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The map label for this gene is purH

Identifier: 85375335

GI number: 85375335

Start: 2536292

End: 2537878

Strand: Reverse

Name: purH

Synonym: ELI_12540

Alternate gene names: 85375335

Gene position: 2537878-2536292 (Counterclockwise)

Preceding gene: 85375336

Following gene: 85375334

Centisome position: 83.14

GC content: 64.27

Gene sequence:

>1587_bases
GTGACTGATGTCGTCATCAAGCGGGCGCTGCTTTCGGTGTCCGACAAGACCGGCTTGGTGGAACTGGGGCAGGCGCTGGC
CGACCATGGTGTCGAACTGGTCTCGACCGGCGGCACGGCGAAGGCGCTGCGCGAGGCCGGGCTGCAAGTGAAAGACATCT
CCGACCTCACCGGCTTCCCGGAAATGATGGATGGCCGCGTCAAGACGCTTCATCCGAAGGTCCATGGCGGTTTGCTGGCG
GTACGCGACAATGCCGAGCATGCGGCGGCGATGGAAGAGCACGATATTGGCGCGATCGACCTAGTCGTCGTCAATCTCTA
CCCGTTCGAAGCAACCGTGATGCGCGGCGCCGAGCGTGACGAGATTATCGAGAATATCGACATCGGCGGGCCGAGCATGG
TCCGCAGCTCGGCCAAGAACCACGCCTATGTCACCATCGTCACCGATCCCGCGGACTACGAAACCCTGCTGGGCGAGATG
AAAGATGGCGGGGCGACGTCGCTCGATTTTCGCAAGGCGATGGCAGCCAAGGCCTTTGCCGCGACCGCCAGCTACGATTC
GATGATCAGCCAGTGGTTTGCTTTTGCCGACCAGGGACAGTTGTTCCCCGACATGCTGGCGATCAACGGCAAGGCACCGA
CGCCCCTGCGCTATGGCGAGAACCCGCACCAGATGGCGGCGCTTTATACGCCTGTCGGCCCGCATACGCAGGGGATAGCG
CAGGCGAAACAGGTGCAGGGCAAGGAACTCAGCTACAACAATTACAACGACGCCGACGCCGCGCTGGAGCTGTGCGCCGA
GTTCAAGGAAGGCGATCCGGCGGTTGTCATCGTCAAGCATGCCAATCCGTGCGGCGTGGCGCAGGCGGGCTCGCTGCTGG
AGGCGTGGGAACAAGCGCTCGCCTGCGACAGCGTGTCGGCTTTCGGCGGGATCGTCGCGGTCAACCGGACGCTCGACGGG
CCGACGGCGGAAGCTATCGCCAAGATCTTCACCGAAGTCGTGGTCGCGCCCGATGCCGACGAGGCGGCGCGTGAAGCCTT
TGCCAAGAAGAAGAACCTGCGGCTCCTCCTGACCGGCGACCTGCCCGACCCGCGCCGCGGCGGGCTGGCGGTGAAGCCGA
TCACAGGCGGATTGCTGGTGCAGTCGCGCGACAACGGTGCGCTGACCGTTGATGCGCTCAAGGTCGTGACCGAACGCGAG
CCTTCCGAGCAGGAACTCAAGGACTGCCTGTTCGCGTGGACCGTCGCGCGTCACGTCAAATCCAACGCCATCGTCTATGC
CAAGGACGGCGCGACTGCGGGCATCGGTGCGGGCCAGATGAATCGGCGCGATTCATCGCGCATCGCGGCAATGAAAGCTG
CCGAAGCGGCGGAGAAATACGATTGGCCGCAGCCGCGCACCGTCGGCAGCGCCGTCGCGTCGGATGCGTTTTTTCCCTTC
GCCGACGGGCTGCTGGCAGCCGCCGAAGCCGGGGCGACCGCGGTGATCCAACCGGGCGGCTCGATCCGCGATGACGAAGT
GATCGAGGCGGCCAACAAGGCGGGGCTGGCGATGGTTTTCACCGGAATGCGGCATTTCAGACATTAA

Upstream 100 bases:

>100_bases
AATGGCCGCCCGCAGCCGATCGAGCAATTGGTGGTCCAGGGTATGACCAGCCGCAGCGGGGGCTCGTTCTCCTGGCTGCT
CAAACAGATGGGGTAATTAC

Downstream 100 bases:

>100_bases
TCACGCAGCCGTTGCTGTCGTATCTTCCGCGAAGGCGGGGACCTCGGTCGGTTTTGCGCCACCCCTGCCAACCGCGACTT
TCGTACCGATTGTAACCGAT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MTDVVIKRALLSVSDKTGLVELGQALADHGVELVSTGGTAKALREAGLQVKDISDLTGFPEMMDGRVKTLHPKVHGGLLA
VRDNAEHAAAMEEHDIGAIDLVVVNLYPFEATVMRGAERDEIIENIDIGGPSMVRSSAKNHAYVTIVTDPADYETLLGEM
KDGGATSLDFRKAMAAKAFAATASYDSMISQWFAFADQGQLFPDMLAINGKAPTPLRYGENPHQMAALYTPVGPHTQGIA
QAKQVQGKELSYNNYNDADAALELCAEFKEGDPAVVIVKHANPCGVAQAGSLLEAWEQALACDSVSAFGGIVAVNRTLDG
PTAEAIAKIFTEVVVAPDADEAAREAFAKKKNLRLLLTGDLPDPRRGGLAVKPITGGLLVQSRDNGALTVDALKVVTERE
PSEQELKDCLFAWTVARHVKSNAIVYAKDGATAGIGAGQMNRRDSSRIAAMKAAEAAEKYDWPQPRTVGSAVASDAFFPF
ADGLLAAAEAGATAVIQPGGSIRDDEVIEAANKAGLAMVFTGMRHFRH

Sequences:

>Translated_528_residues
MTDVVIKRALLSVSDKTGLVELGQALADHGVELVSTGGTAKALREAGLQVKDISDLTGFPEMMDGRVKTLHPKVHGGLLA
VRDNAEHAAAMEEHDIGAIDLVVVNLYPFEATVMRGAERDEIIENIDIGGPSMVRSSAKNHAYVTIVTDPADYETLLGEM
KDGGATSLDFRKAMAAKAFAATASYDSMISQWFAFADQGQLFPDMLAINGKAPTPLRYGENPHQMAALYTPVGPHTQGIA
QAKQVQGKELSYNNYNDADAALELCAEFKEGDPAVVIVKHANPCGVAQAGSLLEAWEQALACDSVSAFGGIVAVNRTLDG
PTAEAIAKIFTEVVVAPDADEAAREAFAKKKNLRLLLTGDLPDPRRGGLAVKPITGGLLVQSRDNGALTVDALKVVTERE
PSEQELKDCLFAWTVARHVKSNAIVYAKDGATAGIGAGQMNRRDSSRIAAMKAAEAAEKYDWPQPRTVGSAVASDAFFPF
ADGLLAAAEAGATAVIQPGGSIRDDEVIEAANKAGLAMVFTGMRHFRH
>Mature_527_residues
TDVVIKRALLSVSDKTGLVELGQALADHGVELVSTGGTAKALREAGLQVKDISDLTGFPEMMDGRVKTLHPKVHGGLLAV
RDNAEHAAAMEEHDIGAIDLVVVNLYPFEATVMRGAERDEIIENIDIGGPSMVRSSAKNHAYVTIVTDPADYETLLGEMK
DGGATSLDFRKAMAAKAFAATASYDSMISQWFAFADQGQLFPDMLAINGKAPTPLRYGENPHQMAALYTPVGPHTQGIAQ
AKQVQGKELSYNNYNDADAALELCAEFKEGDPAVVIVKHANPCGVAQAGSLLEAWEQALACDSVSAFGGIVAVNRTLDGP
TAEAIAKIFTEVVVAPDADEAAREAFAKKKNLRLLLTGDLPDPRRGGLAVKPITGGLLVQSRDNGALTVDALKVVTEREP
SEQELKDCLFAWTVARHVKSNAIVYAKDGATAGIGAGQMNRRDSSRIAAMKAAEAAEKYDWPQPRTVGSAVASDAFFPFA
DGLLAAAEAGATAVIQPGGSIRDDEVIEAANKAGLAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=605, Percent_Identity=36.0330578512397, Blast_Score=299, Evalue=4e-81,
Organism=Escherichia coli, GI1790439, Length=535, Percent_Identity=55.7009345794393, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71985564, Length=606, Percent_Identity=33.3333333333333, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI71985574, Length=331, Percent_Identity=27.7945619335347, Blast_Score=92, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323056, Length=605, Percent_Identity=33.2231404958678, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6323768, Length=606, Percent_Identity=32.1782178217822, Blast_Score=274, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24649832, Length=603, Percent_Identity=34.3283582089552, Blast_Score=289, Evalue=3e-78,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55920; Mature: 55789

