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The map label for this gene is purD [H]

Identifier: 85375370

GI number: 85375370

Start: 2570695

End: 2571975

Strand: Direct

Name: purD [H]

Synonym: ELI_12715

Alternate gene names: 85375370

Gene position: 2570695-2571975 (Clockwise)

Preceding gene: 85375365

Following gene: 85375372

Centisome position: 84.22

GC content: 64.64

Gene sequence:

>1281_bases
GTGAACATTCTCCTCATCGGCTCCGGAGGGCGCGAACACGCGCTGGCATGGAAATTGGCGCAGTCACCCAGCTGCACAAA
GCTCTATGCAGCGCCGGGCAATCCGGGGATTGCCGAGGATGCCGAATGCGTGACGCTGGATGTGGCGGATCATGGCGCAG
TGGCAGAATTCTGCACCAATAAGGACATTGCACTGGTCGTCGTGGGACCCGAAGCGCCGCTGGTCGATGGGTTGGGCGAC
AGTTTGCGCGCGGCGGGCATCGCGGTGTTCGGTCCGAACGCCAAGCCCGCCCAGCTGGAGGGCTCGAAGGGGTTCACGAA
GGACCTGTGCGAACGCGCTGGAATTCCGACGGCGGGCTATGTCCGCACGACTTCGCTTGATGAAGCGACGCAGGCACTGG
AGCGCTTCGAGGCACCCCACGTTCTAAAGGCCGACGGACTGGCGGCAGGCAAGGGCGTGGTCATCGCCGAGACGCGCGAA
GATGCCCAGGCCGCGCTCGCCGACATGTTCGGCGGACAGTTCGGCGAAGCGGGTGCGGAAGTCGTGATCGAGGAGTTCAT
GGCGGGCGAGGAAGCGAGCTTCTTCGCGCTGACCGACGGAACGGCAATCGTGCCCATCGGCACTGCGCAGGACCACAAGC
GCATCGGCGACGGCGATGTGGGACCCAATACAGGCGGTATGGGGGCCTATTCGCCCGCACCGATCCTCACCGACGATTTA
CAGGCGCGCGTGATGGCCGAGATCATCGAGCCCACCGTCACGACCCTACGCGAAGAGGGCATGCCCTATTCCGGCGTACT
CTATGCCGGGCTGATGCTGACCGAGACCGGCCCAAAACTGATCGAATACAACGCCCGGTTCGGTGATCCCGAATGCCAGG
TATTGATGATGCGGCTGCAGGAGGACCTCGCAGAGCTGATGCTGGCCTGCGCCGAGGGGCGCTTAGGCGAGTTCTCTGAT
GCGAAGATGGCTCCCGAAACCGCGCTCACGGTCGTGATGGCGGCCAAGGGCTATCCCGGCACACCCGAAAAGGGCGGCAG
CATCGACCTTGGCCAGGCCCAGGCAAATGGCGCGAAAGTCTTCCACGCTGGCACCAAACGGGACGCTAACGGCACGCTGG
TCGCCAACGGCGGCCGTGTCCTCAACGTCACTGCCACAGGCCCGAACGTGTCCGAAGCCCAGCGCGCCGCCTACCAGGCT
GTCGACGCGATCGACTTCCCCACCGGCTTCTGCCGCCGCGATATCGGCTGGCGCGAGATCAAGCGGGAGCGGAAAGCCTA
G

Upstream 100 bases:

>100_bases
TTAGGAGGTGGCTTCTCTACCCGTTCGTGCTGAGCTTGTCGGTAGCGAAGCTGGCGCGTACCGCCAACCACTGTCCTTAC
TTTTGCGCGAGGTTTGCCAC

Downstream 100 bases:

>100_bases
CTCAGCTTCTGAGCCATCAGCGCTTTCATATCGACCTCGGGGCGCGGGCCGTAGTGGCTGATCACTTCCGCCGCTGCGAT
TGCGCCGCGCTTGAGGCAGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNILLIGSGGREHALAWKLAQSPSCTKLYAAPGNPGIAEDAECVTLDVADHGAVAEFCTNKDIALVVVGPEAPLVDGLGD
SLRAAGIAVFGPNAKPAQLEGSKGFTKDLCERAGIPTAGYVRTTSLDEATQALERFEAPHVLKADGLAAGKGVVIAETRE
DAQAALADMFGGQFGEAGAEVVIEEFMAGEEASFFALTDGTAIVPIGTAQDHKRIGDGDVGPNTGGMGAYSPAPILTDDL
QARVMAEIIEPTVTTLREEGMPYSGVLYAGLMLTETGPKLIEYNARFGDPECQVLMMRLQEDLAELMLACAEGRLGEFSD
AKMAPETALTVVMAAKGYPGTPEKGGSIDLGQAQANGAKVFHAGTKRDANGTLVANGGRVLNVTATGPNVSEAQRAAYQA
VDAIDFPTGFCRRDIGWREIKRERKA

Sequences:

>Translated_426_residues
MNILLIGSGGREHALAWKLAQSPSCTKLYAAPGNPGIAEDAECVTLDVADHGAVAEFCTNKDIALVVVGPEAPLVDGLGD
SLRAAGIAVFGPNAKPAQLEGSKGFTKDLCERAGIPTAGYVRTTSLDEATQALERFEAPHVLKADGLAAGKGVVIAETRE
DAQAALADMFGGQFGEAGAEVVIEEFMAGEEASFFALTDGTAIVPIGTAQDHKRIGDGDVGPNTGGMGAYSPAPILTDDL
QARVMAEIIEPTVTTLREEGMPYSGVLYAGLMLTETGPKLIEYNARFGDPECQVLMMRLQEDLAELMLACAEGRLGEFSD
AKMAPETALTVVMAAKGYPGTPEKGGSIDLGQAQANGAKVFHAGTKRDANGTLVANGGRVLNVTATGPNVSEAQRAAYQA
VDAIDFPTGFCRRDIGWREIKRERKA
>Mature_426_residues
MNILLIGSGGREHALAWKLAQSPSCTKLYAAPGNPGIAEDAECVTLDVADHGAVAEFCTNKDIALVVVGPEAPLVDGLGD
SLRAAGIAVFGPNAKPAQLEGSKGFTKDLCERAGIPTAGYVRTTSLDEATQALERFEAPHVLKADGLAAGKGVVIAETRE
DAQAALADMFGGQFGEAGAEVVIEEFMAGEEASFFALTDGTAIVPIGTAQDHKRIGDGDVGPNTGGMGAYSPAPILTDDL
QARVMAEIIEPTVTTLREEGMPYSGVLYAGLMLTETGPKLIEYNARFGDPECQVLMMRLQEDLAELMLACAEGRLGEFSD
AKMAPETALTVVMAAKGYPGTPEKGGSIDLGQAQANGAKVFHAGTKRDANGTLVANGGRVLNVTATGPNVSEAQRAAYQA
VDAIDFPTGFCRRDIGWREIKRERKA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=48.1132075471698, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=48.2269503546099, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=48.2269503546099, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=48.2269503546099, Blast_Score=381, Evalue=1e-106,
Organism=Escherichia coli, GI1790438, Length=430, Percent_Identity=53.2558139534884, Blast_Score=430, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=40.9952606635071, Blast_Score=293, Evalue=9e-80,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=42.6966292134831, Blast_Score=336, Evalue=4e-93,
Organism=Drosophila melanogaster, GI62471619, Length=413, Percent_Identity=44.5520581113801, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24582400, Length=413, Percent_Identity=44.5520581113801, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44486; Mature: 44486

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLIGSGGREHALAWKLAQSPSCTKLYAAPGNPGIAEDAECVTLDVADHGAVAEFCTN
CEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHCC
KDIALVVVGPEAPLVDGLGDSLRAAGIAVFGPNAKPAQLEGSKGFTKDLCERAGIPTAGY
CCEEEEEECCCCCCCCCCCCHHHHCEEEEECCCCCCCEECCCCCCHHHHHHHCCCCCCCE
VRTTSLDEATQALERFEAPHVLKADGLAAGKGVVIAETREDAQAALADMFGGQFGEAGAE
EEECCHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHH
VVIEEFMAGEEASFFALTDGTAIVPIGTAQDHKRIGDGDVGPNTGGMGAYSPAPILTDDL
HHHHHHHCCCCCCEEEEECCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
QARVMAEIIEPTVTTLREEGMPYSGVLYAGLMLTETGPKLIEYNARFGDPECQVLMMRLQ
HHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCCCEEEECCCCCCHHHHHHHHHHH
EDLAELMLACAEGRLGEFSDAKMAPETALTVVMAAKGYPGTPEKGGSIDLGQAQANGAKV
HHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCEECCCCCCCCCEE
FHAGTKRDANGTLVANGGRVLNVTATGPNVSEAQRAAYQAVDAIDFPTGFCRRDIGWREI
EECCCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHH
KRERKA
HHHHCC
>Mature Secondary Structure
MNILLIGSGGREHALAWKLAQSPSCTKLYAAPGNPGIAEDAECVTLDVADHGAVAEFCTN
CEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHCC
KDIALVVVGPEAPLVDGLGDSLRAAGIAVFGPNAKPAQLEGSKGFTKDLCERAGIPTAGY
CCEEEEEECCCCCCCCCCCCHHHHCEEEEECCCCCCCEECCCCCCHHHHHHHCCCCCCCE
VRTTSLDEATQALERFEAPHVLKADGLAAGKGVVIAETREDAQAALADMFGGQFGEAGAE
EEECCHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHH
VVIEEFMAGEEASFFALTDGTAIVPIGTAQDHKRIGDGDVGPNTGGMGAYSPAPILTDDL
HHHHHHHCCCCCCEEEEECCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
QARVMAEIIEPTVTTLREEGMPYSGVLYAGLMLTETGPKLIEYNARFGDPECQVLMMRLQ
HHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCCCEEEECCCCCCHHHHHHHHHHH
EDLAELMLACAEGRLGEFSDAKMAPETALTVVMAAKGYPGTPEKGGSIDLGQAQANGAKV
HHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCEECCCCCCCCCEE
FHAGTKRDANGTLVANGGRVLNVTATGPNVSEAQRAAYQAVDAIDFPTGFCRRDIGWREI
EECCCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHH
KRERKA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]