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The map label for this gene is ptsN [H]

Identifier: 85375421

GI number: 85375421

Start: 2613283

End: 2613744

Strand: Reverse

Name: ptsN [H]

Synonym: ELI_12970

Alternate gene names: 85375421

Gene position: 2613744-2613283 (Counterclockwise)

Preceding gene: 85375422

Following gene: 85375420

Centisome position: 85.63

GC content: 66.45

Gene sequence:

>462_bases
ATGAATGCGCTTTTCTCTTTCCCGCCCGAAGCCGTCGCCAGCGCATCTGCCGACACCAAGGGCGAGGTCCTGTCGCTGCT
GGCCGATCATTTCGCGCGGATCTACGATCTCGACGCTGGCGGCGTGCTCGATGCGCTGGAAGAGCGCGAGAAACTGGGCA
GTACGGGTTTCGGGCGCGGCGTGGCGATTCCGCATGCCCGCCTCGATACGATCTCGCGCCCGCTGGCGGTCCTGCTGCGG
CTCGACGCGGCTTGCGATTTCGCGGCCGCCGACGGCATGCCGGTCGACCTGGTCTTCGGCTTGCTGTCGCCCAGCAATGC
AGGAGCAACGCATTTGCACGCGCTGGCGGCGATTTCACGGCTGGTGCGCGACGAGGAGTCGCATCGCCTGCTGACCGAAG
CGCCCGATGCCGAAGCGCTCTACGCGCTGATTACCAACGCGGCGGATCGTGACGCAGCCTGA

Upstream 100 bases:

>100_bases
CACATCGGTATCGAGCAGCGCCTGATCGCGGGCGTTCGTCGCTTGTCTGTCCGCGCGGTCCGAAACCTGTCGAGTGCTGG
AAATACCCCCGAGTTTCACC

Downstream 100 bases:

>100_bases
GCAGACCCCCGGGGCGGACCTGCATTACCGCGCGCTCGAGCGTCTTTACACCGCCGCTCCCGTCAACCATCTGTTCGAAT
CCGCGCTGGCGATCGAAGAG

Product: nitrogen regulatory IIA

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MNALFSFPPEAVASASADTKGEVLSLLADHFARIYDLDAGGVLDALEEREKLGSTGFGRGVAIPHARLDTISRPLAVLLR
LDAACDFAAADGMPVDLVFGLLSPSNAGATHLHALAAISRLVRDEESHRLLTEAPDAEALYALITNAADRDAA

Sequences:

>Translated_153_residues
MNALFSFPPEAVASASADTKGEVLSLLADHFARIYDLDAGGVLDALEEREKLGSTGFGRGVAIPHARLDTISRPLAVLLR
LDAACDFAAADGMPVDLVFGLLSPSNAGATHLHALAAISRLVRDEESHRLLTEAPDAEALYALITNAADRDAA
>Mature_153_residues
MNALFSFPPEAVASASADTKGEVLSLLADHFARIYDLDAGGVLDALEEREKLGSTGFGRGVAIPHARLDTISRPLAVLLR
LDAACDFAAADGMPVDLVFGLLSPSNAGATHLHALAAISRLVRDEESHRLLTEAPDAEALYALITNAADRDAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=133, Percent_Identity=37.593984962406, Blast_Score=90, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 16034; Mature: 16034

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALFSFPPEAVASASADTKGEVLSLLADHFARIYDLDAGGVLDALEEREKLGSTGFGRG
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCC
VAIPHARLDTISRPLAVLLRLDAACDFAAADGMPVDLVFGLLSPSNAGATHLHALAAISR
CCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
LVRDEESHRLLTEAPDAEALYALITNAADRDAA
HHHCCHHCCHHHCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNALFSFPPEAVASASADTKGEVLSLLADHFARIYDLDAGGVLDALEEREKLGSTGFGRG
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCC
VAIPHARLDTISRPLAVLLRLDAACDFAAADGMPVDLVFGLLSPSNAGATHLHALAAISR
CCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
LVRDEESHRLLTEAPDAEALYALITNAADRDAA
HHHCCHHCCHHHCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]