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVVIKRALLSVSDKTGLVELGQALADHGVELVSTGGTAKALREAGLQVKDISDLTGFP
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCCEEEECHHHCCCH
EMMDGRVKTLHPKVHGGLLAVRDNAEHAAAMEEHDIGAIDLVVVNLYPFEATVMRGAERD
HHHCCCEEEECCHHCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEEECCHHHHHCCCCHH
EIIENIDIGGPSMVRSSAKNHAYVTIVTDPADYETLLGEMKDGGATSLDFRKAMAAKAFA
HHHHHCCCCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ATASYDSMISQWFAFADQGQLFPDMLAINGKAPTPLRYGENPHQMAALYTPVGPHTQGIA
HHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCCCCHHHEEEEECCCCCCHHHHH
QAKQVQGKELSYNNYNDADAALELCAEFKEGDPAVVIVKHANPCGVAQAGSLLEAWEQAL
HHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
ACDSVSAFGGIVAVNRTLDGPTAEAIAKIFTEVVVAPDADEAAREAFAKKKNLRLLLTGD
HHCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCEEEEECC
LPDPRRGGLAVKPITGGLLVQSRDNGALTVDALKVVTEREPSEQELKDCLFAWTVARHVK
CCCCCCCCEEEEECCCCEEEEECCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
SNAIVYAKDGATAGIGAGQMNRRDSSRIAAMKAAEAAEKYDWPQPRTVGSAVASDAFFPF
CCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHH
ADGLLAAAEAGATAVIQPGGSIRDDEVIEAANKAGLAMVFTGMRHFRH
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TDVVIKRALLSVSDKTGLVELGQALADHGVELVSTGGTAKALREAGLQVKDISDLTGFP
CHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCCEEEECHHHCCCH
EMMDGRVKTLHPKVHGGLLAVRDNAEHAAAMEEHDIGAIDLVVVNLYPFEATVMRGAERD
HHHCCCEEEECCHHCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEEECCHHHHHCCCCHH
EIIENIDIGGPSMVRSSAKNHAYVTIVTDPADYETLLGEMKDGGATSLDFRKAMAAKAFA
HHHHHCCCCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ATASYDSMISQWFAFADQGQLFPDMLAINGKAPTPLRYGENPHQMAALYTPVGPHTQGIA
HHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCCCCHHHEEEEECCCCCCHHHHH
QAKQVQGKELSYNNYNDADAALELCAEFKEGDPAVVIVKHANPCGVAQAGSLLEAWEQAL
HHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
ACDSVSAFGGIVAVNRTLDGPTAEAIAKIFTEVVVAPDADEAAREAFAKKKNLRLLLTGD
HHCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCEEEEECC
LPDPRRGGLAVKPITGGLLVQSRDNGALTVDALKVVTEREPSEQELKDCLFAWTVARHVK
CCCCCCCCEEEEECCCCEEEEECCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
SNAIVYAKDGATAGIGAGQMNRRDSSRIAAMKAAEAAEKYDWPQPRTVGSAVASDAFFPF
CCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHH
ADGLLAAAEAGATAVIQPGGSIRDDEVIEAANKAGLAMVFTGMRHFRH
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA