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The map label for this gene is pbpG [H]

Identifier: 85375096

GI number: 85375096

Start: 2295336

End: 2297507

Strand: Reverse

Name: pbpG [H]

Synonym: ELI_11345

Alternate gene names: 85375096

Gene position: 2297507-2295336 (Counterclockwise)

Preceding gene: 85375097

Following gene: 85375092

Centisome position: 75.27

GC content: 64.46

Gene sequence:

>2172_bases
ATGGCCAAACGCGGCAGCCGCCGGCAAGCGGCGCAGAAAGCCAGGAAAGCAGCGGCAGGCAAGCAGCCCGGCACCTTCGG
TCGCTGGGTGCGCCGCATCGCCCTCTGGGGCACGGCTATCGCCGTCCTCGGCGCGCTGTTCGTGGGTCTCGCCGTCCTGT
TCGCAGCACGATCGCTTCCCAGCTATTCGCAGTTGAAGGCGACCCAGAACGCGCAGACTATCGTCGTGCGCGCGCGCGAC
GGGACGGAAATCCTCGAGCTCGGCCCCAGCTTCGGCGAGTGGCTCGATCACGACGAAATTCCGCAAGTGATGAAGGACGC
GATGATCTCGGTCGAGGACCGTCGCTATTATTCGCATTTCGGTGTCGATCCCTACGGACTCGCGCGAGCGGTCTATACCG
CCGCGACGGGCAATGGGCGTATATCGGCGACGTCGACCATCACTCAGCAGCTGGCGCGCAATATCTTCCTCAATTCCAAT
CGCACGGTCGATCGCAAACTGCGAGAAGGCGTGCTCGCGCTGGCACTGGAAACGAAGTTTTCGAAGGAACAGATCCTCGA
ACTCTATCTCAACAAGGTCTACTTCGGTGGCGGCGCCTACGGGATCGACAGCGCGAGCCGTAAATTTTTCAGCCATCCCG
CCAGCGAGCTTACCGTCGCGGAAGCTGCGATCATCGCAGGTCTGGTCAAGGCCCCAAGCCGCTATTCGCCGACTGCCGAT
GTCGACGCCGCCGTCGGACGGGCCAAGACCGTGCTGCGGCTGATGCGCGAACAGGGCCGCATTACCGCAAGCCAGGCTTC
GGTCGATGTCGATGCCGTCAATCTCAAGCAGGAAGCGGGACAGAACTCCGTCCGCTATTTCACCGACTGGGCGCTACCGC
AGCTCGATATCCTGTTGCCCGAAACTTTCGAGCCGATCACGGTCTGGACCACGCTCGATGTCGGCATGCAGCGCGCGGCG
CAGACCTCGATAGAGGCCAATACGCCGAACGGGGCGCAGGGTGCACTCGTCAGCATGGATCGCGACGGTGCGATTCTCGC
GCTCGTTGGCGGCAGCGACTATGTCGAGACGAGTTTCAACCGCGCGACGACGGCACTGCGCCAGCCGGGTTCCGCCTGGA
AACTGTTCGTCTATCTCGCCGCGCTGGAAGCCGGCTACACCCCGGAAGATCGCGTCGTCGATACACCGGTCACGATAGAC
GGGTGGAGCCCGCGCAATTCAGGCGGCGGCTATTCGGGCGAGATGAACCTGCGGACCGCTTTTGCCTATTCGAAGAACAC
CGTCGCCGCGCAGCTCGGCAACGAGGTCGGCTTCGGTACGGTCGCGTCGATGGCGCGCCGGTTCGGGATCACTACAGAGA
TTTCGACCTTCCCGGCCATGGTGCTGGGATCGAGCGAAGTCCGTCTGATCGACATGACCCGCGCGTTCGCTGCGGTGTCG
GCCAAGGGCATGAGCGTCGAGCCTTACGGCATCGTCAAGGTGACGACTTCCGACGGCGAGACGCTGTATCAGCGCGAGAA
GCCGCGCGAGGTGAAGCTGGTGCCGGATTATGTCGCCGCCGGTATGACCGACCTGTTGCAGGCGGCGGTCGCCACCGGCA
CCGGTCGCGCCGCCCAGATCGGGCGCCCCGTGGCCGGCAAGACCGGCACGACCAGCTCCAACAAGGATGGCTATTTCGTC
GGCTTCTCCAGCGGCATCACAACCGGCGTTTGGATGGGCCGCGACGACAACCGGGCCGTCGGTGGTTTGCAGGGAGGTCG
CGCACCGGCGCAGGCCTTTGCCGCCTTCATGCGCTTCGCGGTGAAGGATCGCCCGCCGGAAAAATTCGAGACCGACCTGC
AATTGCCGGAATGGCAGCTCGAACCCGACGACGAATTCTATTTCGGCGATCCGGACGACTATTATTATATCGACGAGCAG
GGGAACCTGATCGAACCGGGACGGGTCGAAGGCCCGCGCGGCGACGGTTTCCCGGTCGAAGGCGAAACAGCGCCTGGCCC
GGCCAACCGTACGCCGCCGCCCGATCCGGCGCGCGATGGCGCTCCGCAGGCGGCCAGCGACGACTTTCTCGAACGTGCCA
CCGGAGGCGACAACGAGCAGCGTTCGCCGCCGCCCCGGCAGCCTGCGCAAAGGCCCCAGCCGCCGCCCGGCACAAATTCG
CAGAACCGATAG

Upstream 100 bases:

>100_bases
ACAGTGCCCATTCACTGCCGGTTAGCCTTCGGCCGTCCATGGGTTATAGGGGAACGGGTGCGCGGCCTAAGCGCGCGGGT
GACAGGATAGTACGGAACTG

Downstream 100 bases:

>100_bases
AAAAAGCCCCGCCGGATCGCTCCGACGGGGCTCTTTTTTGCTCGGTGCAATCTGTTAGCGGAAGCGCACTGCGCGGAAGG
CCGTCTGCCCGCCGCGGCGG

Product: membrane carboxypeptidase

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MAKRGSRRQAAQKARKAAAGKQPGTFGRWVRRIALWGTAIAVLGALFVGLAVLFAARSLPSYSQLKATQNAQTIVVRARD
GTEILELGPSFGEWLDHDEIPQVMKDAMISVEDRRYYSHFGVDPYGLARAVYTAATGNGRISATSTITQQLARNIFLNSN
RTVDRKLREGVLALALETKFSKEQILELYLNKVYFGGGAYGIDSASRKFFSHPASELTVAEAAIIAGLVKAPSRYSPTAD
VDAAVGRAKTVLRLMREQGRITASQASVDVDAVNLKQEAGQNSVRYFTDWALPQLDILLPETFEPITVWTTLDVGMQRAA
QTSIEANTPNGAQGALVSMDRDGAILALVGGSDYVETSFNRATTALRQPGSAWKLFVYLAALEAGYTPEDRVVDTPVTID
GWSPRNSGGGYSGEMNLRTAFAYSKNTVAAQLGNEVGFGTVASMARRFGITTEISTFPAMVLGSSEVRLIDMTRAFAAVS
AKGMSVEPYGIVKVTTSDGETLYQREKPREVKLVPDYVAAGMTDLLQAAVATGTGRAAQIGRPVAGKTGTTSSNKDGYFV
GFSSGITTGVWMGRDDNRAVGGLQGGRAPAQAFAAFMRFAVKDRPPEKFETDLQLPEWQLEPDDEFYFGDPDDYYYIDEQ
GNLIEPGRVEGPRGDGFPVEGETAPGPANRTPPPDPARDGAPQAASDDFLERATGGDNEQRSPPPRQPAQRPQPPPGTNS
QNR

Sequences:

