| Definition | Syntrophus aciditrophicus SB chromosome, complete genome. |
|---|---|
| Accession | NC_007759 |
| Length | 3,179,300 |
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The map label for this gene is yitJ [H]
Identifier: 85857910
GI number: 85857910
Start: 66613
End: 68469
Strand: Direct
Name: yitJ [H]
Synonym: SYN_00176
Alternate gene names: 85857910
Gene position: 66613-68469 (Clockwise)
Preceding gene: 85857909
Following gene: 85857911
Centisome position: 2.1
GC content: 60.74
Gene sequence:
>1857_bases ATGAGCGCACTGCAGAATGTCCTTTCCCGCATGACCGGGACCCCGCTGATTTTTGACGGGGCCATGGGGACGATGATTTA TGAAAAAGGGGTCTTTATCCAGACCTGTTACGACGAATTATGCCTGACCGAGCCCGGACTGATTCTGGGGATTCATGAGC AGTACGCTCAGGCCGGGGCGCAGGTGCTGGAAACCAATTCCTTCGGCGCCAACCGGATCAAACTCCGGGCTTACGGCCTG GCGGAAAAAGTGGCCGACATCAACCGCGCCGCAGCAAGACTCGCCCGCCAGGCGGCCGGGACGACGCTCTACGTCGCCGG ATCCGTGGGGCCCTGTACCCAGGGAGGTCAGGTGATCACCGGGAGGGACATGGCGGAAGTCGAAGCCGCCTTCCGGGAAC AGATGGAAGCCCTGACAGAAGAAGGCGTGGACCTGCTGCTTCTGGAGACGTTCAGCGACTTAAAAGAGCTGCAGCTGGCA GCCCGGGTTGCACGTGAAAGAGGCGTGCCGGTGCTGGCCTCCTTTGCCGTGGACGATGATGGGGAGACGGCGGCCGGAAC ACCGGCGGAAAAGATGGCTGCGGCCCTGGAAAAAGATCCTCACGTCGACGTCATCGGCCTCAACTGCGGCACCGGTCCGG CAGGGATTTACGAGGCTCTGCTGAAAGTCCTTCCCGTTGCTGGCAAACCGGTGATCGTGATGCCCAATGCCGGGATGCCG CGGGAAATCGGTGGAAGGACCCTCTATCTGGCCAACCCCGAATATTTCACCGAATATGCCAAAAAATTCATTGAACTGGG CGTTCGCGGAATCGGCGGATGCTGCGGCGTCCATCCCGCACACATCGCCACGGCGGCCCGTGCTGTTCGCGGACTCAGCG GTGTCCGAAAGCATGTCGAAGTGACGACTCGTCAGCAGGCTCCTGCGCAGAAGCCCGCAATTCCGACTGCGGCTAAATCA CGTCTGGCCGCGAAGATATGTTCCGGGCAGAAGGTGACTTCTGTGGAGATTCTTCCCCCCCGGGCCGGCGACTTCAGTAA TATGCTGGCCAAAGTGCGTCGGTGCCATGAAGCCGGCGTCGATGCCATAAACATTCCCGATGGTCCCCGGGCCAGCGCCC GGGTCTCACCCATGATCACGGCCCTGGTCATCCTCAGGGAAGTGGGCATCGAGCCGGTAGTCCATTACTGCTGCCGGGAC CGGAACCTGATCGGGATGCAGAGTGATCTGCTGGGCGGGTACGCCGTGGGGCTCGTGAATTACCTGATCATTACGGGTGA CCCGCCGAAACTGGGAAACTGCCCGGAGGCGACGGGCGTCTTCGATGTGGATTCCATCGGCCTCACCTCCGTGGTCCATA ATCTCAATTCCGGCTGGGACATCGGAGGCAGTCCCGTAGACCCGCCCACGGGCATCTTCATCGGCGTGGGCGCCAATCCC TGCGCGGTGGATCTGGAGCGGGAGATCGACCGCTATTTTCAGAAGATCGAGGCCGGCGCCGAGTTTGCCATTACTCAGCC GGTTTTCGACGAGAAGGCCCTGTTCCGGTTCCTGGACCGCGTGGAGAAGCACTCGAAGCGCGTTCCCGTTCTGGCCGGCG TCTGGCCGCTGTTGAGCTACCGGAATGCGGAATTCATGAATAATGAAGTTCCGGGCGTGATGGTGCCGCGGGAAATCCTC GAGCGCATGGCCCGGTGTGAAACCCGGGAGGAAAGCCAGCGCACCGGGATCGAAATTGCCAGGGCCATCTGTGAAAAAAT TCACGAAAGGGTTGCCGGTTTCCAGGTGTGCGCTCCCTTGAACAACGTGGAAATCGCACTTTCCGTGCTGAGGAAGGGCG AAGGTCGGGAAATCTAG
Upstream 100 bases:
>100_bases TCCGCCATCTCCTGATTCCGTGGAATTTCGGAGAGTTTTTGTTTTACAGGCTGACAAAAAAACGGTATGGAAAAAACGAT GGAATTTTCAAGGAGATGCG
Downstream 100 bases:
>100_bases CCCCGGCATTTTTTATGGAAATATTTTTTCCCCCCTGATATGAGATGAAACTGAGTTTTTAGAGAGGATACATTTCTGTC GGCTCTGGAGTATAGTCGCG
Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein
Products: NA
Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]
Number of amino acids: Translated: 618; Mature: 617
Protein sequence:
>618_residues MSALQNVLSRMTGTPLIFDGAMGTMIYEKGVFIQTCYDELCLTEPGLILGIHEQYAQAGAQVLETNSFGANRIKLRAYGL AEKVADINRAAARLARQAAGTTLYVAGSVGPCTQGGQVITGRDMAEVEAAFREQMEALTEEGVDLLLLETFSDLKELQLA ARVARERGVPVLASFAVDDDGETAAGTPAEKMAAALEKDPHVDVIGLNCGTGPAGIYEALLKVLPVAGKPVIVMPNAGMP REIGGRTLYLANPEYFTEYAKKFIELGVRGIGGCCGVHPAHIATAARAVRGLSGVRKHVEVTTRQQAPAQKPAIPTAAKS RLAAKICSGQKVTSVEILPPRAGDFSNMLAKVRRCHEAGVDAINIPDGPRASARVSPMITALVILREVGIEPVVHYCCRD RNLIGMQSDLLGGYAVGLVNYLIITGDPPKLGNCPEATGVFDVDSIGLTSVVHNLNSGWDIGGSPVDPPTGIFIGVGANP CAVDLEREIDRYFQKIEAGAEFAITQPVFDEKALFRFLDRVEKHSKRVPVLAGVWPLLSYRNAEFMNNEVPGVMVPREIL ERMARCETREESQRTGIEIARAICEKIHERVAGFQVCAPLNNVEIALSVLRKGEGREI
Sequences:
>Translated_618_residues MSALQNVLSRMTGTPLIFDGAMGTMIYEKGVFIQTCYDELCLTEPGLILGIHEQYAQAGAQVLETNSFGANRIKLRAYGL AEKVADINRAAARLARQAAGTTLYVAGSVGPCTQGGQVITGRDMAEVEAAFREQMEALTEEGVDLLLLETFSDLKELQLA ARVARERGVPVLASFAVDDDGETAAGTPAEKMAAALEKDPHVDVIGLNCGTGPAGIYEALLKVLPVAGKPVIVMPNAGMP REIGGRTLYLANPEYFTEYAKKFIELGVRGIGGCCGVHPAHIATAARAVRGLSGVRKHVEVTTRQQAPAQKPAIPTAAKS RLAAKICSGQKVTSVEILPPRAGDFSNMLAKVRRCHEAGVDAINIPDGPRASARVSPMITALVILREVGIEPVVHYCCRD RNLIGMQSDLLGGYAVGLVNYLIITGDPPKLGNCPEATGVFDVDSIGLTSVVHNLNSGWDIGGSPVDPPTGIFIGVGANP CAVDLEREIDRYFQKIEAGAEFAITQPVFDEKALFRFLDRVEKHSKRVPVLAGVWPLLSYRNAEFMNNEVPGVMVPREIL ERMARCETREESQRTGIEIARAICEKIHERVAGFQVCAPLNNVEIALSVLRKGEGREI >Mature_617_residues SALQNVLSRMTGTPLIFDGAMGTMIYEKGVFIQTCYDELCLTEPGLILGIHEQYAQAGAQVLETNSFGANRIKLRAYGLA EKVADINRAAARLARQAAGTTLYVAGSVGPCTQGGQVITGRDMAEVEAAFREQMEALTEEGVDLLLLETFSDLKELQLAA RVARERGVPVLASFAVDDDGETAAGTPAEKMAAALEKDPHVDVIGLNCGTGPAGIYEALLKVLPVAGKPVIVMPNAGMPR EIGGRTLYLANPEYFTEYAKKFIELGVRGIGGCCGVHPAHIATAARAVRGLSGVRKHVEVTTRQQAPAQKPAIPTAAKSR LAAKICSGQKVTSVEILPPRAGDFSNMLAKVRRCHEAGVDAINIPDGPRASARVSPMITALVILREVGIEPVVHYCCRDR NLIGMQSDLLGGYAVGLVNYLIITGDPPKLGNCPEATGVFDVDSIGLTSVVHNLNSGWDIGGSPVDPPTGIFIGVGANPC AVDLEREIDRYFQKIEAGAEFAITQPVFDEKALFRFLDRVEKHSKRVPVLAGVWPLLSYRNAEFMNNEVPGVMVPREILE RMARCETREESQRTGIEIARAICEKIHERVAGFQVCAPLNNVEIALSVLRKGEGREI
Specific function: Terminal step in the de novo biosynthesis of methionine. [C]
COG id: COG0646
COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Hcy-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=385, Percent_Identity=28.051948051948, Blast_Score=117, Evalue=4e-26, Organism=Homo sapiens, GI13162290, Length=304, Percent_Identity=28.2894736842105, Blast_Score=100, Evalue=7e-21, Organism=Homo sapiens, GI157266337, Length=310, Percent_Identity=28.0645161290323, Blast_Score=89, Evalue=1e-17, Organism=Escherichia coli, GI1790450, Length=357, Percent_Identity=28.8515406162465, Blast_Score=112, Evalue=5e-26, Organism=Escherichia coli, GI1786456, Length=289, Percent_Identity=32.1799307958478, Blast_Score=93, Evalue=5e-20, Organism=Caenorhabditis elegans, GI17535405, Length=317, Percent_Identity=30.2839116719243, Blast_Score=121, Evalue=1e-27, Organism=Saccharomyces cerevisiae, GI6322966, Length=323, Percent_Identity=24.1486068111455, Blast_Score=82, Evalue=3e-16,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003171 - InterPro: IPR003726 [H]
Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]
EC number: =2.1.1.10; =1.5.1.20 [H]
Molecular weight: Translated: 66375; Mature: 66244
Theoretical pI: Translated: 6.06; Mature: 6.06
Prosite motif: PS50970 HCY
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.4 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 4.9 %Cys+Met (Translated Protein) 2.4 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 4.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSALQNVLSRMTGTPLIFDGAMGTMIYEKGVFIQTCYDELCLTEPGLILGIHEQYAQAGA CHHHHHHHHHHCCCCEEECCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHH QVLETNSFGANRIKLRAYGLAEKVADINRAAARLARQAAGTTLYVAGSVGPCTQGGQVIT HEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEE GRDMAEVEAAFREQMEALTEEGVDLLLLETFSDLKELQLAARVARERGVPVLASFAVDDD CCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC GETAAGTPAEKMAAALEKDPHVDVIGLNCGTGPAGIYEALLKVLPVAGKPVIVMPNAGMP CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCC REIGGRTLYLANPEYFTEYAKKFIELGVRGIGGCCGVHPAHIATAARAVRGLSGVRKHVE HHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH VTTRQQAPAQKPAIPTAAKSRLAAKICSGQKVTSVEILPPRAGDFSNMLAKVRRCHEAGV HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCC DAINIPDGPRASARVSPMITALVILREVGIEPVVHYCCRDRNLIGMQSDLLGGYAVGLVN CEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHE YLIITGDPPKLGNCPEATGVFDVDSIGLTSVVHNLNSGWDIGGSPVDPPTGIFIGVGANP EEEEECCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCC CAVDLEREIDRYFQKIEAGAEFAITQPVFDEKALFRFLDRVEKHSKRVPVLAGVWPLLSY CEECHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC RNAEFMNNEVPGVMVPREILERMARCETREESQRTGIEIARAICEKIHERVAGFQVCAPL CCCHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC NNVEIALSVLRKGEGREI CCHHHHHHHHHCCCCCCC >Mature Secondary Structure SALQNVLSRMTGTPLIFDGAMGTMIYEKGVFIQTCYDELCLTEPGLILGIHEQYAQAGA HHHHHHHHHHCCCCEEECCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHH QVLETNSFGANRIKLRAYGLAEKVADINRAAARLARQAAGTTLYVAGSVGPCTQGGQVIT HEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEE GRDMAEVEAAFREQMEALTEEGVDLLLLETFSDLKELQLAARVARERGVPVLASFAVDDD CCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC GETAAGTPAEKMAAALEKDPHVDVIGLNCGTGPAGIYEALLKVLPVAGKPVIVMPNAGMP CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCC REIGGRTLYLANPEYFTEYAKKFIELGVRGIGGCCGVHPAHIATAARAVRGLSGVRKHVE HHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH VTTRQQAPAQKPAIPTAAKSRLAAKICSGQKVTSVEILPPRAGDFSNMLAKVRRCHEAGV HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCC DAINIPDGPRASARVSPMITALVILREVGIEPVVHYCCRDRNLIGMQSDLLGGYAVGLVN CEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHE YLIITGDPPKLGNCPEATGVFDVDSIGLTSVVHNLNSGWDIGGSPVDPPTGIFIGVGANP EEEEECCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCC CAVDLEREIDRYFQKIEAGAEFAITQPVFDEKALFRFLDRVEKHSKRVPVLAGVWPLLSY CEECHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC RNAEFMNNEVPGVMVPREILERMARCETREESQRTGIEIARAICEKIHERVAGFQVCAPL CCCHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC NNVEIALSVLRKGEGREI CCHHHHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9353932; 9384377 [H]