Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 85375829

GI number: 85375829

Start: 3030800

End: 3032353

Strand: Reverse

Name: emrB [H]

Synonym: ELI_15010

Alternate gene names: 85375829

Gene position: 3032353-3030800 (Counterclockwise)

Preceding gene: 85375830

Following gene: 85375828

Centisome position: 99.34

GC content: 59.14

Gene sequence:

>1554_bases
ATGGCAGGCGCCGCTGCAGGGGTTTCGCCGCCGGTCGAAAAGGCCGATGCTACGGCCTGGATCGCCGTAGCCGCCGGCGC
GCTGGGGGCGATGCTGGCAACGCTTGACATCTCGATCGTCAATTCGGCGCTGCCGACGATCCAGGGGGAAATCGGCGCCA
CCGGAACCGAGGGCACCTGGATCGCGACAGCCTTCCTTGTTGCCGAGATCATCATCATCCCTCTGAGCGCGTGGCTGGAA
AGACTGCTCGGCCTTCGCACGCTGCTCATCGTCGCGGTTTCTGCTTTCACGGCCTTTTCGGTGTTGTGCGGCATTGCCAC
CGATCTGACGACGATGATCATCGGTCGCACCGGTCAAGGGTTCATGGGCGGCGCGCTTATTCCAACCGCCATGACCATCG
TCGCAAAGCGACTGCCTCCATCGCAACAGCCGATCGGCATGGCGCTCTTCGGAATGACCGTGGTTCTTGGGCCGGTAATG
GGCCCGTTGGTGGGGGGCTGGCTTACCGAGAACCTGAGCTGGCACTACGCCTTCTTCGTCAACGTGCCCGTCTGCGCGTT
GCTCCTTGCTTTACTATTCATCGGGCTACCCCATGAAAAGCCGGACTGGGTTTACCTGCGCGAGGCTGATTGGGCGGGAA
TAGCCGGTATGATCCTTGGGTTGGGTGGGCTGACCATCGTGCTGGAAGAAGGCCACCGCGAGGAATGGTTCGAATCCACG
CTGATCGTGCAGCTCACTCTGATGACGGTCGTCGGCTTCGCGCTTCTGATCTGCGGGCAGCTGTATGCACGCAAGCCGGT
GCTTAAATTGCGCCTGATGCTCAGCAGGCAGTTTGCCAGTGTCGTGGTGATGGCACTGGCGCTCGGCATGGTCATGTATG
GCTCGACCTACGTGATCCCGCAATTTCTTGCGATAATCTCCGACTACAACGCCTTGCAGACTGGGTTCGTAATCTTCTGG
ATGGGTGTTCCGGCCTTCCTCCTGATGCCTGTGCTGCCCTTCATGATACGAAGGATACACATTCGCATTGCAGTCGGCGT
GGGCATGTTCATGATGGCGCTGAGCTGTTTCATCACTATTGATTTGACGGCAGAATCGGGGGGCGGCGTTTTCACCGAAA
GTCAGTTTCTGCGCGGCATTGGCATGATCCTGACAATGATGTTCCTCAATCAGGCGACCGTTGCTTCGGTTGCGAAGGAG
GATGCCGGAGACGCCTCCGGTATTTTTAATGCGGCGAGAAACCTAGGTGGCTCTTTCGCGCTCGCTGGGCTTGCTTCATT
CCAGGACCAGCGCCTGTGGCATCATAGTCGGCGCATGGAGGAAACTCTCAACGCCAACAGCCCCCGGCTACAGGAATACC
TGGACGGCATGACGGCGACGCTGGGCAGCCCACAGGCCGCGCTGGAGATGCTTTCCGGCATCATTCAGCGCGATGCGTTC
GTGATGACCTACAACGATGTCTTTTTCGCCATGGGCGCGATTACCCTGGTCACGGTGCCGCTGGTACTGTTCCTCAAGCC
TCTCCCGAAGAACGTATCCCTTTCGATGCACTGA

Upstream 100 bases:

>100_bases
GCGTCTGCTGGTGCCGGGCATGTCTGTTGTCGCGAGTGTCGATACGCGCGCCGCCAGAGACGAACTCGATTCGATCCGCA
ACGCGGCCGGAGCTTGACCG

Downstream 100 bases:

>100_bases
GAACTCTATGCGCAAGACCCTAACCCTCCTCGCTCCCATGGCGCTCCTCGCGGGTTGCATGTCCGGTCCCGACTATGCCG
GTCCCCCGCAACTTGCGACC

Product: multidrug resistance transmembrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MAGAAAGVSPPVEKADATAWIAVAAGALGAMLATLDISIVNSALPTIQGEIGATGTEGTWIATAFLVAEIIIIPLSAWLE
RLLGLRTLLIVAVSAFTAFSVLCGIATDLTTMIIGRTGQGFMGGALIPTAMTIVAKRLPPSQQPIGMALFGMTVVLGPVM
GPLVGGWLTENLSWHYAFFVNVPVCALLLALLFIGLPHEKPDWVYLREADWAGIAGMILGLGGLTIVLEEGHREEWFEST
LIVQLTLMTVVGFALLICGQLYARKPVLKLRLMLSRQFASVVVMALALGMVMYGSTYVIPQFLAIISDYNALQTGFVIFW
MGVPAFLLMPVLPFMIRRIHIRIAVGVGMFMMALSCFITIDLTAESGGGVFTESQFLRGIGMILTMMFLNQATVASVAKE
DAGDASGIFNAARNLGGSFALAGLASFQDQRLWHHSRRMEETLNANSPRLQEYLDGMTATLGSPQAALEMLSGIIQRDAF
VMTYNDVFFAMGAITLVTVPLVLFLKPLPKNVSLSMH

Sequences:

>Translated_517_residues
MAGAAAGVSPPVEKADATAWIAVAAGALGAMLATLDISIVNSALPTIQGEIGATGTEGTWIATAFLVAEIIIIPLSAWLE
RLLGLRTLLIVAVSAFTAFSVLCGIATDLTTMIIGRTGQGFMGGALIPTAMTIVAKRLPPSQQPIGMALFGMTVVLGPVM
GPLVGGWLTENLSWHYAFFVNVPVCALLLALLFIGLPHEKPDWVYLREADWAGIAGMILGLGGLTIVLEEGHREEWFEST
LIVQLTLMTVVGFALLICGQLYARKPVLKLRLMLSRQFASVVVMALALGMVMYGSTYVIPQFLAIISDYNALQTGFVIFW
MGVPAFLLMPVLPFMIRRIHIRIAVGVGMFMMALSCFITIDLTAESGGGVFTESQFLRGIGMILTMMFLNQATVASVAKE
DAGDASGIFNAARNLGGSFALAGLASFQDQRLWHHSRRMEETLNANSPRLQEYLDGMTATLGSPQAALEMLSGIIQRDAF
VMTYNDVFFAMGAITLVTVPLVLFLKPLPKNVSLSMH
>Mature_516_residues
AGAAAGVSPPVEKADATAWIAVAAGALGAMLATLDISIVNSALPTIQGEIGATGTEGTWIATAFLVAEIIIIPLSAWLER
LLGLRTLLIVAVSAFTAFSVLCGIATDLTTMIIGRTGQGFMGGALIPTAMTIVAKRLPPSQQPIGMALFGMTVVLGPVMG
PLVGGWLTENLSWHYAFFVNVPVCALLLALLFIGLPHEKPDWVYLREADWAGIAGMILGLGGLTIVLEEGHREEWFESTL
IVQLTLMTVVGFALLICGQLYARKPVLKLRLMLSRQFASVVVMALALGMVMYGSTYVIPQFLAIISDYNALQTGFVIFWM
GVPAFLLMPVLPFMIRRIHIRIAVGVGMFMMALSCFITIDLTAESGGGVFTESQFLRGIGMILTMMFLNQATVASVAKED
AGDASGIFNAARNLGGSFALAGLASFQDQRLWHHSRRMEETLNANSPRLQEYLDGMTATLGSPQAALEMLSGIIQRDAFV
MTYNDVFFAMGAITLVTVPLVLFLKPLPKNVSLSMH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=500, Percent_Identity=29.6, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1788710, Length=508, Percent_Identity=28.740157480315, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87081983, Length=408, Percent_Identity=25.2450980392157, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1790195, Length=412, Percent_Identity=23.3009708737864, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788392, Length=214, Percent_Identity=27.5700934579439, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=169, Percent_Identity=26.6272189349112, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=182, Percent_Identity=21.4285714285714, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=303, Percent_Identity=23.4323432343234, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55384; Mature: 55252

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAAAGVSPPVEKADATAWIAVAAGALGAMLATLDISIVNSALPTIQGEIGATGTEGTW
CCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
IATAFLVAEIIIIPLSAWLERLLGLRTLLIVAVSAFTAFSVLCGIATDLTTMIIGRTGQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FMGGALIPTAMTIVAKRLPPSQQPIGMALFGMTVVLGPVMGPLVGGWLTENLSWHYAFFV
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
NVPVCALLLALLFIGLPHEKPDWVYLREADWAGIAGMILGLGGLTIVLEEGHREEWFEST
CHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHH
LIVQLTLMTVVGFALLICGQLYARKPVLKLRLMLSRQFASVVVMALALGMVMYGSTYVIP
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
QFLAIISDYNALQTGFVIFWMGVPAFLLMPVLPFMIRRIHIRIAVGVGMFMMALSCFITI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
DLTAESGGGVFTESQFLRGIGMILTMMFLNQATVASVAKEDAGDASGIFNAARNLGGSFA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
LAGLASFQDQRLWHHSRRMEETLNANSPRLQEYLDGMTATLGSPQAALEMLSGIIQRDAF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHCCCHHHHHHHHHHHHHCCCE
VMTYNDVFFAMGAITLVTVPLVLFLKPLPKNVSLSMH
EEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AGAAAGVSPPVEKADATAWIAVAAGALGAMLATLDISIVNSALPTIQGEIGATGTEGTW
CCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
IATAFLVAEIIIIPLSAWLERLLGLRTLLIVAVSAFTAFSVLCGIATDLTTMIIGRTGQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FMGGALIPTAMTIVAKRLPPSQQPIGMALFGMTVVLGPVMGPLVGGWLTENLSWHYAFFV
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
NVPVCALLLALLFIGLPHEKPDWVYLREADWAGIAGMILGLGGLTIVLEEGHREEWFEST
CHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHH
LIVQLTLMTVVGFALLICGQLYARKPVLKLRLMLSRQFASVVVMALALGMVMYGSTYVIP
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
QFLAIISDYNALQTGFVIFWMGVPAFLLMPVLPFMIRRIHIRIAVGVGMFMMALSCFITI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
DLTAESGGGVFTESQFLRGIGMILTMMFLNQATVASVAKEDAGDASGIFNAARNLGGSFA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
LAGLASFQDQRLWHHSRRMEETLNANSPRLQEYLDGMTATLGSPQAALEMLSGIIQRDAF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHCCCHHHHHHHHHHHHHCCCE
VMTYNDVFFAMGAITLVTVPLVLFLKPLPKNVSLSMH
EEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]