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The map label for this gene is argB

Identifier: 85375135

GI number: 85375135

Start: 2332050

End: 2332949

Strand: Reverse

Name: argB

Synonym: ELI_11540

Alternate gene names: 85375135

Gene position: 2332949-2332050 (Counterclockwise)

Preceding gene: 85375136

Following gene: 85375134

Centisome position: 76.43

GC content: 65.22

Gene sequence:

>900_bases
ATGACTGCCACGGAAAAAACCGAACTGGCGAAAGCCGAAGTGCTGATCGAAGCGCTGCCCTATTTCCAGCGCTATGCCGG
CCGCACCTTCGTGGTCAAATACGGCGGCCACGCCATGGGCGATCCGAAGGCTGCACGGGAGTTCGCCGAGGATATCGTCC
TGCTGAAAGCTGTCGGAATCAATCCGGTGGTGGTGCATGGCGGCGGGCCGCAGATCGGCGCCATGCTGAAGCGCCTGGGC
GTCGAAAGCACTTTCGTCGACGGGCTTCGCGTGACCGACAAGGCGACGGCCGAAGTCGCGGAGATGGTTCTGTCGGGCGC
GATCAACAAGGAACTGGTCGGCTGGATCGCGCAGGCCGGCGGCAAGGCCATGGGTGTATCGGGCAAGGACGGATCGCTGG
TGACCGCGACCAAGCTCGAGCGGACGACGCGCGATCCGGAGAGCCAGATCGAACAGATACTCGATCTCGGCTTCGTCGGC
GAACCGACCCATGTCGATACGACGATCCTCGATACCGCGGTCAGCGCGGGGATGATTCCGGTCGTCGCGCCGATCGGTGC
GGGCGAGGACGGCCACACCTACAACATCAATGCCGACACCATGGCGGGCGCGATCGCGGCGGCGCTGGGCGCCGCGAGGC
TGTTCCTGCTCACCGCTGTGAAGGGCGTGCTCGACAAGCAAGGCGGGCTGCTGACCGACCTGACCCCCGCACAGATTGCG
AAGCTGAAGGACGACGGCACGATCTCCGGCGGGATGATCCCCAAGCTCGACACCTGCGTCCACGCAGTCGAGGCCGGTTG
CGAGGCGGCAGTCGTGCTCGACGGCCGCGTACCGCACGCAATGCTGCTGGAATTCTTTACGGCGCGCGGCGCGGGAACGC
TGATCAGGGCCGACGATTGA

Upstream 100 bases:

>100_bases
GACACCGCGACCGAGTAAATCGCCGCACGCAAGAACTTCCCAGTGAGCGGAAGAGAGGCTAGGCGCTGCTCCTGCAATTG
ACGGGAGCGGCGCTTTTGCC

Downstream 100 bases:

>100_bases
ACCGTTGATTGAACCGGCGCGCCATGCGTATTATGTTGTTGATACACCCTGCGGCTGCTAGCCAAAGGATAAGGACTGCC
CCACCGCCTGTGCGAGAGGT

Product: acetylglutamate kinase

Products: NA

Alternate protein names: N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AGK

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTATEKTELAKAEVLIEALPYFQRYAGRTFVVKYGGHAMGDPKAAREFAEDIVLLKAVGINPVVVHGGGPQIGAMLKRLG
VESTFVDGLRVTDKATAEVAEMVLSGAINKELVGWIAQAGGKAMGVSGKDGSLVTATKLERTTRDPESQIEQILDLGFVG
EPTHVDTTILDTAVSAGMIPVVAPIGAGEDGHTYNINADTMAGAIAAALGAARLFLLTAVKGVLDKQGGLLTDLTPAQIA
KLKDDGTISGGMIPKLDTCVHAVEAGCEAAVVLDGRVPHAMLLEFFTARGAGTLIRADD

Sequences:

