Click here to switch to the map view.

The map label for this gene is rfbE [H]

Identifier: 85375263

GI number: 85375263

Start: 2461113

End: 2462603

Strand: Direct

Name: rfbE [H]

Synonym: ELI_12180

Alternate gene names: 85375263

Gene position: 2461113-2462603 (Clockwise)

Preceding gene: 85375262

Following gene: 85375264

Centisome position: 80.63

GC content: 66.8

Gene sequence:

>1491_bases
ATGCGGATCGCGACCGCTTTCGGGACCAATCTTGCCGGGCAGGTGCTGCGACTGGCCATCGCGGTCCTCACGGTTCCCCT
GTTCGCCGCCTATTTCGGCGCGGCACGGTTCGGCACCTATTACTTTCTGCTGGCGCTGATGCCCTATTTCCAGGTCCACG
CGCTGGCCGTCAGTCGCCAGGTCACGGCTCTTTCCGCGCGAGCCGAGGCGGGCGAAGGCCGGTTGCAGCAAATGCGGGCC
GAGCTCGGATCGTCTGTTGCGATCGGGACGATCATCAGCGTTGTGCTGACTACGGCATTTTTCGTCGGTTATCCATGGAT
GATGCAGGGGGTCGAGAGCGCCGAGGCACTCGCCGCGGAGATCGCCCGCGCGCGGCCATGGGCAGCGGCGATCGTCGTGC
TCGATGTCGTCATCGTCCTTCAGACCGCGCAGATGGAGGGCCGCAAGGCCTTCGCACCCTCCAACATCGCGCAGATCACC
GGCCAGGCCGCCACGCTTCTGCTGCCGCTGACGGCCTTCATCTACGACGGCAGCTTCGACCGGATGATTGCCGGGGTGGC
GCTGGCCCGGCTCGTCCAGGTGCTGGTCGGCCTGCGCTGGGCGCCTCTCTTGCCGATCCTCGCGCTTGTCCGCAGCGGCC
TCCGCATGCCGCGAAGCCTCGGCATCGATTTCGGGATGCTGACCGTTTCCGCCATTTGCAACGCCGTCAGTCGCACGCTC
GACCGGATCGTGATCGGGACGTCGGCCAGTGCGGCGGCGCTGTATTTCTATTCGGCTCCGATGGGCGGCACGGCGCGCCT
GTCCATGCTCGGCCGCTCGATGGGAGCGACCATGGCGCCCTTCTTCGCCACGGGCGAGGCCGACCAGCGCAGGGTGCAGG
CGGCAAAGGCGTCGAATGCGGTGCTGGCGCTCCTGTTCCCGGTCTGCGCCTTCGGCATCGTCAGTGCTCCCTTGCTCATG
ACTGTGTGGGTCGATGCCGAATTTGCCGAGCGCGGCACCACGTTCTTCCGGCTCGGGCTCATCGGAGTCATGGGATCGTC
GGTTGCGCAGGTCCCCGCCGTCCTGATGCAGTCCACCGGGCGGTCCAAATTCGTGACCGTGCAGCAATTGATCGAGCTGC
CGCCCTTTGCGTTCGCGCTGCTCTGGGCAAGCACCCACAATCTTGTCGCCGTGGCCTGGGTGGTGGCGCTGAGAATGGTT
GTTTCCGGCCTGGTCCAGATGACGGTCGCGGGCGTGCCGCGGCGCGCCATCGCGGCAGCGATCGCTTGCATCGCGGCGGC
CGTCGCGCTGAGCCTCGCGGCACCGTCGCTCGACCATCTGCCCCCTCTGTCGGCTCTGGCGGCGGGCGGGGGCGCAGCGC
TGGGTGCAAGCCTGATCGTCCACCTGCTTTCCCCGGATACAATGCGTTTCGCCTGCGAACTGATTGTCGCCCGCCTGCAT
CGCCGCCAGAAGACTGCGCTGCCATATCCGGAGCGAAACAATGGCCCGTAG

Upstream 100 bases:

>100_bases
GCCGGCCACAGTGCCCGGCTCGGCTGCTGGCTCGAAGGGGAGCGGTCGTCGTGAAACGGAGGCCCACGAGATGCCTCCGC
CAACGGGATTGCGCCTGACC

Downstream 100 bases:

>100_bases
CGGCGACCAAACGCCACCGGGGACGCGCGGATTCGTCGCAGCGACGGTGTTTGCTTTTGCAACGCTCCTGCTGGTCGGGG
GCACCGCCGCACCCCTCAAC

Product: polysaccharide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MRIATAFGTNLAGQVLRLAIAVLTVPLFAAYFGAARFGTYYFLLALMPYFQVHALAVSRQVTALSARAEAGEGRLQQMRA
ELGSSVAIGTIISVVLTTAFFVGYPWMMQGVESAEALAAEIARARPWAAAIVVLDVVIVLQTAQMEGRKAFAPSNIAQIT
GQAATLLLPLTAFIYDGSFDRMIAGVALARLVQVLVGLRWAPLLPILALVRSGLRMPRSLGIDFGMLTVSAICNAVSRTL
DRIVIGTSASAAALYFYSAPMGGTARLSMLGRSMGATMAPFFATGEADQRRVQAAKASNAVLALLFPVCAFGIVSAPLLM
TVWVDAEFAERGTTFFRLGLIGVMGSSVAQVPAVLMQSTGRSKFVTVQQLIELPPFAFALLWASTHNLVAVAWVVALRMV
VSGLVQMTVAGVPRRAIAAAIACIAAAVALSLAAPSLDHLPPLSALAAGGGAALGASLIVHLLSPDTMRFACELIVARLH
RRQKTALPYPERNNGP

Sequences:

>Translated_496_residues
MRIATAFGTNLAGQVLRLAIAVLTVPLFAAYFGAARFGTYYFLLALMPYFQVHALAVSRQVTALSARAEAGEGRLQQMRA
ELGSSVAIGTIISVVLTTAFFVGYPWMMQGVESAEALAAEIARARPWAAAIVVLDVVIVLQTAQMEGRKAFAPSNIAQIT
GQAATLLLPLTAFIYDGSFDRMIAGVALARLVQVLVGLRWAPLLPILALVRSGLRMPRSLGIDFGMLTVSAICNAVSRTL
DRIVIGTSASAAALYFYSAPMGGTARLSMLGRSMGATMAPFFATGEADQRRVQAAKASNAVLALLFPVCAFGIVSAPLLM
TVWVDAEFAERGTTFFRLGLIGVMGSSVAQVPAVLMQSTGRSKFVTVQQLIELPPFAFALLWASTHNLVAVAWVVALRMV
VSGLVQMTVAGVPRRAIAAAIACIAAAVALSLAAPSLDHLPPLSALAAGGGAALGASLIVHLLSPDTMRFACELIVARLH
RRQKTALPYPERNNGP
>Mature_496_residues
MRIATAFGTNLAGQVLRLAIAVLTVPLFAAYFGAARFGTYYFLLALMPYFQVHALAVSRQVTALSARAEAGEGRLQQMRA
ELGSSVAIGTIISVVLTTAFFVGYPWMMQGVESAEALAAEIARARPWAAAIVVLDVVIVLQTAQMEGRKAFAPSNIAQIT
GQAATLLLPLTAFIYDGSFDRMIAGVALARLVQVLVGLRWAPLLPILALVRSGLRMPRSLGIDFGMLTVSAICNAVSRTL
DRIVIGTSASAAALYFYSAPMGGTARLSMLGRSMGATMAPFFATGEADQRRVQAAKASNAVLALLFPVCAFGIVSAPLLM
TVWVDAEFAERGTTFFRLGLIGVMGSSVAQVPAVLMQSTGRSKFVTVQQLIELPPFAFALLWASTHNLVAVAWVVALRMV
VSGLVQMTVAGVPRRAIAAAIACIAAAVALSLAAPSLDHLPPLSALAAGGGAALGASLIVHLLSPDTMRFACELIVARLH
RRQKTALPYPERNNGP

Specific function: Could be an O-antigen transporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 52240; Mature: 52240

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATAFGTNLAGQVLRLAIAVLTVPLFAAYFGAARFGTYYFLLALMPYFQVHALAVSRQ
CEEECHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTALSARAEAGEGRLQQMRAELGSSVAIGTIISVVLTTAFFVGYPWMMQGVESAEALAAE
HHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IARARPWAAAIVVLDVVIVLQTAQMEGRKAFAPSNIAQITGQAATLLLPLTAFIYDGSFD
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCHH
RMIAGVALARLVQVLVGLRWAPLLPILALVRSGLRMPRSLGIDFGMLTVSAICNAVSRTL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHH
DRIVIGTSASAAALYFYSAPMGGTARLSMLGRSMGATMAPFFATGEADQRRVQAAKASNA
HHHHCCCCCCHHEEEEEECCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCH
VLALLFPVCAFGIVSAPLLMTVWVDAEFAERGTTFFRLGLIGVMGSSVAQVPAVLMQSTG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCC
RSKFVTVQQLIELPPFAFALLWASTHNLVAVAWVVALRMVVSGLVQMTVAGVPRRAIAAA
CCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IACIAAAVALSLAAPSLDHLPPLSALAAGGGAALGASLIVHLLSPDTMRFACELIVARLH
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RRQKTALPYPERNNGP
HHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRIATAFGTNLAGQVLRLAIAVLTVPLFAAYFGAARFGTYYFLLALMPYFQVHALAVSRQ
CEEECHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTALSARAEAGEGRLQQMRAELGSSVAIGTIISVVLTTAFFVGYPWMMQGVESAEALAAE
HHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IARARPWAAAIVVLDVVIVLQTAQMEGRKAFAPSNIAQITGQAATLLLPLTAFIYDGSFD
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCHH
RMIAGVALARLVQVLVGLRWAPLLPILALVRSGLRMPRSLGIDFGMLTVSAICNAVSRTL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHH
DRIVIGTSASAAALYFYSAPMGGTARLSMLGRSMGATMAPFFATGEADQRRVQAAKASNA
HHHHCCCCCCHHEEEEEECCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCH
VLALLFPVCAFGIVSAPLLMTVWVDAEFAERGTTFFRLGLIGVMGSSVAQVPAVLMQSTG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCC
RSKFVTVQQLIELPPFAFALLWASTHNLVAVAWVVALRMVVSGLVQMTVAGVPRRAIAAA
CCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IACIAAAVALSLAAPSLDHLPPLSALAAGGGAALGASLIVHLLSPDTMRFACELIVARLH
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RRQKTALPYPERNNGP
HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7507920; 12384590; 12704152 [H]