Click here to switch to the map view.

The map label for this gene is phoA [H]

Identifier: 85375220

GI number: 85375220

Start: 2414154

End: 2415584

Strand: Reverse

Name: phoA [H]

Synonym: ELI_11965

Alternate gene names: 85375220

Gene position: 2415584-2414154 (Counterclockwise)

Preceding gene: 85375224

Following gene: 85375214

Centisome position: 79.14

GC content: 66.18

Gene sequence:

>1431_bases
ATGATCCAGTCTCGCCCGGCCCTCGCCGCCCTTTTTGCCACTTTCGCGCTGCCACTGGCGGGTTGCGGCACAGCCTATGC
GCAAGACGACGTGACGAGCGCGCCAGCGCCAGTGCAACAGGCTGCAGAGACGCAAGAATCGGGCAAGGCCAAGAATGTCA
TTCTCTTCATAGGTGACGGGATGGGTGTCTCGACCGTGACCGCCGCGCGCATCTATGCCGGTCAGAAACTCGGCCAGACC
GGCGAGGAATATATCCTGCCGTTCGAACGCTTCGAAAATGTCGCGCTGGTCAAGACCTACAACACCAACGCGCAGGTACC
CGATAGCGCGGGCACGGCAACGGCGATGCATTCTGGCAAGAAGACCAAGATCGGCTTTCTCGGCATCGGGCCGGAGGCAC
CGCGCGCCGATTGCGTGGCGGGGCAGCAATACCACCTGCCGCTGATCGGCGAGCAGGCGAAGGAGCGCGGCCTGGCGGTC
GGCGTGGTCAGCACGGCGCGCATCACCCATGCGACGCCGGCCTCGGTCTATGCCCGCGCGACCGAGCGCAACTGGGAAGC
CGATGCCTCGATGCCGGAAGACCAGCGCGGGCTCGGCTGCGACGACATTGCGACGCAATTGGTGAATTTCGATTTCGATG
TCGCGCTCGGCGGCGGCACCGCGTCCTTCTTCGGCGCCGACAACGGCGGCCGCAGGCTCGACGCCGCCGCGGACCTCCCC
GGCGCGTGGGTGGCGCGGACAGGCGGCACCTATGTGACCGACGCCAACGAATTGAGCCTCGCCCCGGCGGGGAAGCCGGT
CCTCGGGCTCTTTTCCCCGAGCCACATGACCTACATGGCGGAGAAAGCCGCCGACAATGGCGAGCCGACGCTCACCGCGA
TGACGCGCGAAGCGATCCAGCGGCTCGCCAAGGACCCCGACGGCTATTACCTGATGGTCGAAAGCGGGAGGATCGACCAC
GGCCACCACTGGGGCAAGGCCGGGGTTGCGCTGGAGGAAGCGGTCGAATTCGCCCGCGCGATCCAGTGGGCGATCGAAAA
CACCGATCCGGAAGAGACGCTGATCCTCGTCACCGCGGACCACAGCCATGTCTTTACCATCGCCGGCTATCCCAAGCGCG
GCAATCCCATCCTCGGCCTGGTGGTCCCGCCGGAGGGAGAAGGTGACGGCGGCCCGATTCTTGCCGAGGACGGCAAGCCC
TATACGACCTTGGGTTACGCAAACGGCCCCGGCGCTGTCTCAGGCGAGCGACCCGTCCCCGAAACGGGCGTTATGGCGAC
ACAGCAGGCTTTGGTGCCGACCGGCAGCGAGACCCATGCGGGCGAAGACGTGCCGCTCTATGCGCAGGGGCCCGGCGCCG
AGCGCGCCCGCGGCGTCATCGAGCAGAATGTGGTCTACGACATCATGGTCGAGGCACTAGGATGGAAGTGA

Upstream 100 bases:

>100_bases
CGCGCCCCGCCCAAGCAAGGGAGCGCGGGCGGGTTTTCGCTTTGCTGTCACAACGAAGGGCGATAGCACGGCGACACGAT
CCGAAATGCGGAGATATTTG

Downstream 100 bases:

>100_bases
ACAATTAGGCCCATTATCTTATAAGTATCGCTGAAGCACGGCGCATAGCGCCGCAAGGCCGACTGGCCGCCCGCAGCGAT
TGGGTCCTGAGCCTGCCGAA

Product: alkaline phosphatase family protein

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MIQSRPALAALFATFALPLAGCGTAYAQDDVTSAPAPVQQAAETQESGKAKNVILFIGDGMGVSTVTAARIYAGQKLGQT
GEEYILPFERFENVALVKTYNTNAQVPDSAGTATAMHSGKKTKIGFLGIGPEAPRADCVAGQQYHLPLIGEQAKERGLAV
GVVSTARITHATPASVYARATERNWEADASMPEDQRGLGCDDIATQLVNFDFDVALGGGTASFFGADNGGRRLDAAADLP
GAWVARTGGTYVTDANELSLAPAGKPVLGLFSPSHMTYMAEKAADNGEPTLTAMTREAIQRLAKDPDGYYLMVESGRIDH
GHHWGKAGVALEEAVEFARAIQWAIENTDPEETLILVTADHSHVFTIAGYPKRGNPILGLVVPPEGEGDGGPILAEDGKP
YTTLGYANGPGAVSGERPVPETGVMATQQALVPTGSETHAGEDVPLYAQGPGAERARGVIEQNVVYDIMVEALGWK

Sequences:

>Translated_476_residues
MIQSRPALAALFATFALPLAGCGTAYAQDDVTSAPAPVQQAAETQESGKAKNVILFIGDGMGVSTVTAARIYAGQKLGQT
GEEYILPFERFENVALVKTYNTNAQVPDSAGTATAMHSGKKTKIGFLGIGPEAPRADCVAGQQYHLPLIGEQAKERGLAV
GVVSTARITHATPASVYARATERNWEADASMPEDQRGLGCDDIATQLVNFDFDVALGGGTASFFGADNGGRRLDAAADLP
GAWVARTGGTYVTDANELSLAPAGKPVLGLFSPSHMTYMAEKAADNGEPTLTAMTREAIQRLAKDPDGYYLMVESGRIDH
GHHWGKAGVALEEAVEFARAIQWAIENTDPEETLILVTADHSHVFTIAGYPKRGNPILGLVVPPEGEGDGGPILAEDGKP
YTTLGYANGPGAVSGERPVPETGVMATQQALVPTGSETHAGEDVPLYAQGPGAERARGVIEQNVVYDIMVEALGWK
>Mature_476_residues
MIQSRPALAALFATFALPLAGCGTAYAQDDVTSAPAPVQQAAETQESGKAKNVILFIGDGMGVSTVTAARIYAGQKLGQT
GEEYILPFERFENVALVKTYNTNAQVPDSAGTATAMHSGKKTKIGFLGIGPEAPRADCVAGQQYHLPLIGEQAKERGLAV
GVVSTARITHATPASVYARATERNWEADASMPEDQRGLGCDDIATQLVNFDFDVALGGGTASFFGADNGGRRLDAAADLP
GAWVARTGGTYVTDANELSLAPAGKPVLGLFSPSHMTYMAEKAADNGEPTLTAMTREAIQRLAKDPDGYYLMVESGRIDH
GHHWGKAGVALEEAVEFARAIQWAIENTDPEETLILVTADHSHVFTIAGYPKRGNPILGLVVPPEGEGDGGPILAEDGKP
YTTLGYANGPGAVSGERPVPETGVMATQQALVPTGSETHAGEDVPLYAQGPGAERARGVIEQNVVYDIMVEALGWK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=464, Percent_Identity=44.3965517241379, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI94721246, Length=442, Percent_Identity=44.5701357466063, Blast_Score=319, Evalue=5e-87,
Organism=Homo sapiens, GI116734717, Length=467, Percent_Identity=43.4689507494647, Blast_Score=315, Evalue=8e-86,
Organism=Homo sapiens, GI157266296, Length=445, Percent_Identity=44.0449438202247, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI294660770, Length=444, Percent_Identity=43.6936936936937, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI294660772, Length=409, Percent_Identity=41.320293398533, Blast_Score=260, Evalue=2e-69,
Organism=Escherichia coli, GI48994877, Length=465, Percent_Identity=31.3978494623656, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320689, Length=351, Percent_Identity=30.1994301994302, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21355981, Length=455, Percent_Identity=40.6593406593407, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI21355151, Length=450, Percent_Identity=40.8888888888889, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24651554, Length=477, Percent_Identity=40.6708595387841, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24657842, Length=453, Percent_Identity=40.1766004415011, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI21355149, Length=479, Percent_Identity=38.2045929018789, Blast_Score=277, Evalue=9e-75,
Organism=Drosophila melanogaster, GI85725200, Length=446, Percent_Identity=40.1345291479821, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI85815833, Length=446, Percent_Identity=40.1345291479821, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI21358067, Length=512, Percent_Identity=36.71875, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24651556, Length=458, Percent_Identity=40.174672489083, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24657835, Length=448, Percent_Identity=38.6160714285714, Blast_Score=261, Evalue=6e-70,
Organism=Drosophila melanogaster, GI19921912, Length=468, Percent_Identity=38.6752136752137, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24657827, Length=449, Percent_Identity=39.1982182628062, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI18859923, Length=436, Percent_Identity=37.1559633027523, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24585213, Length=473, Percent_Identity=36.7864693446089, Blast_Score=239, Evalue=4e-63,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 49918; Mature: 49918

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSRPALAALFATFALPLAGCGTAYAQDDVTSAPAPVQQAAETQESGKAKNVILFIGDG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECC
MGVSTVTAARIYAGQKLGQTGEEYILPFERFENVALVKTYNTNAQVPDSAGTATAMHSGK
CCCHHHHHHHHHHCHHHCCCCHHEECCHHHCCCEEEEEEECCCCCCCCCCCCCHHHCCCC
KTKIGFLGIGPEAPRADCVAGQQYHLPLIGEQAKERGLAVGVVSTARITHATPASVYARA
CEEEEEEECCCCCCCCHHCCCCEEECCCCCCHHHHCCEEEEEEEEEEEECCCCHHHEEEH
TERNWEADASMPEDQRGLGCDDIATQLVNFDFDVALGGGTASFFGADNGGRRLDAAADLP
HCCCCCCCCCCCCHHCCCCHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCEECHHCCCC
GAWVARTGGTYVTDANELSLAPAGKPVLGLFSPSHMTYMAEKAADNGEPTLTAMTREAIQ
CCEEEECCCEEEECCCCEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHH
RLAKDPDGYYLMVESGRIDHGHHWGKAGVALEEAVEFARAIQWAIENTDPEETLILVTAD
HHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
HSHVFTIAGYPKRGNPILGLVVPPEGEGDGGPILAEDGKPYTTLGYANGPGAVSGERPVP
CCEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCC
ETGVMATQQALVPTGSETHAGEDVPLYAQGPGAERARGVIEQNVVYDIMVEALGWK
CCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIQSRPALAALFATFALPLAGCGTAYAQDDVTSAPAPVQQAAETQESGKAKNVILFIGDG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECC
MGVSTVTAARIYAGQKLGQTGEEYILPFERFENVALVKTYNTNAQVPDSAGTATAMHSGK
CCCHHHHHHHHHHCHHHCCCCHHEECCHHHCCCEEEEEEECCCCCCCCCCCCCHHHCCCC
KTKIGFLGIGPEAPRADCVAGQQYHLPLIGEQAKERGLAVGVVSTARITHATPASVYARA
CEEEEEEECCCCCCCCHHCCCCEEECCCCCCHHHHCCEEEEEEEEEEEECCCCHHHEEEH
TERNWEADASMPEDQRGLGCDDIATQLVNFDFDVALGGGTASFFGADNGGRRLDAAADLP
HCCCCCCCCCCCCHHCCCCHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCEECHHCCCC
GAWVARTGGTYVTDANELSLAPAGKPVLGLFSPSHMTYMAEKAADNGEPTLTAMTREAIQ
CCEEEECCCEEEECCCCEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHH
RLAKDPDGYYLMVESGRIDHGHHWGKAGVALEEAVEFARAIQWAIENTDPEETLILVTAD
HHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
HSHVFTIAGYPKRGNPILGLVVPPEGEGDGGPILAEDGKPYTTLGYANGPGAVSGERPVP
CCEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCC
ETGVMATQQALVPTGSETHAGEDVPLYAQGPGAERARGVIEQNVVYDIMVEALGWK
CCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]