>Translated_299_residues
MTATEKTELAKAEVLIEALPYFQRYAGRTFVVKYGGHAMGDPKAAREFAEDIVLLKAVGINPVVVHGGGPQIGAMLKRLG
VESTFVDGLRVTDKATAEVAEMVLSGAINKELVGWIAQAGGKAMGVSGKDGSLVTATKLERTTRDPESQIEQILDLGFVG
EPTHVDTTILDTAVSAGMIPVVAPIGAGEDGHTYNINADTMAGAIAAALGAARLFLLTAVKGVLDKQGGLLTDLTPAQIA
KLKDDGTISGGMIPKLDTCVHAVEAGCEAAVVLDGRVPHAMLLEFFTARGAGTLIRADD
>Mature_298_residues
TATEKTELAKAEVLIEALPYFQRYAGRTFVVKYGGHAMGDPKAAREFAEDIVLLKAVGINPVVVHGGGPQIGAMLKRLGV
ESTFVDGLRVTDKATAEVAEMVLSGAINKELVGWIAQAGGKAMGVSGKDGSLVTATKLERTTRDPESQIEQILDLGFVGE
PTHVDTTILDTAVSAGMIPVVAPIGAGEDGHTYNINADTMAGAIAAALGAARLFLLTAVKGVLDKQGGLLTDLTPAQIAK
LKDDGTISGGMIPKLDTCVHAVEAGCEAAVVLDGRVPHAMLLEFFTARGAGTLIRADD

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetylglutamate kinase family

Homologues:

Organism=Escherichia coli, GI1789181, Length=302, Percent_Identity=24.8344370860927, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693208, Length=234, Percent_Identity=29.4871794871795, Blast_Score=86, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320913, Length=291, Percent_Identity=25.085910652921, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ARGB_ERYLH (Q2N7E1)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459197.1
- HSSP:   Q9HTN2
- ProteinModelPortal:   Q2N7E1
- SMR:   Q2N7E1
- STRING:   Q2N7E1
- GeneID:   3869794
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_11540
- NMPDR:   fig|314225.3.peg.713
- eggNOG:   COG0548
- HOGENOM:   HBG497643
- OMA:   MDIVEMV
- PhylomeDB:   Q2N7E1
- ProtClustDB:   PRK00942
- BioCyc:   ELIT314225:ELI_11540-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00082_B
- InterPro:   IPR004662
- InterPro:   IPR001048
- InterPro:   IPR001057
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000728
- PRINTS:   PR00474
- TIGRFAMs:   TIGR00761

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.8

Molecular weight: Translated: 31002; Mature: 30871

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: BINDING 90-90 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATEKTELAKAEVLIEALPYFQRYAGRTFVVKYGGHAMGDPKAAREFAEDIVLLKAVGI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHEECCC
NPVVVHGGGPQIGAMLKRLGVESTFVDGLRVTDKATAEVAEMVLSGAINKELVGWIAQAG
CEEEEECCCCHHHHHHHHHCCCHHHHCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCC
GKAMGVSGKDGSLVTATKLERTTRDPESQIEQILDLGFVGEPTHVDTTILDTAVSAGMIP
CCEEECCCCCCCEEEHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCE
VVAPIGAGEDGHTYNINADTMAGAIAAALGAARLFLLTAVKGVLDKQGGLLTDLTPAQIA
EEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHH
KLKDDGTISGGMIPKLDTCVHAVEAGCEAAVVLDGRVPHAMLLEFFTARGAGTLIRADD
HCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure 
TATEKTELAKAEVLIEALPYFQRYAGRTFVVKYGGHAMGDPKAAREFAEDIVLLKAVGI
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHEECCC
NPVVVHGGGPQIGAMLKRLGVESTFVDGLRVTDKATAEVAEMVLSGAINKELVGWIAQAG
CEEEEECCCCHHHHHHHHHCCCHHHHCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCC
GKAMGVSGKDGSLVTATKLERTTRDPESQIEQILDLGFVGEPTHVDTTILDTAVSAGMIP
CCEEECCCCCCCEEEHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCE
VVAPIGAGEDGHTYNINADTMAGAIAAALGAARLFLLTAVKGVLDKQGGLLTDLTPAQIA
EEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHH
KLKDDGTISGGMIPKLDTCVHAVEAGCEAAVVLDGRVPHAMLLEFFTARGAGTLIRADD
HCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA