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The map label for this gene is alkJ [H]

Identifier: 85375603

GI number: 85375603

Start: 2797727

End: 2799334

Strand: Reverse

Name: alkJ [H]

Synonym: ELI_13880

Alternate gene names: 85375603

Gene position: 2799334-2797727 (Counterclockwise)

Preceding gene: 85375604

Following gene: 85375593

Centisome position: 91.71

GC content: 63.99

Gene sequence:

>1608_bases
GTGAATCAATACGACTACATCGTGATCGGCGGCGGCAGTGCAGGCAGCGCGGTGGCCGGACGGCTGGCCGTCGACGGCAC
GCGGCAGGTCTGCCTGCTCGAAGCGGGCGGGCGCAACAACAACATGCTGGTCAAGACGCCCGGCTTCATGCCGTTCCTGC
TCAAGAACACCAATTACCGCTACGACACCGTGCCGCAGAAGGGGCTCAATGGCCGGATCGGCTACCAGCCGCGCGGGAAG
GGGCTAGGCGGATCATCGGCGATCAACGCCATGGTCTATATCCGCGGGCACAGATGGGATTACGACAATTGGGCCGCGAT
GGGCTGCGATGGCTGGTCCTATGATGACGTGTTGCCCTGGTTCAAGAAAGCCGAGGCCAACGAGCGCGGGGCGGACGAGT
ATCACGGGGCCGGCGGACCACTGTTCGTCAGCGACCAGAAATACGCCAATCCCACAAGCCATGCCTTCATCGAAGCTGCG
GCGCAGCTCCAGCTACCGACCAACGCGGATTTCAACGGCGCCAAGCAGGAAGGCTTCGGCCTCTACCAGGTGACCCAACG
CAATGGCGAACGCTGGTCCGCAGCGCGCGCCTATATCGAACCGATCCGCGAGGCGCCCAATCTGGATATCCGCACCAGGA
CGCTGGTCGAGCACCTGATTATCGATGGCGGCAAGGTCACCGGCGTCGCGATCAAGCGCGGCGGACTCATCGGAAGCAAG
CGCGAGATCCTGACCGCGCGCAAGGGGGTGATCCTCTCTGCCGGCGCGTTCAATTCACCGCAGATCCTGATGCTGTCGGG
GATAGGCCCGGGCGACCATCTCAGGGAACACGGCATCGCAGTGAAGATCGACAAGCCCGCGGTCGGGAGCGAGTTGCAGG
ACCACATCGATTACGTCTCCGGCTGGGCGACCAAGAGCACGGTGCCGATCGGCGATTCGCTCGAGGGGACCGCGCGGATG
GCGAAAGCGATCATAGAGCACCGCCGCCTGCGTACCGGGATCATGACCACGCCCTATGCCGAGGCCGGCGGGTTCTGGAA
GGTCATGCCCGATGCGCCGTCACCCGATGTGCAATGGCATTTCGTCCCGGCTGTCCTCGAAGACCATGGTCGCGAGAAAG
TGAAAGGCCACGGCTTCTCGCTTCATGCCTGCGTGCTGCGCCCGGAAAGCCGCGGCACGGTTCGGCTCAATTCGGCCGAT
CCGGCGGAGGGTCCGCGCATCGATCCCAACTTCCTCGACGACGATCGCGACATCGCCGTGATGCGCGAAGGCGTGCGCCT
GTCGCATCGGATCGTCGAAGGGGCCGCGATGCAGGCCTATGAGCCGACCGACCGGCATCCCATCGACCTAAACGACGATG
CCGCGCTCGACGAGTTGATCCGCAGCCGCGCCGACACGGTCTACCATCCCGTCGGCACCTGCCGGATGGGCGCGGACGAA
GATGCCGTTGTCGACACCAAGCTCAAGGCGCGCGGTGTCGAAGGGCTTTGGATCGCCGATGCCTCGATCATGCCCAAGAT
CGTCAGCGGCAACACCAATGCGCCCAGCATCATGATCGGCGAACGCTGTGCCGATTTCGTGATGGCCGCAGAGAAGCAAT
TCGCCTGA

Upstream 100 bases:

>100_bases
TGGCGCGCGCGCACGGCCAACGAGCCGTCCGATACCGCCGAAAAGCCGATTGCCGGAACAGAGCAGGCTGCGTAACGCTT
TCCTTCAAGAGGAGAGCATC

Downstream 100 bases:

>100_bases
TTGCGACTAGCGACAAGAAAGGGGCCGGGAAGCAGATGCTGCCCGGCCCCTTCTTTGTGTGTCGCCGCGGTCAGAACGTC
TGGCGCGCCGTGATCCCGAA

Product: dehydrogenase (polyethylene glycol dehydrogenase, alcoholdehydrogenase, L-sorbose dehydrogenase)

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MNQYDYIVIGGGSAGSAVAGRLAVDGTRQVCLLEAGGRNNNMLVKTPGFMPFLLKNTNYRYDTVPQKGLNGRIGYQPRGK
GLGGSSAINAMVYIRGHRWDYDNWAAMGCDGWSYDDVLPWFKKAEANERGADEYHGAGGPLFVSDQKYANPTSHAFIEAA
AQLQLPTNADFNGAKQEGFGLYQVTQRNGERWSAARAYIEPIREAPNLDIRTRTLVEHLIIDGGKVTGVAIKRGGLIGSK
REILTARKGVILSAGAFNSPQILMLSGIGPGDHLREHGIAVKIDKPAVGSELQDHIDYVSGWATKSTVPIGDSLEGTARM
AKAIIEHRRLRTGIMTTPYAEAGGFWKVMPDAPSPDVQWHFVPAVLEDHGREKVKGHGFSLHACVLRPESRGTVRLNSAD
PAEGPRIDPNFLDDDRDIAVMREGVRLSHRIVEGAAMQAYEPTDRHPIDLNDDAALDELIRSRADTVYHPVGTCRMGADE
DAVVDTKLKARGVEGLWIADASIMPKIVSGNTNAPSIMIGERCADFVMAAEKQFA

Sequences:

>Translated_535_residues
MNQYDYIVIGGGSAGSAVAGRLAVDGTRQVCLLEAGGRNNNMLVKTPGFMPFLLKNTNYRYDTVPQKGLNGRIGYQPRGK
GLGGSSAINAMVYIRGHRWDYDNWAAMGCDGWSYDDVLPWFKKAEANERGADEYHGAGGPLFVSDQKYANPTSHAFIEAA
AQLQLPTNADFNGAKQEGFGLYQVTQRNGERWSAARAYIEPIREAPNLDIRTRTLVEHLIIDGGKVTGVAIKRGGLIGSK
REILTARKGVILSAGAFNSPQILMLSGIGPGDHLREHGIAVKIDKPAVGSELQDHIDYVSGWATKSTVPIGDSLEGTARM
AKAIIEHRRLRTGIMTTPYAEAGGFWKVMPDAPSPDVQWHFVPAVLEDHGREKVKGHGFSLHACVLRPESRGTVRLNSAD
PAEGPRIDPNFLDDDRDIAVMREGVRLSHRIVEGAAMQAYEPTDRHPIDLNDDAALDELIRSRADTVYHPVGTCRMGADE
DAVVDTKLKARGVEGLWIADASIMPKIVSGNTNAPSIMIGERCADFVMAAEKQFA
>Mature_535_residues
MNQYDYIVIGGGSAGSAVAGRLAVDGTRQVCLLEAGGRNNNMLVKTPGFMPFLLKNTNYRYDTVPQKGLNGRIGYQPRGK
GLGGSSAINAMVYIRGHRWDYDNWAAMGCDGWSYDDVLPWFKKAEANERGADEYHGAGGPLFVSDQKYANPTSHAFIEAA
AQLQLPTNADFNGAKQEGFGLYQVTQRNGERWSAARAYIEPIREAPNLDIRTRTLVEHLIIDGGKVTGVAIKRGGLIGSK
REILTARKGVILSAGAFNSPQILMLSGIGPGDHLREHGIAVKIDKPAVGSELQDHIDYVSGWATKSTVPIGDSLEGTARM
AKAIIEHRRLRTGIMTTPYAEAGGFWKVMPDAPSPDVQWHFVPAVLEDHGREKVKGHGFSLHACVLRPESRGTVRLNSAD
PAEGPRIDPNFLDDDRDIAVMREGVRLSHRIVEGAAMQAYEPTDRHPIDLNDDAALDELIRSRADTVYHPVGTCRMGADE
DAVVDTKLKARGVEGLWIADASIMPKIVSGNTNAPSIMIGERCADFVMAAEKQFA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=538, Percent_Identity=40.7063197026022, Blast_Score=386, Evalue=1e-107,
Organism=Escherichia coli, GI1786503, Length=537, Percent_Identity=40.5959031657356, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17532301, Length=546, Percent_Identity=37.9120879120879, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI45549471, Length=569, Percent_Identity=37.4340949033392, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI24642042, Length=571, Percent_Identity=36.9527145359019, Blast_Score=334, Evalue=7e-92,
Organism=Drosophila melanogaster, GI45551458, Length=569, Percent_Identity=37.4340949033392, Blast_Score=334, Evalue=8e-92,
Organism=Drosophila melanogaster, GI24642059, Length=575, Percent_Identity=37.5652173913043, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24642055, Length=577, Percent_Identity=37.261698440208, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI17137792, Length=561, Percent_Identity=36.3636363636364, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24642048, Length=581, Percent_Identity=35.6282271944923, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24642039, Length=574, Percent_Identity=35.7142857142857, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24642037, Length=573, Percent_Identity=36.4746945898778, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24650267, Length=577, Percent_Identity=34.4887348353553, Blast_Score=278, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24642035, Length=573, Percent_Identity=35.7766143106457, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI18859995, Length=573, Percent_Identity=34.9040139616056, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24642051, Length=573, Percent_Identity=34.5549738219895, Blast_Score=268, Evalue=7e-72,
Organism=Drosophila melanogaster, GI18859993, Length=571, Percent_Identity=33.6252189141856, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=28.1786941580756, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24642057, Length=586, Percent_Identity=26.1092150170648, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58198; Mature: 58198

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQYDYIVIGGGSAGSAVAGRLAVDGTRQVCLLEAGGRNNNMLVKTPGFMPFLLKNTNYR
CCCCCEEEEECCCCCCHHCCEEEECCCCEEEEEECCCCCCCEEEECCCCCEEEEECCCCE
YDTVPQKGLNGRIGYQPRGKGLGGSSAINAMVYIRGHRWDYDNWAAMGCDGWSYDDVLPW
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEECCCCCCHHHHHHH
FKKAEANERGADEYHGAGGPLFVSDQKYANPTSHAFIEAAAQLQLPTNADFNGAKQEGFG
HHHCCCCCCCCHHHCCCCCCEEECCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCC
LYQVTQRNGERWSAARAYIEPIREAPNLDIRTRTLVEHLIIDGGKVTGVAIKRGGLIGSK
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
REILTARKGVILSAGAFNSPQILMLSGIGPGDHLREHGIAVKIDKPAVGSELQDHIDYVS
HHHHHHCCCEEEECCCCCCCCEEEEECCCCHHHHHHCCEEEEECCCCCCHHHHHHHHHHH
GWATKSTVPIGDSLEGTARMAKAIIEHRRLRTGIMTTPYAEAGGFWKVMPDAPSPDVQWH
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEE
FVPAVLEDHGREKVKGHGFSLHACVLRPESRGTVRLNSADPAEGPRIDPNFLDDDRDIAV
EEHHHHHHCCCHHHCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHH
MREGVRLSHRIVEGAAMQAYEPTDRHPIDLNDDAALDELIRSRADTVYHPVGTCRMGADE
HHHCHHHHHHHHHCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCEECCCCC
DAVVDTKLKARGVEGLWIADASIMPKIVSGNTNAPSIMIGERCADFVMAAEKQFA
CCEEECCHHHCCCCEEEEECCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQYDYIVIGGGSAGSAVAGRLAVDGTRQVCLLEAGGRNNNMLVKTPGFMPFLLKNTNYR
CCCCCEEEEECCCCCCHHCCEEEECCCCEEEEEECCCCCCCEEEECCCCCEEEEECCCCE
YDTVPQKGLNGRIGYQPRGKGLGGSSAINAMVYIRGHRWDYDNWAAMGCDGWSYDDVLPW
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEECCCCCCHHHHHHH
FKKAEANERGADEYHGAGGPLFVSDQKYANPTSHAFIEAAAQLQLPTNADFNGAKQEGFG
HHHCCCCCCCCHHHCCCCCCEEECCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCC
LYQVTQRNGERWSAARAYIEPIREAPNLDIRTRTLVEHLIIDGGKVTGVAIKRGGLIGSK
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
REILTARKGVILSAGAFNSPQILMLSGIGPGDHLREHGIAVKIDKPAVGSELQDHIDYVS
HHHHHHCCCEEEECCCCCCCCEEEEECCCCHHHHHHCCEEEEECCCCCCHHHHHHHHHHH
GWATKSTVPIGDSLEGTARMAKAIIEHRRLRTGIMTTPYAEAGGFWKVMPDAPSPDVQWH
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEE
FVPAVLEDHGREKVKGHGFSLHACVLRPESRGTVRLNSADPAEGPRIDPNFLDDDRDIAV
EEHHHHHHCCCHHHCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHH
MREGVRLSHRIVEGAAMQAYEPTDRHPIDLNDDAALDELIRSRADTVYHPVGTCRMGADE
HHHCHHHHHHHHHCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCEECCCCC
DAVVDTKLKARGVEGLWIADASIMPKIVSGNTNAPSIMIGERCADFVMAAEKQFA
CCEEECCHHHCCCCEEEEECCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]