>Translated_723_residues
MAKRGSRRQAAQKARKAAAGKQPGTFGRWVRRIALWGTAIAVLGALFVGLAVLFAARSLPSYSQLKATQNAQTIVVRARD
GTEILELGPSFGEWLDHDEIPQVMKDAMISVEDRRYYSHFGVDPYGLARAVYTAATGNGRISATSTITQQLARNIFLNSN
RTVDRKLREGVLALALETKFSKEQILELYLNKVYFGGGAYGIDSASRKFFSHPASELTVAEAAIIAGLVKAPSRYSPTAD
VDAAVGRAKTVLRLMREQGRITASQASVDVDAVNLKQEAGQNSVRYFTDWALPQLDILLPETFEPITVWTTLDVGMQRAA
QTSIEANTPNGAQGALVSMDRDGAILALVGGSDYVETSFNRATTALRQPGSAWKLFVYLAALEAGYTPEDRVVDTPVTID
GWSPRNSGGGYSGEMNLRTAFAYSKNTVAAQLGNEVGFGTVASMARRFGITTEISTFPAMVLGSSEVRLIDMTRAFAAVS
AKGMSVEPYGIVKVTTSDGETLYQREKPREVKLVPDYVAAGMTDLLQAAVATGTGRAAQIGRPVAGKTGTTSSNKDGYFV
GFSSGITTGVWMGRDDNRAVGGLQGGRAPAQAFAAFMRFAVKDRPPEKFETDLQLPEWQLEPDDEFYFGDPDDYYYIDEQ
GNLIEPGRVEGPRGDGFPVEGETAPGPANRTPPPDPARDGAPQAASDDFLERATGGDNEQRSPPPRQPAQRPQPPPGTNS
QNR
>Mature_722_residues
AKRGSRRQAAQKARKAAAGKQPGTFGRWVRRIALWGTAIAVLGALFVGLAVLFAARSLPSYSQLKATQNAQTIVVRARDG
TEILELGPSFGEWLDHDEIPQVMKDAMISVEDRRYYSHFGVDPYGLARAVYTAATGNGRISATSTITQQLARNIFLNSNR
TVDRKLREGVLALALETKFSKEQILELYLNKVYFGGGAYGIDSASRKFFSHPASELTVAEAAIIAGLVKAPSRYSPTADV
DAAVGRAKTVLRLMREQGRITASQASVDVDAVNLKQEAGQNSVRYFTDWALPQLDILLPETFEPITVWTTLDVGMQRAAQ
TSIEANTPNGAQGALVSMDRDGAILALVGGSDYVETSFNRATTALRQPGSAWKLFVYLAALEAGYTPEDRVVDTPVTIDG
WSPRNSGGGYSGEMNLRTAFAYSKNTVAAQLGNEVGFGTVASMARRFGITTEISTFPAMVLGSSEVRLIDMTRAFAAVSA
KGMSVEPYGIVKVTTSDGETLYQREKPREVKLVPDYVAAGMTDLLQAAVATGTGRAAQIGRPVAGKTGTTSSNKDGYFVG
FSSGITTGVWMGRDDNRAVGGLQGGRAPAQAFAAFMRFAVKDRPPEKFETDLQLPEWQLEPDDEFYFGDPDDYYYIDEQG
NLIEPGRVEGPRGDGFPVEGETAPGPANRTPPPDPARDGAPQAASDDFLERATGGDNEQRSPPPRQPAQRPQPPPGTNSQ
NR

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=510, Percent_Identity=30.9803921568627, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI87082258, Length=306, Percent_Identity=34.3137254901961, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1788867, Length=529, Percent_Identity=28.7334593572779, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789601, Length=167, Percent_Identity=35.9281437125749, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 77912; Mature: 77781

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRGSRRQAAQKARKAAAGKQPGTFGRWVRRIALWGTAIAVLGALFVGLAVLFAARSLP
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SYSQLKATQNAQTIVVRARDGTEILELGPSFGEWLDHDEIPQVMKDAMISVEDRRYYSHF
CHHHHHHCCCCEEEEEEECCCCHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GVDPYGLARAVYTAATGNGRISATSTITQQLARNIFLNSNRTVDRKLREGVLALALETKF
CCCHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEHHCCC
SKEQILELYLNKVYFGGGAYGIDSASRKFFSHPASELTVAEAAIIAGLVKAPSRYSPTAD
CHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
VDAAVGRAKTVLRLMREQGRITASQASVDVDAVNLKQEAGQNSVRYFTDWALPQLDILLP
HHHHHHHHHHHHHHHHHCCCEEECCCCCCEEHHHHHHHCCCCCCEEEECCCCCCEEEECC
ETFEPITVWTTLDVGMQRAAQTSIEANTPNGAQGALVSMDRDGAILALVGGSDYVETSFN
CCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCHHHHHHH
RATTALRQPGSAWKLFVYLAALEAGYTPEDRVVDTPVTIDGWSPRNSGGGYSGEMNLRTA
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEECCCEEECCCCCCCCCCCCCCCEEEEEE
FAYSKNTVAAQLGNEVGFGTVASMARRFGITTEISTFPAMVLGSSEVRLIDMTRAFAAVS
EEECCCCHHHHHCCCCCHHHHHHHHHHHCCCEEHHHCCHHEECCCCEEEEEHHHHHHHHH
AKGMSVEPYGIVKVTTSDGETLYQREKPREVKLVPDYVAAGMTDLLQAAVATGTGRAAQI
CCCCCCCCCEEEEEECCCCHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCHHHC
GRPVAGKTGTTSSNKDGYFVGFSSGITTGVWMGRDDNRAVGGLQGGRAPAQAFAAFMRFA
CCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
VKDRPPEKFETDLQLPEWQLEPDDEFYFGDPDDYYYIDEQGNLIEPGRVEGPRGDGFPVE
HCCCCCHHHCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCEECCCCCCCCCCCCCCCC
GETAPGPANRTPPPDPARDGAPQAASDDFLERATGGDNEQRSPPPRQPAQRPQPPPGTNS
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
QNR
CCC
>Mature Secondary Structure 
AKRGSRRQAAQKARKAAAGKQPGTFGRWVRRIALWGTAIAVLGALFVGLAVLFAARSLP
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SYSQLKATQNAQTIVVRARDGTEILELGPSFGEWLDHDEIPQVMKDAMISVEDRRYYSHF
CHHHHHHCCCCEEEEEEECCCCHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GVDPYGLARAVYTAATGNGRISATSTITQQLARNIFLNSNRTVDRKLREGVLALALETKF
CCCHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEHHCCC
SKEQILELYLNKVYFGGGAYGIDSASRKFFSHPASELTVAEAAIIAGLVKAPSRYSPTAD
CHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
VDAAVGRAKTVLRLMREQGRITASQASVDVDAVNLKQEAGQNSVRYFTDWALPQLDILLP
HHHHHHHHHHHHHHHHHCCCEEECCCCCCEEHHHHHHHCCCCCCEEEECCCCCCEEEECC
ETFEPITVWTTLDVGMQRAAQTSIEANTPNGAQGALVSMDRDGAILALVGGSDYVETSFN
CCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCHHHHHHH
RATTALRQPGSAWKLFVYLAALEAGYTPEDRVVDTPVTIDGWSPRNSGGGYSGEMNLRTA
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEECCCEEECCCCCCCCCCCCCCCEEEEEE
FAYSKNTVAAQLGNEVGFGTVASMARRFGITTEISTFPAMVLGSSEVRLIDMTRAFAAVS
EEECCCCHHHHHCCCCCHHHHHHHHHHHCCCEEHHHCCHHEECCCCEEEEEHHHHHHHHH
AKGMSVEPYGIVKVTTSDGETLYQREKPREVKLVPDYVAAGMTDLLQAAVATGTGRAAQI
CCCCCCCCCEEEEEECCCCHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCHHHC
GRPVAGKTGTTSSNKDGYFVGFSSGITTGVWMGRDDNRAVGGLQGGRAPAQAFAAFMRFA
CCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
VKDRPPEKFETDLQLPEWQLEPDDEFYFGDPDDYYYIDEQGNLIEPGRVEGPRGDGFPVE
HCCCCCHHHCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCEECCCCCCCCCCCCCCCC
GETAPGPANRTPPPDPARDGAPQAASDDFLERATGGDNEQRSPPPRQPAQRPQPPPGTNS
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
QNR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]