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The map label for this gene is thrS

Identifier: 85375301

GI number: 85375301

Start: 2499817

End: 2501835

Strand: Reverse

Name: thrS

Synonym: ELI_12370

Alternate gene names: 85375301

Gene position: 2501835-2499817 (Counterclockwise)

Preceding gene: 85375302

Following gene: 85375300

Centisome position: 81.96

GC content: 64.54

Gene sequence:

>2019_bases
ATGACGGAACTACTGAAAATCAGCCTGCCCGATGGCTCGGTGCGCGAAATGGAAGCGGGCGCCACGCCCGCCGATGTCGC
GGCGGCGATCGGCCCGGGCCTCGCCAAGGCTGCGCTGGCGGCGAAAGTCGACGGCGAAGTCCGCGATCTCAACCGCCCCT
TCGAAGGCGATGCCGAACTCGCGCTGATCACGTCGCGCGACGAGGAAGAAGCGCTCGAGCTGGCGCGGCACGACTACGCG
CATGTCCTGGCGGAAGCGGTGCAGGCGCTGTGGCCCGGCACACAGATCACCTTCGGCCCGGCGACCGACGACGGTTTCTA
TTACGATGTCAAAGCGCCTGACAGTCGCGATCCCTTCGGCATGGACGATCTGCCTGCCATCGAAGAAAAGATGCGCGAGA
TCATCAAGGCCGACAAGCCGCTGGTGCGCGAGGTCTGGAGCCGCGAGCAGCTGATCGAGAAATGGGAAGCCGAAGGCGAG
GTCTTCAAGGCCGAGTGGGCCAAGGAACTGCCCGAGGACGAGGAACTGACCGTCTATTGGTCCGGCGAACCGGGCGGCGA
GGATTCCTGGCTCGACATGTGCCGCGGCCCGCATCTTGCGAGCACGGGCAAGCTCGATCCACAGGCGTTCAAGCTGATGC
GCGTGGCCGGGGCCTATTGGCGCGGCGACCAGCGCAATCCGCAGCTGACGCGTATCTACGGCACCGGTTGGCTCAACAAG
AAGCAGCTCAACGCGCATCTCCACCGGCTGGAGGAAGCGGCCAAGCGCGACCACCGCAAGCTGGGCCGCGAGATGGATCT
CTTCCACCTGCAGGAAGAGGCGCATGGCAGCGTTTTCTGGCATCCGCAGGGCTACAAGATCTGGCGCGAGTTGGAGGCTT
ACATGCGCCGCAAGATGGACGGCGCGGGTTATCGCGAGATCAAGACGCCGCAGGTGATGGACGCGCGCCAATGGGAGCAG
TCCGGCCACTGGGGCAAATATCGCGAGAACATGTTCGTGATACCCGACGAGATCCCGAACACCGAGGACGAGGGCGAATT
GGTGTCGAAGGACGCCGACTGGATGGCGCTGAAGCCGATGAATTGCCCGGCGCACGTGCTCGTCTTCAAGCAGGGCATCA
CCTCCTATCGCGACCTGCCGATCCGGCTCGGCGAGATGGGCTGCTGTCACCGCAACGAGCCGCATGGCGCGCTCCACGGC
CTCATGCGCGTGCGCCAGTTCACGCAGGACGATGCGCATATCTTCTGCACCGAAGACCAGGTCGTCGAAGAGGTCCGCGC
TTTCTGCAAGCTGGCGGATGAAGTCTATCGCGACTTCGGATTCGATTACGACGTCAAGCTGGCGCTGCGTCCGGAACAGC
GGTTCGGCTCCGAAGAAGATTGGGACAAGGCCGAGCAGGAATTGCGCGATGCCGTCGCCGAAGCGGGCATGGCCAATGAC
GATTATGGTTGGGAGGAGCTGCCCGGCGAAGGTGCGTTCTATGCGCCCAAGCTCGAATGGCACCTGACCGATGCGATCGG
GCGGACCTGGCAGGTCGGCACGATCCAGGGAGACCGCGTGCTGCCCGAACGTCTCGACGCGACCTATGTCGGCGAGGATG
GCGGCAAGCACCGCCCGGTCATGCTGCACCGCGCGATCTTCGGCTCCTACGAGCGCTTCATCGGCATCCTGATCGAGCAT
TTTGCCGGCCGCCTGCCGGTATGGCTCGCCCCGACGCAGGCGGTCGTTGCGACCATCGTCAGCGATGCCGACGGCTATGC
GAAGGAGGCCGTGGCCAAGCTCGAGGCGGCGGGCATTCGCGTCGATGGCGACCTGCGCAACGAGAAGATCAACTTCAAGG
TGCGCGAACACTCGCTGGCCAAGGTCCCGCACCTGCTGGTGGTCGGCAAGCGCGAGGCAGAAGAAGGCACGGTCGCGGTC
CGCACGCTGGGCGAAAAGGAGCAGCAGGTGATGAGCCTCGATGATGCCATCGCCATGCTCAAGGACGCCGCGACACCGCC
AGACCTGCGCGACGGCTAA

Upstream 100 bases:

>100_bases
GCGAAGCCTCCCGGTTAATTCCCCTATCCCGATCGTCGCCCCTTTCGGCGCGCGCCCGCATGTGCAAAAGGCGCGCGCGA
CAATGCAGGACGTTTGAGCG

Downstream 100 bases:

>100_bases
AGCGCGTGGACCTGCTGGCGGACTATTCCGCGCTCCAGATCGGCGCAGCCTTGCTGGCGACCTTCGGCTCCGCCTTCGTG
CGCGGGCTGACCGGCTTCGG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MTELLKISLPDGSVREMEAGATPADVAAAIGPGLAKAALAAKVDGEVRDLNRPFEGDAELALITSRDEEEALELARHDYA
HVLAEAVQALWPGTQITFGPATDDGFYYDVKAPDSRDPFGMDDLPAIEEKMREIIKADKPLVREVWSREQLIEKWEAEGE
VFKAEWAKELPEDEELTVYWSGEPGGEDSWLDMCRGPHLASTGKLDPQAFKLMRVAGAYWRGDQRNPQLTRIYGTGWLNK
KQLNAHLHRLEEAAKRDHRKLGREMDLFHLQEEAHGSVFWHPQGYKIWRELEAYMRRKMDGAGYREIKTPQVMDARQWEQ
SGHWGKYRENMFVIPDEIPNTEDEGELVSKDADWMALKPMNCPAHVLVFKQGITSYRDLPIRLGEMGCCHRNEPHGALHG
LMRVRQFTQDDAHIFCTEDQVVEEVRAFCKLADEVYRDFGFDYDVKLALRPEQRFGSEEDWDKAEQELRDAVAEAGMAND
DYGWEELPGEGAFYAPKLEWHLTDAIGRTWQVGTIQGDRVLPERLDATYVGEDGGKHRPVMLHRAIFGSYERFIGILIEH
FAGRLPVWLAPTQAVVATIVSDADGYAKEAVAKLEAAGIRVDGDLRNEKINFKVREHSLAKVPHLLVVGKREAEEGTVAV
RTLGEKEQQVMSLDDAIAMLKDAATPPDLRDG

Sequences:

>Translated_672_residues
MTELLKISLPDGSVREMEAGATPADVAAAIGPGLAKAALAAKVDGEVRDLNRPFEGDAELALITSRDEEEALELARHDYA
HVLAEAVQALWPGTQITFGPATDDGFYYDVKAPDSRDPFGMDDLPAIEEKMREIIKADKPLVREVWSREQLIEKWEAEGE
VFKAEWAKELPEDEELTVYWSGEPGGEDSWLDMCRGPHLASTGKLDPQAFKLMRVAGAYWRGDQRNPQLTRIYGTGWLNK
KQLNAHLHRLEEAAKRDHRKLGREMDLFHLQEEAHGSVFWHPQGYKIWRELEAYMRRKMDGAGYREIKTPQVMDARQWEQ
SGHWGKYRENMFVIPDEIPNTEDEGELVSKDADWMALKPMNCPAHVLVFKQGITSYRDLPIRLGEMGCCHRNEPHGALHG
LMRVRQFTQDDAHIFCTEDQVVEEVRAFCKLADEVYRDFGFDYDVKLALRPEQRFGSEEDWDKAEQELRDAVAEAGMAND
DYGWEELPGEGAFYAPKLEWHLTDAIGRTWQVGTIQGDRVLPERLDATYVGEDGGKHRPVMLHRAIFGSYERFIGILIEH
FAGRLPVWLAPTQAVVATIVSDADGYAKEAVAKLEAAGIRVDGDLRNEKINFKVREHSLAKVPHLLVVGKREAEEGTVAV
RTLGEKEQQVMSLDDAIAMLKDAATPPDLRDG
>Mature_671_residues
TELLKISLPDGSVREMEAGATPADVAAAIGPGLAKAALAAKVDGEVRDLNRPFEGDAELALITSRDEEEALELARHDYAH
VLAEAVQALWPGTQITFGPATDDGFYYDVKAPDSRDPFGMDDLPAIEEKMREIIKADKPLVREVWSREQLIEKWEAEGEV
FKAEWAKELPEDEELTVYWSGEPGGEDSWLDMCRGPHLASTGKLDPQAFKLMRVAGAYWRGDQRNPQLTRIYGTGWLNKK
QLNAHLHRLEEAAKRDHRKLGREMDLFHLQEEAHGSVFWHPQGYKIWRELEAYMRRKMDGAGYREIKTPQVMDARQWEQS
GHWGKYRENMFVIPDEIPNTEDEGELVSKDADWMALKPMNCPAHVLVFKQGITSYRDLPIRLGEMGCCHRNEPHGALHGL
MRVRQFTQDDAHIFCTEDQVVEEVRAFCKLADEVYRDFGFDYDVKLALRPEQRFGSEEDWDKAEQELRDAVAEAGMANDD
YGWEELPGEGAFYAPKLEWHLTDAIGRTWQVGTIQGDRVLPERLDATYVGEDGGKHRPVMLHRAIFGSYERFIGILIEHF
AGRLPVWLAPTQAVVATIVSDADGYAKEAVAKLEAAGIRVDGDLRNEKINFKVREHSLAKVPHLLVVGKREAEEGTVAVR
TLGEKEQQVMSLDDAIAMLKDAATPPDLRDG

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=664, Percent_Identity=37.6506024096386, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI38202255, Length=660, Percent_Identity=38.030303030303, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI20070344, Length=662, Percent_Identity=37.1601208459215, Blast_Score=401, Evalue=1e-111,
Organism=Escherichia coli, GI1788013, Length=666, Percent_Identity=48.1981981981982, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=175, Percent_Identity=23.4285714285714, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71984184, Length=666, Percent_Identity=38.1381381381381, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71984192, Length=666, Percent_Identity=38.1381381381381, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322112, Length=678, Percent_Identity=37.905604719764, Blast_Score=453, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322654, Length=409, Percent_Identity=36.1858190709046, Blast_Score=262, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24583842, Length=663, Percent_Identity=37.7073906485671, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI28574141, Length=663, Percent_Identity=37.7073906485671, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24583839, Length=663, Percent_Identity=37.7073906485671, Blast_Score=444, Evalue=1e-124,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_ERYLH (Q2N6X5)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459363.1
- ProteinModelPortal:   Q2N6X5
- SMR:   Q2N6X5
- STRING:   Q2N6X5
- GeneID:   3868973
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_12370
- NMPDR:   fig|314225.3.peg.548
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   GFEYEVE
- PhylomeDB:   Q2N6X5
- ProtClustDB:   PRK00413
- BioCyc:   ELIT314225:ELI_12370-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 75949; Mature: 75818

