTEPMNPETVMSIAEALGLRREDAEMLLASRERVRELMKRLASLSEILPSK
CHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGILEDAALEEMLYAMSRSRSATFKKIFSTYVTSWRNYRPPVSGKDLLVIGFSQNPELG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHH
RCLREIRDQGLNGEIRDFAGAMDYARTFLRGLRQS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIITSHIQTDFDSLGSMLAAKLLYPEALIVFPGAQEKSVRDYLMNAPAAIREDMVKLRQ
CCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHH
VDLDDVKRLIIVDTRQASRIGAFADLLGREGVTVVLYDHHGSSPDDIQGDSEYCEELGST
CCHHHCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
VALMVKILNQEGIMPSPEIATVMAMGLYEDTGSLLFPSATPDDCMALAALMTWGADMDSI
HHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCCEEECCCCCHHHHHHHHHHHCCCCHHHH
SRTISRELSAEQVNVLDQLIKNLRMLEVNGLHVAVSKSASTAYVEDFAILVHKLRGMYVA
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
DAFFALGQMGERIYLVARSTQPQLNAAQIAAFFGGGGHASAAAASLKELNLNQAESLLIK
HHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHH
YIRQETRPLITARDIMTFPVKGIYLEDSINKASELLNKYHINALIVKSADERVRGMITRQ
HHHHHHCCCEEHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHH
VVSRAQGHDLGEARIRDYMIQDVRTVRPDAPVGEIRSLVIDGNQRLIPVVEDRRAVGVIT
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHEECCCCEEEEEECCCCCEEEHH
RTDLMRMLHERMTKIEKGVSAEAPGKSIRHLLMERMPPDLYDTLKKSGELAESMGFNLFL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCEEE
VGGIVRDMVMRIENLDVDLVVEGDGIIFAKALAEAFGGKVSPHEKYKTAVVRLPGGRRID
HHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHCEEEEECCCCCEEE
LATARLEYYRMPGAFPEVEESTLKLDLYRRDFTINTMAVALNPHRFGELIDFFGAQRDIR
HHHHHHHHHCCCCCCCCCCCCCEEEEEEECCEEEEEEEEEECCHHHHHHHHHHCCHHHHH
EKKIRVLHSLSFVEDPSRILRAVRFEKRFNFCLGKQTVSLISSAVQAGFLRNIPGRRIFH
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EMRQILQEENPVRCMDRLHELKVLEAIHPDLVFDERIRESFVSVRETLFWFRLLYTHEKV
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPWFLYCLALTEPMNPETVMSIAEALGLRREDAEMLLASRERVRELMKRLASLSEILPSK
CHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGILEDAALEEMLYAMSRSRSATFKKIFSTYVTSWRNYRPPVSGKDLLVIGFSQNPELG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHH
RCLREIRDQGLNGEIRDFAGAMDYARTFLRGLRQS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA