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The map label for this gene is dapF

Identifier: 85857916

GI number: 85857916

Start: 73159

End: 73992

Strand: Direct

Name: dapF

Synonym: SYN_00169

Alternate gene names: 85857916

Gene position: 73159-73992 (Clockwise)

Preceding gene: 85857915

Following gene: 85857917

Centisome position: 2.3

GC content: 59.95

Gene sequence:

>834_bases
ATGATCGAATTTTTCAAAATGAGCGGAAGCGGCAATGACTTCATCCTGATAGACAACCGGGAAGGGGCGCTGTCTTCCAT
CAAGAATATTCCGTCCTTCGTGGAGGCGATCTGCCGTCGGAAGGTTTCCGTCGGCGCCGATGGTGTGATCCTCGTCGAAC
GGTCGCCGCGGGCGGATTTCCAGTGGCGCTTTTTCAACGCCGACGGCAGCGAAGTGGAAATGTGCGGCAACGGCGGTCGC
TGTGTCGCCCGCTTTGCCTATCTCAAAGGGATTGCCGGGGAGCGGATGTCCTTTGAAACCGTGGCCGGACTTATCGATGC
CGAACTACGGGGAGACGATGTGAAATTGCGGCTGACCGAACCTTCGTCTCTGCAGGCGAACCAACCCATTCCCGTTGGAG
ACGAAATCCTGACGGTGGACAGCCTCAACACCGGCGTGCCCCACGTCGTGGCCTTCGTCGAGGAGCTGGGCGGATTCGAT
GTTTTCCGTTACGGCCGGGCGTTGCGGTATGCCGAGGCTTTTCAGCCGGCGGGCACGAACGCCAACTTCGTGACCGTCAC
CAGCCTTCACAGCCTTTCCGTCCGGACCTATGAAAGGGGCGTGGAAGACGAAACCCTTGCCTGCGGCACAGGGTCGGTGG
CTTCGGCCCTGGCGGCAGCGGCGCGGGGATTGGTGGAGTCACCGGTGGAGGTGACCGTGAGAAGCGGCGAAAGGCTCAAG
ATTCATTTTGAGCGGATCGACGGTGCGTTCAGGAACGTTTACATGGAAGGCCGGGTCCGGGTGATTTACGAGGGCCGGCT
CTGGGAAGAGGCCTGGCAGGAGAACGGAAATTAA

Upstream 100 bases:

>100_bases
GGGATGAAATGTGAACAGCAGGTCGTTCCGGCGTTGACGCGGGCGTCAGGATGGGTTATGCAGGAGCGAAAAATTTTTCG
AACCGGACAGGAGTTGAAGA

Downstream 100 bases:

>100_bases
ACGATTAAAGGAGAGAGAACGGTGCAGATTGCGGAGCGTTTAGGAAAAATTCCCCCTTACCTGTTCATGGAGTTGCGGAA
AAAGATCAATAAGGCGAAGG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MIEFFKMSGSGNDFILIDNREGALSSIKNIPSFVEAICRRKVSVGADGVILVERSPRADFQWRFFNADGSEVEMCGNGGR
CVARFAYLKGIAGERMSFETVAGLIDAELRGDDVKLRLTEPSSLQANQPIPVGDEILTVDSLNTGVPHVVAFVEELGGFD
VFRYGRALRYAEAFQPAGTNANFVTVTSLHSLSVRTYERGVEDETLACGTGSVASALAAAARGLVESPVEVTVRSGERLK
IHFERIDGAFRNVYMEGRVRVIYEGRLWEEAWQENGN

Sequences:

>Translated_277_residues
MIEFFKMSGSGNDFILIDNREGALSSIKNIPSFVEAICRRKVSVGADGVILVERSPRADFQWRFFNADGSEVEMCGNGGR
CVARFAYLKGIAGERMSFETVAGLIDAELRGDDVKLRLTEPSSLQANQPIPVGDEILTVDSLNTGVPHVVAFVEELGGFD
VFRYGRALRYAEAFQPAGTNANFVTVTSLHSLSVRTYERGVEDETLACGTGSVASALAAAARGLVESPVEVTVRSGERLK
IHFERIDGAFRNVYMEGRVRVIYEGRLWEEAWQENGN
>Mature_277_residues
MIEFFKMSGSGNDFILIDNREGALSSIKNIPSFVEAICRRKVSVGADGVILVERSPRADFQWRFFNADGSEVEMCGNGGR
CVARFAYLKGIAGERMSFETVAGLIDAELRGDDVKLRLTEPSSLQANQPIPVGDEILTVDSLNTGVPHVVAFVEELGGFD
VFRYGRALRYAEAFQPAGTNANFVTVTSLHSLSVRTYERGVEDETLACGTGSVASALAAAARGLVESPVEVTVRSGERLK
IHFERIDGAFRNVYMEGRVRVIYEGRLWEEAWQENGN

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=279, Percent_Identity=32.258064516129, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30384; Mature: 30384

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFFKMSGSGNDFILIDNREGALSSIKNIPSFVEAICRRKVSVGADGVILVERSPRADF
CCEEEEECCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCE
QWRFFNADGSEVEMCGNGGRCVARFAYLKGIAGERMSFETVAGLIDAELRGDDVKLRLTE
EEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
PSSLQANQPIPVGDEILTVDSLNTGVPHVVAFVEELGGFDVFRYGRALRYAEAFQPAGTN
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
ANFVTVTSLHSLSVRTYERGVEDETLACGTGSVASALAAAARGLVESPVEVTVRSGERLK
CCEEEEEEEEHEEHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEE
IHFERIDGAFRNVYMEGRVRVIYEGRLWEEAWQENGN
EEEEHHCCHHHEEEECCEEEEEECCCCHHHHHHCCCC
>Mature Secondary Structure
MIEFFKMSGSGNDFILIDNREGALSSIKNIPSFVEAICRRKVSVGADGVILVERSPRADF
CCEEEEECCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCE
QWRFFNADGSEVEMCGNGGRCVARFAYLKGIAGERMSFETVAGLIDAELRGDDVKLRLTE
EEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
PSSLQANQPIPVGDEILTVDSLNTGVPHVVAFVEELGGFDVFRYGRALRYAEAFQPAGTN
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
ANFVTVTSLHSLSVRTYERGVEDETLACGTGSVASALAAAARGLVESPVEVTVRSGERLK
CCEEEEEEEEHEEHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEE
IHFERIDGAFRNVYMEGRVRVIYEGRLWEEAWQENGN
EEEEHHCCHHHEEEECCEEEEEECCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]