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The map label for this gene is dacF [H]

Identifier: 85858430

GI number: 85858430

Start: 582834

End: 583766

Strand: Direct

Name: dacF [H]

Synonym: SYN_02260

Alternate gene names: 85858430

Gene position: 582834-583766 (Clockwise)

Preceding gene: 85858429

Following gene: 85858432

Centisome position: 18.33

GC content: 53.27

Gene sequence:

>933_bases
ATGAAAAAATTAGCGATTTTATTTTCCATTTTACTTACGGTCTTTTTTATCCAGCCCTCTGAACTCATGGCTCGGAAAGC
ACCAAAGAAGAAAACGCCGGAGGTTAAAGCCGCAGTCGGGAAATCCGTCTCCAAGAAGAGAGTCGCCCGGAAAAGTCCCA
GAAACACGGCTCCCGAGATCAAGTCCTCCATCCTGATGGACATGACCGACAGGCGGGTCCTTTACGAGCAGAATGCAGAC
CAGCCTATTCAACCGGCGTCTTTGACTAAAGTGCTTTCTCTGTACCTGACTTACGAGGCCATGGCTCAGGGCAGGGTTCG
CTGGCTGGATTCCGTGGTTGTCAGTCAAGCGGCCCGGCAGGCGGATGGATCACGGATGTATATAGAAGCAGGGGAGAGGG
TTCCTCTGGAAACCCTGATTAAGGGAATGACGGTCATCTCGGCGAATGATGCCTCCGTGGCCGTAGCTGAGCATGTCGCG
GGCAATGTGGACCGCTTTGTCGAAAGGATGAACCGCAAGGCCGGTGAACTGGGAATGACGCACAGCGTCTTCCGGACTCC
CAACGGCCTGCCTGCGGAAGGGCAATATACCACGGCAAGGGATATGATGATTCTGGCCGATGCCTATCTTCGACGCTTTC
CTGGTTCGCTGGCCCTTCATTCCATGCAGTATTACACCTATAAAAAAATCACCCAGCACAACTGCAATGCCCTTCTGAAA
AAATATCCCGATGTGGATGGGCTGAAAAGCGGTTTTGTGAGGGCTTCCGGATACAATCTTATTGCCACGGCAAGGCGGGG
CAATACCCGCCTTATCGCTGTTGTCATGGGAGCGAGGACTCCTGAAATCCGGGTGCGGGAAACCCGCAGGCTTCTGGATG
CCGGATTTCAGATGCTTCAGGCAAGCCGCACTGGCGTTTCCTTGAAATCCTGA

Upstream 100 bases:

>100_bases
ATATCGCCTCCGGACGAAACTGGGATGAGGCTCACAGTTAAAAAATCCCCTCGGCAGGCGGGAACCCGGTAAAATGATGT
AAAGAAGGAGTATCAAGAGA

Downstream 100 bases:

>100_bases
GGGCCTGCGCGGCTGTATGCTGCGAAAAAAGCCTTTAGTGCGCTGTGCGGTAATAGAACGTTATTTGTGCTATTACAGCA
CAGTATATATTATGTTGATA

Product: D-alanyl-D-alanine serine-type carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKKLAILFSILLTVFFIQPSELMARKAPKKKTPEVKAAVGKSVSKKRVARKSPRNTAPEIKSSILMDMTDRRVLYEQNAD
QPIQPASLTKVLSLYLTYEAMAQGRVRWLDSVVVSQAARQADGSRMYIEAGERVPLETLIKGMTVISANDASVAVAEHVA
GNVDRFVERMNRKAGELGMTHSVFRTPNGLPAEGQYTTARDMMILADAYLRRFPGSLALHSMQYYTYKKITQHNCNALLK
KYPDVDGLKSGFVRASGYNLIATARRGNTRLIAVVMGARTPEIRVRETRRLLDAGFQMLQASRTGVSLKS

Sequences:

>Translated_310_residues
MKKLAILFSILLTVFFIQPSELMARKAPKKKTPEVKAAVGKSVSKKRVARKSPRNTAPEIKSSILMDMTDRRVLYEQNAD
QPIQPASLTKVLSLYLTYEAMAQGRVRWLDSVVVSQAARQADGSRMYIEAGERVPLETLIKGMTVISANDASVAVAEHVA
GNVDRFVERMNRKAGELGMTHSVFRTPNGLPAEGQYTTARDMMILADAYLRRFPGSLALHSMQYYTYKKITQHNCNALLK
KYPDVDGLKSGFVRASGYNLIATARRGNTRLIAVVMGARTPEIRVRETRRLLDAGFQMLQASRTGVSLKS
>Mature_310_residues
MKKLAILFSILLTVFFIQPSELMARKAPKKKTPEVKAAVGKSVSKKRVARKSPRNTAPEIKSSILMDMTDRRVLYEQNAD
QPIQPASLTKVLSLYLTYEAMAQGRVRWLDSVVVSQAARQADGSRMYIEAGERVPLETLIKGMTVISANDASVAVAEHVA
GNVDRFVERMNRKAGELGMTHSVFRTPNGLPAEGQYTTARDMMILADAYLRRFPGSLALHSMQYYTYKKITQHNCNALLK
KYPDVDGLKSGFVRASGYNLIATARRGNTRLIAVVMGARTPEIRVRETRRLLDAGFQMLQASRTGVSLKS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=253, Percent_Identity=39.1304347826087, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI1786851, Length=252, Percent_Identity=38.8888888888889, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87082022, Length=249, Percent_Identity=40.1606425702811, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 34485; Mature: 34485

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLAILFSILLTVFFIQPSELMARKAPKKKTPEVKAAVGKSVSKKRVARKSPRNTAPEI
CHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCHHH
KSSILMDMTDRRVLYEQNADQPIQPASLTKVLSLYLTYEAMAQGRVRWLDSVVVSQAARQ
HHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ADGSRMYIEAGERVPLETLIKGMTVISANDASVAVAEHVAGNVDRFVERMNRKAGELGMT
CCCCEEEEECCCCCCHHHHHCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
HSVFRTPNGLPAEGQYTTARDMMILADAYLRRFPGSLALHSMQYYTYKKITQHNCNALLK
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KYPDVDGLKSGFVRASGYNLIATARRGNTRLIAVVMGARTPEIRVRETRRLLDAGFQMLQ
HCCCCCCHHHCCEEECCCEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ASRTGVSLKS
HHHCCCCCCC
>Mature Secondary Structure
MKKLAILFSILLTVFFIQPSELMARKAPKKKTPEVKAAVGKSVSKKRVARKSPRNTAPEI
CHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCHHH
KSSILMDMTDRRVLYEQNADQPIQPASLTKVLSLYLTYEAMAQGRVRWLDSVVVSQAARQ
HHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ADGSRMYIEAGERVPLETLIKGMTVISANDASVAVAEHVAGNVDRFVERMNRKAGELGMT
CCCCEEEEECCCCCCHHHHHCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
HSVFRTPNGLPAEGQYTTARDMMILADAYLRRFPGSLALHSMQYYTYKKITQHNCNALLK
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KYPDVDGLKSGFVRASGYNLIATARRGNTRLIAVVMGARTPEIRVRETRRLLDAGFQMLQ
HCCCCCCHHHCCEEECCCEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ASRTGVSLKS
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]