Click here to switch to the map view.

The map label for this gene is purK [H]

Identifier: 85375542

GI number: 85375542

Start: 2735998

End: 2737053

Strand: Reverse

Name: purK [H]

Synonym: ELI_13575

Alternate gene names: 85375542

Gene position: 2737053-2735998 (Counterclockwise)

Preceding gene: 85375543

Following gene: 85375541

Centisome position: 89.67

GC content: 65.25

Gene sequence:

>1056_bases
ATGATCAAGCCGGGGGGGACAATCGGGATATTGGGCGGCGGCCAACTCGGCCGGATGATGGCGATCGCCGCTGCGCAGAT
GGGCTATCGCTGCATCGCCTACACGCCCGAGAGCGAGCACGTGGTCGGCGATGTCTGCCACGACGTCTTCACCAACAAGT
GGGACGATGCCCAAGCGATGGCGGCCTTCGCGCAGAAGTGCGATATCGTCACGTGGGAATTCGAGAATGTCCCGGTCGGC
CCGCTGGCACCCATCGAAGACCGCCTTGCCCCGCATCCGCGCGCTCTGGAAACCGCGCAGGACCGCCTGAACGAGAAACG
CTTCGTCGAAGGGCTGGGCGGCCGGCCTGCGCCCTATGCCACGGTCGATTCGCGCGCCGAGCTGATGCAGGCGGTCGAGC
GGATCGGTACGCCGGGCATCCTCAAGACTCGCCGCGACGGCTATGACGGCAAGGGCCAATGGCGCATCACGTCCGAGCGC
GACGCAGAGGGCGTGTCGCTGCCCGATGTGCCGTGCATTTACGAAGGCTTCGTCCGGTTCGAGGCAGAATTCTCCGTCAT
CCTCGCTCGCACCGCAGCGCGCGAGGTGGCGTTCTGGGACAGCACCGTCAATGTCCACGAAGGCGGGATGCTGGCGACCT
CCACGCTGCCCCCAGGCGATCTGATCGAGGGCGAGGTCGCCGAGGCGCGCGAGCTGGCGAAACAGGTTGCCGAAGCGCTC
AATTATGTCGGTGTGCTGACACTCGAATTCTTCGCCACCAAGCAAGGCCCTGTCTTCAACGAAATGGCCCCGCGAGTGCA
TAATTCAGGCCACTGGACCATCGAAGGCGCCGCCACGAGCCAGTTCGAAAACCACATCCGAGCGGTCTGCGGCCTGCCGC
TGGGCGACTCCCGCACCGTCGCGCCGGGCGTGGTGATGACCAATATCGTCGGCAAAGATGCCAACGAGGCCGCGCACTGG
CTCGACGATCCGGCGACGCACCTGCACCTCTACGGCAAGACCAAGGCACGGAAGGGCCGCAAGATGGGGCATGCGACCAA
GCTGGTCTGGGACTGA

Upstream 100 bases:

>100_bases
CTGCGAGTATCCTCGCTACATCGGACGACGCGCTGGCCGTGCGCTTGAAGGCGTGGCGCGCCGCACGCAGCGAGGCTGTC
GCAGAGAAGCCGGAATAGCC

Downstream 100 bases:

>100_bases
CGCATTGAGCGACCTGTCCCAGCCTTTCATGATCTATGCCCGCGCCGCCAATGGCACGATCGGCAAGGACGGCGGCATGC
CCTGGCACTTGCCGACCGAC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MIKPGGTIGILGGGQLGRMMAIAAAQMGYRCIAYTPESEHVVGDVCHDVFTNKWDDAQAMAAFAQKCDIVTWEFENVPVG
PLAPIEDRLAPHPRALETAQDRLNEKRFVEGLGGRPAPYATVDSRAELMQAVERIGTPGILKTRRDGYDGKGQWRITSER
DAEGVSLPDVPCIYEGFVRFEAEFSVILARTAAREVAFWDSTVNVHEGGMLATSTLPPGDLIEGEVAEARELAKQVAEAL
NYVGVLTLEFFATKQGPVFNEMAPRVHNSGHWTIEGAATSQFENHIRAVCGLPLGDSRTVAPGVVMTNIVGKDANEAAHW
LDDPATHLHLYGKTKARKGRKMGHATKLVWD

Sequences:

>Translated_351_residues
MIKPGGTIGILGGGQLGRMMAIAAAQMGYRCIAYTPESEHVVGDVCHDVFTNKWDDAQAMAAFAQKCDIVTWEFENVPVG
PLAPIEDRLAPHPRALETAQDRLNEKRFVEGLGGRPAPYATVDSRAELMQAVERIGTPGILKTRRDGYDGKGQWRITSER
DAEGVSLPDVPCIYEGFVRFEAEFSVILARTAAREVAFWDSTVNVHEGGMLATSTLPPGDLIEGEVAEARELAKQVAEAL
NYVGVLTLEFFATKQGPVFNEMAPRVHNSGHWTIEGAATSQFENHIRAVCGLPLGDSRTVAPGVVMTNIVGKDANEAAHW
LDDPATHLHLYGKTKARKGRKMGHATKLVWD
>Mature_351_residues
MIKPGGTIGILGGGQLGRMMAIAAAQMGYRCIAYTPESEHVVGDVCHDVFTNKWDDAQAMAAFAQKCDIVTWEFENVPVG
PLAPIEDRLAPHPRALETAQDRLNEKRFVEGLGGRPAPYATVDSRAELMQAVERIGTPGILKTRRDGYDGKGQWRITSER
DAEGVSLPDVPCIYEGFVRFEAEFSVILARTAAREVAFWDSTVNVHEGGMLATSTLPPGDLIEGEVAEARELAKQVAEAL
NYVGVLTLEFFATKQGPVFNEMAPRVHNSGHWTIEGAATSQFENHIRAVCGLPLGDSRTVAPGVVMTNIVGKDANEAAHW
LDDPATHLHLYGKTKARKGRKMGHATKLVWD

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=342, Percent_Identity=36.5497076023392, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1788155, Length=363, Percent_Identity=25.6198347107438, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324702, Length=368, Percent_Identity=28.804347826087, Blast_Score=146, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 38216; Mature: 38216

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPGGTIGILGGGQLGRMMAIAAAQMGYRCIAYTPESEHVVGDVCHDVFTNKWDDAQAM
CCCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHH
AAFAQKCDIVTWEFENVPVGPLAPIEDRLAPHPRALETAQDRLNEKRFVEGLGGRPAPYA
HHHHHHCCEEEEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
TVDSRAELMQAVERIGTPGILKTRRDGYDGKGQWRITSERDAEGVSLPDVPCIYEGFVRF
CHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
EAEFSVILARTAAREVAFWDSTVNVHEGGMLATSTLPPGDLIEGEVAEARELAKQVAEAL
HHHHEEEEHHHHHHHEEEECCCCEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
NYVGVLTLEFFATKQGPVFNEMAPRVHNSGHWTIEGAATSQFENHIRAVCGLPLGDSRTV
HHHHHEEEEEEECCCCCCHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCC
APGVVMTNIVGKDANEAAHWLDDPATHLHLYGKTKARKGRKMGHATKLVWD
CCCHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCHHHCCCCCCCCCCEECC
>Mature Secondary Structure
MIKPGGTIGILGGGQLGRMMAIAAAQMGYRCIAYTPESEHVVGDVCHDVFTNKWDDAQAM
CCCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHH
AAFAQKCDIVTWEFENVPVGPLAPIEDRLAPHPRALETAQDRLNEKRFVEGLGGRPAPYA
HHHHHHCCEEEEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
TVDSRAELMQAVERIGTPGILKTRRDGYDGKGQWRITSERDAEGVSLPDVPCIYEGFVRF
CHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
EAEFSVILARTAAREVAFWDSTVNVHEGGMLATSTLPPGDLIEGEVAEARELAKQVAEAL
HHHHEEEEHHHHHHHEEEECCCCEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
NYVGVLTLEFFATKQGPVFNEMAPRVHNSGHWTIEGAATSQFENHIRAVCGLPLGDSRTV
HHHHHEEEEEEECCCCCCHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCC
APGVVMTNIVGKDANEAAHWLDDPATHLHLYGKTKARKGRKMGHATKLVWD
CCCHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCHHHCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]