Click here to switch to the map view.

The map label for this gene is emrK [H]

Identifier: 85375830

GI number: 85375830

Start: 3032357

End: 3033505

Strand: Reverse

Name: emrK [H]

Synonym: ELI_15015

Alternate gene names: 85375830

Gene position: 3033505-3032357 (Counterclockwise)

Preceding gene: 85375834

Following gene: 85375829

Centisome position: 99.38

GC content: 62.49

Gene sequence:

>1149_bases
ATGGCGAGGAGCGAAATAATCATGGCGGACGAGACAGCAGGGGCTAGCGATGAAGGCGCACGTCCGACGGGGTTGTCCAG
TCCGCGGGTCCGGCTGGGTCTGCTGATCGCCGGCATTCTGGTCCTGCTCGCCGGGAGCTACTGGTATTACAATCATCAGA
CGTACGGCAGATTCCAGCAGTCCACCGACAACGCTTATATTGCCGCCGACAGCGTCATCATCTCCCCCAAAATTGCGGGC
TACGTCGAGCGCGTATTAGTGACGGAGAATCAAACGGTCGCGTCGGGCGATGCACTTGTCCAGCTGGACGTGCGAGATTA
TCGTGCCCAAACGAACCAGGTCGAAGCCCAGATCGCCGCGTCGCTCGCCGGAGCAGATACGGTACGCGCCCAGCAACAAG
AGCAGGACGCGGCAATTGCCCAGGCCCGTGCACAACTCGCAGCGGCATCTACACAGGCTGGTCTCGCTGCTGAGCAGGTT
GTCCGGTACCGCCCCCTTGCCGCTTCGGGAGCGGAGCCGCGCGAGAGGCTCGATCAGCTGGAGGCCCAGGCCAGTGAAGC
GCAGGCTCAAGTCGCGGCAGCGCGCGCCGTGCTCACGGCGGCACAGCGCCGACAGAACACGCTTAGCGAGCAGATAGACC
AGGCCAATTCGCAAGCCAACGCCGCTCGTGCGCAACTCGAGGCCGCCCGTCTGAACCTGTCATCGACCACGCTCCAGGCC
AGTATTTCCGGGCGGGTGGGTGACCTCACGGTCCGACCTGGACAATTTGTCCAGCCTGGCCAACGCCTTATGAGCCTCGT
ACCGACCGACCGCCTCTACGTGACAGCAAATTTCAAGGAAACGCAGCTCGGACTTGTCCGCCCGGGGCAACCCGTCACCT
TGGAAATCGACGCGCTGCCGGACCTCGAGCTATTAGGAAGAGTGGAGAGCATCGCGCCGGGTACCGGTGCGGAATTCTCA
ATCCTTCCGCCACAGAACGCGACGGGTAATTTCACCAAGATCGTTCAGCGCGTCCCGGTGCGCATATCCATTCAGGCTTC
GCCCGAGATTCTGCGTCTGCTGGTGCCGGGCATGTCTGTTGTCGCGAGTGTCGATACGCGCGCCGCCAGAGACGAACTCG
ATTCGATCCGCAACGCGGCCGGAGCTTGA

Upstream 100 bases:

>100_bases
CATCTTCCGTCATGAGACATTCCTAACGATCGGAGAAAAGTAGTAAAGGACTTTCGCATTTTGTTTTGGCCGCTATATCG
CTCGCGCAACGGCTCAAACG

Downstream 100 bases:

>100_bases
CCGATGGCAGGCGCCGCTGCAGGGGTTTCGCCGCCGGTCGAAAAGGCCGATGCTACGGCCTGGATCGCCGTAGCCGCCGG
CGCGCTGGGGGCGATGCTGG

Product: hlyD family multidrug secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MARSEIIMADETAGASDEGARPTGLSSPRVRLGLLIAGILVLLAGSYWYYNHQTYGRFQQSTDNAYIAADSVIISPKIAG
YVERVLVTENQTVASGDALVQLDVRDYRAQTNQVEAQIAASLAGADTVRAQQQEQDAAIAQARAQLAAASTQAGLAAEQV
VRYRPLAASGAEPRERLDQLEAQASEAQAQVAAARAVLTAAQRRQNTLSEQIDQANSQANAARAQLEAARLNLSSTTLQA
SISGRVGDLTVRPGQFVQPGQRLMSLVPTDRLYVTANFKETQLGLVRPGQPVTLEIDALPDLELLGRVESIAPGTGAEFS
ILPPQNATGNFTKIVQRVPVRISIQASPEILRLLVPGMSVVASVDTRAARDELDSIRNAAGA

Sequences:

>Translated_382_residues
MARSEIIMADETAGASDEGARPTGLSSPRVRLGLLIAGILVLLAGSYWYYNHQTYGRFQQSTDNAYIAADSVIISPKIAG
YVERVLVTENQTVASGDALVQLDVRDYRAQTNQVEAQIAASLAGADTVRAQQQEQDAAIAQARAQLAAASTQAGLAAEQV
VRYRPLAASGAEPRERLDQLEAQASEAQAQVAAARAVLTAAQRRQNTLSEQIDQANSQANAARAQLEAARLNLSSTTLQA
SISGRVGDLTVRPGQFVQPGQRLMSLVPTDRLYVTANFKETQLGLVRPGQPVTLEIDALPDLELLGRVESIAPGTGAEFS
ILPPQNATGNFTKIVQRVPVRISIQASPEILRLLVPGMSVVASVDTRAARDELDSIRNAAGA
>Mature_381_residues
ARSEIIMADETAGASDEGARPTGLSSPRVRLGLLIAGILVLLAGSYWYYNHQTYGRFQQSTDNAYIAADSVIISPKIAGY
VERVLVTENQTVASGDALVQLDVRDYRAQTNQVEAQIAASLAGADTVRAQQQEQDAAIAQARAQLAAASTQAGLAAEQVV
RYRPLAASGAEPRERLDQLEAQASEAQAQVAAARAVLTAAQRRQNTLSEQIDQANSQANAARAQLEAARLNLSSTTLQAS
ISGRVGDLTVRPGQFVQPGQRLMSLVPTDRLYVTANFKETQLGLVRPGQPVTLEIDALPDLELLGRVESIAPGTGAEFSI
LPPQNATGNFTKIVQRVPVRISIQASPEILRLLVPGMSVVASVDTRAARDELDSIRNAAGA

Specific function: Unknown

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=343, Percent_Identity=32.9446064139942, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1789041, Length=353, Percent_Identity=32.2946175637394, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790519, Length=315, Percent_Identity=31.1111111111111, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790024, Length=282, Percent_Identity=25.177304964539, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87081951, Length=188, Percent_Identity=34.0425531914894, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789900, Length=348, Percent_Identity=25.5747126436782, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789637, Length=239, Percent_Identity=30.5439330543933, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790012, Length=283, Percent_Identity=25.0883392226148, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40616; Mature: 40485

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSEIIMADETAGASDEGARPTGLSSPRVRLGLLIAGILVLLAGSYWYYNHQTYGRFQQ
CCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHC
STDNAYIAADSVIISPKIAGYVERVLVTENQTVASGDALVQLDVRDYRAQTNQVEAQIAA
CCCCEEEEECCEEECCHHHHHHHHHHEECCCEEECCCCEEEEEHHHHHHHHHHHHHHHHH
SLAGADTVRAQQQEQDAAIAQARAQLAAASTQAGLAAEQVVRYRPLAASGAEPRERLDQL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHH
EAQASEAQAQVAAARAVLTAAQRRQNTLSEQIDQANSQANAARAQLEAARLNLSSTTLQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHEEEE
SISGRVGDLTVRPGQFVQPGQRLMSLVPTDRLYVTANFKETQLGLVRPGQPVTLEIDALP
EECCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCEECEECCCCCEEEEECCCC
DLELLGRVESIAPGTGAEFSILPPQNATGNFTKIVQRVPVRISIQASPEILRLLVPGMSV
CHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEECCHHHHHHHHCCHHH
VASVDTRAARDELDSIRNAAGA
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARSEIIMADETAGASDEGARPTGLSSPRVRLGLLIAGILVLLAGSYWYYNHQTYGRFQQ
CCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHC
STDNAYIAADSVIISPKIAGYVERVLVTENQTVASGDALVQLDVRDYRAQTNQVEAQIAA
CCCCEEEEECCEEECCHHHHHHHHHHEECCCEEECCCCEEEEEHHHHHHHHHHHHHHHHH
SLAGADTVRAQQQEQDAAIAQARAQLAAASTQAGLAAEQVVRYRPLAASGAEPRERLDQL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHH
EAQASEAQAQVAAARAVLTAAQRRQNTLSEQIDQANSQANAARAQLEAARLNLSSTTLQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHEEEE
SISGRVGDLTVRPGQFVQPGQRLMSLVPTDRLYVTANFKETQLGLVRPGQPVTLEIDALP
EECCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCEECEECCCCCEEEEECCCC
DLELLGRVESIAPGTGAEFSILPPQNATGNFTKIVQRVPVRISIQASPEILRLLVPGMSV
CHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEECCHHHHHHHHCCHHH
VASVDTRAARDELDSIRNAAGA
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]