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELLKISLPDGSVREMEAGATPADVAAAIGPGLAKAALAAKVDGEVRDLNRPFEGDAEL
CCCEEEEECCCCCHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHCCCCCCCCCCE
ALITSRDEEEALELARHDYAHVLAEAVQALWPGTQITFGPATDDGFYYDVKAPDSRDPFG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCCCC
MDDLPAIEEKMREIIKADKPLVREVWSREQLIEKWEAEGEVFKAEWAKELPEDEELTVYW
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCEEEEE
SGEPGGEDSWLDMCRGPHLASTGKLDPQAFKLMRVAGAYWRGDQRNPQLTRIYGTGWLNK
CCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCH
KQLNAHLHRLEEAAKRDHRKLGREMDLFHLQEEAHGSVFWHPQGYKIWRELEAYMRRKMD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCC
GAGYREIKTPQVMDARQWEQSGHWGKYRENMFVIPDEIPNTEDEGELVSKDADWMALKPM
CCCCCCCCCCCHHCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCEEEEECC
NCPAHVLVFKQGITSYRDLPIRLGEMGCCHRNEPHGALHGLMRVRQFTQDDAHIFCTEDQ
CCCCEEEEEHHCCHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHH
VVEEVRAFCKLADEVYRDFGFDYDVKLALRPEQRFGSEEDWDKAEQELRDAVAEAGMAND
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
DYGWEELPGEGAFYAPKLEWHLTDAIGRTWQVGTIQGDRVLPERLDATYVGEDGGKHRPV
CCCHHHCCCCCCEECCCEEEEEHHHHCCEEEEEECCCCCCCHHHCCCEEECCCCCCCCCH
MLHRAIFGSYERFIGILIEHFAGRLPVWLAPTQAVVATIVSDADGYAKEAVAKLEAAGIR
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHCCEE
VDGDLRNEKINFKVREHSLAKVPHLLVVGKREAEEGTVAVRTLGEKEQQVMSLDDAIAML
ECCCCCCCEEEEEEECCHHHHCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
KDAATPPDLRDG
HHCCCCCCCCCC
>Mature Secondary Structure 
TELLKISLPDGSVREMEAGATPADVAAAIGPGLAKAALAAKVDGEVRDLNRPFEGDAEL
CCEEEEECCCCCHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHCCCCCCCCCCE
ALITSRDEEEALELARHDYAHVLAEAVQALWPGTQITFGPATDDGFYYDVKAPDSRDPFG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCCCC
MDDLPAIEEKMREIIKADKPLVREVWSREQLIEKWEAEGEVFKAEWAKELPEDEELTVYW
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCEEEEE
SGEPGGEDSWLDMCRGPHLASTGKLDPQAFKLMRVAGAYWRGDQRNPQLTRIYGTGWLNK
CCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCH
KQLNAHLHRLEEAAKRDHRKLGREMDLFHLQEEAHGSVFWHPQGYKIWRELEAYMRRKMD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCC
GAGYREIKTPQVMDARQWEQSGHWGKYRENMFVIPDEIPNTEDEGELVSKDADWMALKPM
CCCCCCCCCCCHHCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCEEEEECC
NCPAHVLVFKQGITSYRDLPIRLGEMGCCHRNEPHGALHGLMRVRQFTQDDAHIFCTEDQ
CCCCEEEEEHHCCHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHH
VVEEVRAFCKLADEVYRDFGFDYDVKLALRPEQRFGSEEDWDKAEQELRDAVAEAGMAND
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
DYGWEELPGEGAFYAPKLEWHLTDAIGRTWQVGTIQGDRVLPERLDATYVGEDGGKHRPV
CCCHHHCCCCCCEECCCEEEEEHHHHCCEEEEEECCCCCCCHHHCCCEEECCCCCCCCCH
MLHRAIFGSYERFIGILIEHFAGRLPVWLAPTQAVVATIVSDADGYAKEAVAKLEAAGIR
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHCCEE
VDGDLRNEKINFKVREHSLAKVPHLLVVGKREAEEGTVAVRTLGEKEQQVMSLDDAIAML
ECCCCCCCEEEEEEECCHHHHCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
KDAATPPDLRDG
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA