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The map label for this gene is rsmB [H]

Identifier: 85375086

GI number: 85375086

Start: 2283819

End: 2285066

Strand: Reverse

Name: rsmB [H]

Synonym: ELI_11295

Alternate gene names: 85375086

Gene position: 2285066-2283819 (Counterclockwise)

Preceding gene: 85375088

Following gene: 85375084

Centisome position: 74.86

GC content: 68.91

Gene sequence:

>1248_bases
ATGGCCCAACCGACAGGAATCCATGCGCGCCGCGCTGCCTTGCAGATGCTCGATGCCGTCTTGCGGCGGGGTGAGACGCT
GGAGCAGGCCGAAGGTGCCGCGCTGCGGCAGGTCCGCAGCGCGCCCGACAAGGCGCTCGCGCGCGCCATCGCCAACGAGA
CGTTGCGCTGGCTCACCGATTTCGATGCGCTGATCGACAGCGCGACGAAACAACGGCTGCCCGATGATGCCAAGGCGCGG
ACGGTCTTGCGGTTGATGCTGGCGCAATGGCTGCGGCTGGAGACGCCGCCGCATGCCGTGATCGCCACCGGGCTCCCCCT
GCTGGCGGGCGGACCGCGCCGGCTGGCGCACGGGGTATTCTCGACACTGACGAAGAAAGAGGTGCGCCTGCCCGCCGCAC
CGACCTTGCCGGTGCGGGTGAGGGAGCGCTGGGGCGACCGCGCCGATGAAATCGCCGCGGGTCTGGCCGAGCCGCCGCCG
CTGGACTTGACCCTGCGCGATGCTGGCGAAACGCAGCACTGGGCCAACCAGCTCGCCGCCGATAGCTACGCCCCCGGCCA
TCTGCGCCTGCCGCGCGGGTCGGCTGTCGAAACGCTGGCCGGGTTCAGGGAAGGCGCGTGGTGGGTGCAGGATCTCGCTG
CGTCGATCCCGGCGCGACTGCTCGGGCCGGGCGAGGGGCGGCACGTGCTCGACCTGTGCGCCGCGCCCGGTGGCAAGACG
CTCCAGCTCGCCGCCGCAGGCTGGAAAGTCACGGCGCTAGACATCAGCAAGCGGCGGCTCGGCCTGCTCAAGGACAATCT
CACGCGCACGAACCTGAAGGCGTCGACCGTTCGCGCCGATGCTCTAGCCTGGGAACCGAAGCATCACTTCGACGCCATCC
TGCTCGACGCACCCTGCACCGCCACCGGCACCTGCCGCCGCCATCCCGATGTCCTGCACCGCATCGGCCCGCGTCAGATC
GGGGAGATGAGTGAGCTGCAGGCGGCGCTGCTGGGTCGCGCGCAGGGCTGGCTCAAGCCGGGCGGGACCCTAGTCTATGC
GGTGTGTTCGCTGGAAGAAGAAGAGGGCAAGGTTCAGGCTGCCAAGGTCGTTCTGACCCCGGAGCCGGTCGCGCCAGGGG
AATTGCCATCCGGCCTGATGCCAAGCGACGAAGGATGGGTGCGGACACATCCGGGAATGCTCTGCACACAAGGCGGTCTC
GACGGATTCTTTATCGCGCGGTGGCGAAAAGCTCCGGACGATGTGTAA

Upstream 100 bases:

>100_bases
GGGCGGGCTTCTTGAAGTCCCTCGGGCGTTGGGTTGCGCGCTTCATATTCTTGAAATGCGCGCACTGGCGCTTGGTTTCA
AGCTGGCCTAACGGCGGGGC

Downstream 100 bases:

>100_bases
GCATCCGGCTTAAGTTAGGTAACGGGGTCGCGCATCAGGCCGAGCACCGACCGAAGCTGGATGGCGAAGTCTATTCGGCA
AGACCCTCGCCCAGATCATA

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MAQPTGIHARRAALQMLDAVLRRGETLEQAEGAALRQVRSAPDKALARAIANETLRWLTDFDALIDSATKQRLPDDAKAR
TVLRLMLAQWLRLETPPHAVIATGLPLLAGGPRRLAHGVFSTLTKKEVRLPAAPTLPVRVRERWGDRADEIAAGLAEPPP
LDLTLRDAGETQHWANQLAADSYAPGHLRLPRGSAVETLAGFREGAWWVQDLAASIPARLLGPGEGRHVLDLCAAPGGKT
LQLAAAGWKVTALDISKRRLGLLKDNLTRTNLKASTVRADALAWEPKHHFDAILLDAPCTATGTCRRHPDVLHRIGPRQI
GEMSELQAALLGRAQGWLKPGGTLVYAVCSLEEEEGKVQAAKVVLTPEPVAPGELPSGLMPSDEGWVRTHPGMLCTQGGL
DGFFIARWRKAPDDV

Sequences:

>Translated_415_residues
MAQPTGIHARRAALQMLDAVLRRGETLEQAEGAALRQVRSAPDKALARAIANETLRWLTDFDALIDSATKQRLPDDAKAR
TVLRLMLAQWLRLETPPHAVIATGLPLLAGGPRRLAHGVFSTLTKKEVRLPAAPTLPVRVRERWGDRADEIAAGLAEPPP
LDLTLRDAGETQHWANQLAADSYAPGHLRLPRGSAVETLAGFREGAWWVQDLAASIPARLLGPGEGRHVLDLCAAPGGKT
LQLAAAGWKVTALDISKRRLGLLKDNLTRTNLKASTVRADALAWEPKHHFDAILLDAPCTATGTCRRHPDVLHRIGPRQI
GEMSELQAALLGRAQGWLKPGGTLVYAVCSLEEEEGKVQAAKVVLTPEPVAPGELPSGLMPSDEGWVRTHPGMLCTQGGL
DGFFIARWRKAPDDV
>Mature_414_residues
AQPTGIHARRAALQMLDAVLRRGETLEQAEGAALRQVRSAPDKALARAIANETLRWLTDFDALIDSATKQRLPDDAKART
VLRLMLAQWLRLETPPHAVIATGLPLLAGGPRRLAHGVFSTLTKKEVRLPAAPTLPVRVRERWGDRADEIAAGLAEPPPL
DLTLRDAGETQHWANQLAADSYAPGHLRLPRGSAVETLAGFREGAWWVQDLAASIPARLLGPGEGRHVLDLCAAPGGKTL
QLAAAGWKVTALDISKRRLGLLKDNLTRTNLKASTVRADALAWEPKHHFDAILLDAPCTATGTCRRHPDVLHRIGPRQIG
EMSELQAALLGRAQGWLKPGGTLVYAVCSLEEEEGKVQAAKVVLTPEPVAPGELPSGLMPSDEGWVRTHPGMLCTQGGLD
GFFIARWRKAPDDV

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=252, Percent_Identity=30.5555555555556, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI76150623, Length=252, Percent_Identity=30.5555555555556, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI23199998, Length=191, Percent_Identity=32.4607329842932, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI270288818, Length=191, Percent_Identity=32.4607329842932, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI270288816, Length=191, Percent_Identity=32.4607329842932, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI8922322, Length=191, Percent_Identity=32.4607329842932, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI2367212, Length=433, Percent_Identity=33.0254041570439, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI87081985, Length=236, Percent_Identity=33.4745762711864, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17536757, Length=243, Percent_Identity=29.6296296296296, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25143471, Length=172, Percent_Identity=30.8139534883721, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=245, Percent_Identity=31.0204081632653, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI22024126, Length=255, Percent_Identity=27.0588235294118, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24668781, Length=190, Percent_Identity=32.1052631578947, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356513, Length=190, Percent_Identity=32.1052631578947, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355201, Length=201, Percent_Identity=29.8507462686567, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 44865; Mature: 44734

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPTGIHARRAALQMLDAVLRRGETLEQAEGAALRQVRSAPDKALARAIANETLRWLTD
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
FDALIDSATKQRLPDDAKARTVLRLMLAQWLRLETPPHAVIATGLPLLAGGPRRLAHGVF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHCCCHHHHHHHHH
STLTKKEVRLPAAPTLPVRVRERWGDRADEIAAGLAEPPPLDLTLRDAGETQHWANQLAA
HHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHC
DSYAPGHLRLPRGSAVETLAGFREGAWWVQDLAASIPARLLGPGEGRHVLDLCAAPGGKT
CCCCCCCEECCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCEEHHHHCCCCCCE
LQLAAAGWKVTALDISKRRLGLLKDNLTRTNLKASTVRADALAWEPKHHFDAILLDAPCT
EEEEECCCEEEEEECCHHHHHHHHCCCHHCCCCHHHEEHHHEECCCCCCCCEEEEECCCC
ATGTCRRHPDVLHRIGPRQIGEMSELQAALLGRAQGWLKPGGTLVYAVCSLEEEEGKVQA
CCCCCCCCHHHHHHCCCHHHCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHCCCEEE
AKVVLTPEPVAPGELPSGLMPSDEGWVRTHPGMLCTQGGLDGFFIARWRKAPDDV
EEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCEEECCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
AQPTGIHARRAALQMLDAVLRRGETLEQAEGAALRQVRSAPDKALARAIANETLRWLTD
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
FDALIDSATKQRLPDDAKARTVLRLMLAQWLRLETPPHAVIATGLPLLAGGPRRLAHGVF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHCCCHHHHHHHHH
STLTKKEVRLPAAPTLPVRVRERWGDRADEIAAGLAEPPPLDLTLRDAGETQHWANQLAA
HHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHC
DSYAPGHLRLPRGSAVETLAGFREGAWWVQDLAASIPARLLGPGEGRHVLDLCAAPGGKT
CCCCCCCEECCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCEEHHHHCCCCCCE
LQLAAAGWKVTALDISKRRLGLLKDNLTRTNLKASTVRADALAWEPKHHFDAILLDAPCT
EEEEECCCEEEEEECCHHHHHHHHCCCHHCCCCHHHEEHHHEECCCCCCCCEEEEECCCC
ATGTCRRHPDVLHRIGPRQIGEMSELQAALLGRAQGWLKPGGTLVYAVCSLEEEEGKVQA
CCCCCCCCHHHHHHCCCHHHCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHCCCEEE
AKVVLTPEPVAPGELPSGLMPSDEGWVRTHPGMLCTQGGLDGFFIARWRKAPDDV
EEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCEEECCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA