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The map label for this gene is bioF [H]

Identifier: 85375642

GI number: 85375642

Start: 2830993

End: 2832243

Strand: Reverse

Name: bioF [H]

Synonym: ELI_14075

Alternate gene names: 85375642

Gene position: 2832243-2830993 (Counterclockwise)

Preceding gene: 85375643

Following gene: 85375641

Centisome position: 92.79

GC content: 62.11

Gene sequence:

>1251_bases
ATGAGCGAAGGCATTTCGCAGCCCGACCAGCCGGAAGATCTCGGCGGCGGGGACACGGACCTGTTCAGCAAGTTCGACGA
TGTTATCGAACTGCGCAAGGGCCTGCTCGCCAGCGGGCAGGAGGATCCGTTCAACCTGGTGATGGAGAAGGTCCTGTCGC
CGACCCGCGCGATCTGCAACGGGCGCGACACCATCCTGCTCGGCACCTACAATTACATGGGCATGACTTTCGACCCGGAC
GTAATCGAAGCGGGCGAGCAGGCGCTGCGCGATTACGGCACCGGCACCACCGGCAGCCGCGTGCTCAACGGCACATATCA
GGGTCACAAGGAGTGCGAGGAAGCGCTTAAAGAGTTTTACGGGATGGACCATGCCATGGTCTTCTCGACCGGCTACCTCG
CCAATCTCGGGATCATTTCGACCATCGCCGGCAAGGGCGATTACATCATCCTCGACATCGATAGCCACGCCAGCATCTGG
GATGGCTGTGCGATGGGCAATGCCGAAGTCGTGCCCTTCAAGCACAACGATGTCGAAGCGCTGGAAAAGCGCCTGAAGCG
CGTACCCGAAGGGGCTGGCAAGCTGGTGATCCTCGAAGGCGTCTATTCGATGCTGGGCGATGTCGCGCCGCTGCGCGAGA
TGGTAGCTGTCGCCAAGAAACACGGCGCCATGGTGCTGGTCGACGAAGCGCATTCGATGGGGTTTATCGGGGAGAACGGG
CGCGGGGTCTGCGAAGCGGCGGGCGTGATCGACGATTGCGATTTCATCATAGGCACCTTTTCCAAGAGCGTCGGCACCGT
CGGCGGTTTCTGCGTGTCCAACCACCCGAAGTTCGAGATCATGCGGCTGGTTTGCCGGCCTTATGTCTTCACCGCATCGC
TCCCGCCGAGCGTGGTGGCGACCGCTGCAACGTCGATCCGCAAGCTGATGCACGGCTCCAACAAGCGCGCGCATCTGTGG
GAGAACTCGCGCAACCTCCATCTCGGCCTGCAGGAGCTCGGCTTCCAGCTCGGCACCGACGCGCCGGAAAGCGCGATCAT
CGCCGTCATCATGCCCGACCTCGAAAAGGGCGCGGCGATGTGGGAAGCGCTGCTGAAGGAAGGACTCTACGTCAATTTGG
CCCGTCCGCCGGCCACGCCCGCCAACATGACCCTGCTGCGCTGCTCGCTCTGCGCCGAGCATAGCGAAGAGGAAGTGACG
ACGATCCTCGGCATGTTCGAACGGGCGGGGAAGACGGTCGGGATTATCTGA

Upstream 100 bases:

>100_bases
GGGCTTCGACAAGCTCAGCCCGAACGGATAGAGGGGCTGAAAGATCACGGACAGGGAGCCGCGTGAAGGTGTCGTTCTCG
AAAATTACGGAAGCAGCAGC

Downstream 100 bases:

