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The map label for this gene is fadH [C]

Identifier: 85375203

GI number: 85375203

Start: 2396455

End: 2397732

Strand: Reverse

Name: fadH [C]

Synonym: ELI_11880

Alternate gene names: 85375203

Gene position: 2397732-2396455 (Counterclockwise)

Preceding gene: 85375204

Following gene: 85375201

Centisome position: 78.55

GC content: 66.35

Gene sequence:

>1278_bases
ATGACCACTTCGCCGCTTTTCGAACCACTCACCCTGCCCAATGGCACGACACTGAAAAACCGGCTGTGCAAAGCGGCGAT
GGAGGAGAACCTCGCTGTCCAGCCCGGGCAGTATCCGGGCGAAAAGCTGTTTACGCTCTACGAACGCTGGGCGCGTGGCG
GGGTCGGGCTGATCCTGTCGGGCAATGTCATGGTCGATCCTGGCGCGCTGACCGGGCCGGGCGCGGTGATCCTGGAGAAA
GGCACCGACCTCACCCCGTTCAAGAACTGGGCGCAGATCGGCAAATCGGGCGGCGGGCAGTTCTGGCTGCAGATCAGCCA
TCCGGGGCGGCAACTCTACAAAAGCATGGGCGAAACGGCGGTGTCTCCGTCGGACGTCGCGCTCGATCTCGGCAAGTTTT
CCTCGCTCTTCGCCGAGGTGCGCGCGCTCGAGGCATCGGAAATCGAGACAATCATCACCCGCTTCGCCGACACCGCCGAG
CAGGCGCAGGAAGCGGGCTTCGACGGCGTGCAGATTCACGGTGCGCATGGCTATCTCGTCAGCCAGTTCCTGTCGCCGCT
GACCAACAGGCGGAGCGACGAGTGGGGCGGAAGCCTGGAGAACCGCGCGCGCTTCCTCCTCAGGATCGTCGAGGCCTGCC
GCGCGCGGGTGAAGCCTGAATTCGGTATCGGGGTGAAGCTCAACTCGGCCGATTTCCAGCGCGGAGGCTTCGCCTTCGAG
GACGCGCGGCAAGTGGTCGAATGGCTGAATGGCAAGGACGTCGATTTCGTGGAGCTATCGGGCGGCAGTTACGAGAGCCC
GGCCATGCAGGGCAATCCGCAGGAGGAAGGCGACGCGCCCAAATCCTCCACCGAAGCGCGCGAGGCCTATTTCATCGACT
TCGCCCGCGACATCGCCAGGGTCGCCGAGATGCCGATCATGGTGACGGGCGGCATCACGAAGCTGGCGACCGCCGAGGCC
GCGCTGGCCCACGACGCGCACGGCTTCGGCGTCGAACTGCTCGGCCTGGCGCGGGCCATGGCGAGCGACCCCTTCCTGCC
GCGCCATTGGCAAGTCGGCGAGAATACGGACGTCGCGCTGCCCGAGGTCGGTTGGAAAAACCGCTCGCTGTCCGGTCTCG
CCACGATGGGTCTGACCAAGGCGCAGATCGAGCGGATGGCGAAGGGCAAGAACCCCGGCGACGGCGGTTCCCCCGCGCTG
GCGCTGGCGGCGGACCAATACCGCGCCGCGCGCCGGGCCAAACGCTATCGCAAGTGGCGGGCGGCAAACGCCGCCTGA

Upstream 100 bases:

>100_bases
CGGCGACGACCTGGGCCATGCTCGACCGCGAAACGCAGCGTCTGGTGCGCGTCCGGCCCGACGTGATAGCGCCGTTCAGG
ACGGATCAGGGGAAAAACGC

Downstream 100 bases:

>100_bases
TCGCTAGTCGGCGAGCACCTCGGCCGTGCTGCGATAGTCGTAGCCCAGTTCTTCCGCCACCGCCTCGTAGGTCACATGGC
CGGCGTGGACGTTGAGCCCT

Product: FMN oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTTSPLFEPLTLPNGTTLKNRLCKAAMEENLAVQPGQYPGEKLFTLYERWARGGVGLILSGNVMVDPGALTGPGAVILEK
GTDLTPFKNWAQIGKSGGGQFWLQISHPGRQLYKSMGETAVSPSDVALDLGKFSSLFAEVRALEASEIETIITRFADTAE
QAQEAGFDGVQIHGAHGYLVSQFLSPLTNRRSDEWGGSLENRARFLLRIVEACRARVKPEFGIGVKLNSADFQRGGFAFE
DARQVVEWLNGKDVDFVELSGGSYESPAMQGNPQEEGDAPKSSTEAREAYFIDFARDIARVAEMPIMVTGGITKLATAEA
ALAHDAHGFGVELLGLARAMASDPFLPRHWQVGENTDVALPEVGWKNRSLSGLATMGLTKAQIERMAKGKNPGDGGSPAL
ALAADQYRAARRAKRYRKWRAANAA

Sequences:

>Translated_425_residues
MTTSPLFEPLTLPNGTTLKNRLCKAAMEENLAVQPGQYPGEKLFTLYERWARGGVGLILSGNVMVDPGALTGPGAVILEK
GTDLTPFKNWAQIGKSGGGQFWLQISHPGRQLYKSMGETAVSPSDVALDLGKFSSLFAEVRALEASEIETIITRFADTAE
QAQEAGFDGVQIHGAHGYLVSQFLSPLTNRRSDEWGGSLENRARFLLRIVEACRARVKPEFGIGVKLNSADFQRGGFAFE
DARQVVEWLNGKDVDFVELSGGSYESPAMQGNPQEEGDAPKSSTEAREAYFIDFARDIARVAEMPIMVTGGITKLATAEA
ALAHDAHGFGVELLGLARAMASDPFLPRHWQVGENTDVALPEVGWKNRSLSGLATMGLTKAQIERMAKGKNPGDGGSPAL
ALAADQYRAARRAKRYRKWRAANAA
>Mature_424_residues
TTSPLFEPLTLPNGTTLKNRLCKAAMEENLAVQPGQYPGEKLFTLYERWARGGVGLILSGNVMVDPGALTGPGAVILEKG
TDLTPFKNWAQIGKSGGGQFWLQISHPGRQLYKSMGETAVSPSDVALDLGKFSSLFAEVRALEASEIETIITRFADTAEQ
AQEAGFDGVQIHGAHGYLVSQFLSPLTNRRSDEWGGSLENRARFLLRIVEACRARVKPEFGIGVKLNSADFQRGGFAFED
ARQVVEWLNGKDVDFVELSGGSYESPAMQGNPQEEGDAPKSSTEAREAYFIDFARDIARVAEMPIMVTGGITKLATAEAA
LAHDAHGFGVELLGLARAMASDPFLPRHWQVGENTDVALPEVGWKNRSLSGLATMGLTKAQIERMAKGKNPGDGGSPALA
LAADQYRAARRAKRYRKWRAANAA

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=361, Percent_Identity=29.0858725761773, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787939, Length=283, Percent_Identity=31.4487632508834, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17564188, Length=360, Percent_Identity=37.5, Blast_Score=221, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI17540738, Length=404, Percent_Identity=36.1386138613861, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17565138, Length=403, Percent_Identity=37.2208436724566, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI17559802, Length=363, Percent_Identity=37.465564738292, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17559804, Length=360, Percent_Identity=35.5555555555556, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI72001454, Length=362, Percent_Identity=35.9116022099448, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17566914, Length=372, Percent_Identity=35.4838709677419, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17566916, Length=362, Percent_Identity=31.767955801105, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6325086, Length=233, Percent_Identity=29.1845493562232, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321973, Length=88, Percent_Identity=43.1818181818182, Blast_Score=83, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 45943; Mature: 45812

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSPLFEPLTLPNGTTLKNRLCKAAMEENLAVQPGQYPGEKLFTLYERWARGGVGLILS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEE
GNVMVDPGALTGPGAVILEKGTDLTPFKNWAQIGKSGGGQFWLQISHPGRQLYKSMGETA
CCEEECCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCC
VSPSDVALDLGKFSSLFAEVRALEASEIETIITRFADTAEQAQEAGFDGVQIHGAHGYLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
SQFLSPLTNRRSDEWGGSLENRARFLLRIVEACRARVKPEFGIGVKLNSADFQRGGFAFE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHH
DARQVVEWLNGKDVDFVELSGGSYESPAMQGNPQEEGDAPKSSTEAREAYFIDFARDIAR
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAEMPIMVTGGITKLATAEAALAHDAHGFGVELLGLARAMASDPFLPRHWQVGENTDVAL
HHHCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PEVGWKNRSLSGLATMGLTKAQIERMAKGKNPGDGGSPALALAADQYRAARRAKRYRKWR
CCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
AANAA
CCCCC
>Mature Secondary Structure 
TTSPLFEPLTLPNGTTLKNRLCKAAMEENLAVQPGQYPGEKLFTLYERWARGGVGLILS
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEE
GNVMVDPGALTGPGAVILEKGTDLTPFKNWAQIGKSGGGQFWLQISHPGRQLYKSMGETA
CCEEECCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCC
VSPSDVALDLGKFSSLFAEVRALEASEIETIITRFADTAEQAQEAGFDGVQIHGAHGYLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
SQFLSPLTNRRSDEWGGSLENRARFLLRIVEACRARVKPEFGIGVKLNSADFQRGGFAFE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHH
DARQVVEWLNGKDVDFVELSGGSYESPAMQGNPQEEGDAPKSSTEAREAYFIDFARDIAR
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAEMPIMVTGGITKLATAEAALAHDAHGFGVELLGLARAMASDPFLPRHWQVGENTDVAL
HHHCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PEVGWKNRSLSGLATMGLTKAQIERMAKGKNPGDGGSPALALAADQYRAARRAKRYRKWR
CCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
AANAA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]