Click here to switch to the map view.

The map label for this gene is algA [H]

Identifier: 85375513

GI number: 85375513

Start: 2706721

End: 2707743

Strand: Reverse

Name: algA [H]

Synonym: ELI_13430

Alternate gene names: 85375513

Gene position: 2707743-2706721 (Counterclockwise)

Preceding gene: 85375517

Following gene: 85375512

Centisome position: 88.71

GC content: 66.18

Gene sequence:

>1023_bases
ATGAGCGCTCTGATCCATCCGGTGATCCTCTGCGGCGGTGGCGGCACGCGATTGTGGCCGCGCAGTCGGCCCGAGCGGCC
CAAACCATTTCTACACCTGCTGGGCGAGCGGACCTTGTTCCAGCAAACGCTCGATCGGGTGGCGGATGGAAACCGCTTTG
CGCTGCCAACAATTGTCGCGGGCGAAAGTCACATGCCGCTCATTCGCGAACAGGCGGGCGATACGCCCGATATCCACCTG
ATCGCGGAACCCATGGGGCGCAACACCGCACCGGCGATCGCGCTCGCGGCCCACCGCTTGCCGGAGGAGGCGATCATGCT
GGTCTGCCCGAGCGACCATTTCATCGCCGACACCGCCGCCTTCCGCGACGCGGTCGAAAGCGCTGCCGATCTGGCGAGCG
AGGGCTATCTCGTCAGCCTCGGCATCACGCCCGATCGCCCGGAAACCGGCTATGGCTACATCAAGCGCGGCGCGGCGCTC
GCGGGCGGCTTTGCGGTCGAACGTTTCGTGGAGAAACCCGACCAGCCGACGGCCGAACGGTTTCTCGCCGATGGCGGCTA
TAGCTGGAACGGCGGCATTTTCCTCTTTTCCGCTGCGCGCCTGCTCGGCGAATTATCGCGCCATCGCCCCGAAATGGCGG
AACTGGTCGAGCGTGCAGCGGCGCATGGAAACGAGGCCACTCGCATATTCAGGCCCGAAGCGGCGAGCTTTGCGGCGATC
GAGGGCGAGTCGATCGATTATGCGCTTATGGAGCCGACCGATCGCGCCGCCATGGTGCCGGTAAGCATGGGATGGTCCGA
CATCGGAAACTGGCAGGCGCTGGCCGACGCGCGTGGCGGACGGTCGCAGGGGCCATGCGACCTGCTCGATGCACGCAACG
TCCACGTCGACAGCGACGGACCGCGTGTGTCCGTGGTGGGCCTCAGCGACGTCATCGTGGTGGTCGATGGCGGCGAAGTG
CTGGTGACCAGCGCCGCGGGCGCACAGAAGGTCGGCAAACTCCCCGGAGCCATGGGCGAATGA

Upstream 100 bases:

>100_bases
GACTCTTCTGATTGCATCCCTCGCGGCGCCGCCTACAGTGCCGCGCTGACTACGGCAATCGCTCACATTTTCTTGAGGCA
TTCATGCTACGGGCCGCACC

Downstream 100 bases:

>100_bases
GCGCGCTCCTCTCTACACACCGGGTGGAGAAAGTCTGGGGGCGCGAGCATCTGCCGCCGCCCTTTACCAGCGAAAGCGAT
AATCGCGTCGGCGAAATCTG

Product: putative GDP-mannose pyrophosphorylase

Products: NA

Alternate protein names: Mannose-6-phosphate isomerase; Phosphohexomutase; Phosphomannose isomerase; PMI; Mannose-1-phosphate guanylyltransferase; GDP-mannose pyrophosphorylase; GMP; GMPP; GTP--mannose-1-phosphate guanylyltransferase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSALIHPVILCGGGGTRLWPRSRPERPKPFLHLLGERTLFQQTLDRVADGNRFALPTIVAGESHMPLIREQAGDTPDIHL
IAEPMGRNTAPAIALAAHRLPEEAIMLVCPSDHFIADTAAFRDAVESAADLASEGYLVSLGITPDRPETGYGYIKRGAAL
AGGFAVERFVEKPDQPTAERFLADGGYSWNGGIFLFSAARLLGELSRHRPEMAELVERAAAHGNEATRIFRPEAASFAAI
EGESIDYALMEPTDRAAMVPVSMGWSDIGNWQALADARGGRSQGPCDLLDARNVHVDSDGPRVSVVGLSDVIVVVDGGEV
LVTSAAGAQKVGKLPGAMGE

Sequences:

>Translated_340_residues
MSALIHPVILCGGGGTRLWPRSRPERPKPFLHLLGERTLFQQTLDRVADGNRFALPTIVAGESHMPLIREQAGDTPDIHL
IAEPMGRNTAPAIALAAHRLPEEAIMLVCPSDHFIADTAAFRDAVESAADLASEGYLVSLGITPDRPETGYGYIKRGAAL
AGGFAVERFVEKPDQPTAERFLADGGYSWNGGIFLFSAARLLGELSRHRPEMAELVERAAAHGNEATRIFRPEAASFAAI
EGESIDYALMEPTDRAAMVPVSMGWSDIGNWQALADARGGRSQGPCDLLDARNVHVDSDGPRVSVVGLSDVIVVVDGGEV
LVTSAAGAQKVGKLPGAMGE
>Mature_339_residues
SALIHPVILCGGGGTRLWPRSRPERPKPFLHLLGERTLFQQTLDRVADGNRFALPTIVAGESHMPLIREQAGDTPDIHLI
AEPMGRNTAPAIALAAHRLPEEAIMLVCPSDHFIADTAAFRDAVESAADLASEGYLVSLGITPDRPETGYGYIKRGAALA
GGFAVERFVEKPDQPTAERFLADGGYSWNGGIFLFSAARLLGELSRHRPEMAELVERAAAHGNEATRIFRPEAASFAAIE
GESIDYALMEPTDRAAMVPVSMGWSDIGNWQALADARGGRSQGPCDLLDARNVHVDSDGPRVSVVGLSDVIVVVDGGEVL
VTSAAGAQKVGKLPGAMGE

Specific function: Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=347, Percent_Identity=40.3458213256484, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =5.3.1.8; =2.7.7.13 [H]

Molecular weight: Translated: 36177; Mature: 36046

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALIHPVILCGGGGTRLWPRSRPERPKPFLHLLGERTLFQQTLDRVADGNRFALPTIVA
CCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCEEECCEEEE
GESHMPLIREQAGDTPDIHLIAEPMGRNTAPAIALAAHRLPEEAIMLVCPSDHFIADTAA
CCCCCCHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCHHHHH
FRDAVESAADLASEGYLVSLGITPDRPETGYGYIKRGAALAGGFAVERFVEKPDQPTAER
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHH
FLADGGYSWNGGIFLFSAARLLGELSRHRPEMAELVERAAAHGNEATRIFRPEAASFAAI
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCEEEE
EGESIDYALMEPTDRAAMVPVSMGWSDIGNWQALADARGGRSQGPCDLLDARNVHVDSDG
CCCCCCEEEECCCCCEEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCHHCCCCEEECCCC
PRVSVVGLSDVIVVVDGGEVLVTSAAGAQKVGKLPGAMGE
CEEEEEECCEEEEEEECCCEEEEECCCHHHHHCCCCCCCC
>Mature Secondary Structure 
SALIHPVILCGGGGTRLWPRSRPERPKPFLHLLGERTLFQQTLDRVADGNRFALPTIVA
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCEEECCEEEE
GESHMPLIREQAGDTPDIHLIAEPMGRNTAPAIALAAHRLPEEAIMLVCPSDHFIADTAA
CCCCCCHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCHHHHH
FRDAVESAADLASEGYLVSLGITPDRPETGYGYIKRGAALAGGFAVERFVEKPDQPTAER
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHH
FLADGGYSWNGGIFLFSAARLLGELSRHRPEMAELVERAAAHGNEATRIFRPEAASFAAI
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCEEEE
EGESIDYALMEPTDRAAMVPVSMGWSDIGNWQALADARGGRSQGPCDLLDARNVHVDSDG
CCCCCCEEEECCCCCEEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCHHCCCCEEECCCC
PRVSVVGLSDVIVVVDGGEVLVTSAAGAQKVGKLPGAMGE
CEEEEEECCEEEEEEECCCEEEEECCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]