>100_bases
TCGGAGAAGCAGCTGGGGAACGCTCGCACCGGTCTTGCCGTTGGATGTCTTGAAACAGGAGATCGACGATGAAGACCACC
AACACCGGCAGCGCAACCTA

Product: serine palmitoyltransferase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSEGISQPDQPEDLGGGDTDLFSKFDDVIELRKGLLASGQEDPFNLVMEKVLSPTRAICNGRDTILLGTYNYMGMTFDPD
VIEAGEQALRDYGTGTTGSRVLNGTYQGHKECEEALKEFYGMDHAMVFSTGYLANLGIISTIAGKGDYIILDIDSHASIW
DGCAMGNAEVVPFKHNDVEALEKRLKRVPEGAGKLVILEGVYSMLGDVAPLREMVAVAKKHGAMVLVDEAHSMGFIGENG
RGVCEAAGVIDDCDFIIGTFSKSVGTVGGFCVSNHPKFEIMRLVCRPYVFTASLPPSVVATAATSIRKLMHGSNKRAHLW
ENSRNLHLGLQELGFQLGTDAPESAIIAVIMPDLEKGAAMWEALLKEGLYVNLARPPATPANMTLLRCSLCAEHSEEEVT
TILGMFERAGKTVGII

Sequences:

>Translated_416_residues
MSEGISQPDQPEDLGGGDTDLFSKFDDVIELRKGLLASGQEDPFNLVMEKVLSPTRAICNGRDTILLGTYNYMGMTFDPD
VIEAGEQALRDYGTGTTGSRVLNGTYQGHKECEEALKEFYGMDHAMVFSTGYLANLGIISTIAGKGDYIILDIDSHASIW
DGCAMGNAEVVPFKHNDVEALEKRLKRVPEGAGKLVILEGVYSMLGDVAPLREMVAVAKKHGAMVLVDEAHSMGFIGENG
RGVCEAAGVIDDCDFIIGTFSKSVGTVGGFCVSNHPKFEIMRLVCRPYVFTASLPPSVVATAATSIRKLMHGSNKRAHLW
ENSRNLHLGLQELGFQLGTDAPESAIIAVIMPDLEKGAAMWEALLKEGLYVNLARPPATPANMTLLRCSLCAEHSEEEVT
TILGMFERAGKTVGII
>Mature_415_residues
SEGISQPDQPEDLGGGDTDLFSKFDDVIELRKGLLASGQEDPFNLVMEKVLSPTRAICNGRDTILLGTYNYMGMTFDPDV
IEAGEQALRDYGTGTTGSRVLNGTYQGHKECEEALKEFYGMDHAMVFSTGYLANLGIISTIAGKGDYIILDIDSHASIWD
GCAMGNAEVVPFKHNDVEALEKRLKRVPEGAGKLVILEGVYSMLGDVAPLREMVAVAKKHGAMVLVDEAHSMGFIGENGR
GVCEAAGVIDDCDFIIGTFSKSVGTVGGFCVSNHPKFEIMRLVCRPYVFTASLPPSVVATAATSIRKLMHGSNKRAHLWE
NSRNLHLGLQELGFQLGTDAPESAIIAVIMPDLEKGAAMWEALLKEGLYVNLARPPATPANMTLLRCSLCAEHSEEEVTT
ILGMFERAGKTVGII

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=348, Percent_Identity=31.8965517241379, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI284448556, Length=348, Percent_Identity=31.8965517241379, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI83977442, Length=365, Percent_Identity=30.958904109589, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI83977440, Length=365, Percent_Identity=30.958904109589, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI83977444, Length=358, Percent_Identity=31.2849162011173, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI119220554, Length=367, Percent_Identity=31.6076294277929, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI4758668, Length=366, Percent_Identity=32.7868852459016, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI4502025, Length=349, Percent_Identity=32.0916905444126, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI40316939, Length=349, Percent_Identity=32.0916905444126, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI5454084, Length=287, Percent_Identity=30.3135888501742, Blast_Score=152, Evalue=6e-37,
Organism=Escherichia coli, GI1790046, Length=362, Percent_Identity=34.8066298342541, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1786993, Length=348, Percent_Identity=30.4597701149425, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71994529, Length=347, Percent_Identity=33.7175792507205, Blast_Score=218, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI32566772, Length=369, Percent_Identity=29.810298102981, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI71982625, Length=256, Percent_Identity=30.859375, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982617, Length=256, Percent_Identity=30.859375, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560914, Length=364, Percent_Identity=27.7472527472527, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17560912, Length=364, Percent_Identity=27.7472527472527, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320267, Length=366, Percent_Identity=29.7814207650273, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320438, Length=352, Percent_Identity=28.4090909090909, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6323954, Length=297, Percent_Identity=24.9158249158249, Blast_Score=116, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24662918, Length=390, Percent_Identity=29.2307692307692, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI24653280, Length=283, Percent_Identity=33.9222614840989, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24653276, Length=283, Percent_Identity=33.9222614840989, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24653278, Length=283, Percent_Identity=33.9222614840989, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI17137420, Length=350, Percent_Identity=30, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17136286, Length=367, Percent_Identity=27.5204359673025, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 44896; Mature: 44765

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGISQPDQPEDLGGGDTDLFSKFDDVIELRKGLLASGQEDPFNLVMEKVLSPTRAICN
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHC
GRDTILLGTYNYMGMTFDPDVIEAGEQALRDYGTGTTGSRVLNGTYQGHKECEEALKEFY
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHH
GMDHAMVFSTGYLANLGIISTIAGKGDYIILDIDSHASIWDGCAMGNAEVVPFKHNDVEA
CCCCEEEEECCHHHHCCHHHHCCCCCCEEEEEECCCCCHHHHHCCCCCEEEEECCCCHHH
LEKRLKRVPEGAGKLVILEGVYSMLGDVAPLREMVAVAKKHGAMVLVDEAHSMGFIGENG
HHHHHHHCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
RGVCEAAGVIDDCDFIIGTFSKSVGTVGGFCVSNHPKFEIMRLVCRPYVFTASLPPSVVA
CCHHHHCCCHHCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHCCEEEECCCCHHHHH
TAATSIRKLMHGSNKRAHLWENSRNLHLGLQELGFQLGTDAPESAIIAVIMPDLEKGAAM
HHHHHHHHHHCCCCCCEEEECCCCCEEECHHHHCHHCCCCCCCCCEEEEEECCCHHHHHH
WEALLKEGLYVNLARPPATPANMTLLRCSLCAEHSEEEVTTILGMFERAGKTVGII
HHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCC
>Mature Secondary Structure 
SEGISQPDQPEDLGGGDTDLFSKFDDVIELRKGLLASGQEDPFNLVMEKVLSPTRAICN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHC
GRDTILLGTYNYMGMTFDPDVIEAGEQALRDYGTGTTGSRVLNGTYQGHKECEEALKEFY
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHH
GMDHAMVFSTGYLANLGIISTIAGKGDYIILDIDSHASIWDGCAMGNAEVVPFKHNDVEA
CCCCEEEEECCHHHHCCHHHHCCCCCCEEEEEECCCCCHHHHHCCCCCEEEEECCCCHHH
LEKRLKRVPEGAGKLVILEGVYSMLGDVAPLREMVAVAKKHGAMVLVDEAHSMGFIGENG
HHHHHHHCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
RGVCEAAGVIDDCDFIIGTFSKSVGTVGGFCVSNHPKFEIMRLVCRPYVFTASLPPSVVA
CCHHHHCCCHHCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHCCEEEECCCCHHHHH
TAATSIRKLMHGSNKRAHLWENSRNLHLGLQELGFQLGTDAPESAIIAVIMPDLEKGAAM
HHHHHHHHHHCCCCCCEEEECCCCCEEECHHHHCHHCCCCCCCCCEEEEEECCCHHHHHH
WEALLKEGLYVNLARPPATPANMTLLRCSLCAEHSEEEVTTILGMFERAGKTVGII
HHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA