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The map label for this gene is hemB [H]

Identifier: 85375456

GI number: 85375456

Start: 2647069

End: 2648001

Strand: Direct

Name: hemB [H]

Synonym: ELI_13145

Alternate gene names: 85375456

Gene position: 2647069-2648001 (Clockwise)

Preceding gene: 85375455

Following gene: 85375457

Centisome position: 86.72

GC content: 61.74

Gene sequence:

>933_bases
ATGTTTCGCGAAACCGTGCTTACGCCAGCCGACCTGATCTGGCCATTGTTCATCATCGAGGGTGAGGGACAGGAGGAGCC
GGTCGAGAGCCTCCCCGGCGTTTCGCGCTGGTCGGTCGACGGGATTGTCGCGCGGGCCAAAGAGGCGATCGACCTGGGCA
TCCCGTGCATCGCGCTGTTTCCCAACACGCCGAGCAAGTTGCGCAGCGACGATGGCAGGGAAGCGTACAATCCCGACAAT
CTGATGTGCCGCGCGATCAAGGCGGTACGCGATGCTTGCGGCAGCGATATCGGCATCCTCACCGATGTCGCGCTCGATCC
CTATACCAGCCACGGGCAAGACGGCTTGCTCGACAATGCGGGCTATGTCGTCAATGACGATACCGTTGCCGTGCTGGTCG
ACCAGGCCGTCAATCAGGCCGAAGCGGGGGCCGATATCATCGCTCCGTCGGACATGATGGATGGACGGGTCAAGGCCATC
CGCATGGCGCTGGAGATGGGCGAGCATCCCAATGTCCAGATCATGGCCTATGCCGCGAAATATGCCTCAGCCTTTTACGG
ACCGTTTCGCGATGCGGTCAGCAGCGGCGGGCTGCTCAAAGGCGACAAGAAAAGCTACCAGATGGATCCGGCCAATGCGC
TCGAGGCACTGCGCGAGATCGAGCTGGACATCGCCGAGGGCGCCGATAGCGTGATGGTCAAGCCCGGCCTCGCCTATCTC
GATATCATCTGGCGCGCGAAGGAAGCTTTCGGCGTGCCGGTGTTCGCTTACCAGGTCAGCGGCGAATACGCGCTGATAGA
AGCGGGTGCTGCGGCAGGCGTCGGCGATCGCGACGCGCTGATGATGGAAAAACTTATTGCCTTCAAGCGGGCCGGTTGTG
CGGGGGTCCTGACGTATCACGCACCGGTCGCTGCCAAAATGCTCGATGGCTGA

Upstream 100 bases:

>100_bases
ATTGCGCTGGCATAGTGAATGCGGCAACACTTGCCGCATGTCAGCATCCTATCCTGCAACGCGCCTGCGCCGCACCCGGG
CCACCGCCTGGAGTCGCGCG

Downstream 100 bases:

>100_bases
ATTTCGGCACGAGACCGGGCGACTGGTGCTGCGCGATTGGCGCGACGCGGATTGGCCGGAGTTCTGGGAAGGCACCAATA
CACCGGCTGTGATGCGCTGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MFRETVLTPADLIWPLFIIEGEGQEEPVESLPGVSRWSVDGIVARAKEAIDLGIPCIALFPNTPSKLRSDDGREAYNPDN
LMCRAIKAVRDACGSDIGILTDVALDPYTSHGQDGLLDNAGYVVNDDTVAVLVDQAVNQAEAGADIIAPSDMMDGRVKAI
RMALEMGEHPNVQIMAYAAKYASAFYGPFRDAVSSGGLLKGDKKSYQMDPANALEALREIELDIAEGADSVMVKPGLAYL
DIIWRAKEAFGVPVFAYQVSGEYALIEAGAAAGVGDRDALMMEKLIAFKRAGCAGVLTYHAPVAAKMLDG

Sequences:

>Translated_310_residues
MFRETVLTPADLIWPLFIIEGEGQEEPVESLPGVSRWSVDGIVARAKEAIDLGIPCIALFPNTPSKLRSDDGREAYNPDN
LMCRAIKAVRDACGSDIGILTDVALDPYTSHGQDGLLDNAGYVVNDDTVAVLVDQAVNQAEAGADIIAPSDMMDGRVKAI
RMALEMGEHPNVQIMAYAAKYASAFYGPFRDAVSSGGLLKGDKKSYQMDPANALEALREIELDIAEGADSVMVKPGLAYL
DIIWRAKEAFGVPVFAYQVSGEYALIEAGAAAGVGDRDALMMEKLIAFKRAGCAGVLTYHAPVAAKMLDG
>Mature_310_residues
MFRETVLTPADLIWPLFIIEGEGQEEPVESLPGVSRWSVDGIVARAKEAIDLGIPCIALFPNTPSKLRSDDGREAYNPDN
LMCRAIKAVRDACGSDIGILTDVALDPYTSHGQDGLLDNAGYVVNDDTVAVLVDQAVNQAEAGADIIAPSDMMDGRVKAI
RMALEMGEHPNVQIMAYAAKYASAFYGPFRDAVSSGGLLKGDKKSYQMDPANALEALREIELDIAEGADSVMVKPGLAYL
DIIWRAKEAFGVPVFAYQVSGEYALIEAGAAAGVGDRDALMMEKLIAFKRAGCAGVLTYHAPVAAKMLDG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=301, Percent_Identity=39.5348837209302, Blast_Score=209, Evalue=3e-54,
Organism=Escherichia coli, GI87081728, Length=301, Percent_Identity=42.8571428571429, Blast_Score=206, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321398, Length=310, Percent_Identity=38.0645161290323, Blast_Score=179, Evalue=6e-46,
Organism=Drosophila melanogaster, GI21358291, Length=297, Percent_Identity=36.7003367003367, Blast_Score=201, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 33103; Mature: 33103

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRETVLTPADLIWPLFIIEGEGQEEPVESLPGVSRWSVDGIVARAKEAIDLGIPCIALF
CCCCCCCCHHHHEEEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
PNTPSKLRSDDGREAYNPDNLMCRAIKAVRDACGSDIGILTDVALDPYTSHGQDGLLDNA
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCC
GYVVNDDTVAVLVDQAVNQAEAGADIIAPSDMMDGRVKAIRMALEMGEHPNVQIMAYAAK
CEEECCCEEEEEEHHHHHHHHCCCCEECCCHHCCHHHHHHHHHHHCCCCCCEEEEEEHHH
YASAFYGPFRDAVSSGGLLKGDKKSYQMDPANALEALREIELDIAEGADSVMVKPGLAYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHH
DIIWRAKEAFGVPVFAYQVSGEYALIEAGAAAGVGDRDALMMEKLIAFKRAGCAGVLTYH
HHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
APVAAKMLDG
CCHHHHHCCC
>Mature Secondary Structure
MFRETVLTPADLIWPLFIIEGEGQEEPVESLPGVSRWSVDGIVARAKEAIDLGIPCIALF
CCCCCCCCHHHHEEEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
PNTPSKLRSDDGREAYNPDNLMCRAIKAVRDACGSDIGILTDVALDPYTSHGQDGLLDNA
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCC
GYVVNDDTVAVLVDQAVNQAEAGADIIAPSDMMDGRVKAIRMALEMGEHPNVQIMAYAAK
CEEECCCEEEEEEHHHHHHHHCCCCEECCCHHCCHHHHHHHHHHHCCCCCCEEEEEEHHH
YASAFYGPFRDAVSSGGLLKGDKKSYQMDPANALEALREIELDIAEGADSVMVKPGLAYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHH
DIIWRAKEAFGVPVFAYQVSGEYALIEAGAAAGVGDRDALMMEKLIAFKRAGCAGVLTYH
HHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
APVAAKMLDG
CCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]