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The map label for this gene is azoR

Identifier: 85374506

GI number: 85374506

Start: 1717909

End: 1718502

Strand: Direct

Name: azoR

Synonym: ELI_08395

Alternate gene names: 85374506

Gene position: 1717909-1718502 (Clockwise)

Preceding gene: 85374505

Following gene: 85374510

Centisome position: 56.28

GC content: 63.64

Gene sequence:

>594_bases
ATGTCCACCATCCTTCACATCACCGCCAGCATCCGCGGCGACGAATCGATTTCGCGCGCGCTCAGCTCCAAGCTGGTCGA
GCGCCTCTCTGGGACCGACACCAAGGTCATCACGCGTGACCTGTCGCAAAACGACATTCCCTATGTCGATGCCGACCGCT
TCGCCGCCAACCTGTCGCCCTATACCGAGCGCTCGCCCGAGCAGCAGGAACTGGCCGCCATCGCCGATACGCTGATCGAA
GAATTGCAGGCGGCCGATACCATCGTCCTTGGCGTACCGATCTACAACTTCTCCGTCCCCGCCACCGTCAAGGCCTGGGC
CGACACGGTCGCCCGCGCGGGCACGACGTTCGAATACACCCCGACCGGGCCGAAGGGTAAGCTGGACGGGAAGAAAGCCT
ACATCACCGTCGCATCGGGCGGCACGCCGGTCGGCAGCGAAGTCGACTTCATGAGCCCGTGGTTGAAATTCTTCCTCGGC
TTCCTCGGTATCTCCGAGGTCGAAGTGGTCGCGACCGCCGACGGCATCATGGGCGAAGGCGGCGAAGAGAAGATCGCCTC
CGCGCACAAACAGGTCGAGCAGGTCGCGGCCTGA

Upstream 100 bases:

>100_bases
CTCGAGGACAGCGAACTCGTCCTCGTCGACGCGGCCTGACCCCCGGCCTCGCCCTCACCCCCGAGGGCGGGGCATTTCAT
TTCCAAGCAGGAGAATTTCC

Downstream 100 bases:

>100_bases
TCGACCCAGCGCAGGCGCCAAGGCCGCGACGTTCCCGCCACCGGGGGCGTCGCGGCTACAGCCTCGGCCGGACCAGCAGC
GGCTTGAAGATCGTGCCGCT

Product: FMN-dependent NADH-azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSTILHITASIRGDESISRALSSKLVERLSGTDTKVITRDLSQNDIPYVDADRFAANLSPYTERSPEQQELAAIADTLIE
ELQAADTIVLGVPIYNFSVPATVKAWADTVARAGTTFEYTPTGPKGKLDGKKAYITVASGGTPVGSEVDFMSPWLKFFLG
FLGISEVEVVATADGIMGEGGEEKIASAHKQVEQVAA

Sequences:

>Translated_197_residues
MSTILHITASIRGDESISRALSSKLVERLSGTDTKVITRDLSQNDIPYVDADRFAANLSPYTERSPEQQELAAIADTLIE
ELQAADTIVLGVPIYNFSVPATVKAWADTVARAGTTFEYTPTGPKGKLDGKKAYITVASGGTPVGSEVDFMSPWLKFFLG
FLGISEVEVVATADGIMGEGGEEKIASAHKQVEQVAA
>Mature_196_residues
STILHITASIRGDESISRALSSKLVERLSGTDTKVITRDLSQNDIPYVDADRFAANLSPYTERSPEQQELAAIADTLIEE
LQAADTIVLGVPIYNFSVPATVKAWADTVARAGTTFEYTPTGPKGKLDGKKAYITVASGGTPVGSEVDFMSPWLKFFLGF
LGISEVEVVATADGIMGEGGEEKIASAHKQVEQVAA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=178, Percent_Identity=40.4494382022472, Blast_Score=126, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR_ERYLH (Q2N970)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458568.1
- HSSP:   P41407
- ProteinModelPortal:   Q2N970
- SMR:   Q2N970
- STRING:   Q2N970
- GeneID:   3870918
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_08395
- NMPDR:   fig|314225.3.peg.1363
- eggNOG:   COG1182
- HOGENOM:   HBG565834
- OMA:   MTTILRI
- PhylomeDB:   Q2N970
- BioCyc:   ELIT314225:ELI_08395-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 20997; Mature: 20866

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILHITASIRGDESISRALSSKLVERLSGTDTKVITRDLSQNDIPYVDADRFAANLSP
CCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCCHHHHHHCCCC
YTERSPEQQELAAIADTLIEELQAADTIVLGVPIYNFSVPATVKAWADTVARAGTTFEYT
CCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECEEECCCCCCHHHHHHHHHHHCCCCEEEC
PTGPKGKLDGKKAYITVASGGTPVGSEVDFMSPWLKFFLGFLGISEVEVVATADGIMGEG
CCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GEEKIASAHKQVEQVAA
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STILHITASIRGDESISRALSSKLVERLSGTDTKVITRDLSQNDIPYVDADRFAANLSP
CCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCCHHHHHHCCCC
YTERSPEQQELAAIADTLIEELQAADTIVLGVPIYNFSVPATVKAWADTVARAGTTFEYT
CCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECEEECCCCCCHHHHHHHHHHHCCCCEEEC
PTGPKGKLDGKKAYITVASGGTPVGSEVDFMSPWLKFFLGFLGISEVEVVATADGIMGEG
CCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GEEKIASAHKQVEQVAA
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA