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The map label for this gene is lig [H]

Identifier: 85375460

GI number: 85375460

Start: 2649695

End: 2651290

Strand: Reverse

Name: lig [H]

Synonym: ELI_13165

Alternate gene names: 85375460

Gene position: 2651290-2649695 (Counterclockwise)

Preceding gene: 85375461

Following gene: 85375454

Centisome position: 86.86

GC content: 64.47

Gene sequence:

>1596_bases
GTGCAAGCCTTCGCCGACCTGATCGACGCGCTGGTCTATACGCGCAGCCGCAACGAGAAATTGCGGCTTATTGCCGCATA
TTTGCGTGAAACGCCCGACCCGGACCGCGGCTGGGCGCTGGCGGCACTGACCGACGGGCTCGATTTCCCGGCGGTCAAGT
CGAGCGCGATCCGCACGATCATGAAGGAGCGGATCGATCCGGTCCTGTGGACACTCAGCCGCGATTTCGTGGGCGATACG
GCGGAAACCGCGAGCCTGCTCTGGCCCGCCCCCGATAGTGACGTCACGCCGCCTCCCTCGGTGAGCGAAGTGGTTGATCT
ACTGTCGAAAATGACGCGCCAATCGGTGCTCACCGAGCTGCCCGCGCTGCTCGACAGGCTCGATCCGTCGGGCCGGTACG
CGCTTCTAAAGATCGCAACGGGCGCGATGCGGATCGGCGTGTCCAGCCGTTTGGCCAAGGTCGCTTTCGCGCAAGCCTTC
GGCGTCGATGTCGAAGATGTCGAGGAGTATTGGCACGGGCTCGAGCCGCCTTACGAGGAGCTGTTCGCCTGGGCCGCCGA
CGGGGCCGACCCGCCCGATACCGAGAACAAGCCGACCTTCCGCCCCTTCATGCTTGCGCATCCGCTCGAGGACACGGTGG
TCGACATGGCGGACTATGCTGCCGAGTGGAAATGGGACGGCATCCGCGTCCAGCTCGTCCATGCCGGTGGCGAGACGCGG
ATTTATTCGCGCAGCGGCGACGATATCAGCCGCACCTTCCCCGAAATGGTCGACGCGCTGCAGGTCCCGGCGGTGCTGGA
CGGCGAGCTGCTGGTGCGCGGCGATGCACAGGGCGGTGAAGCGGGCGGCGCTGCAAGTTTCAACGCGCTGCAGCAACGGC
TGGGGCGCAAGACGGTCAGCAAGAAGATGCTGAGCGAAAGCCCTGCCTTCGTGCGGCTCTACGACGCGCTGATCATCGAA
GGCGAAGACCTGCGCGACCAACCGTGGGAGAGGCGCAGAGACCATCTCGAACGCTTCGTGCCGCGCCTGCCGGACAGCCA
TTTCGACCTCAGCACCATCGTCGATGCGCGTGATTTCGAGCACCTGGCCGAAATCCGCGAAGGCACGCGCGAGGACGCGA
TCGAGGGCCTGATGCTCAAGCGCCGCGACAGCCCCTATATCGCCGGACGCAAGACCGGGCTGTGGTACAAATGGAAGCGC
GATCCGCTGCTGGTGGATTGCGTGCTGATGTATGCCCAGCGCGGCAGCGGAAAGCGCTCGAGCTTCTATTCCGATTACAC
TTTCGGCTGCTGGGACGGCGACCCCGACAAGGGGGCCGAGCTATTGCCGGTGGGCAAAGCCTATTCGGGTTTCACCGACG
AGGAGCTCAAGAAACTGGATCGGCTGGTGCGCCAGAACACCCTCAACCGCTTCGGTCCGGTGCGCGAGGTCGAACGCACG
CTCGTTTTCGAAGTCGCCTTCGACAGCGTGCATACCAGCAAGCGTCACAAGTCCGGCCTCGCCATGCGGTTCCCGCGGAT
TCACCGCATCCGCTGGGACAAGCCGGTGCACGAAGCGGACCGGCTCCAGACGCTCAAGGCCATGATCCAGGACTAA

Upstream 100 bases:

>100_bases
GCTGAGCCTGTCGAAGCCCAAGCCTTTTTTTTGGCAATCGGGGCGCTTGAAGAAAGTACGGCCCTTCGACAGGCTCAGGG
CGAACGGGGTTTTTGAAGCT

Downstream 100 bases:

>100_bases
GCGAAAGTCGCGCCGCGCGACTTTGCGTCAGTTGCCAGTCGCTTTCACCGCAGCCTTATGCGCGCGACCATTTTCGACAT
AATGCGCCACGCCCATGCGC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MQAFADLIDALVYTRSRNEKLRLIAAYLRETPDPDRGWALAALTDGLDFPAVKSSAIRTIMKERIDPVLWTLSRDFVGDT
AETASLLWPAPDSDVTPPPSVSEVVDLLSKMTRQSVLTELPALLDRLDPSGRYALLKIATGAMRIGVSSRLAKVAFAQAF
GVDVEDVEEYWHGLEPPYEELFAWAADGADPPDTENKPTFRPFMLAHPLEDTVVDMADYAAEWKWDGIRVQLVHAGGETR
IYSRSGDDISRTFPEMVDALQVPAVLDGELLVRGDAQGGEAGGAASFNALQQRLGRKTVSKKMLSESPAFVRLYDALIIE
GEDLRDQPWERRRDHLERFVPRLPDSHFDLSTIVDARDFEHLAEIREGTREDAIEGLMLKRRDSPYIAGRKTGLWYKWKR
DPLLVDCVLMYAQRGSGKRSSFYSDYTFGCWDGDPDKGAELLPVGKAYSGFTDEELKKLDRLVRQNTLNRFGPVREVERT
LVFEVAFDSVHTSKRHKSGLAMRFPRIHRIRWDKPVHEADRLQTLKAMIQD

Sequences:

>Translated_531_residues
MQAFADLIDALVYTRSRNEKLRLIAAYLRETPDPDRGWALAALTDGLDFPAVKSSAIRTIMKERIDPVLWTLSRDFVGDT
AETASLLWPAPDSDVTPPPSVSEVVDLLSKMTRQSVLTELPALLDRLDPSGRYALLKIATGAMRIGVSSRLAKVAFAQAF
GVDVEDVEEYWHGLEPPYEELFAWAADGADPPDTENKPTFRPFMLAHPLEDTVVDMADYAAEWKWDGIRVQLVHAGGETR
IYSRSGDDISRTFPEMVDALQVPAVLDGELLVRGDAQGGEAGGAASFNALQQRLGRKTVSKKMLSESPAFVRLYDALIIE
GEDLRDQPWERRRDHLERFVPRLPDSHFDLSTIVDARDFEHLAEIREGTREDAIEGLMLKRRDSPYIAGRKTGLWYKWKR
DPLLVDCVLMYAQRGSGKRSSFYSDYTFGCWDGDPDKGAELLPVGKAYSGFTDEELKKLDRLVRQNTLNRFGPVREVERT
LVFEVAFDSVHTSKRHKSGLAMRFPRIHRIRWDKPVHEADRLQTLKAMIQD
>Mature_531_residues
MQAFADLIDALVYTRSRNEKLRLIAAYLRETPDPDRGWALAALTDGLDFPAVKSSAIRTIMKERIDPVLWTLSRDFVGDT
AETASLLWPAPDSDVTPPPSVSEVVDLLSKMTRQSVLTELPALLDRLDPSGRYALLKIATGAMRIGVSSRLAKVAFAQAF
GVDVEDVEEYWHGLEPPYEELFAWAADGADPPDTENKPTFRPFMLAHPLEDTVVDMADYAAEWKWDGIRVQLVHAGGETR
IYSRSGDDISRTFPEMVDALQVPAVLDGELLVRGDAQGGEAGGAASFNALQQRLGRKTVSKKMLSESPAFVRLYDALIIE
GEDLRDQPWERRRDHLERFVPRLPDSHFDLSTIVDARDFEHLAEIREGTREDAIEGLMLKRRDSPYIAGRKTGLWYKWKR
DPLLVDCVLMYAQRGSGKRSSFYSDYTFGCWDGDPDKGAELLPVGKAYSGFTDEELKKLDRLVRQNTLNRFGPVREVERT
LVFEVAFDSVHTSKRHKSGLAMRFPRIHRIRWDKPVHEADRLQTLKAMIQD

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=355, Percent_Identity=29.0140845070423, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71985269, Length=496, Percent_Identity=25, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320038, Length=357, Percent_Identity=28.8515406162465, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24762424, Length=344, Percent_Identity=25.8720930232558, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI85815841, Length=345, Percent_Identity=26.0869565217391, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 59994; Mature: 59994

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFADLIDALVYTRSRNEKLRLIAAYLRETPDPDRGWALAALTDGLDFPAVKSSAIRTI
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
MKERIDPVLWTLSRDFVGDTAETASLLWPAPDSDVTPPPSVSEVVDLLSKMTRQSVLTEL
HHHHCCHHHHHHHHHHCCCCHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
PALLDRLDPSGRYALLKIATGAMRIGVSSRLAKVAFAQAFGVDVEDVEEYWHGLEPPYEE
HHHHHHCCCCCCEEEEEEECCHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHH
LFAWAADGADPPDTENKPTFRPFMLAHPLEDTVVDMADYAAEWKWDGIRVQLVHAGGETR
HHHHHCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCEECCEEEEEEECCCCEE
IYSRSGDDISRTFPEMVDALQVPAVLDGELLVRGDAQGGEAGGAASFNALQQRLGRKTVS
EEECCCCHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
KKMLSESPAFVRLYDALIIEGEDLRDQPWERRRDHLERFVPRLPDSHFDLSTIVDARDFE
HHHHHCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
HLAEIREGTREDAIEGLMLKRRDSPYIAGRKTGLWYKWKRDPLLVDCVLMYAQRGSGKRS
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCC
SFYSDYTFGCWDGDPDKGAELLPVGKAYSGFTDEELKKLDRLVRQNTLNRFGPVREVERT
CCCCCCEEECCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LVFEVAFDSVHTSKRHKSGLAMRFPRIHRIRWDKPVHEADRLQTLKAMIQD
HHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQAFADLIDALVYTRSRNEKLRLIAAYLRETPDPDRGWALAALTDGLDFPAVKSSAIRTI
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
MKERIDPVLWTLSRDFVGDTAETASLLWPAPDSDVTPPPSVSEVVDLLSKMTRQSVLTEL
HHHHCCHHHHHHHHHHCCCCHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
PALLDRLDPSGRYALLKIATGAMRIGVSSRLAKVAFAQAFGVDVEDVEEYWHGLEPPYEE
HHHHHHCCCCCCEEEEEEECCHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHH
LFAWAADGADPPDTENKPTFRPFMLAHPLEDTVVDMADYAAEWKWDGIRVQLVHAGGETR
HHHHHCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCEECCEEEEEEECCCCEE
IYSRSGDDISRTFPEMVDALQVPAVLDGELLVRGDAQGGEAGGAASFNALQQRLGRKTVS
EEECCCCHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
KKMLSESPAFVRLYDALIIEGEDLRDQPWERRRDHLERFVPRLPDSHFDLSTIVDARDFE
HHHHHCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
HLAEIREGTREDAIEGLMLKRRDSPYIAGRKTGLWYKWKRDPLLVDCVLMYAQRGSGKRS
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCC
SFYSDYTFGCWDGDPDKGAELLPVGKAYSGFTDEELKKLDRLVRQNTLNRFGPVREVERT
CCCCCCEEECCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LVFEVAFDSVHTSKRHKSGLAMRFPRIHRIRWDKPVHEADRLQTLKAMIQD
HHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA