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The map label for this gene is groEL

Identifier: 85375707

GI number: 85375707

Start: 2896753

End: 2898405

Strand: Reverse

Name: groEL

Synonym: ELI_14400

Alternate gene names: 85375707

Gene position: 2898405-2896753 (Counterclockwise)

Preceding gene: 85375708

Following gene: 85375705

Centisome position: 94.96

GC content: 62.49

Gene sequence:

>1653_bases
ATGGCTGCCAAGGACGTAAAGTTCGGTCGCGACGCTCGCGAAGGCATTCTGAAGGGCGTCGACACGCTCGCCAACGCCGT
CAAGGTCACGCTCGGACCGAAGGGTCGCAATGTCGTGATCGAAAAGAGCTTCGGCGCACCGCGCATCACCAAGGACGGCG
TTACCGTCGCCAAGGAAATCGAGCTCAAGGACAAGTACGAGAACATGGGCGCACAGATGCTGCGCGAAGTGGCCTCGAAG
ACCAACGACCTCGCCGGTGACGGCACCACCACTGCCACCGTGCTCGGACAAGCGATCGTGCGCGAAGGCATGAAGTCGGT
CGCGGCCGGCATGAACCCGATGGACCTCAAGCGCGGCATCGACATCGCCGTCACCAAGGTGGTCGAGAACCTGAAGAGCC
GTTCGAAGGACGTCGCCGGTTCGGAAGAGATCGCTCAGGTCGGCATCATCTCCGCCAATGGCGACCGTGAAGTCGGCGAG
AAGATCGCCGAAGCCATGGAAAAGGTCGGCAAGGAAGGCGTCATCACCGTCGACGAGAGCAAGGGCCTCGAATTCGAGCT
CGAAACCGTCGAAGGCATGCAGTTCGACCGCGGCTACCTTTCGCCCTACTTCATCACCAACCCGGACAAGATGACGGTCG
AACTCGAAAACCCGTACATCCTGATCCACGAGAAGAAGCTCAGCAATCTGCAGGCGATGCTCCCGATCCTCGAAGCGGCT
GTCCAGTCGGGCCGTCCGCTGCTGATTATCGCGGAAGACATCGAAGGCGAAGCGCTGGCGACCCTCGTGGTGAACAAGCT
GCGCGGCGGCCTCAAGGTTGCAGCGGTCAAGGCACCGGGCTTCGGCGACCGCCGCAAGGCCATGCTGCAGGACATCGCGA
TCCTGACCAAGGGGGAAATGATCAGCGAAGATCTCGGCATCAAGCTCGAGAACGTCACGCTGGGCATGCTCGGCGAAGCC
AAGCGCGTCACCATCGACAAGGACAACACGACCATCGTCGACGGTGCCGGTGACGAAGCCGACATCAAGGCCCGCGTGAA
CGAAATCCGCACGCAGATCGACAACACCACCAGCGATTACGACCGCGAGAAGCTGCAAGAGCGCCTCGCCAAGCTCGCCG
GCGGCGTTGCCGTGATCAAGGTCGGCGGTGCTTCGGAAGTCGAAGTGAAGGAACGCAAGGACCGCGTCGACGACGCACTC
CACGCGACCCGTGCTGCGGTCGAAGAAGGCATCGTCCCGGGCGGCGGTACCGCGCTGCTCTACGCTACCAAGGTCCTCGA
AGGGCTGAAGGGCGAGAACGACGACCAGACCCGCGGTATCGACATCGTGCGCAAGGCAATCGTCGCGCCGGTCCGCCAGA
TCGCGACCAATGCCGGTCACGACGGTGCTGTCATCTCGGGCAACCTGCTGCGTGAAGACAACGAATCGCAGGGCTTCAAC
GCTGCGACCGACACCTATGAAGACCTGGTCAAAGCCGGCGTCATCGACCCGACCAAGGTCGTGCGCGTCGCGCTGCAGGA
CGCAGCTTCGGTTGCCGGCCTGCTGATCACCACCGAAGCGGCGATTTCGGAAGTTCCGGAAGACAAGTCGTCCGGTGGCG
GCATGCCCGACATGGGCGGAATGGGCGGAATGGGCGGTATGGGCGGCTTCTAA

Upstream 100 bases:

>100_bases
AAGGCGGGAGCCTCAGGCCACCAGGCCGTACGCTTTCTACCAGGAACACTGAGACCCCAGCCTTCGCTGGGGATCACCAC
GAAGCAAAAGGAACAAACCA

Downstream 100 bases:

>100_bases
GCCCCGCATCGTTCAGACGCATAGAAAGGCCCCGGTGGAGAAATCCGCCGGGGCTTTTTCTATGTCGAATGCGGGCGGGT
CAGGCGCGCGGCAAGCGTCA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 2; Protein Cpn60 2

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MAAKDVKFGRDAREGILKGVDTLANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELKDKYENMGAQMLREVASK
TNDLAGDGTTTATVLGQAIVREGMKSVAAGMNPMDLKRGIDIAVTKVVENLKSRSKDVAGSEEIAQVGIISANGDREVGE
KIAEAMEKVGKEGVITVDESKGLEFELETVEGMQFDRGYLSPYFITNPDKMTVELENPYILIHEKKLSNLQAMLPILEAA
VQSGRPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLQDIAILTKGEMISEDLGIKLENVTLGMLGEA
KRVTIDKDNTTIVDGAGDEADIKARVNEIRTQIDNTTSDYDREKLQERLAKLAGGVAVIKVGGASEVEVKERKDRVDDAL
HATRAAVEEGIVPGGGTALLYATKVLEGLKGENDDQTRGIDIVRKAIVAPVRQIATNAGHDGAVISGNLLREDNESQGFN
AATDTYEDLVKAGVIDPTKVVRVALQDAASVAGLLITTEAAISEVPEDKSSGGGMPDMGGMGGMGGMGGF

Sequences:

>Translated_550_residues
MAAKDVKFGRDAREGILKGVDTLANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELKDKYENMGAQMLREVASK
TNDLAGDGTTTATVLGQAIVREGMKSVAAGMNPMDLKRGIDIAVTKVVENLKSRSKDVAGSEEIAQVGIISANGDREVGE
KIAEAMEKVGKEGVITVDESKGLEFELETVEGMQFDRGYLSPYFITNPDKMTVELENPYILIHEKKLSNLQAMLPILEAA
VQSGRPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLQDIAILTKGEMISEDLGIKLENVTLGMLGEA
KRVTIDKDNTTIVDGAGDEADIKARVNEIRTQIDNTTSDYDREKLQERLAKLAGGVAVIKVGGASEVEVKERKDRVDDAL
HATRAAVEEGIVPGGGTALLYATKVLEGLKGENDDQTRGIDIVRKAIVAPVRQIATNAGHDGAVISGNLLREDNESQGFN
AATDTYEDLVKAGVIDPTKVVRVALQDAASVAGLLITTEAAISEVPEDKSSGGGMPDMGGMGGMGGMGGF
>Mature_549_residues
AAKDVKFGRDAREGILKGVDTLANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELKDKYENMGAQMLREVASKT
NDLAGDGTTTATVLGQAIVREGMKSVAAGMNPMDLKRGIDIAVTKVVENLKSRSKDVAGSEEIAQVGIISANGDREVGEK
IAEAMEKVGKEGVITVDESKGLEFELETVEGMQFDRGYLSPYFITNPDKMTVELENPYILIHEKKLSNLQAMLPILEAAV
QSGRPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLQDIAILTKGEMISEDLGIKLENVTLGMLGEAK
RVTIDKDNTTIVDGAGDEADIKARVNEIRTQIDNTTSDYDREKLQERLAKLAGGVAVIKVGGASEVEVKERKDRVDDALH
ATRAAVEEGIVPGGGTALLYATKVLEGLKGENDDQTRGIDIVRKAIVAPVRQIATNAGHDGAVISGNLLREDNESQGFNA
ATDTYEDLVKAGVIDPTKVVRVALQDAASVAGLLITTEAAISEVPEDKSSGGGMPDMGGMGGMGGMGGF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=528, Percent_Identity=53.5984848484849, Blast_Score=553, Evalue=1e-157,
Organism=Homo sapiens, GI31542947, Length=528, Percent_Identity=53.5984848484849, Blast_Score=553, Evalue=1e-157,
Organism=Escherichia coli, GI1790586, Length=531, Percent_Identity=65.7250470809793, Blast_Score=690, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=54.4423440453686, Blast_Score=554, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI193210679, Length=211, Percent_Identity=52.132701421801, Blast_Score=218, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=59.5419847328244, Blast_Score=609, Evalue=1e-175,
Organism=Saccharomyces cerevisiae, GI6322524, Length=192, Percent_Identity=24.4791666666667, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24641193, Length=529, Percent_Identity=55.0094517958412, Blast_Score=570, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24641191, Length=529, Percent_Identity=55.0094517958412, Blast_Score=570, Evalue=1e-163,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=52, Blast_Score=536, Evalue=1e-152,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=52, Blast_Score=536, Evalue=1e-152,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=52, Blast_Score=536, Evalue=1e-152,
Organism=Drosophila melanogaster, GI17864606, Length=554, Percent_Identity=46.028880866426, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24584129, Length=527, Percent_Identity=38.3301707779886, Blast_Score=331, Evalue=7e-91,
Organism=Drosophila melanogaster, GI19921262, Length=527, Percent_Identity=38.3301707779886, Blast_Score=331, Evalue=7e-91,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH602_ERYLH (Q2N5R9)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459769.1
- HSSP:   P0A6F5
- ProteinModelPortal:   Q2N5R9
- SMR:   Q2N5R9
- STRING:   Q2N5R9
- GeneID:   3869184
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_14400
- NMPDR:   fig|314225.3.peg.139
- eggNOG:   COG0459
- HOGENOM:   HBG625289
- OMA:   NKPETAT
- PhylomeDB:   Q2N5R9
- ProtClustDB:   PRK12849
- BioCyc:   ELIT314225:ELI_14400-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 58248; Mature: 58117

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDVKFGRDAREGILKGVDTLANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEI
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCCCCCCEEHHH
ELKDKYENMGAQMLREVASKTNDLAGDGTTTATVLGQAIVREGMKSVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
DIAVTKVVENLKSRSKDVAGSEEIAQVGIISANGDREVGEKIAEAMEKVGKEGVITVDES
HHHHHHHHHHHHHHCCCCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECC
KGLEFELETVEGMQFDRGYLSPYFITNPDKMTVELENPYILIHEKKLSNLQAMLPILEAA
CCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHH
VQSGRPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLQDIAILTKGEM
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCH
ISEDLGIKLENVTLGMLGEAKRVTIDKDNTTIVDGAGDEADIKARVNEIRTQIDNTTSDY
HHHHHCCEEEEEEEEECCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHH
DREKLQERLAKLAGGVAVIKVGGASEVEVKERKDRVDDALHATRAAVEEGIVPGGGTALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
YATKVLEGLKGENDDQTRGIDIVRKAIVAPVRQIATNAGHDGAVISGNLLREDNESQGFN
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCC
AATDTYEDLVKAGVIDPTKVVRVALQDAASVAGLLITTEAAISEVPEDKSSGGGMPDMGG
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECHHHHHHCCCCCCCCCCCCCCCC
MGGMGGMGGF
CCCCCCCCCC
>Mature Secondary Structure 
AAKDVKFGRDAREGILKGVDTLANAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEI
CCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCCCCCCEEHHH
ELKDKYENMGAQMLREVASKTNDLAGDGTTTATVLGQAIVREGMKSVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
DIAVTKVVENLKSRSKDVAGSEEIAQVGIISANGDREVGEKIAEAMEKVGKEGVITVDES
HHHHHHHHHHHHHHCCCCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECC
KGLEFELETVEGMQFDRGYLSPYFITNPDKMTVELENPYILIHEKKLSNLQAMLPILEAA
CCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHH
VQSGRPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLQDIAILTKGEM
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCH
ISEDLGIKLENVTLGMLGEAKRVTIDKDNTTIVDGAGDEADIKARVNEIRTQIDNTTSDY
HHHHHCCEEEEEEEEECCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHH
DREKLQERLAKLAGGVAVIKVGGASEVEVKERKDRVDDALHATRAAVEEGIVPGGGTALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
YATKVLEGLKGENDDQTRGIDIVRKAIVAPVRQIATNAGHDGAVISGNLLREDNESQGFN
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCC
AATDTYEDLVKAGVIDPTKVVRVALQDAASVAGLLITTEAAISEVPEDKSSGGGMPDMGG
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECHHHHHHCCCCCCCCCCCCCCCC
MGGMGGMGGF
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA