| Definition | Syntrophus aciditrophicus SB chromosome, complete genome. |
|---|---|
| Accession | NC_007759 |
| Length | 3,179,300 |
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The map label for this gene is polA [H]
Identifier: 85858429
GI number: 85858429
Start: 580069
End: 582774
Strand: Direct
Name: polA [H]
Synonym: SYN_02261
Alternate gene names: 85858429
Gene position: 580069-582774 (Clockwise)
Preceding gene: 85858428
Following gene: 85858430
Centisome position: 18.25
GC content: 55.51
Gene sequence:
>2706_bases ATGTCAACAGGCGACAATACGGAAAACAGCGAAAAGAGTACGATTTATCTGGTCGACGGCAGCAATTATCTGTACCGGGC CTTTTATGCGATCCGGGAACTTTCCAATTCCAGGGGGCTTCCCACCAATGCCGTCTACGGATTCACCAATATGCTGATCA AGCTTCTCCGGGAGCGGAAACCGGAATACATTGCCATCACCTTTGATCTAAAGGGGCCGACCTTCCGGCATGAGACTTAC GCGCAATACAAAGCCACCCGGAAAGCGACCCCCGAGGCCCTGATTCCCCAGATCCCCTATATAAAGGACATCATCCGCGG TTTTTCCATCCCGATTCTTGAACAGCAGGGGACGGAAGCCGATGATATCATCGGCACCCTGGCCTGCCGTTTTGCCGGGG AAGGCCTAAAGGTCATCATCGTCTCCGGCGACAAGGACATGCTGCAGCTTCTTTCCCCCGATGTCATCATGATCGATACG ATGAACGACCGGACCTATGATGTCGCGGCCGTGAGGGAGCGTTTCGGCGTGGATCCGGAGAAGGTGCCCGAGATCCTGGG ACTGACGGGGGATATGTCGGATAACATTCCCGGCATTCCAGGCATTGGTCCGAAGGGTGCCTTGCGGCTCGTTGAAGAGT ACGGCTCGATCGAAGAGATTCTGCGGAATGTGGAACGCCTGAGGAATCCGAAACTCCGGGACAGTGTCCGGGTTTACGGT GAACAGGCCCTGCTGAGCCGCGATCTGGCCCGGATCCGCACCGGTATCCCGCTGACCTTTGATCTGGAAGAGGCCCGCCT CCAGGAACCGGATCGAGAGGTTCTGACTCGACTGTTCCGGGAACTGGAATTTTCCTCCCTGCTTCAGGAATTCAGCGTCG TTGAAGATTGCGGCGACAATTCCCATACACTGCTGCGAACACCGGAGGCTGTGGCGGCTTTCATGGAACCGCTGAAAGAG GGGGGATTTCTGGTCCTGGAAATGGTGCTTTCCTCGAGTGAACCAATGCGGGCGGAACTGATTGGAGTGGCCCTTGGACT GGATTCCGGGAACGCCGGTTATATACCTCTGGAACCTGGCGCAGCGGAAAAGAGCGCTTCAAGTCTTTCGGAAGAGCAGG TTTTTCCCGTCCTTTTTCCATTTCTAGCGGATAAAAAAATCGCCCTTGTCGGTCATGATCTCAAAACGGTTCTGATCGTC CTATCCCAAAAAGGGGTGAAGGTGCAGAATCCCCTCCTGGATACGATGGTGGCGGCCTACATCCTCAATCCTGCGCGGCG CGGGTATGAACTCTCCGAGATTGTGCAGGATTATCTGCACCGGCAGGTGGAGCCGTTGCGGAAAAAACTGGTCGGCGGGA GCTCGCCAAAACAGTCTCCTGCGTCCTTTCCGCCTGAACGTCTGCGGGATTTTGCCTGCCGGCGGGTGGAAGCTATTGGG GAGCTGGCTTCGACTCTGATTGAAAAAATGGAAGAGACGGGCCTCCGGGAACTGTTCGATTCGGTGGAGATGCCGCTGGT GGCGGTGCTGGCCGCCATGGAAGAAAAAGGGGTTCTCCTGGATTTGCATCTGCTGAAGGAGATGTCTCAGGAACTGGGTC ACCTTCTTTCTCTTTCGGAGGCGAAAATTTATGCCCTGGCGGGGGAGCGCTTCAATATCGCGTCTCCCAAGCAGCTCCAG ACCATCCTTTTTGAAAAGCTTAATCTGCCCAAGGGGAAAAAGACGAAGGGAGGATATTCTACGGATGTGGAGGTGTTGAG CAATCTTGCCCTGAACCATGAACTGCCGGCCGAGATCCTTGCCTACCGGAGCATGGCCAAGTTGAAATCCACGTATATCG ATGCCCTGCCTTCCCTGATCAACATCCGGACAGGACGGATTCACACCTCTTATAACCAGACGGTGGCAGCAACAGGTCGC CTCTCCAGCAGCAATCCCAATCTCCAGAACATCCCGATCCGCACCCTGGAGGGGAAGCGCATCCGGCAGGCTTTTATTGC CCCGCCAGGATGGGCGATCGTCTCCGCCGATTACTCTCAGATTGAATTGCGCGTGCTGGCCCATCTCTCCGAAGATCCGG TCCTGATTAATGCCTTTGCAGCCGGTGAAGACATCCACAGCCGGACGGCCGCCGATGTTTTCGGCGTGTTCCCGGAAATG ATCAACGCGGAGATGCGCCGTCAGGCGAAGGTCATCAATTTCGGCGTGCTTTATGGGATGAGCGCCTTTGGCCTGTCCCG GGAACTGGGGATTCCCCAGAAGCAGGCCCAGGCTTATATCGATGGATATTTTAAAAAGTACGAAAGCGTCAAAACCTATC TCGACGGCATCCTCCAACAGGCCAGGGAACGGGGCTATGTAACCACACTCCTTAACCGCCGCCGTTATCTTCCGGAAATC AACAGCGTCAATCCCGCGGTGCGTCAGCTGGCCGAGCGCATGGCCATCAATGCGCCCATTCAGGGGACAGCGGCTGATCT CATCAAGATGGCCATGCTCAGGATCGCAGACCTCATCAGGGAAAAGGGGATGAAGTCTGCCATGACGATGCAGGTTCATG ATGAACTGGTCTTTGAAGTGCCGCAGGAAGAAAAGATAGCGCTCATGAACCTGGTCCGGCAGGAGATGGAAGGGGTTGTC GAACTCAAGGTCCCCCTCAAAGTGGATATCGCCTCCGGACGAAACTGGGATGAGGCTCACAGTTAA
Upstream 100 bases:
>100_bases CAGAAATCTTAACCCAAACGGGTTGCTCAGGGTAGATTAAGAAGAGAGTCCTGTGTTATGTTGAACGAAAATTTCAGGAG ACTGAACAATACCGAATCTC
Downstream 100 bases:
>100_bases AAAATCCCCTCGGCAGGCGGGAACCCGGTAAAATGATGTAAAGAAGGAGTATCAAGAGAATGAAAAAATTAGCGATTTTA TTTTCCATTTTACTTACGGT
Product: DNA polymerase I
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 901; Mature: 900
Protein sequence:
>901_residues MSTGDNTENSEKSTIYLVDGSNYLYRAFYAIRELSNSRGLPTNAVYGFTNMLIKLLRERKPEYIAITFDLKGPTFRHETY AQYKATRKATPEALIPQIPYIKDIIRGFSIPILEQQGTEADDIIGTLACRFAGEGLKVIIVSGDKDMLQLLSPDVIMIDT MNDRTYDVAAVRERFGVDPEKVPEILGLTGDMSDNIPGIPGIGPKGALRLVEEYGSIEEILRNVERLRNPKLRDSVRVYG EQALLSRDLARIRTGIPLTFDLEEARLQEPDREVLTRLFRELEFSSLLQEFSVVEDCGDNSHTLLRTPEAVAAFMEPLKE GGFLVLEMVLSSSEPMRAELIGVALGLDSGNAGYIPLEPGAAEKSASSLSEEQVFPVLFPFLADKKIALVGHDLKTVLIV LSQKGVKVQNPLLDTMVAAYILNPARRGYELSEIVQDYLHRQVEPLRKKLVGGSSPKQSPASFPPERLRDFACRRVEAIG ELASTLIEKMEETGLRELFDSVEMPLVAVLAAMEEKGVLLDLHLLKEMSQELGHLLSLSEAKIYALAGERFNIASPKQLQ TILFEKLNLPKGKKTKGGYSTDVEVLSNLALNHELPAEILAYRSMAKLKSTYIDALPSLINIRTGRIHTSYNQTVAATGR LSSSNPNLQNIPIRTLEGKRIRQAFIAPPGWAIVSADYSQIELRVLAHLSEDPVLINAFAAGEDIHSRTAADVFGVFPEM INAEMRRQAKVINFGVLYGMSAFGLSRELGIPQKQAQAYIDGYFKKYESVKTYLDGILQQARERGYVTTLLNRRRYLPEI NSVNPAVRQLAERMAINAPIQGTAADLIKMAMLRIADLIREKGMKSAMTMQVHDELVFEVPQEEKIALMNLVRQEMEGVV ELKVPLKVDIASGRNWDEAHS
Sequences:
>Translated_901_residues MSTGDNTENSEKSTIYLVDGSNYLYRAFYAIRELSNSRGLPTNAVYGFTNMLIKLLRERKPEYIAITFDLKGPTFRHETY AQYKATRKATPEALIPQIPYIKDIIRGFSIPILEQQGTEADDIIGTLACRFAGEGLKVIIVSGDKDMLQLLSPDVIMIDT MNDRTYDVAAVRERFGVDPEKVPEILGLTGDMSDNIPGIPGIGPKGALRLVEEYGSIEEILRNVERLRNPKLRDSVRVYG EQALLSRDLARIRTGIPLTFDLEEARLQEPDREVLTRLFRELEFSSLLQEFSVVEDCGDNSHTLLRTPEAVAAFMEPLKE GGFLVLEMVLSSSEPMRAELIGVALGLDSGNAGYIPLEPGAAEKSASSLSEEQVFPVLFPFLADKKIALVGHDLKTVLIV LSQKGVKVQNPLLDTMVAAYILNPARRGYELSEIVQDYLHRQVEPLRKKLVGGSSPKQSPASFPPERLRDFACRRVEAIG ELASTLIEKMEETGLRELFDSVEMPLVAVLAAMEEKGVLLDLHLLKEMSQELGHLLSLSEAKIYALAGERFNIASPKQLQ TILFEKLNLPKGKKTKGGYSTDVEVLSNLALNHELPAEILAYRSMAKLKSTYIDALPSLINIRTGRIHTSYNQTVAATGR LSSSNPNLQNIPIRTLEGKRIRQAFIAPPGWAIVSADYSQIELRVLAHLSEDPVLINAFAAGEDIHSRTAADVFGVFPEM INAEMRRQAKVINFGVLYGMSAFGLSRELGIPQKQAQAYIDGYFKKYESVKTYLDGILQQARERGYVTTLLNRRRYLPEI NSVNPAVRQLAERMAINAPIQGTAADLIKMAMLRIADLIREKGMKSAMTMQVHDELVFEVPQEEKIALMNLVRQEMEGVV ELKVPLKVDIASGRNWDEAHS >Mature_900_residues STGDNTENSEKSTIYLVDGSNYLYRAFYAIRELSNSRGLPTNAVYGFTNMLIKLLRERKPEYIAITFDLKGPTFRHETYA QYKATRKATPEALIPQIPYIKDIIRGFSIPILEQQGTEADDIIGTLACRFAGEGLKVIIVSGDKDMLQLLSPDVIMIDTM NDRTYDVAAVRERFGVDPEKVPEILGLTGDMSDNIPGIPGIGPKGALRLVEEYGSIEEILRNVERLRNPKLRDSVRVYGE QALLSRDLARIRTGIPLTFDLEEARLQEPDREVLTRLFRELEFSSLLQEFSVVEDCGDNSHTLLRTPEAVAAFMEPLKEG GFLVLEMVLSSSEPMRAELIGVALGLDSGNAGYIPLEPGAAEKSASSLSEEQVFPVLFPFLADKKIALVGHDLKTVLIVL SQKGVKVQNPLLDTMVAAYILNPARRGYELSEIVQDYLHRQVEPLRKKLVGGSSPKQSPASFPPERLRDFACRRVEAIGE LASTLIEKMEETGLRELFDSVEMPLVAVLAAMEEKGVLLDLHLLKEMSQELGHLLSLSEAKIYALAGERFNIASPKQLQT ILFEKLNLPKGKKTKGGYSTDVEVLSNLALNHELPAEILAYRSMAKLKSTYIDALPSLINIRTGRIHTSYNQTVAATGRL SSSNPNLQNIPIRTLEGKRIRQAFIAPPGWAIVSADYSQIELRVLAHLSEDPVLINAFAAGEDIHSRTAADVFGVFPEMI NAEMRRQAKVINFGVLYGMSAFGLSRELGIPQKQAQAYIDGYFKKYESVKTYLDGILQQARERGYVTTLLNRRRYLPEIN SVNPAVRQLAERMAINAPIQGTAADLIKMAMLRIADLIREKGMKSAMTMQVHDELVFEVPQEEKIALMNLVRQEMEGVVE LKVPLKVDIASGRNWDEAHS
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=517, Percent_Identity=32.495164410058, Blast_Score=258, Evalue=2e-68, Organism=Homo sapiens, GI139394648, Length=694, Percent_Identity=27.9538904899135, Blast_Score=233, Evalue=6e-61, Organism=Escherichia coli, GI1790294, Length=927, Percent_Identity=41.5318230852211, Blast_Score=663, Evalue=0.0, Organism=Escherichia coli, GI226510970, Length=243, Percent_Identity=32.9218106995885, Blast_Score=139, Evalue=8e-34, Organism=Caenorhabditis elegans, GI86563272, Length=403, Percent_Identity=28.7841191066998, Blast_Score=160, Evalue=3e-39, Organism=Drosophila melanogaster, GI17933644, Length=419, Percent_Identity=31.980906921241, Blast_Score=205, Evalue=1e-52,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR012337 [H]
Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 100318; Mature: 100187
Theoretical pI: Translated: 5.59; Mature: 5.59
Prosite motif: PS00447 DNA_POLYMERASE_A
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSTGDNTENSEKSTIYLVDGSNYLYRAFYAIRELSNSRGLPTNAVYGFTNMLIKLLRERK CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC PEYIAITFDLKGPTFRHETYAQYKATRKATPEALIPQIPYIKDIIRGFSIPILEQQGTEA CCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCCEECCCCCCH DDIIGTLACRFAGEGLKVIIVSGDKDMLQLLSPDVIMIDTMNDRTYDVAAVRERFGVDPE HHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCHH KVPEILGLTGDMSDNIPGIPGIGPKGALRLVEEYGSIEEILRNVERLRNPKLRDSVRVYG HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH EQALLSRDLARIRTGIPLTFDLEEARLQEPDREVLTRLFRELEFSSLLQEFSVVEDCGDN HHHHHHHHHHHHHCCCCEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC SHTLLRTPEAVAAFMEPLKEGGFLVLEMVLSSSEPMRAELIGVALGLDSGNAGYIPLEPG CCEEEECHHHHHHHHHHHHCCCCEEHHHHHCCCCCHHHHHEEEEEECCCCCCCEEECCCC AAEKSASSLSEEQVFPVLFPFLADKKIALVGHDLKTVLIVLSQKGVKVQNPLLDTMVAAY CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHH ILNPARRGYELSEIVQDYLHRQVEPLRKKLVGGSSPKQSPASFPPERLRDFACRRVEAIG HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH ELASTLIEKMEETGLRELFDSVEMPLVAVLAAMEEKGVLLDLHLLKEMSQELGHLLSLSE HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCC AKIYALAGERFNIASPKQLQTILFEKLNLPKGKKTKGGYSTDVEVLSNLALNHELPAEIL CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHH AYRSMAKLKSTYIDALPSLINIRTGRIHTSYNQTVAATGRLSSSNPNLQNIPIRTLEGKR HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCHH IRQAFIAPPGWAIVSADYSQIELRVLAHLSEDPVLINAFAAGEDIHSRTAADVFGVFPEM HHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH INAEMRRQAKVINFGVLYGMSAFGLSRELGIPQKQAQAYIDGYFKKYESVKTYLDGILQQ HHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH ARERGYVTTLLNRRRYLPEINSVNPAVRQLAERMAINAPIQGTAADLIKMAMLRIADLIR HHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH EKGMKSAMTMQVHDELVFEVPQEEKIALMNLVRQEMEGVVELKVPLKVDIASGRNWDEAH HCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCEEEEECCCCCCHHCC S C >Mature Secondary Structure STGDNTENSEKSTIYLVDGSNYLYRAFYAIRELSNSRGLPTNAVYGFTNMLIKLLRERK CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC PEYIAITFDLKGPTFRHETYAQYKATRKATPEALIPQIPYIKDIIRGFSIPILEQQGTEA CCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCCEECCCCCCH DDIIGTLACRFAGEGLKVIIVSGDKDMLQLLSPDVIMIDTMNDRTYDVAAVRERFGVDPE HHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCHH KVPEILGLTGDMSDNIPGIPGIGPKGALRLVEEYGSIEEILRNVERLRNPKLRDSVRVYG HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH EQALLSRDLARIRTGIPLTFDLEEARLQEPDREVLTRLFRELEFSSLLQEFSVVEDCGDN HHHHHHHHHHHHHCCCCEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC SHTLLRTPEAVAAFMEPLKEGGFLVLEMVLSSSEPMRAELIGVALGLDSGNAGYIPLEPG CCEEEECHHHHHHHHHHHHCCCCEEHHHHHCCCCCHHHHHEEEEEECCCCCCCEEECCCC AAEKSASSLSEEQVFPVLFPFLADKKIALVGHDLKTVLIVLSQKGVKVQNPLLDTMVAAY CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHH ILNPARRGYELSEIVQDYLHRQVEPLRKKLVGGSSPKQSPASFPPERLRDFACRRVEAIG HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH ELASTLIEKMEETGLRELFDSVEMPLVAVLAAMEEKGVLLDLHLLKEMSQELGHLLSLSE HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCC AKIYALAGERFNIASPKQLQTILFEKLNLPKGKKTKGGYSTDVEVLSNLALNHELPAEIL CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHH AYRSMAKLKSTYIDALPSLINIRTGRIHTSYNQTVAATGRLSSSNPNLQNIPIRTLEGKR HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCHH IRQAFIAPPGWAIVSADYSQIELRVLAHLSEDPVLINAFAAGEDIHSRTAADVFGVFPEM HHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH INAEMRRQAKVINFGVLYGMSAFGLSRELGIPQKQAQAYIDGYFKKYESVKTYLDGILQQ HHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH ARERGYVTTLLNRRRYLPEINSVNPAVRQLAERMAINAPIQGTAADLIKMAMLRIADLIR HHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH EKGMKSAMTMQVHDELVFEVPQEEKIALMNLVRQEMEGVVELKVPLKVDIASGRNWDEAH HCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCEEEEECCCCCCHHCC S C
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]