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The map label for this gene is dhfrIII [H]

Identifier: 85375541

GI number: 85375541

Start: 2735499

End: 2735969

Strand: Reverse

Name: dhfrIII [H]

Synonym: ELI_13570

Alternate gene names: 85375541

Gene position: 2735969-2735499 (Counterclockwise)

Preceding gene: 85375542

Following gene: 85375540

Centisome position: 89.63

GC content: 65.18

Gene sequence:

>471_bases
ATGATCTATGCCCGCGCCGCCAATGGCACGATCGGCAAGGACGGCGGCATGCCCTGGCACTTGCCGACCGACCTCAAGCG
CTTCAAGCGGCTGACCACCGGCCATGTCCTGATCATGGGCCGCAAGACCTTCGAGAGCTTTCCCGACCCGCTGCCCGGTC
GCCGCCATATCGTGCTGACGCGCGACGAGGACTGGGGGGCGGACGGCGCAGAAGTGGCGCATTCGCGCGCCGAAGCGCTG
CAACTGGCGGGCGAGGAGCCGGTCGCCATCATCGGCGGCGCGGAGGTTTATGCGCTGTTCGAGCAGGAAGCCGAACGCAT
CGAGCTGACCCAGATTCACGCGAAATACGAAGGCGACACCTTCATGGACGCGCCCGGCCCCGACTGGCGCGAGATCGCGC
GTGACGAATACGAGGCCGAAGATGGTCGACCTGCCTTCTCTTTCGTCACCTTTCTGAGAGCAGAGCGATGA

Upstream 100 bases:

>100_bases
CACCTCTACGGCAAGACCAAGGCACGGAAGGGCCGCAAGATGGGGCATGCGACCAAGCTGGTCTGGGACTGACGCATTGA
GCGACCTGTCCCAGCCTTTC

Downstream 100 bases:

>100_bases
AAAAGCGCTACTGGATCCCGCTGCTGGTGCTCGCGATCGCCGCCAGTGCCTTCTTCGCCTTCGCCCCCGGCTTTGCCGAG
CGGGACATGAACCGGATCGA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MIYARAANGTIGKDGGMPWHLPTDLKRFKRLTTGHVLIMGRKTFESFPDPLPGRRHIVLTRDEDWGADGAEVAHSRAEAL
QLAGEEPVAIIGGAEVYALFEQEAERIELTQIHAKYEGDTFMDAPGPDWREIARDEYEAEDGRPAFSFVTFLRAER

Sequences:

>Translated_156_residues
MIYARAANGTIGKDGGMPWHLPTDLKRFKRLTTGHVLIMGRKTFESFPDPLPGRRHIVLTRDEDWGADGAEVAHSRAEAL
QLAGEEPVAIIGGAEVYALFEQEAERIELTQIHAKYEGDTFMDAPGPDWREIARDEYEAEDGRPAFSFVTFLRAER
>Mature_156_residues
MIYARAANGTIGKDGGMPWHLPTDLKRFKRLTTGHVLIMGRKTFESFPDPLPGRRHIVLTRDEDWGADGAEVAHSRAEAL
QLAGEEPVAIIGGAEVYALFEQEAERIELTQIHAKYEGDTFMDAPGPDWREIARDEYEAEDGRPAFSFVTFLRAER

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=150, Percent_Identity=38, Blast_Score=95, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17505899, Length=144, Percent_Identity=34.7222222222222, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI116008026, Length=153, Percent_Identity=32.6797385620915, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24647458, Length=153, Percent_Identity=32.6797385620915, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17514; Mature: 17514

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYARAANGTIGKDGGMPWHLPTDLKRFKRLTTGHVLIMGRKTFESFPDPLPGRRHIVLT
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCEEEEEE
RDEDWGADGAEVAHSRAEALQLAGEEPVAIIGGAEVYALFEQEAERIELTQIHAKYEGDT
ECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHEEEEEEEEEECCCE
FMDAPGPDWREIARDEYEAEDGRPAFSFVTFLRAER
EECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIYARAANGTIGKDGGMPWHLPTDLKRFKRLTTGHVLIMGRKTFESFPDPLPGRRHIVLT
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCEEEEEE
RDEDWGADGAEVAHSRAEALQLAGEEPVAIIGGAEVYALFEQEAERIELTQIHAKYEGDT
ECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHEEEEEEEEEECCCE
FMDAPGPDWREIARDEYEAEDGRPAFSFVTFLRAER
EECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]