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The map label for this gene is nodT [H]

Identifier: 85375828

GI number: 85375828

Start: 3029275

End: 3030762

Strand: Reverse

Name: nodT [H]

Synonym: ELI_15005

Alternate gene names: 85375828

Gene position: 3030762-3029275 (Counterclockwise)

Preceding gene: 85375829

Following gene: 85375816

Centisome position: 99.29

GC content: 64.85

Gene sequence:

>1488_bases
ATGGCGCTCCTCGCGGGTTGCATGTCCGGTCCCGACTATGCCGGTCCCCCGCAACTTGCGACCGCCGCAGGCAATGCGTT
CGTACGGGCCGGCCCCGAAATCGATCCGTTAGCGCCGATTGCCGGCGACTGGTGGACGCTGCTCGGCGATCCGGTTCTCA
ATGAACTGGAGGCGCGCGCGCAGGTCGGCAATCCGGGCGTGGCCGCGGCGCGCGCTCGCATCGAACAGGCAAGGGCTTCT
GTCCGCCAGGAGCGCGCCAACCGGCTACCCGCCGTGGCTGCGCAGGCGACTGCGGTCCAGGCGAATATCCCAGGGCTAGA
TATCGGCAGCGGCCCGCCCCCGGGATCGCCCGGCGCTCCTGCAGACACCGAAGAGCAGGACAGCCTCAGCGTCTACAACG
TCGGGCTCAATGCAAACTGGGAGATCGATTTCGCTGGGGGGCAGGCACGGCGTGTCGAGGCCATTAACGCTCAGGCTGCG
GCCTCGGTGGCGAACGCCGAGGATGCGAAAGTCCAGCTCGCCGCCGAGGTCGCGCGGGCGTATGTGAGCCTGCGAGAAGC
GCAGGGGCGACTCGAGCTTGTCCAGCGAGAGCGCGACTTGCAGCAGCAAATTCTCGAACTGACCTATCAGCGCTATACAC
AGGGCGCTCTCGCACTGTTTCCGGTCGGTGACGCGAACGCCGAGCTCGAACTGCTCAAGTCGCAGATCGCCGAAGCTCAA
GCGGACATCGATGTGTTCAAGGACACACTCGCGGTGCTGACGGGCGAAGCGCCCGGCGCACTCGACGGGCTCCTGGCGGC
TCAGGCCGAAATTCCGCTTCCCCCCGAACGGGTCGCGGTGGGCGATCCCGCAGCGCTAATAGCGCGGCGGCCGGATGTTC
GAGCCGCCGAACGCATGCTGGCCGCGGCGAATGCGCGCATTGGCGTGGCAGAGGCTGCTCGCTTCCCCAAGATTTCCTTC
ATGGGGATTCTCGGTTTGGGCGGATCGGAGCCCGACGACATCCTGGACCCGGGCAACTTATCGGCAATCGCTTTGCCGCG
GCTGCAGTGGAACCTGCTTGATTTCGGCCGCACGGCAGCATCGATCGATCAGGCCGAGAGTGCGCGCGACGAGGCCGAAG
CGCGCTATCTTGAAGTGGTCCTGGGCGCACTACGCGACGCCGAACAATCGCTTGCTCGGTTCGGGCAACAAAGAGCCAAC
GTAGCCGCGCTGGCGCAGATCAGTCGGCAGGCCGACACGGCTGCGGAGCTTAACGAGCAACGCCGCGCCGCGGGGGTAAT
CTCGCAAGGCGAACTCAACACTTCGATCCGCAAGCGCGAACAGGCCAGAGCAAATCTCGATCGGGCGATCGCCGCCATGA
CAAATGGCTGGATCGCGATCCAGAAGTCGCTTGGGTTGGGATGGGTCGAATTGTCTCGCGACAATCGCACGTCGGTTGAG
CCAGCTCCGGTCGATCGAAAATCAGTTATGAAACCTGAACAGAGTTAG

Upstream 100 bases:

>100_bases
ACGGTGCCGCTGGTACTGTTCCTCAAGCCTCTCCCGAAGAACGTATCCCTTTCGATGCACTGAGAACTCTATGCGCAAGA
CCCTAACCCTCCTCGCTCCC

Downstream 100 bases:

>100_bases
GATAAGTTTTCTCAGAAGTCTGCCCCGCAGCCCCTTCCAACTTTTCCTAACGCGTCAGCACCTCGTGCACTGTGACCCAA
TCTTGGATCATAGGGGGCAG

Product: putative outer membrane efflux protein OprN precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MALLAGCMSGPDYAGPPQLATAAGNAFVRAGPEIDPLAPIAGDWWTLLGDPVLNELEARAQVGNPGVAAARARIEQARAS
VRQERANRLPAVAAQATAVQANIPGLDIGSGPPPGSPGAPADTEEQDSLSVYNVGLNANWEIDFAGGQARRVEAINAQAA
ASVANAEDAKVQLAAEVARAYVSLREAQGRLELVQRERDLQQQILELTYQRYTQGALALFPVGDANAELELLKSQIAEAQ
ADIDVFKDTLAVLTGEAPGALDGLLAAQAEIPLPPERVAVGDPAALIARRPDVRAAERMLAAANARIGVAEAARFPKISF
MGILGLGGSEPDDILDPGNLSAIALPRLQWNLLDFGRTAASIDQAESARDEAEARYLEVVLGALRDAEQSLARFGQQRAN
VAALAQISRQADTAAELNEQRRAAGVISQGELNTSIRKREQARANLDRAIAAMTNGWIAIQKSLGLGWVELSRDNRTSVE
PAPVDRKSVMKPEQS

Sequences:

>Translated_495_residues
MALLAGCMSGPDYAGPPQLATAAGNAFVRAGPEIDPLAPIAGDWWTLLGDPVLNELEARAQVGNPGVAAARARIEQARAS
VRQERANRLPAVAAQATAVQANIPGLDIGSGPPPGSPGAPADTEEQDSLSVYNVGLNANWEIDFAGGQARRVEAINAQAA
ASVANAEDAKVQLAAEVARAYVSLREAQGRLELVQRERDLQQQILELTYQRYTQGALALFPVGDANAELELLKSQIAEAQ
ADIDVFKDTLAVLTGEAPGALDGLLAAQAEIPLPPERVAVGDPAALIARRPDVRAAERMLAAANARIGVAEAARFPKISF
MGILGLGGSEPDDILDPGNLSAIALPRLQWNLLDFGRTAASIDQAESARDEAEARYLEVVLGALRDAEQSLARFGQQRAN
VAALAQISRQADTAAELNEQRRAAGVISQGELNTSIRKREQARANLDRAIAAMTNGWIAIQKSLGLGWVELSRDNRTSVE
PAPVDRKSVMKPEQS
>Mature_494_residues
ALLAGCMSGPDYAGPPQLATAAGNAFVRAGPEIDPLAPIAGDWWTLLGDPVLNELEARAQVGNPGVAAARARIEQARASV
RQERANRLPAVAAQATAVQANIPGLDIGSGPPPGSPGAPADTEEQDSLSVYNVGLNANWEIDFAGGQARRVEAINAQAAA
SVANAEDAKVQLAAEVARAYVSLREAQGRLELVQRERDLQQQILELTYQRYTQGALALFPVGDANAELELLKSQIAEAQA
DIDVFKDTLAVLTGEAPGALDGLLAAQAEIPLPPERVAVGDPAALIARRPDVRAAERMLAAANARIGVAEAARFPKISFM
GILGLGGSEPDDILDPGNLSAIALPRLQWNLLDFGRTAASIDQAESARDEAEARYLEVVLGALRDAEQSLARFGQQRANV
AALAQISRQADTAAELNEQRRAAGVISQGELNTSIRKREQARANLDRAIAAMTNGWIAIQKSLGLGWVELSRDNRTSVEP
APVDRKSVMKPEQS

Specific function: In Pathogenic Strains It Allows The Bacteria To Invade Brain Microvascular Endothelial Cells (Bmec) Thus Allowing It To Cross The Blood-Brain Barrier And Cause Neonatal Meningitis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=403, Percent_Identity=25.0620347394541, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI2367348, Length=435, Percent_Identity=25.9770114942529, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52294; Mature: 52163

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLAGCMSGPDYAGPPQLATAAGNAFVRAGPEIDPLAPIAGDWWTLLGDPVLNELEARA
CCEEECCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHH
QVGNPGVAAARARIEQARASVRQERANRLPAVAAQATAVQANIPGLDIGSGPPPGSPGAP
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEECCCCCCCCCCCCCCCCCCC
ADTEEQDSLSVYNVGLNANWEIDFAGGQARRVEAINAQAAASVANAEDAKVQLAAEVARA
CCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YVSLREAQGRLELVQRERDLQQQILELTYQRYTQGALALFPVGDANAELELLKSQIAEAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
ADIDVFKDTLAVLTGEAPGALDGLLAAQAEIPLPPERVAVGDPAALIARRPDVRAAERML
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEECCCCHHHHCCCCCHHHHHHHH
AAANARIGVAEAARFPKISFMGILGLGGSEPDDILDPGNLSAIALPRLQWNLLDFGRTAA
HHHCCCCCHHHHHCCCCEEEEEEEECCCCCCHHCCCCCCCCEEEECCCEEHHHHHCCHHH
SIDQAESARDEAEARYLEVVLGALRDAEQSLARFGQQRANVAALAQISRQADTAAELNEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAAGVISQGELNTSIRKREQARANLDRAIAAMTNGWIAIQKSLGLGWVELSRDNRTSVE
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECCCCCCCCC
PAPVDRKSVMKPEQS
CCCCCHHHCCCCCCC
>Mature Secondary Structure 
ALLAGCMSGPDYAGPPQLATAAGNAFVRAGPEIDPLAPIAGDWWTLLGDPVLNELEARA
CEEECCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHH
QVGNPGVAAARARIEQARASVRQERANRLPAVAAQATAVQANIPGLDIGSGPPPGSPGAP
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEECCCCCCCCCCCCCCCCCCC
ADTEEQDSLSVYNVGLNANWEIDFAGGQARRVEAINAQAAASVANAEDAKVQLAAEVARA
CCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YVSLREAQGRLELVQRERDLQQQILELTYQRYTQGALALFPVGDANAELELLKSQIAEAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
ADIDVFKDTLAVLTGEAPGALDGLLAAQAEIPLPPERVAVGDPAALIARRPDVRAAERML
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEECCCCHHHHCCCCCHHHHHHHH
AAANARIGVAEAARFPKISFMGILGLGGSEPDDILDPGNLSAIALPRLQWNLLDFGRTAA
HHHCCCCCHHHHHCCCCEEEEEEEECCCCCCHHCCCCCCCCEEEECCCEEHHHHHCCHHH
SIDQAESARDEAEARYLEVVLGALRDAEQSLARFGQQRANVAALAQISRQADTAAELNEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAAGVISQGELNTSIRKREQARANLDRAIAAMTNGWIAIQKSLGLGWVELSRDNRTSVE
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECCCCCCCCC
PAPVDRKSVMKPEQS
CCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2338917; 2622339 [H]